Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 860,157 C→T 16.3% intergenic (‑129/+17) ybiV ← / ← ybiW sugar phosphatase; fructose‑1‑P/ribose‑5‑P/glucose‑6‑P phosphatase/putative pyruvate formate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913860,1570CT16.3% 256.4 / 38.9 111intergenic (‑129/+17)ybiV/ybiWsugar phosphatase; fructose‑1‑P/ribose‑5‑P/glucose‑6‑P phosphatase/putative pyruvate formate lyase
Reads supporting (aligned to +/- strand):  ref base C (42/50);  new base T (6/12);  total (49/62)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.02e-01

Only 100 of 116 total aligned reads displayed.

CAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCG  >  NC_000913/860062‑860255
                                                                                               |                                                                                                  
cAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCgtcgcc                                                                                                <  2:579758/100‑1 (MQ=255)
cAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACgcagcc                                                                                                >  2:2489249/1‑100 (MQ=255)
   tgtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCggg                                                                                             <  2:1470053/100‑1 (MQ=255)
   tgtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCggg                                                                                             <  1:980689/100‑1 (MQ=255)
   tgtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCggg                                                                                             <  1:310285/100‑1 (MQ=255)
   tgtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCggg                                                                                             <  2:1019228/100‑1 (MQ=255)
   tgtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCggg                                                                                             >  1:1426597/1‑100 (MQ=255)
    gtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCgggn                                                                                            <  1:2483601/100‑2 (MQ=255)
    gtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCGGGc                                                                                            <  1:71025/100‑1 (MQ=255)
    gtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCGGGc                                                                                            <  2:233329/100‑1 (MQ=255)
    gtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCGGGc                                                                                            <  2:80001/100‑1 (MQ=255)
    gtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCGGGc                                                                                            <  1:110388/100‑1 (MQ=255)
    gtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGc                                                                                            <  2:2391333/100‑1 (MQ=255)
     taataaCAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCGGGct                                                                                           <  1:1920976/100‑2 (MQ=255)
     taataaCAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGcc                                                                                           >  1:92031/1‑100 (MQ=255)
      aataaCAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGGCGTCGTCGCCGGGcta                                                                                          <  1:1565812/100‑3 (MQ=255)
      aataaCAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCg                                                                                          >  1:1720997/1‑100 (MQ=255)
        taaCAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCgag                                                                                        <  1:783147/100‑1 (MQ=255)
         aaCAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCgagg                                                                                       <  2:535280/100‑1 (MQ=255)
         aaCAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCgagg                                                                                       <  2:782668/100‑1 (MQ=255)
          aCAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCgagga                                                                                      <  1:713864/100‑1 (MQ=255)
           cAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCgaggag                                                                                     <  2:216760/100‑1 (MQ=255)
             aTGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGAt                                                                                   <  1:440274/100‑1 (MQ=255)
                 gACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACa                                                                               >  1:1060719/1‑100 (MQ=255)
                  aCGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAg                                                                              <  2:2029376/100‑1 (MQ=255)
                   cGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGc                                                                             >  1:231725/1‑100 (MQ=255)
                   cGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGc                                                                             >  1:770783/1‑100 (MQ=255)
                   cGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGc                                                                             >  1:1445502/1‑100 (MQ=255)
                    gCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCa                                                                            <  1:1203102/100‑1 (MQ=255)
                     cAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCat                                                                           >  1:739117/1‑100 (MQ=255)
                     cAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGAAGACGAAGGGACGAGGAGATTAAAGAat                                                                           >  1:1955982/1‑100 (MQ=255)
                      agagCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCata                                                                          <  2:1119780/100‑1 (MQ=255)
                        aggacaAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATg                                                                        <  1:576305/97‑1 (MQ=255)
                         gCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGt                                                                       <  2:1616500/100‑1 (MQ=255)
                          cacaAATTATATTTCGAATGAAAGTAAGGTTGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGtt                                                                      <  1:875383/100‑1 (MQ=255)
                          cacaAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGtt                                                                      >  2:1774504/1‑100 (MQ=255)
                          cacaAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGtt                                                                      >  1:2072738/1‑100 (MQ=255)
                           acaAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTc                                                                     <  2:1359850/100‑1 (MQ=255)
                           acaAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTc                                                                     <  1:1076252/100‑1 (MQ=255)
                            caAATTATATTTCGAATGAAAGTAAGGATGAACTTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCa                                                                    >  2:822109/1‑100 (MQ=255)
                              aaTTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGt                                                                  >  2:700682/1‑100 (MQ=255)
                              aaTTATAGTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAAGGATTTATCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGt                                                                  <  2:1206732/100‑1 (MQ=255)
                               aTTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTa                                                                 <  2:2456423/100‑1 (MQ=255)
                                   tatTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACggg                                                             <  1:1516803/100‑1 (MQ=255)
                                    atTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGc                                                            <  1:427130/100‑1 (MQ=255)
                                     tttCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCGCGGCGACGACGCAGCCGGGGCGAGGAGATTACAGCATATGTTCAGTACggg                                                             >  2:1374614/1‑98 (MQ=255)
                                     tttCGAATGAAAGTAAGGATGAAATTGATGAGGTGAATGATTTAGCACGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCg                                                           >  2:1596305/1‑100 (MQ=255)
                                     tttCGAATGAAAGTAAGGATGAAATTGATGAGGTGAATGATCTAGCCCGGCGACGCCGCCGCCGGGCCGTGGAGATTACAGCATATGTTCAGTACGGTCg                                                           >  2:1596366/1‑100 (MQ=255)
                                       tcaaaTGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGTCGGGCCGAGGAGATTACAGCATATCTTCAGTACGGGCg                                                           <  2:1583069/95‑1 (MQ=255)
                                       tCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCgat                                                         <  1:2373622/100‑1 (MQ=255)
                                       tCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCgat                                                         <  1:1112521/100‑1 (MQ=255)
                                        cGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCGGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCgatg                                                        <  2:441381/100‑1 (MQ=255)
                                         gAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTCCGGGCgatg                                                        >  2:1845883/1‑99 (MQ=255)
                                          aaTGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCgatgat                                                      >  1:2216286/1‑100 (MQ=255)
                                          aaTGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCgatgat                                                      >  2:2182190/1‑100 (MQ=255)
                                          aaTGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCgatgat                                                      <  2:404515/100‑1 (MQ=255)
                                          aaTGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTAAAGCATATGTTCAGTACGGGCgatgat                                                      >  2:2198342/1‑100 (MQ=255)
                                            tGAAAGTAAGGATGAACTTGATGATGTGAATGATCTCGCCCGACGACGACGCCGCCGGGACGAGGAGAATACAGCATATGTTCAGTACGGGcagtg                                                        >  2:2169327/1‑92 (MQ=255)
                                            tGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGatat                                                    >  1:2398486/1‑100 (MQ=255)
                                              aaaGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCg                                                  <  2:1886792/100‑1 (MQ=255)
                                                     ggATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAg                                           >  1:2276020/1‑100 (MQ=255)
                                                        tGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCCTATGTTCAGTACGGGCGCTGATATCGTCCTGAGCg                                         >  2:2216131/1‑99 (MQ=255)
                                                         gAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTc                                       >  2:581923/1‑100 (MQ=255)
                                                         gAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTc                                       <  1:984448/100‑1 (MQ=255)
                                                           aaTTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTg                                     <  2:1115258/100‑1 (MQ=255)
                                                             ttGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGga                                   <  2:2261925/100‑1 (MQ=255)
                                                             ttGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGga                                   <  2:2276006/100‑1 (MQ=255)
                                                                tccgatctaaTGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGgagag                                <  2:2534547/92‑1 (MQ=255)
                                                                 tgatgTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGgagaga                               >  1:2216556/1‑100 (MQ=255)
                                                                   atgTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAgc                             >  2:1993648/1‑100 (MQ=255)
                                                                   atgTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAgc                             <  2:1488023/100‑1 (MQ=255)
                                                                    tgtgAATTATTGAGCGCGGCGACGACGCCTCCTGGCCGATGAGATTACAGCATATGGTCAGTACGGGCGATGATATCGTCCTTAGCGTCTGGAGAGAgcg                            <  2:106393/100‑1 (MQ=255)
                                                                    tgtgAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAgcg                            >  1:1199067/1‑100 (MQ=255)
                                                                    tgtgAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAgcg                            >  1:437042/1‑100 (MQ=255)
                                                                     gtgAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAgcgc                           >  1:1644623/1‑100 (MQ=255)
                                                                       gAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCgg                         <  2:652793/100‑1 (MQ=255)
                                                                       gAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCgg                         <  1:707693/100‑1 (MQ=255)
                                                                        aaTGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGagagagatcgga                        >  1:2534562/1‑94 (MQ=255)
                                                                           gATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTgaa                     <  2:2531993/100‑1 (MQ=255)
                                                                           gATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTgaa                     <  1:787427/100‑1 (MQ=255)
                                                                           gATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTgaa                     >  1:2532008/1‑100 (MQ=255)
                                                                            aTTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTgaag                    <  1:711222/100‑1 (MQ=255)
                                                                             tttAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTgaaga                   >  1:1372181/1‑100 (MQ=255)
                                                                             tttAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTgaaga                   <  1:703924/100‑1 (MQ=255)
                                                                               tAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAc                 >  1:1246472/1‑100 (MQ=255)
                                                                               cggCCCGGCGGCGTCGTCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAc                 >  1:1932246/3‑100 (MQ=255)
                                                                                 gCCCGGCGGCGTCGTCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAcgc               >  1:303483/1‑100 (MQ=255)
                                                                                 gCCCGGCGGCGTCGTCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAcgc               >  1:76693/1‑100 (MQ=255)
                                                                                 gCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAcgc               <  1:26073/100‑1 (MQ=255)
                                                                                  cccGGCGGCGTCGTCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAcgcg              >  1:866929/1‑100 (MQ=255)
                                                                                  cccGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAcgcg              >  2:726513/1‑100 (MQ=255)
                                                                                  cccGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGAGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAAcgcg              <  2:1425673/100‑1 (MQ=255)
                                                                                   ccGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCgg             >  2:2062559/1‑100 (MQ=255)
                                                                                   ccGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCgg             <  1:262273/100‑1 (MQ=255)
                                                                                   ccGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCgg             >  1:517280/1‑100 (MQ=255)
                                                                                     ggCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCGGaa           <  1:1110625/100‑1 (MQ=255)
                                                                                        gacgacGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCGGAATAg        >  2:1851662/1‑100 (MQ=255)
                                                                                           gacgCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCGGAATAGccc     <  2:895149/100‑1 (MQ=255)
                                                                                              gccgccGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCcgcg  <  2:1820954/100‑1 (MQ=255)
                                                                                              gccgccGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCcgcg  >  1:611920/1‑100 (MQ=255)
                                                                                               |                                                                                                  
CAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGTCTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCG  >  NC_000913/860062‑860255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: