Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 860,154 A→T 17.7% intergenic (‑126/+20) ybiV ← / ← ybiW sugar phosphatase; fructose‑1‑P/ribose‑5‑P/glucose‑6‑P phosphatase/putative pyruvate formate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913860,1540AT17.7% 233.6 / 49.2 110intergenic (‑126/+20)ybiV/ybiWsugar phosphatase; fructose‑1‑P/ribose‑5‑P/glucose‑6‑P phosphatase/putative pyruvate formate lyase
Reads supporting (aligned to +/- strand):  ref base A (41/48);  new base T (6/13);  total (48/62)
Fisher's exact test for biased strand distribution p-value = 3.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.11e-02

Only 100 of 115 total aligned reads displayed.

CCGTAACAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCC  >  NC_000913/860056‑860252
                                                                                                  |                                                                                                  
ccGTAACAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCAGCgacgac                                                                                                   >  2:467890/1‑100 (MQ=255)
      cAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCgtcgcc                                                                                             <  2:579758/100‑1 (MQ=255)
      cAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACgcagcc                                                                                             >  2:2489249/1‑100 (MQ=255)
         tgtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCggg                                                                                          <  2:1470053/100‑1 (MQ=255)
         tgtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCggg                                                                                          <  1:698351/100‑1 (MQ=255)
         tgtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCggg                                                                                          <  1:980689/100‑1 (MQ=255)
         tgtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCggg                                                                                          <  1:310285/100‑1 (MQ=255)
         tgtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCggg                                                                                          <  2:1019228/100‑1 (MQ=255)
         tgtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCggg                                                                                          >  1:1426597/1‑100 (MQ=255)
          gtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCgggn                                                                                         <  1:2483601/100‑2 (MQ=255)
          gtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCGGGc                                                                                         <  1:110388/100‑1 (MQ=255)
          gtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCGGGc                                                                                         <  2:80001/100‑1 (MQ=255)
          gtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCGGGc                                                                                         <  1:71025/100‑1 (MQ=255)
          gtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCGGGc                                                                                         <  2:233329/100‑1 (MQ=255)
          gtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGc                                                                                         <  2:2391333/100‑1 (MQ=255)
           taataaCAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCGGGct                                                                                        <  1:1920976/100‑2 (MQ=255)
           taataaCAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGcc                                                                                        >  1:92031/1‑100 (MQ=255)
            aataaCAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCGGGcta                                                                                       <  1:1565812/100‑3 (MQ=255)
             tctaCAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCga                                                                                      <  1:404232/97‑1 (MQ=255)
              taaCAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCgag                                                                                     <  1:783147/100‑1 (MQ=255)
               aaCAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCgagg                                                                                    <  2:535280/100‑1 (MQ=255)
               aaCAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCgagg                                                                                    <  2:782668/100‑1 (MQ=255)
                aCAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCgagga                                                                                   <  1:713864/100‑1 (MQ=255)
                 cAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCgaggag                                                                                  <  2:216760/100‑1 (MQ=255)
                       gACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACa                                                                            >  1:1060719/1‑100 (MQ=255)
                       gACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACa                                                                            <  2:1170242/100‑1 (MQ=255)
                        aCGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAg                                                                           <  1:2432356/100‑1 (MQ=255)
                        aCGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAg                                                                           <  2:2029376/100‑1 (MQ=255)
                         cGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGc                                                                          >  1:231725/1‑100 (MQ=255)
                         cGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGc                                                                          >  1:1445502/1‑100 (MQ=255)
                         cGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGc                                                                          >  1:770783/1‑100 (MQ=255)
                          gCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCa                                                                         <  1:1203102/100‑1 (MQ=255)
                           cAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCat                                                                        >  1:739117/1‑100 (MQ=255)
                           cAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGAAGACGAAGGGACGAGGAGATTAAAGAat                                                                        >  1:1955982/1‑100 (MQ=255)
                            agagCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCata                                                                       <  2:1119780/100‑1 (MQ=255)
                              aggacaAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATg                                                                     <  1:576305/97‑1 (MQ=255)
                               gCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGt                                                                    <  2:1616500/100‑1 (MQ=255)
                                cacaAATTATATTTCGAATGAAAGTAAGGTTGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGtt                                                                   <  1:875383/100‑1 (MQ=255)
                                cacaAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGtt                                                                   >  1:2072738/1‑100 (MQ=255)
                                 acaAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTc                                                                  <  2:1359850/100‑1 (MQ=255)
                                 acaAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTc                                                                  <  1:1076252/100‑1 (MQ=255)
                                  caAATTATATTTCGAATGAAAGTAAGGATGAACTTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCa                                                                 >  2:822109/1‑100 (MQ=255)
                                    aaTTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGt                                                               >  2:700682/1‑100 (MQ=255)
                                    aaTTATAGTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAAGGATTTATCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGt                                                               <  2:1206732/100‑1 (MQ=255)
                                     aTTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTa                                                              <  2:2456423/100‑1 (MQ=255)
                                         tatTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACggg                                                          <  1:1516803/100‑1 (MQ=255)
                                          atgtCGAATGAAAGGAAGGATGAAATGGATGAGGTGAATGAGTTAGCCCGGCGACGGCGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGc                                                         <  2:1528288/97‑1 (MQ=255)
                                          atTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGc                                                         <  1:427130/100‑1 (MQ=255)
                                           tttCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCGCGGCGACGACGCAGCCGGGGCGAGGAGATTACAGCATATGTTCAGTACggg                                                          >  2:1374614/1‑98 (MQ=255)
                                           tttCGAATGAAAGTAAGGATGAAATTGATGAGGTGAATGATTTAGCACGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCg                                                        >  2:1596305/1‑100 (MQ=255)
                                           tttCGAATGAAAGTAAGGATGAAATTGATGAGGTGAATGATCTAGCCCGGCGACGCCGCCGCCGGGCCGTGGAGATTACAGCATATGTTCAGTACGGTCg                                                        >  2:1596366/1‑100 (MQ=255)
                                             tcaaaTGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGTCGGGCCGAGGAGATTACAGCATATCTTCAGTACGGGCg                                                        <  2:1583069/95‑1 (MQ=255)
                                             tCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCgat                                                      <  1:1112521/100‑1 (MQ=255)
                                             tCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCgat                                                      <  1:2373622/100‑1 (MQ=255)
                                              cGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCGGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCgatg                                                     <  2:441381/100‑1 (MQ=255)
                                               gAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTCCGGGCgatg                                                     >  2:1845883/1‑99 (MQ=255)
                                                aaTGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCgatgat                                                   <  2:404515/100‑1 (MQ=255)
                                                aaTGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCgatgat                                                   >  2:2182190/1‑100 (MQ=255)
                                                aaTGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCgatgat                                                   >  1:2216286/1‑100 (MQ=255)
                                                aaTGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTAAAGCATATGTTCAGTACGGGCgatgat                                                   >  2:2198342/1‑100 (MQ=255)
                                                  tGAAAGTAAGGATGAACTTGATGATGTGAATGATCTCGCCCGACGACGACGCCGCCGGGACGAGGAGAATACAGCATATGTTCAGTACGGGcagtg                                                     >  2:2169327/1‑92 (MQ=255)
                                                  tGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGatat                                                 >  1:2398486/1‑100 (MQ=255)
                                                   gAAAGTAAGGATGAAATTGATGATGTGAAGGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGata                                                  <  2:183820/98‑1 (MQ=255)
                                                    aaaGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCg                                               <  2:1886792/100‑1 (MQ=255)
                                                           ggATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAg                                        >  1:2276020/1‑100 (MQ=255)
                                                              tGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCCTATGTTCAGTACGGGCGCTGATATCGTCCTGAGCg                                      >  2:2216131/1‑99 (MQ=255)
                                                               gAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTc                                    >  2:581923/1‑100 (MQ=255)
                                                               gAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTc                                    <  1:984448/100‑1 (MQ=255)
                                                                 aaTTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTg                                  <  2:1115258/100‑1 (MQ=255)
                                                                   ttGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGga                                <  2:2261925/100‑1 (MQ=255)
                                                                      tccgatctaaTGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGgagag                             <  2:2534547/92‑1 (MQ=255)
                                                                         atgTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAgc                          >  2:1993648/1‑100 (MQ=255)
                                                                         atgTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAgc                          <  2:1488023/100‑1 (MQ=255)
                                                                          tgtgAATTATTGAGCGCGGCGACGACGCCTCCTGGCCGATGAGATTACAGCATATGGTCAGTACGGGCGATGATATCGTCCTTAGCGTCTGGAGAGAgcg                         <  2:106393/100‑1 (MQ=255)
                                                                          tgtgAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAgcg                         >  1:1199067/1‑100 (MQ=255)
                                                                          tgtgAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAgcg                         >  1:437042/1‑100 (MQ=255)
                                                                           gtgAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAgcgc                        >  1:1644623/1‑100 (MQ=255)
                                                                           gtgAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAgcgc                        >  1:753113/1‑100 (MQ=255)
                                                                             gAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCgg                      <  1:707693/100‑1 (MQ=255)
                                                                             gAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCgg                      <  2:652793/100‑1 (MQ=255)
                                                                                 gATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTgaa                  >  1:2532008/1‑100 (MQ=255)
                                                                                 gATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTgaa                  <  2:2531993/100‑1 (MQ=255)
                                                                                 gATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTgaa                  <  1:787427/100‑1 (MQ=255)
                                                                                   tttAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTgaaga                >  1:1372181/1‑100 (MQ=255)
                                                                                   tttAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTgaaga                <  1:703924/100‑1 (MQ=255)
                                                                                     tAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAc              >  1:1246472/1‑100 (MQ=255)
                                                                                     cggCCCGGCGGCGTCGTCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAc              >  1:1932246/3‑100 (MQ=255)
                                                                                       gCCCGGCGGCGTCGTCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAcgc            >  1:76693/1‑100 (MQ=255)
                                                                                       gCCCGGCGGCGTCGTCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAcgc            >  1:303483/1‑100 (MQ=255)
                                                                                       gCCCGGCGGCGTCGTCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAcgc            >  1:89138/1‑100 (MQ=255)
                                                                                       gCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAcgc            <  1:26073/100‑1 (MQ=255)
                                                                                        cccGGCGGCGTCGTCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAcgcg           >  1:2193793/1‑100 (MQ=255)
                                                                                        cccGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAcgcg           >  2:726513/1‑100 (MQ=255)
                                                                                         ccGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCgg          >  2:2062559/1‑100 (MQ=255)
                                                                                         ccGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCgg          >  1:517280/1‑100 (MQ=255)
                                                                                         ccGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCgg          <  1:262273/100‑1 (MQ=255)
                                                                                           ggCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCGGaa        <  1:1110625/100‑1 (MQ=255)
                                                                                             cgacgaCGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCGGAATa      >  2:2317349/1‑100 (MQ=255)
                                                                                              gacgacGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCGGAATAg     >  2:1851662/1‑100 (MQ=255)
                                                                                                 gacgCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCGGAATAGccc  <  2:895149/100‑1 (MQ=255)
                                                                                                  |                                                                                                  
CCGTAACAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCC  >  NC_000913/860056‑860252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: