Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 78,830 T→A 15.3% intergenic (+31/+18) setA → / ← leuD broad specificity sugar efflux system/3‑isopropylmalate dehydratase small subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091378,8300TA15.3% 292.3 / 43.4 118intergenic (+31/+18)setA/leuDbroad specificity sugar efflux system/3‑isopropylmalate dehydratase small subunit
Reads supporting (aligned to +/- strand):  ref base T (54/45);  new base A (8/10);  total (62/56)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

Only 100 of 121 total aligned reads displayed.

CTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGA  >  NC_000913/78733‑78929
                                                                                                 |                                                                                                   
cTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAttt                                                                                                   >  1:1272297/1‑100 (MQ=255)
cTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAttt                                                                                                   <  2:528174/100‑1 (MQ=255)
 tACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTg                                                                                                  <  1:848994/100‑1 (MQ=255)
 tACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTg                                                                                                  <  1:710824/100‑1 (MQ=255)
 tACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTg                                                                                                  >  1:1422688/1‑100 (MQ=255)
 tACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTg                                                                                                  <  1:1879775/100‑1 (MQ=255)
 tACTCGTTAATAGCGGTTATTTCTTTTTTCGCATTATTTTTAATCGCAAAGGTTAAAGACGTTTGATGACGTGTACGATAGCGGTCAGCCCGGTCTTTTg                                                                                                  <  2:1709481/100‑1 (MQ=255)
  aCTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTAATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGa                                                                                                 >  2:1768733/1‑100 (MQ=255)
   cTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGAc                                                                                                >  2:2405441/1‑100 (MQ=255)
   cTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGAc                                                                                                <  2:1595501/100‑1 (MQ=255)
   cTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGAc                                                                                                <  2:1437484/100‑1 (MQ=255)
      ggTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCgg                                                                                             <  1:159609/100‑1 (MQ=255)
      ggTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCgg                                                                                             >  2:2513102/1‑100 (MQ=255)
       gTAGTTGCCGTGATTTCTGTTGTCGCTTTATTTTTACCCGCAAAGGTTAAAGCCGCTTGAGGTCGTGGGCGATAGCGGAAAGCCCGGTCATTTGGCCggg                                                                                            <  2:600188/100‑1 (MQ=255)
       gTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCggg                                                                                            <  2:335974/100‑1 (MQ=255)
        tAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGAGGATAGCGGAAAGCCCGGTCAAATGACCggtc                                                                                           <  1:1730168/100‑3 (MQ=255)
        tAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGc                                                                                           <  1:165927/100‑1 (MQ=255)
        tAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGc                                                                                           <  1:2472446/100‑1 (MQ=255)
        tAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGc                                                                                           <  1:937138/100‑1 (MQ=255)
        tAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGc                                                                                           <  1:665274/100‑1 (MQ=255)
        tAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGc                                                                                           <  1:448962/100‑1 (MQ=255)
         aaTTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGct                                                                                          <  1:1756576/100‑2 (MQ=255)
          aTTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCggg                                                                                            >  2:1100478/1‑97 (MQ=255)
           ttGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAg                                                                                        <  2:202868/100‑1 (MQ=255)
            tGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGctttc                                                                                       <  1:1769894/100‑5 (MQ=255)
             gCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCGAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAggg                                                                                      >  2:2003446/1‑100 (MQ=255)
             gCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAggg                                                                                      >  1:203413/1‑100 (MQ=255)
               ggTTATTTCTGTTGTCGCATTATTTTTAACCGCAACGGTTAAAGACGTTTGATGCCGTGGACGATAGCGGAACGCCCGGTCATTTGACCGGGCACgggg                                                                                     >  2:511295/1‑99 (MQ=255)
               ggTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGa                                                                                    >  2:1974430/1‑100 (MQ=255)
                  tATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATAACGTGGACGATACCGGAAAAAACGGTCATTTGACCGGGCAAGGGGATTa                                                                                 >  1:1493208/1‑100 (MQ=255)
                    tttCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAAt                                                                               >  2:812076/1‑100 (MQ=255)
                    tttCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAAt                                                                               >  1:2284641/1‑100 (MQ=255)
                     ttCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAttt                                                                              >  1:510225/1‑100 (MQ=255)
                            gtCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAAc                                                                       >  2:1269912/1‑100 (MQ=255)
                            gtCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAAc                                                                       <  1:2111668/100‑1 (MQ=255)
                               gCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCa                                                                    >  2:2346226/1‑100 (MQ=255)
                               gCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCa                                                                    >  1:363539/1‑100 (MQ=255)
                               gCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCa                                                                    >  2:1847926/1‑100 (MQ=255)
                                cATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAg                                                                   >  2:1950197/1‑100 (MQ=255)
                                cATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAg                                                                   >  2:1643198/1‑100 (MQ=255)
                                cATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAg                                                                   <  1:145438/100‑1 (MQ=255)
                                 nttattTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAgg                                                                  >  1:1930226/2‑100 (MQ=255)
                                 attattTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAgg                                                                  <  1:413226/100‑1 (MQ=255)
                                     tttttAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCACGGGGATTAATTCATAAACGCAGgttgt                                                              >  1:1876231/1‑100 (MQ=255)
                                     tttttAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGgttgt                                                              >  2:1639268/1‑100 (MQ=255)
                                     tttttAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGgttgt                                                              >  1:2026800/1‑100 (MQ=255)
                                     tttttAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGgttgt                                                              >  1:1892729/1‑100 (MQ=255)
                                     tttttAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGgttgt                                                              >  1:1253150/1‑100 (MQ=255)
                                     tttttAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGgttgt                                                              >  1:1570375/1‑100 (MQ=255)
                                      ttttAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGgttgtt                                                             <  1:2088685/100‑1 (MQ=255)
                                       tttAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGttt                                                            >  1:1587054/1‑100 (MQ=255)
                                          aaCCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGc                                                         >  2:2028035/1‑100 (MQ=255)
                                          aaCCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGc                                                         <  2:876554/100‑1 (MQ=255)
                                           aCCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAGAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCt                                                        >  2:1719280/1‑100 (MQ=255)
                                            ccGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCtt                                                       >  1:65151/1‑100 (MQ=255)
                                             cGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTc                                                      >  2:891470/1‑100 (MQ=255)
                                             cGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTc                                                      <  2:1522959/100‑1 (MQ=255)
                                             cGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTc                                                      <  2:810255/100‑1 (MQ=255)
                                              gCAAAGGTTAAAGACTTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCa                                                     <  1:1491535/100‑1 (MQ=255)
                                              gCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGgttgagatcggaag                                                     >  1:1217381/1‑90 (MQ=255)
                                              gCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCa                                                     <  2:2529897/100‑1 (MQ=255)
                                               cAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCAt                                                    >  1:971158/1‑100 (MQ=255)
                                                aaaGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATa                                                   <  2:551975/100‑1 (MQ=255)
                                                   ggTTAAAGACGGTTGAGGACGTGGAGGAGAGCGGAATGCCCGGTCAGGTGACCGGGCAAGGGCATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGc                                                <  2:1361994/100‑1 (MQ=255)
                                                    gTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCg                                               <  1:2412182/100‑1 (MQ=255)
                                                    gTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCg                                               <  2:827280/100‑1 (MQ=255)
                                                    gTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCg                                               <  2:827070/100‑1 (MQ=255)
                                                      tAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGc                                             >  2:1081987/1‑100 (MQ=255)
                                                      tAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGc                                             <  1:1391269/100‑1 (MQ=255)
                                                        aaGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCaa                                           >  2:1864444/1‑100 (MQ=255)
                                                         aGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAAt                                          <  1:1221312/100‑1 (MQ=255)
                                                           aCGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATgg                                        <  2:1080401/100‑1 (MQ=255)
                                                               ttGATGATGTGGACGTGAGGGGCACCCCCGGTTATGTGACCGCTAACGGCGATTAATTCATCGACGCTGGTTGTTTTGCTTCAAAAGCGGCAATAAcgtc                                    >  2:1139859/1‑100 (MQ=255)
                                                                      cGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAGCGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGtgc                             <  2:30297/100‑1 (MQ=255)
                                                                        tGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGtgctg                           <  1:669977/100‑1 (MQ=255)
                                                                        tGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGtgctg                           <  1:339820/100‑1 (MQ=255)
                                                                           aCGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCaa                        <  1:2032303/100‑1 (MQ=255)
                                                                            cGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAg                       >  1:2303443/1‑100 (MQ=255)
                                                                             gATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAgg                      >  1:2244014/1‑100 (MQ=255)
                                                                             gATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAgg                      <  1:463754/100‑1 (MQ=255)
                                                                                 gCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTaa                   >  2:2265530/1‑99 (MQ=255)
                                                                                  cGGAAAGCCCTGTCATTTTACCGGGCAAGGGGATTAATGCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCTTGCTGCAAGGTAAg                  <  1:847747/99‑1 (MQ=255)
                                                                                   cccttgCCCGGTCAAATGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGcc                >  1:1699411/6‑100 (MQ=255)
                                                                                    ccttgCCCGGTCAAATGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTGTTGCTTCATAAGAGGCAATGACGTAGTCGTGCTGTAAGGTAAGgcc               >  2:2339508/5‑100 (MQ=255)
                                                                                      ttgCCCGGTCAAATGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCaa             >  1:744289/3‑100 (MQ=255)
                                                                                      ttgCCCGGTCAAATGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTGTTGCTTCATAAGCGGCAGTGGAGTCGTCGTGCTGTAAGGTAAGccg               >  2:2379829/3‑97 (MQ=255)
                                                                                       tgCCCGGTCAAATGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAAt            >  1:959805/2‑100 (MQ=255)
                                                                                       aGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAAt            >  2:2069874/1‑100 (MQ=255)
                                                                                         cccGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATAc          <  2:1746774/100‑1 (MQ=255)
                                                                                         cccGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATAc          <  1:201577/100‑1 (MQ=255)
                                                                                         cccGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATAc          <  1:237820/100‑1 (MQ=255)
                                                                                         cccGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATAc          >  1:1087873/1‑100 (MQ=255)
                                                                                         cccGGTCAAATGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATAc          >  1:1424483/1‑100 (MQ=255)
                                                                                          ccGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACt         >  1:1378597/1‑100 (MQ=255)
                                                                                            ggTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGt       >  2:1902070/1‑100 (MQ=255)
                                                                                            ggTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGt       >  1:2000524/1‑100 (MQ=255)
                                                                                               cATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCa    >  2:1514932/1‑100 (MQ=255)
                                                                                                aTTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAGTGGCGTCGTCGAGCTGCAGGGTAAGCGCGATACTGTCCAg   >  2:1062662/1‑100 (MQ=255)
                                                                                                aTTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAg   <  2:177295/100‑1 (MQ=255)
                                                                                                 tttGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGa  >  2:121953/1‑100 (MQ=255)
                                                                                                 |                                                                                                   
CTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGA  >  NC_000913/78733‑78929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: