Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,129,394 G→C 15.2% intergenic (+20/+20) murJ → / ← flgN putative lipid II flippase/export chaperone for FlgK and FlgL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,129,3940GC15.2% 250.8 / 35.8 92intergenic (+20/+20)murJ/flgNputative lipid II flippase/export chaperone for FlgK and FlgL
Reads supporting (aligned to +/- strand):  ref base G (41/37);  new base C (3/11);  total (44/48)
Fisher's exact test for biased strand distribution p-value = 4.23e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

Only 100 of 101 total aligned reads displayed.

GGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCG  >  NC_000913/1129296‑1129493
                                                                                                  |                                                                                                   
ggCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCACAGTTAAAGAATTTGCCCGCCGGACGGAGTAACAATGCATTCCGGCCtgcagt                                                                                                    >  2:307270/1‑100 (MQ=255)
  cGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagtgc                                                                                                  >  1:45248/1‑100 (MQ=255)
   ggggATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagtgca                                                                                                 <  1:1764795/100‑1 (MQ=255)
    gggATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagtgcag                                                                                                <  1:1211590/100‑1 (MQ=255)
       aTTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGcc                                                                                             <  1:939947/100‑1 (MQ=255)
        ttGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCg                                                                                            >  1:2424259/1‑100 (MQ=255)
        ttGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCg                                                                                            >  2:1477167/1‑100 (MQ=255)
        ttGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCg                                                                                            <  2:396028/100‑1 (MQ=255)
        ttGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCg                                                                                            <  1:1758970/100‑1 (MQ=255)
         tGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGCCGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCg                                                                                            >  1:352351/1‑99 (MQ=255)
         tGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCgg                                                                                           >  1:351182/1‑100 (MQ=255)
         tGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCgg                                                                                           <  1:1630091/100‑1 (MQ=255)
         tGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCgg                                                                                           <  1:2093682/100‑1 (MQ=255)
         tGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCgg                                                                                           <  1:1433679/100‑1 (MQ=255)
         tGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCgg                                                                                           <  1:1335717/100‑1 (MQ=255)
          gCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCTGga                                                                                          >  2:296875/1‑100 (MQ=255)
          gCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGga                                                                                          <  1:1998682/100‑1 (MQ=255)
           cggcgTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCGgaa                                                                                         <  2:1554373/98‑2 (MQ=255)
            cgcgTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGTCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCGgaat                                                                                        <  1:541650/100‑3 (MQ=255)
            cgcgTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGgaga                                                                                        <  1:974104/100‑1 (MQ=255)
               gTACTTCGCTGCACTGGCGGTAGTGGCCTTCAATTTTAAAGAATTTGCCCGCCGGACGGTGTAACAATTCATTCCGGCCTGCACTGCAGGCCGgaat                                                                                        <  2:1125710/97‑3 (MQ=38)
                tACTTCGCTGCACTGGCGGTACTGGGCTTCAAATTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCGgaat                                                                                        <  2:687173/96‑3 (MQ=255)
                 aCTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATc                                                                                   >  2:292851/1‑100 (MQ=255)
                 aCTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCGgaatgcatt                                                                                   <  1:334069/100‑8 (MQ=38)
                 aCTTCGCGGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATc                                                                                   >  1:1339550/1‑100 (MQ=255)
                  cTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCt                                                                                  >  1:355106/1‑100 (MQ=255)
                    tCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTc                                                                                >  1:1412248/1‑100 (MQ=255)
                    tCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTc                                                                                >  2:100408/1‑100 (MQ=255)
                     cGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCa                                                                               <  1:773834/100‑1 (MQ=255)
                      gCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAg                                                                              <  1:1085905/100‑1 (MQ=255)
                      gCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAg                                                                              <  1:2063915/100‑1 (MQ=255)
                      gCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAg                                                                              >  2:1827715/1‑100 (MQ=255)
                         gCACTTGCGGTAGTGGGCTTCAAAGTTAAAGACTTTGCCCTCCGGACGGTGTAACAATTCATTCCTGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATc                                                                           <  2:320438/100‑1 (MQ=255)
                         gCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATc                                                                           <  2:1185292/100‑1 (MQ=255)
                         acACTGGCGGTACTGGGCTTCAAAGTTAAAGAATGTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATc                                                                           <  2:1475294/99‑1 (MQ=255)
                           aCTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGa                                                                         <  1:957803/100‑1 (MQ=255)
                              ggCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAAt                                                                      >  2:272057/1‑100 (MQ=255)
                              ggCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAAt                                                                      >  2:277322/1‑100 (MQ=255)
                                cGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCt                                                                    >  1:1061868/1‑100 (MQ=255)
                                cGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCt                                                                    >  1:442135/1‑100 (MQ=255)
                                 ggTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCtt                                                                   >  1:638049/1‑100 (MQ=255)
                                   tACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCtttt                                                                 <  1:324217/100‑1 (MQ=255)
                                    aCTGGGCTTCAAAGTTAAAGAATTTGGCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCttttt                                                                <  2:2526048/100‑1 (MQ=255)
                                    aCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCttttt                                                                <  1:493959/100‑1 (MQ=255)
                                       gggCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTAcc                                                             >  2:1189595/1‑100 (MQ=255)
                                       gggCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTAcc                                                             <  1:1303648/100‑1 (MQ=255)
                                           ttCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTAccgccg                                                         <  1:1379676/100‑1 (MQ=255)
                                           ttCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTAccgccg                                                         >  1:1722258/1‑100 (MQ=255)
                                            tCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACcgccgc                                                        >  1:2292934/1‑100 (MQ=255)
                                             cAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGTCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACcgccgc                                                        >  2:1033866/1‑99 (MQ=255)
                                              aaaGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGa                                                      >  1:1430980/1‑100 (MQ=255)
                                               aaGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGAt                                                     >  1:1218254/1‑100 (MQ=255)
                                               aaGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGAt                                                     >  1:1799128/1‑100 (MQ=255)
                                                aGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATg                                                    >  1:2378672/1‑100 (MQ=255)
                                                aGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATg                                                    >  1:1135653/1‑100 (MQ=255)
                                                  ttAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATggg                                                  >  2:207822/1‑100 (MQ=255)
                                                  ttAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATggg                                                  <  1:2002738/100‑1 (MQ=255)
                                                  ttAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATggg                                                  >  2:1935135/1‑100 (MQ=255)
                                                   tAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGgt                                                 >  1:993220/1‑100 (MQ=255)
                                                   tAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGgt                                                 <  1:464914/100‑1 (MQ=255)
                                                    aaaGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGgtt                                                >  1:331891/1‑100 (MQ=255)
                                                     aaGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGgttg                                               <  2:352347/100‑1 (MQ=255)
                                                     aaGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGgttg                                               <  2:351178/100‑1 (MQ=255)
                                                       gAAGTTGCCCGCCGGACGGTGTAACAATGCATTTCGGTCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGgttgtt                                             <  2:131038/100‑1 (MQ=255)
                                                        aaTTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGttgttg                                            <  1:753010/100‑1 (MQ=255)
                                                         aTTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGa                                           <  2:647613/100‑1 (MQ=255)
                                                           ttGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAgg                                         <  1:1785974/100‑1 (MQ=255)
                                                           ttGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAgg                                         <  1:1477130/100‑1 (MQ=255)
                                                           ttGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAg                                          >  2:971194/1‑99 (MQ=255)
                                                            tGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGt                                        <  2:86923/100‑1 (MQ=255)
                                                            tGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGt                                        <  1:406674/100‑1 (MQ=255)
                                                             gCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTc                                       <  2:1350051/100‑1 (MQ=255)
                                                              cccGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCt                                      >  1:1163718/1‑100 (MQ=255)
                                                                 gccgGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATATTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGAc                                   <  2:2520886/100‑1 (MQ=255)
                                                                     gACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGtt                               >  2:893647/1‑100 (MQ=255)
                                                                       cGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCg                             >  2:2262007/1‑100 (MQ=255)
                                                                         gtgtAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGcc                           <  2:1020312/100‑1 (MQ=255)
                                                                          tgtAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGccc                          >  1:1638814/1‑100 (MQ=255)
                                                                          tgtAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGccc                          >  1:180702/1‑100 (MQ=255)
                                                                           gtAACAATGCATTCCGGCCTGCAGTGCTGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGcccc                         <  2:707695/100‑1 (MQ=255)
                                                                           gtAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGcccc                         <  1:2343372/100‑1 (MQ=255)
                                                                            tAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCa                        <  1:2546534/100‑1 (MQ=255)
                                                                            tAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCa                        >  2:2413756/1‑100 (MQ=255)
                                                                             aaCAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGcc                           <  1:809181/96‑1 (MQ=255)
                                                                               cAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTCTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCatat                     >  2:1251007/1‑100 (MQ=255)
                                                                                   tatctCCGGCCTGCACTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTCGTTGAGGTCTGACCGTTCGCCCCATATAGCg                 >  2:321303/5‑100 (MQ=39)
                                                                                    cATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGt                <  2:1931628/100‑1 (MQ=255)
                                                                                     aTTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTc               >  1:2185181/1‑100 (MQ=255)
                                                                                      ttCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCg              <  1:1175791/100‑1 (MQ=255)
                                                                                       tCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCgg             <  2:1613336/100‑1 (MQ=255)
                                                                                       cccGGCCTGCACTGCAGGTCGGAGATAATCGTCAGATCGAAATATTTTGACGGGCGCGATGGGTTGTTGAGGTCTGACCTTTCGCACTATATAGCGTCgg             >  2:381660/2‑100 (MQ=255)
                                                                                        ccGGCCTGCACTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGt            >  1:1644064/1‑100 (MQ=255)
                                                                                             cTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTg       >  1:966624/1‑100 (MQ=255)
                                                                                             cTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTg       >  1:966042/1‑100 (MQ=255)
                                                                                             cTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCACCCCATATAGCGTCGGTTCCTg       <  2:1131272/100‑1 (MQ=255)
                                                                                              tgcagtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGa      >  2:1332174/1‑100 (MQ=255)
                                                                                                cagtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATg    >  2:1144878/1‑100 (MQ=255)
                                                                                                 agtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGc   <  1:317582/100‑1 (MQ=255)
                                                                                                  gtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCg  <  1:277326/100‑1 (MQ=255)
                                                                                                  gtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCACCCTATAGAGTCGGCTCCtgctgc   >  1:2394268/1‑99 (MQ=255)
                                                                                                  |                                                                                                   
GGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCG  >  NC_000913/1129296‑1129493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: