Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,113,548 T→C 15.1% intergenic (+142/‑31) opgH → / → yceK OPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist/outer membrane integrity lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,113,5480TC15.1% 329.7 / 46.7 139intergenic (+142/‑31)opgH/yceKOPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist/outer membrane integrity lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (43/75);  new base C (7/14);  total (50/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01

Only 100 of 141 total aligned reads displayed.

GTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCAC  >  NC_000913/1113449‑1113646
                                                                                                   |                                                                                                  
gTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTAt                                                                                                    >  2:744737/1‑100 (MQ=255)
gTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTAt                                                                                                    <  1:821041/100‑1 (MQ=255)
    cTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCg                                                                                                >  1:1866918/1‑100 (MQ=255)
     tGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCgg                                                                                               <  1:1054518/100‑1 (MQ=255)
     tGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCgg                                                                                               <  1:1870891/100‑1 (MQ=255)
      gCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGt                                                                                              <  1:123809/100‑1 (MQ=255)
       cAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTa                                                                                             >  2:334666/1‑100 (MQ=255)
         aTTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAtt                                                                                           <  2:1975597/100‑1 (MQ=255)
          tttATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAttt                                                                                          <  1:1742663/100‑1 (MQ=25)
           ttATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAtttt                                                                                         <  1:2091055/100‑1 (MQ=37)
            tATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCGCGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAtttt                                                                                         <  1:1000556/99‑1 (MQ=37)
            tATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAttttt                                                                                        <  2:454433/100‑1 (MQ=37)
             aTTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTAtttttt                                                                                       >  1:1990421/1‑100 (MQ=255)
             aTTGATTTTGCAAGACTTTGTGGGTCAGATAAGGCGTTCACGCCGCATCCGGCAAAAAAAACTAGCCAATAAAAATACCGGCATAACGCCGGTAtttt                                                                                         <  2:1298064/98‑1 (MQ=25)
              ttGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAtttttat                                                                                      <  2:1534909/100‑3 (MQ=37)
              ttGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAttttt                                                                                        <  1:1537361/98‑1 (MQ=37)
               tGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTa                                                                                     <  1:1721219/100‑1 (MQ=255)
               tGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTa                                                                                     <  1:2326731/100‑1 (MQ=255)
               tGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTa                                                                                     <  1:401933/100‑1 (MQ=255)
               tGATTTTGCAAGACTTTGTGGGGCAGATACGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAttt                                                                                          <  2:779750/95‑1 (MQ=25)
               tGATCTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTCTTTTTTTa                                                                                     >  2:1115442/1‑100 (MQ=255)
                gATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCATGCCGCATCCGGCAAAAACATCGAGCCAATAAAAATACCGGCATAACGCCGGTAtttttatt                                                                                     <  2:586622/99‑4 (MQ=37)
                gATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTAc                                                                                    <  2:1215172/100‑1 (MQ=255)
                gATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTAc                                                                                    >  1:2314726/1‑100 (MQ=255)
                gATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTAc                                                                                    <  1:429435/100‑1 (MQ=255)
                gATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTAc                                                                                    <  2:1710119/100‑1 (MQ=255)
                gATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAtttttattg                                                                                    <  1:2421893/100‑5 (MQ=25)
                 aTTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACg                                                                                   >  2:454524/1‑100 (MQ=255)
                 aTTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAtttttattgg                                                                                   <  2:419542/100‑6 (MQ=25)
                  ttttGCAAGACTTTGTGTGTCGGATAAGGCGTAAACGCCGCATCCGGCAAAAATAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTa                                                                                     <  2:1714355/97‑1 (MQ=38)
                     tGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAag                                                                               <  1:1400163/100‑1 (MQ=255)
                       cAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAagag                                                                             <  1:1761418/100‑1 (MQ=255)
                       cAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAagag                                                                             >  2:1157433/1‑100 (MQ=255)
                          gACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTa                                                                          <  1:1161587/100‑1 (MQ=255)
                            cTTTGGGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAACAAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATc                                                                        <  2:1591481/100‑1 (MQ=255)
                             tttGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCa                                                                       <  1:91900/100‑1 (MQ=255)
                              ttGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCaa                                                                      <  1:1445908/100‑1 (MQ=255)
                                   ggTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCg                                                                 >  2:1715614/1‑100 (MQ=255)
                                   ggTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCg                                                                 <  2:1944559/100‑1 (MQ=255)
                                    gTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGt                                                                >  2:1131148/1‑100 (MQ=255)
                                     tCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGtt                                                               <  1:1726318/100‑1 (MQ=255)
                                      cGGATAAGGCGTTCACGCCGCATCCGGAAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGtt                                                               >  2:1073517/1‑99 (MQ=255)
                                       ggATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTa                                                             <  1:887534/100‑1 (MQ=255)
                                           aaGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATtg                                                         <  1:293838/100‑1 (MQ=255)
                                            aGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATtgt                                                        >  1:1565431/1‑100 (MQ=255)
                                              gCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTgtgg                                                      <  1:1851150/100‑1 (MQ=255)
                                              gCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTgtgg                                                      <  2:344966/100‑1 (MQ=255)
                                                gTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTgtggtg                                                    <  2:1834630/100‑1 (MQ=255)
                                                 ttCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGa                                                   >  2:92605/1‑100 (MQ=255)
                                                  tCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAg                                                  <  1:214277/100‑1 (MQ=255)
                                                  tCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAg                                                  <  2:1940231/100‑1 (MQ=255)
                                                  tCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAg                                                  >  1:1636492/1‑100 (MQ=255)
                                                  tCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGGTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTgtggtg                                                    >  2:485349/1‑98 (MQ=255)
                                                    ncgccgcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGca                                                >  2:1804816/2‑100 (MQ=255)
                                                    aCGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAg                                                  <  2:717531/98‑1 (MQ=255)
                                                     cgccgcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGcat                                               <  1:2379195/100‑1 (MQ=255)
                                                     cgccgcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGcat                                               >  1:1226852/1‑100 (MQ=255)
                                                     cgccgcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTGAATTGTGGTGAGcat                                               >  1:991163/1‑100 (MQ=255)
                                                      gccgcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTCAATTGTGGTGAGcatc                                              >  2:1496320/1‑100 (MQ=255)
                                                        cgcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGcatcat                                            <  2:734988/100‑1 (MQ=255)
                                                         gcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATg                                           <  1:577891/100‑1 (MQ=255)
                                                             ccGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGa                                       <  1:2361614/100‑1 (MQ=255)
                                                              cGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGAc                                      >  1:1128569/1‑100 (MQ=255)
                                                              cGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGAc                                      <  2:1542832/100‑1 (MQ=255)
                                                               ggCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGAcc                                     <  1:556203/100‑1 (MQ=255)
                                                               ggCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGAcc                                     >  1:193252/1‑100 (MQ=255)
                                                               ggCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGAcc                                     <  1:384840/100‑1 (MQ=255)
                                                                gCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCt                                    >  2:1047019/1‑100 (MQ=255)
                                                                 cAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCtt                                   <  2:2370030/100‑1 (MQ=255)
                                                                 cAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCtt                                   <  1:2358400/100‑1 (MQ=255)
                                                                  aaaaaCAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTg                                  <  1:1502049/100‑1 (MQ=255)
                                                                   aaaaCAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGAATCAAATGCGTTTAATTGTTGTGAGCATCATGGTGACCTTGc                                 >  2:1949537/1‑100 (MQ=255)
                                                                    aaaCAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCt                                <  1:587277/100‑1 (MQ=255)
                                                                         acGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTg                           <  2:143558/100‑1 (MQ=255)
                                                                         acGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTg                           <  1:1325333/100‑1 (MQ=255)
                                                                         acGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTg                           <  2:1296986/100‑1 (MQ=255)
                                                                           gggCCAATAAAAATTCCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGc                         <  1:2376557/98‑1 (MQ=255)
                                                                           gAGCCAATAAAAATACCTGCGTTATGCCGGTATTTGTTTACGAAAGAGGGATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGc                         <  2:1191193/100‑1 (MQ=255)
                                                                           gAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGc                         <  1:288631/100‑1 (MQ=255)
                                                                              cgtaaaaaaaTACCGGCATAACGCCGGTATTTTTTTACGAAAGAGGTACCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCtgt                      >  1:1700074/6‑100 (MQ=37)
                                                                                aaTAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTgg                    >  2:93564/1‑100 (MQ=255)
                                                                                aaTAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGAATCATGGTGACCTTGCTGAGTGGAtgtg                     >  2:552125/1‑99 (MQ=255)
                                                                                 aaaaaaaTACCGGCATAACGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgc                   >  1:438604/3‑100 (MQ=38)
                                                                                 aTAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgc                   <  1:1058107/100‑1 (MQ=255)
                                                                                 aTAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgc                   <  2:2032298/100‑1 (MQ=255)
                                                                                 aTAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgc                   <  2:2409082/100‑1 (MQ=255)
                                                                                 aTAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgc                   <  2:337725/100‑1 (MQ=255)
                                                                                  tAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgca                  >  1:1853822/1‑100 (MQ=255)
                                                                                  aaaaaaTACCGGCATAACGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgca                  >  1:2521308/2‑100 (MQ=38)
                                                                                    aaaaTACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgcagc                >  1:973363/1‑100 (MQ=255)
                                                                                    aaaaTACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgcagc                <  1:649944/100‑1 (MQ=255)
                                                                                      aaTACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCat              <  1:2126844/100‑1 (MQ=255)
                                                                                      aaTACCGGCATAACGCCGGTATTTTTTTACGAAAGAGGTATCAAATGAGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgcagca               >  1:2137293/1‑99 (MQ=38)
                                                                                          ccGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCattatt          >  1:678272/1‑100 (MQ=255)
                                                                                          ccGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCattat           >  2:1243049/1‑99 (MQ=255)
                                                                                           cGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCattatt          <  2:1574507/99‑1 (MQ=255)
                                                                                            ggCGTTATGCCGGTATTTTTTTACTTATTTGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGTCAGCATTATTAg        >  2:1358229/1‑100 (MQ=255)
                                                                                            ggCGTTATGCCGGTATTTTGTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAg        <  1:1750716/100‑1 (MQ=255)
                                                                                                ttATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGc    <  2:634145/100‑1 (MQ=255)
                                                                                                 tATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCa   >  2:2545406/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
GTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCAC  >  NC_000913/1113449‑1113646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: