Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,113,546 T→A 15.3% intergenic (+140/‑33) opgH → / → yceK OPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist/outer membrane integrity lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,113,5460TA15.3% 359.4 / 46.7 139intergenic (+140/‑33)opgH/yceKOPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist/outer membrane integrity lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (43/74);  new base A (7/14);  total (51/88)
Fisher's exact test for biased strand distribution p-value = 8.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 141 total aligned reads displayed.

CGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCA  >  NC_000913/1113448‑1113645
                                                                                                  |                                                                                                   
cGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTa                                                                                                    <  1:135161/100‑1 (MQ=255)
 gTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTAt                                                                                                   >  2:744737/1‑100 (MQ=255)
 gTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTAt                                                                                                   <  1:821041/100‑1 (MQ=255)
    ccTGCAATTTATTGATTCTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGcc                                                                                                >  1:1707300/1‑100 (MQ=255)
     cTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCg                                                                                               >  1:1866918/1‑100 (MQ=255)
      tGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCgg                                                                                              <  1:1054518/100‑1 (MQ=255)
       gCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGt                                                                                             <  1:123809/100‑1 (MQ=255)
        cAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTa                                                                                            >  2:334666/1‑100 (MQ=255)
          aTTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAtt                                                                                          <  2:1975597/100‑1 (MQ=255)
           tttATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAttt                                                                                         <  1:1742663/100‑1 (MQ=25)
            ttATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTAtttt                                                                                        <  2:1034250/100‑1 (MQ=255)
            ttATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAtttt                                                                                        <  1:2091055/100‑1 (MQ=37)
             tATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCGCGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAtttt                                                                                        <  1:1000556/99‑1 (MQ=37)
             tATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAttttt                                                                                       <  2:454433/100‑1 (MQ=37)
              aTTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTAtttttt                                                                                      <  1:1743310/100‑1 (MQ=255)
              aTTGATTTTGCAAGACTTTGTGGGTCAGATAAGGCGTTCACGCCGCATCCGGCAAAAAAAACTAGCCAATAAAAATACCGGCATAACGCCGGTAtttt                                                                                        <  2:1298064/98‑1 (MQ=25)
               ttGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAtttttat                                                                                     <  2:1534909/100‑3 (MQ=37)
                tGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTa                                                                                    <  1:1721219/100‑1 (MQ=255)
                tGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTa                                                                                    <  1:2326731/100‑1 (MQ=255)
                tGATTTTGCAAGACTTTGTGGGGCAGATACGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAttt                                                                                         <  2:779750/95‑1 (MQ=25)
                 gATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCATGCCGCATCCGGCAAAAACATCGAGCCAATAAAAATACCGGCATAACGCCGGTAtttttatt                                                                                    <  2:586622/99‑4 (MQ=37)
                 gATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTAc                                                                                   <  2:1710119/100‑1 (MQ=255)
                 gATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTAc                                                                                   <  2:1215172/100‑1 (MQ=255)
                 gATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTAc                                                                                   <  1:429435/100‑1 (MQ=255)
                 gATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAtttttattg                                                                                   <  1:1576332/100‑5 (MQ=25)
                  aTTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACg                                                                                  >  2:454524/1‑100 (MQ=255)
                  aTTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAtttttattgg                                                                                  <  2:419542/100‑6 (MQ=25)
                   ttttGCAAGACTTTGTGTGTCGGATAAGGCGTAAACGCCGCATCCGGCAAAAATAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTa                                                                                    <  2:1714355/97‑1 (MQ=38)
                       gCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAaga                                                                             >  1:1940245/1‑100 (MQ=255)
                        cAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAagag                                                                            >  2:1157433/1‑100 (MQ=255)
                        cAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAagag                                                                            <  1:1761418/100‑1 (MQ=255)
                         aaGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAgg                                                                           <  1:1774684/100‑1 (MQ=255)
                          aGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGt                                                                          >  1:634152/1‑100 (MQ=255)
                           gACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTa                                                                         <  1:1161587/100‑1 (MQ=255)
                             cTTTGGGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAACAAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATc                                                                       <  2:1591481/100‑1 (MQ=255)
                               ttGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCaa                                                                     <  1:1445908/100‑1 (MQ=255)
                                    ggTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCg                                                                <  2:1944559/100‑1 (MQ=255)
                                    ggTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCg                                                                >  2:1715614/1‑100 (MQ=255)
                                     gTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGt                                                               >  2:1131148/1‑100 (MQ=255)
                                       cGGATAAGGCGTTCACGCCGCATCCGGAAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGtt                                                              >  2:1073517/1‑99 (MQ=255)
                                        ggATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTa                                                            <  1:887534/100‑1 (MQ=255)
                                             aGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATtgt                                                       >  1:1565431/1‑100 (MQ=255)
                                               gCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTgtgg                                                     <  2:344966/100‑1 (MQ=255)
                                               gCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTgtgg                                                     >  1:2457035/1‑100 (MQ=255)
                                               gCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTgtgg                                                     <  1:1501639/100‑1 (MQ=255)
                                                 gTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTgtggtg                                                   <  2:1834630/100‑1 (MQ=255)
                                                 gTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTgtggtg                                                   >  1:538512/1‑100 (MQ=255)
                                                 gTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTgtggtg                                                   <  1:744744/100‑1 (MQ=255)
                                                  ttCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGa                                                  >  2:92605/1‑100 (MQ=255)
                                                   tCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAg                                                 <  1:214277/100‑1 (MQ=255)
                                                   tCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAg                                                 <  2:1940231/100‑1 (MQ=255)
                                                   tCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGGTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTgtggtg                                                   >  2:485349/1‑98 (MQ=255)
                                                     ncgccgcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGca                                               >  2:1804816/2‑100 (MQ=255)
                                                     aCGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAg                                                 <  2:717531/98‑1 (MQ=255)
                                                      cgccgcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGcat                                              <  1:2379195/100‑1 (MQ=255)
                                                      cgccgcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGcat                                              >  1:1226852/1‑100 (MQ=255)
                                                       gccgcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTCAATTGTGGTGAGcatc                                             >  2:1496320/1‑100 (MQ=255)
                                                         cgcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGcatcat                                           <  2:734988/100‑1 (MQ=255)
                                                          gcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATg                                          <  1:577891/100‑1 (MQ=255)
                                                               cGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGAc                                     <  2:1542832/100‑1 (MQ=255)
                                                               cGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGAc                                     >  1:1128569/1‑100 (MQ=255)
                                                                ggCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGAcc                                    <  1:556203/100‑1 (MQ=255)
                                                                ggCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGAcc                                    <  1:384840/100‑1 (MQ=255)
                                                                ggCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGAcc                                    >  1:193252/1‑100 (MQ=255)
                                                                 gCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCt                                   >  2:1047019/1‑100 (MQ=255)
                                                                  cAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCtt                                  <  2:2370030/100‑1 (MQ=255)
                                                                  cAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCtt                                  <  1:2358400/100‑1 (MQ=255)
                                                                   aaaaaCAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTg                                 <  1:1502049/100‑1 (MQ=255)
                                                                    aaaaCAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGAATCAAATGCGTTTAATTGTTGTGAGCATCATGGTGACCTTGc                                >  2:1949537/1‑100 (MQ=255)
                                                                      aacaacGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTg                              <  1:306999/100‑1 (MQ=255)
                                                                          acGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTg                          <  2:143558/100‑1 (MQ=255)
                                                                          acGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTg                          <  2:1296986/100‑1 (MQ=255)
                                                                          acGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTg                          <  1:1325333/100‑1 (MQ=255)
                                                                           cGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTgg                         >  1:2098373/1‑100 (MQ=255)
                                                                            gggCCAATAAAAATTCCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGc                        <  1:2376557/98‑1 (MQ=255)
                                                                            gAGCCAATAAAAATACCTGCGTTATGCCGGTATTTGTTTACGAAAGAGGGATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGc                        <  2:1191193/100‑1 (MQ=255)
                                                                            gAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGc                        <  1:288631/100‑1 (MQ=255)
                                                                             ttcgtaaaaaaaTACCGGCATAACGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCt                       >  1:2338259/8‑100 (MQ=37)
                                                                              gCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCtg                      <  1:1399845/100‑1 (MQ=255)
                                                                               cgtaaaaaaaTACCGGCATAACGCCGGTATTTTTTTACGAAAGAGGTACCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCtgt                     >  1:1700074/6‑100 (MQ=37)
                                                                                gtaaaaaaaTACCGGCATAACGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCtgtg                    >  1:1862323/5‑100 (MQ=37)
                                                                                 aaTAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTgg                   >  2:93564/1‑100 (MQ=255)
                                                                                 aaTAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGAATCATGGTGACCTTGCTGAGTGGAtgtg                    >  2:552125/1‑99 (MQ=255)
                                                                                  aTAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgc                  <  2:2409082/100‑1 (MQ=255)
                                                                                  aTAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgc                  <  2:2032298/100‑1 (MQ=255)
                                                                                  aTAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgc                  <  2:337725/100‑1 (MQ=255)
                                                                                  aTAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgc                  <  1:413633/100‑1 (MQ=255)
                                                                                  aTAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgc                  <  1:1058107/100‑1 (MQ=255)
                                                                                   tAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgca                 >  1:1853822/1‑100 (MQ=255)
                                                                                   aaaaaaTACCGGCATAACGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgca                 >  1:2521308/2‑100 (MQ=38)
                                                                                     aaaaTACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgcagc               >  1:973363/1‑100 (MQ=255)
                                                                                     aaaaTACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgcagc               <  1:649944/100‑1 (MQ=255)
                                                                                       aaTACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCat             <  1:2126844/100‑1 (MQ=255)
                                                                                          aCCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCattat          <  1:931598/100‑1 (MQ=255)
                                                                                           ccGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCattat          >  2:1243049/1‑99 (MQ=255)
                                                                                            cGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCattatt         <  2:1574507/99‑1 (MQ=255)
                                                                                            cGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCAttatta        >  1:2272042/1‑100 (MQ=255)
                                                                                             ggCGTTATGCCGGTATTTTTTTACTTATTTGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGTCAGCATTATTAg       >  2:1358229/1‑100 (MQ=255)
                                                                                                 ttATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGc   <  2:634145/100‑1 (MQ=255)
                                                                                                  tATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCa  >  2:2545406/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
CGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCA  >  NC_000913/1113448‑1113645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: