| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 975,083 | T→A | 100% | D255E (GAT→GAA) | mukF → | chromosome condensin MukBEF, kleisin‑like subunit, binds calcium |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 975,083 | 0 | T | A | 98.8% | 280.2 / ‑5.0 | 82 | D255E (GAT→GAA) | mukF | chromosome condensin MukBEF, kleisin‑like subunit, binds calcium |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base A (32/49); total (33/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGT > NC_000913/974989‑975176 | cGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCa < 2:1888013/100‑1 (MQ=255)cGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCa > 1:1752405/1‑100 (MQ=255) gCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCaga < 1:787590/100‑1 (MQ=255) cAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCagag < 2:985658/100‑1 (MQ=255) cAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCagag < 2:767220/100‑1 (MQ=255) cAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCagag < 1:93759/100‑1 (MQ=255) cAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCagag < 1:579695/100‑1 (MQ=255) ggCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCa > 1:77918/1‑100 (MQ=255) ggCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCa < 2:1866983/100‑1 (MQ=255) gCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCaa > 1:2333896/1‑100 (MQ=255) gACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAAc < 1:703535/100‑1 (MQ=255) cAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTc < 1:840538/100‑1 (MQ=255) naaTTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCg > 1:594758/2‑100 (MQ=255) aaaTTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCg < 2:1702966/100‑1 (MQ=255) aaaTTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCg < 1:1172283/100‑1 (MQ=255) aaaTTGCAGGCTAATCTGTTGCGCAGTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCg < 2:378761/100‑1 (MQ=255) aTTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGAt > 1:214334/1‑100 (MQ=255) ttGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATc < 1:864425/100‑1 (MQ=255) ttGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATc < 1:1722110/100‑1 (MQ=255) tGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCg < 1:2237975/100‑1 (MQ=255) tGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCg > 1:2002987/1‑100 (MQ=255) tGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTCTTCGAACTGCAGAGCAAACTCGATCg < 1:1555088/100‑1 (MQ=255) gCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGt > 2:2483385/1‑100 (MQ=255) gCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGt < 1:855058/100‑1 (MQ=255) aGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGtat < 1:2412671/100‑1 (MQ=255) cTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGtatta < 2:601680/99‑1 (MQ=255) tCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGt > 2:2326044/1‑100 (MQ=255) gTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTgg > 1:287992/1‑100 (MQ=255) tGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTgggg < 1:2464277/100‑1 (MQ=255) tGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTgggg > 2:1297000/1‑100 (MQ=255) gcgcATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGc < 1:156733/100‑1 (MQ=255) gcgcATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGc < 1:2465225/100‑1 (MQ=255) cgcATTCAGGATGCGTCGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGcc < 1:2520623/100‑1 (MQ=255) cgcATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGcc < 2:2372926/100‑1 (MQ=255) cgcATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGcc > 1:1518346/1‑100 (MQ=255) cATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAg > 1:1827698/1‑100 (MQ=255) aTTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGc < 2:1202749/100‑1 (MQ=255) aTTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGc < 2:1288316/100‑1 (MQ=255) cAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAAt < 1:271917/100‑1 (MQ=255) ggATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATcc > 1:1718047/1‑100 (MQ=255) ggATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATcc > 1:148501/1‑100 (MQ=255) gATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCa > 1:2149057/1‑100 (MQ=255) aTGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAACCCAt < 1:1894171/100‑1 (MQ=255) tGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATc < 1:1445326/100‑1 (MQ=255) gCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCg < 1:1532203/100‑1 (MQ=255) cgacgaTGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGa < 1:154309/100‑1 (MQ=255) cgacgaTGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGa > 2:2365434/1‑100 (MQ=255) cgacgaTGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGa > 2:536754/1‑100 (MQ=255) acgaTGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACt > 2:755417/1‑100 (MQ=255) acgaTGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACt < 1:2554072/100‑1 (MQ=255) cgaTGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACtt < 1:755424/100‑1 (MQ=255) tGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTtgt > 2:1263226/1‑99 (MQ=255) gACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTtgtg < 2:2208804/99‑1 (MQ=255) gACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTgg > 2:1914997/1‑100 (MQ=255) aCCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGa > 2:1087451/1‑100 (MQ=255) cccATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGAt > 2:2533342/1‑100 (MQ=255) aTGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTgg < 2:1901282/100‑1 (MQ=255) tGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGc > 1:1606067/1‑100 (MQ=255) cGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTAc > 1:132344/1‑100 (MQ=255) cGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTAc > 2:2363614/1‑100 (MQ=255) gATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACg > 2:970029/1‑100 (MQ=255) tCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGAc < 2:2128600/100‑1 (MQ=255) cTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGAcc < 1:114423/100‑1 (MQ=255) gCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGc > 1:2479877/1‑100 (MQ=255) cATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGcc < 1:2119176/100‑1 (MQ=255) aTTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCa < 2:1905503/100‑1 (MQ=255) ttCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACg < 2:2363441/100‑1 (MQ=255) ttCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACg < 2:208737/100‑1 (MQ=255) ttCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACg < 2:587648/100‑1 (MQ=255) tcgtcgATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCAc < 2:2435608/98‑1 (MQ=255) gtcgATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTac > 1:1525404/1‑100 (MQ=255) cgATCGTCTGGTGTTCGAACTGTAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTacac < 1:2090204/100‑1 (MQ=255) gATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTAcaca > 1:1168346/1‑100 (MQ=255) gATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTAcaca > 2:2491546/1‑100 (MQ=255) cGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAAt < 1:970037/100‑1 (MQ=255) gTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAAtt > 2:2037424/1‑100 (MQ=255) tCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAAttt > 1:1537823/1‑100 (MQ=255) tGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTAt < 1:163378/100‑1 (MQ=255) gtgtTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTc > 2:663817/1‑100 (MQ=255) tgtTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCg < 2:2507615/100‑1 (MQ=255) tgtTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCg < 1:1547841/100‑1 (MQ=255) gtTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGt < 1:1315802/100‑1 (MQ=255) | CGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGT > NC_000913/974989‑975176 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |