Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 860,151 A→G 17.3% intergenic (‑123/+23) ybiV ← / ← ybiW sugar phosphatase; fructose‑1‑P/ribose‑5‑P/glucose‑6‑P phosphatase/putative pyruvate formate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913860,1510AG17.3% 226.2 / 44.1 110intergenic (‑123/+23)ybiV/ybiWsugar phosphatase; fructose‑1‑P/ribose‑5‑P/glucose‑6‑P phosphatase/putative pyruvate formate lyase
Reads supporting (aligned to +/- strand):  ref base A (42/49);  new base G (6/13);  total (48/62)
Fisher's exact test for biased strand distribution p-value = 3.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.76e-02

Only 100 of 116 total aligned reads displayed.

GAAACCGTAACAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCGGAATAG  >  NC_000913/860052‑860249
                                                                                                   |                                                                                                  
gAAACCGTAACAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGcga                                                                                                    >  1:1127365/1‑100 (MQ=255)
 aaaCCGTAACAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGcgac                                                                                                   <  1:970803/100‑1 (MQ=255)
    ccGTAACAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCgacgac                                                                                                >  2:1105809/1‑100 (MQ=255)
    ccGTAACAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCAGCgacgac                                                                                                >  2:467890/1‑100 (MQ=255)
          cAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCgtcgcc                                                                                          <  2:579758/100‑1 (MQ=255)
          cAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACgcagcc                                                                                          >  2:2489249/1‑100 (MQ=255)
             tgtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCggg                                                                                       <  1:310285/100‑1 (MQ=255)
             tgtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCggg                                                                                       <  1:980689/100‑1 (MQ=255)
             tgtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCggg                                                                                       <  1:1214875/100‑1 (MQ=255)
             tgtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCggg                                                                                       <  1:698351/100‑1 (MQ=255)
             tgtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCggg                                                                                       <  2:1470053/100‑1 (MQ=255)
             tgtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCggg                                                                                       <  2:1019228/100‑1 (MQ=255)
             tgtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCggg                                                                                       >  1:1426597/1‑100 (MQ=255)
              gtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCgggn                                                                                      <  1:2483601/100‑2 (MQ=255)
              gtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCGGGc                                                                                      <  1:71025/100‑1 (MQ=255)
              gtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCGGGc                                                                                      <  2:233329/100‑1 (MQ=255)
              gtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCGGGc                                                                                      <  1:110388/100‑1 (MQ=255)
              gtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCGGGc                                                                                      <  2:80001/100‑1 (MQ=255)
              gtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGc                                                                                      <  2:2391333/100‑1 (MQ=255)
               taataaCAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCGGGct                                                                                     <  1:1920976/100‑2 (MQ=255)
                aataaCAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCg                                                                                    >  1:1720997/1‑100 (MQ=255)
                  taaCAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCgag                                                                                  <  1:783147/100‑1 (MQ=255)
                   aaCAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCgagg                                                                                 <  2:535280/100‑1 (MQ=255)
                   aaCAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCgagg                                                                                 <  2:782668/100‑1 (MQ=255)
                    aCAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCgagga                                                                                <  1:713864/100‑1 (MQ=255)
                     cAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCgaggag                                                                               <  2:216760/100‑1 (MQ=255)
                       aTGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGAt                                                                             <  1:440274/100‑1 (MQ=255)
                           gACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACa                                                                         >  1:1060719/1‑100 (MQ=255)
                           gACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACa                                                                         <  2:1170242/100‑1 (MQ=255)
                            aCGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAg                                                                        <  2:2029376/100‑1 (MQ=255)
                             cGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGc                                                                       >  1:231725/1‑100 (MQ=255)
                             cGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGc                                                                       >  1:770783/1‑100 (MQ=255)
                             cGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGc                                                                       >  1:1445502/1‑100 (MQ=255)
                               cAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCat                                                                     >  1:739117/1‑100 (MQ=255)
                               cAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGAAGACGAAGGGACGAGGAGATTAAAGAat                                                                     >  1:1955982/1‑100 (MQ=255)
                                  aggacaAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATg                                                                  <  1:576305/97‑1 (MQ=255)
                                   gCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGt                                                                 <  2:1616500/100‑1 (MQ=255)
                                    cacaAATTATATTTCGAATGAAAGTAAGGTTGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGtt                                                                <  1:875383/100‑1 (MQ=255)
                                    cacaAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGtt                                                                >  2:1774504/1‑100 (MQ=255)
                                    cacaAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGtt                                                                >  1:2072738/1‑100 (MQ=255)
                                     acaAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTc                                                               <  2:1359850/100‑1 (MQ=255)
                                     acaAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTc                                                               <  1:1076252/100‑1 (MQ=255)
                                      caAATTATATTTCGAATGAAAGTAAGGATGAACTTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCa                                                              >  2:822109/1‑100 (MQ=255)
                                        aaTTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGt                                                            >  2:700682/1‑100 (MQ=255)
                                        aaTTATAGTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAAGGATTTATCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGt                                                            <  2:1206732/100‑1 (MQ=255)
                                         aTTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTa                                                           <  2:2456423/100‑1 (MQ=255)
                                             tatTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTAc                                                          >  2:698621/1‑97 (MQ=255)
                                             tatTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACggg                                                       <  1:1516803/100‑1 (MQ=255)
                                              atgtCGAATGAAAGGAAGGATGAAATGGATGAGGTGAATGAGTTAGCCCGGCGACGGCGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGc                                                      <  2:1528288/97‑1 (MQ=255)
                                              atTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGc                                                      <  1:427130/100‑1 (MQ=255)
                                               tttCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCGCGGCGACGACGCAGCCGGGGCGAGGAGATTACAGCATATGTTCAGTACggg                                                       >  2:1374614/1‑98 (MQ=255)
                                               tttCGAATGAAAGTAAGGATGAAATTGATGAGGTGAATGATTTAGCACGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCg                                                     >  2:1596305/1‑100 (MQ=255)
                                               tttCGAATGAAAGTAAGGATGAAATTGATGAGGTGAATGATCTAGCCCGGCGACGCCGCCGCCGGGCCGTGGAGATTACAGCATATGTTCAGTACGGTCg                                                     >  2:1596366/1‑100 (MQ=255)
                                                 tcaaaTGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGTCGGGCCGAGGAGATTACAGCATATCTTCAGTACGGGCg                                                     <  2:1583069/95‑1 (MQ=255)
                                                 tCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCgat                                                   <  1:2373622/100‑1 (MQ=255)
                                                 tCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCgat                                                   <  1:1112521/100‑1 (MQ=255)
                                                  cGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCGGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCgatg                                                  <  2:441381/100‑1 (MQ=255)
                                                   gAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTCCGGGCgatg                                                  >  2:1845883/1‑99 (MQ=255)
                                                    aaTGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCgatgat                                                >  1:2216286/1‑100 (MQ=255)
                                                    aaTGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCgatgat                                                >  2:2182190/1‑100 (MQ=255)
                                                    aaTGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCgatgat                                                <  2:404515/100‑1 (MQ=255)
                                                    aaTGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTAAAGCATATGTTCAGTACGGGCgatgat                                                >  2:2198342/1‑100 (MQ=255)
                                                      tGAAAGTAAGGATGAACTTGATGATGTGAATGATCTCGCCCGACGACGACGCCGCCGGGACGAGGAGAATACAGCATATGTTCAGTACGGGcagtg                                                  >  2:2169327/1‑92 (MQ=255)
                                                      tGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGatat                                              >  1:2398486/1‑100 (MQ=255)
                                                        aaaGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCg                                            <  2:1886792/100‑1 (MQ=255)
                                                               ggATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAg                                     >  1:2276020/1‑100 (MQ=255)
                                                                  tGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCCTATGTTCAGTACGGGCGCTGATATCGTCCTGAGCg                                   >  2:2216131/1‑99 (MQ=255)
                                                                   gAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTc                                 >  2:581923/1‑100 (MQ=255)
                                                                   gAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTc                                 <  1:984448/100‑1 (MQ=255)
                                                                     aaTTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTg                               <  2:1115258/100‑1 (MQ=255)
                                                                       ttGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGga                             <  2:2276006/100‑1 (MQ=255)
                                                                       ttGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGga                             <  2:2261925/100‑1 (MQ=255)
                                                                          tccgatctaaTGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGgagag                          <  2:2534547/92‑1 (MQ=255)
                                                                           tgatgTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGgagaga                         >  1:2216556/1‑100 (MQ=255)
                                                                             atgTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAgc                       >  2:1993648/1‑100 (MQ=255)
                                                                              tgtgAATTATTGAGCGCGGCGACGACGCCTCCTGGCCGATGAGATTACAGCATATGGTCAGTACGGGCGATGATATCGTCCTTAGCGTCTGGAGAGAgcg                      <  2:106393/100‑1 (MQ=255)
                                                                              tgtgAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAgcg                      >  1:1199067/1‑100 (MQ=255)
                                                                              tgtgAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAgcg                      >  1:437042/1‑100 (MQ=255)
                                                                               gtgAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAgcgc                     >  1:753113/1‑100 (MQ=255)
                                                                               gtgAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAgcgc                     >  1:1644623/1‑100 (MQ=255)
                                                                                 gAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCgg                   <  1:707693/100‑1 (MQ=255)
                                                                                  aaTGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGagagagatcgga                  >  1:2534562/1‑94 (MQ=255)
                                                                                     gATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTgaa               <  1:787427/100‑1 (MQ=255)
                                                                                     gATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTgaa               >  1:2532008/1‑100 (MQ=255)
                                                                                     gATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTgaa               <  2:2531993/100‑1 (MQ=255)
                                                                                      aTTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTgaag              <  1:711222/100‑1 (MQ=255)
                                                                                       tttAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTgaaga             >  1:1372181/1‑100 (MQ=255)
                                                                                         tAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAc           >  1:1246472/1‑100 (MQ=255)
                                                                                         cggCCCGGCGGCGTCGTCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAc           >  1:1932246/3‑100 (MQ=255)
                                                                                           gCCCGGCGGCGTCGTCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAcgc         >  1:89138/1‑100 (MQ=255)
                                                                                           gCCCGGCGGCGTCGTCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAcgc         >  1:303483/1‑100 (MQ=255)
                                                                                           gCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAcgc         <  1:26073/100‑1 (MQ=255)
                                                                                            cccGGCGGCGTCGTCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAcgcg        >  1:866929/1‑100 (MQ=255)
                                                                                            cccGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAcgcg        >  2:726513/1‑100 (MQ=255)
                                                                                            cccGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGAGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAcgcg        <  2:1425673/100‑1 (MQ=255)
                                                                                             ccGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCgg       <  1:262273/100‑1 (MQ=255)
                                                                                             ccGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCgg       >  2:2062559/1‑100 (MQ=255)
                                                                                             ccGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCgg       >  1:517280/1‑100 (MQ=255)
                                                                                               ggCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCGGaa     <  1:1110625/100‑1 (MQ=255)
                                                                                                  gacgacGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCGGAATAg  >  2:1851662/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
GAAACCGTAACAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCGGAATAG  >  NC_000913/860052‑860249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: