Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	905,720	C→A	100%	L270M (CTG→ATG)	amiD →	1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase, Zn‑dependent; OM lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	905,720	0	C	A	100.0%	314.5 / ‑6.7	96	L270M (CTG→ATG)	amiD	1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase, Zn‑dependent; OM lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base A (51/43);  minor base G (0/1);  total (52/44)

GCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGC  >  NC_000913/905623‑905815                                                                                                  |                                                                                                ggagcCGCTCGTGATCATGCCATTCCAGATGCATTTCCGCCCGGCGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATg                                                                                               <  1:493262/98‑1 (MQ=255) gcagcGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATg                                                                                               <  1:437983/100‑1 (MQ=255)   agcGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGga                                                                                             >  2:2364483/1‑100 (MQ=255)     cggcgCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGgaga                                                                                           <  2:2001757/100‑1 (MQ=255)     cggcgCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGgaga                                                                                           >  2:48189/1‑100 (MQ=255)     cggcgCGTGATTATGGCATGCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGgag                                                                                            <  1:2544494/99‑1 (MQ=255)      ggcgCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGaa                                                                                          >  2:28608/1‑100 (MQ=255)       gcgcGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGaaa                                                                                         <  2:1294838/100‑1 (MQ=255)         gcgTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATa                                                                                       >  2:187434/1‑100 (MQ=255)           gTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACg                                                                                     >  2:1863804/1‑100 (MQ=255)            tGATTCTGGCATTGCAGATGCATTTCCGCACGACGTTATATCACGGCGACGCGGATGCAGAAAACAAGGAAATTGCCGAAGCATTGACGGAGAAATAcag                                                                                    >  2:199011/1‑98 (MQ=255)             gATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGCAACTCAGGCGATTGCCCACGCATTGATGGCGAACTACggg                                                                                   >  2:2147958/1‑100 (MQ=255)              aTTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGc                                                                                  >  1:1404513/1‑100 (MQ=255)              aTTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGc                                                                                  <  2:2477912/100‑1 (MQ=255)               ttATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCa                                                                                 <  2:271049/100‑1 (MQ=255)                tATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAg                                                                                <  1:2483072/100‑1 (MQ=255)                tATGGCAGTCCAGATGCAGTTCCGCCCGCCGTTCTATAATGGCTACGAGCGTGCAGAAACTCAGGCGATTGCCGAAGCATTGATGTAGAAATACgg                                                                                    <  2:903748/96‑1 (MQ=255)                 aTGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAgg                                                                               >  1:267344/1‑100 (MQ=255)                 aTGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAgg                                                                               >  2:1414941/1‑100 (MQ=255)                 aTGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAgg                                                                               >  1:1097183/1‑100 (MQ=255)                 aTGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAgg                                                                               <  1:2128791/100‑1 (MQ=255)                  tGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGa                                                                              <  1:553334/100‑1 (MQ=255)                  tGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGa                                                                              >  2:1174261/1‑100 (MQ=255)                   ggCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGAt                                                                             <  1:2122726/100‑1 (MQ=255)                    gCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGa                                                                              <  1:2284550/98‑1 (MQ=255)                    gCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGAtt                                                                            >  2:596850/1‑100 (MQ=255)                    gCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGAtt                                                                            <  2:1158382/100‑1 (MQ=255)                    gCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGAtt                                                                            <  1:31547/100‑1 (MQ=255)                      aTTCCAGAGGCATTTCCGCCCGACGGTATATAAGGGTGAAGCGGATGCAGAAACGCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAg                                                                          <  2:1126410/100‑1 (MQ=255)                         ccAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAgcgc                                                                       >  2:1886372/1‑100 (MQ=255)                         ccAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAgcgc                                                                       <  1:1556963/100‑1 (MQ=255)                          cAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGcgcg                                                                      <  1:2144459/100‑1 (MQ=255)                          cAGATGCATTTCCGCCCGACGTTATAGAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAgcgc                                                                       >  1:1557958/1‑99 (MQ=255)                            gacgCATTTCCGCCCTACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCGGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGc                                                                    <  2:23242/97‑1 (MQ=255)                              tGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAg                                                                  <  1:1721671/100‑1 (MQ=255)                               gCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGt                                                                 >  1:480615/1‑100 (MQ=255)                                cATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGtt                                                                >  2:823917/1‑100 (MQ=255)                                 aTTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGttt                                                               >  2:688772/1‑100 (MQ=255)                                 aTTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGttt                                                               >  2:841376/1‑100 (MQ=255)                                   ttCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTc                                                             >  1:1617791/1‑100 (MQ=255)                                     ccgccgGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCg                                                           <  2:2348046/100‑1 (MQ=255)                                     ccGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCg                                                           <  1:480716/100‑1 (MQ=255)                                      cGCCCGACGTTATATAACGGGGAAGCGGATGCAGAACCTCAGGCGATTGCCGCAGCCTTGATGGAGACATAAGGGCAGGATTAGCGCGGCCGTTTTCCGt                                                          <  2:2435055/100‑1 (MQ=255)                                       gCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTg                                                         <  1:2086605/100‑1 (MQ=255)                                       gCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTg                                                         >  2:319959/1‑100 (MQ=255)                                        cccGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTgg                                                        >  2:1774332/1‑100 (MQ=255)                                        cccGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTgg                                                        <  1:1448773/100‑1 (MQ=255)                                        cccGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTgg                                                        >  2:1134481/1‑100 (MQ=255)                                         ccGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGt                                                       >  2:948502/1‑100 (MQ=255)                                           gACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATAAGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTcg                                                     >  2:1137466/1‑100 (MQ=255)                                            aCGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTcgc                                                    >  2:2544550/1‑100 (MQ=255)                                              gTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGt                                                  <  2:1531273/100‑1 (MQ=255)                                               ttATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTa                                                 <  2:747464/100‑1 (MQ=255)                                                tatataACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAg                                                >  2:2204803/1‑100 (MQ=255)                                                   ataATGGCGAAGCGGATGCAGAAACTCAGGCGGTTGGCGAAGGAGTGATGGGGGAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAg                                                <  2:2191613/97‑1 (MQ=255)                                                   ataACGGCGGAGCCGATGTAGAAACTCAGGCGATTGCCGAAGGATTTGTGGCGTTACACGGGCGGGATGAGCGCGGCAGTTTTCCGTGGTCGCGTAGc                                               <  2:324647/98‑1 (MQ=255)                                                    taACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAg                                            >  2:2016946/1‑100 (MQ=255)                                                     aaCGGCGAAGCGGATGCAGAGACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAgg                                           >  2:1565351/1‑100 (MQ=255)                                                     aaCGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAgg                                           >  1:15329/1‑100 (MQ=255)                                                      aCGGCGACGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAggc                                          >  2:2191986/1‑100 (MQ=255)                                                       cGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAggcg                                         >  1:2486602/1‑100 (MQ=255)                                                        ggCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAggcgg                                        <  1:2197228/100‑1 (MQ=255)                                                         gCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAggcggc                                       >  1:2114941/1‑100 (MQ=255)                                                          cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCACGTAGCCAGGCGGCa                                      >  1:2521350/1‑100 (MQ=255)                                                              gCGTATGCAGAAACACAGGCGATTGCAGAAGCATTGAGGGAGAAAGACGGCCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGttc                                  <  2:2157328/100‑2 (MQ=255)                                                              gCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGttt                                  <  2:878703/100‑1 (MQ=255)                                                               cGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGtttt                                 <  1:788624/100‑1 (MQ=255)                                                                ggATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtc                                >  1:17307/1‑100 (MQ=255)                                                                ggATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtc                                <  1:2022993/100‑1 (MQ=255)                                                                ggATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtc                                >  1:921123/1‑100 (MQ=255)                                                                  aTGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtctc                              <  1:1310864/100‑1 (MQ=255)                                                                  aTGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtctc                              <  2:345270/100‑1 (MQ=255)                                                                   tGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCg                             >  2:1414516/1‑100 (MQ=255)                                                                   tGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCg                             <  1:1934516/100‑1 (MQ=255)                                                                       gAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATAc                         >  1:755218/1‑100 (MQ=255)                                                                             cAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTc                     <  2:1742961/98‑1 (MQ=255)                                                                             cAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCAt                   >  2:777963/1‑100 (MQ=255)                                                                             cAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCAt                   <  1:376649/100‑1 (MQ=255)                                                                              aGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATc                  >  2:779184/1‑100 (MQ=255)                                                                                 cGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAg               >  1:1228796/1‑100 (MQ=255)                                                                                 cGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAg               <  1:2147214/100‑1 (MQ=255)                                                                                  gATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAgg              <  2:582989/100‑1 (MQ=255)                                                                                   aTTGCCGAAGCATTGATGGGGAAATAGGGGCAGGATTAGCGCTGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAggg             >  2:2435896/1‑100 (MQ=255)                                                                                   aTTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAggg             >  1:1488908/1‑100 (MQ=255)                                                                                      gCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtga          >  1:2346806/1‑100 (MQ=255)                                                                                       ccGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtgat         >  1:1657530/1‑100 (MQ=255)                                                                                       ccGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtgat         >  1:1070936/1‑100 (MQ=255)                                                                                        cGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtgatg        >  1:2163439/1‑100 (MQ=255)                                                                                         gAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtgatga       >  1:2328518/1‑100 (MQ=255)                                                                                         gAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtgatga       <  1:2559172/100‑1 (MQ=255)                                                                                          aaGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGAc      >  2:469864/1‑100 (MQ=255)                                                                                           aGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGAcc     <  1:1174269/100‑1 (MQ=255)                                                                                             cATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCgg   >  1:1389410/1‑100 (MQ=255)                                                                                             cATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCgg   >  1:1020176/1‑100 (MQ=255)                                                                                              aTTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGc  >  1:502202/1‑100 (MQ=255)                                                                                               ttGATGGAGAAATACGGGCAGGATGAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCgg   <  2:154696/98‑1 (MQ=255)                                                                                                  |                                                                                                GCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGC  >  NC_000913/905623‑905815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑