Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,196,803 A→C 17.0% intergenic (+430/+64) icd → / ← ymfD isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase/e14 prophage; putative SAM‑dependent methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,196,8030AC17.0% 262.3 / 47.6 111intergenic (+430/+64)icd/ymfDisocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase/e14 prophage; putative SAM‑dependent methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (43/49);  new base C (10/9);  total (53/58)
Fisher's exact test for biased strand distribution p-value = 8.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

TATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATG  >  NC_000913/1196704‑1196901
                                                                                                   |                                                                                                  
tATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGa                                                                                                    >  1:1325460/1‑100 (MQ=255)
    aaCTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAgcgc                                                                                                >  2:1032807/1‑100 (MQ=255)
     actactCACCCGGGAGTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACAAAAAAAAAAAAAAAAAGGCCGCGAgcg                                                                                                 <  2:2087173/98‑1 (MQ=255)
       tactCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGc                                                                                             >  2:890811/1‑100 (MQ=255)
         ctCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGCTCGCGGcct                                                                                           >  1:200567/1‑99 (MQ=255)
         cgcACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGCTCGCGGcct                                                                                           >  1:2373552/3‑99 (MQ=255)
           cACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCt                                                                                         <  2:2528058/100‑1 (MQ=255)
           cACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCt                                                                                         >  1:2237344/1‑100 (MQ=255)
            aCCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGCTCGCGGcctttt                                                                                        <  1:548461/100‑5 (MQ=255)
             cccGGTACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGCTCGCGGccttttt                                                                                       <  2:359966/100‑6 (MQ=255)
             cccGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAACAAAGGCCGCGAGCGCGGCCCCTTc                                                                                       >  2:1008837/1‑100 (MQ=255)
             cccGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGCTCGCGGccttttt                                                                                       <  1:2258678/100‑6 (MQ=255)
             cccGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCtt                                                                                        >  1:515791/1‑99 (MQ=255)
             cccGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTc                                                                                       <  1:692368/100‑1 (MQ=255)
             cccGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTc                                                                                       <  2:1210929/100‑1 (MQ=255)
              ccGGGACTCTCCAGGGTACAGCCAACAGGCATTGGTTGGAATCACCTTAGCGTTCAGGTACATGCGCAATGTAAAAAAGGCCGCGCTCGCGGcctttttt                                                                                      <  2:1721814/100‑7 (MQ=255)
              ccGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGCTCGCGGcctttttt                                                                                      <  1:2135657/100‑7 (MQ=255)
              ccGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTca                                                                                      <  2:1664889/100‑1 (MQ=255)
               cGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGCTCGCGGcctttttta                                                                                     <  2:2373537/100‑8 (MQ=255)
               cGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTcac                                                                                     <  1:2340403/100‑1 (MQ=255)
                gggACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGCTCGCGGccttttttac                                                                                    <  1:1150647/100‑9 (MQ=255)
                gggACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGCTCGCGGccttttttac                                                                                    <  1:1267409/100‑9 (MQ=255)
                gggACTCGCCAGGGGACAGCCAACAGGCATTGGGAGCAATCACCTTCGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGCTCGCGGccgtttttac                                                                                    <  2:1341685/100‑9 (MQ=255)
                  gACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCacata                                                                                  <  2:177979/100‑1 (MQ=255)
                  gACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATATAAAAAAGACCGCGAGCGCGGCCCCTTCacata                                                                                  >  2:935559/1‑100 (MQ=255)
                    cTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCacataca                                                                                >  2:290379/1‑100 (MQ=255)
                    cTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCacataca                                                                                >  1:1661603/1‑100 (MQ=255)
                     tCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCTCTTctca                                                                                    <  2:1320757/95‑1 (MQ=255)
                           ggggACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAg                                                                         >  2:750015/1‑100 (MQ=255)
                             ggACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTa                                                                       <  2:358260/100‑1 (MQ=255)
                              gACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTAc                                                                      <  1:771516/100‑1 (MQ=255)
                              gACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTAc                                                                      <  2:2071199/100‑1 (MQ=255)
                                cAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTg                                                                    >  1:1653159/1‑100 (MQ=255)
                                 aGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTga                                                                   <  2:362611/100‑1 (MQ=255)
                                 aGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTga                                                                   <  2:1197628/100‑1 (MQ=255)
                                  gCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTgag                                                                  >  1:2380860/1‑100 (MQ=255)
                                  gCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTgag                                                                  <  2:780963/100‑1 (MQ=255)
                                  gCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTgag                                                                  >  1:1163254/1‑100 (MQ=255)
                                   ccAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTgaga                                                                 <  2:699069/100‑1 (MQ=255)
                                   ccAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTgaga                                                                 >  2:2000919/1‑100 (MQ=255)
                                    cAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGAc                                                                >  1:281735/1‑100 (MQ=255)
                                      aCAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTg                                                              <  1:537614/100‑1 (MQ=255)
                                      aCAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTg                                                              >  1:992875/1‑100 (MQ=255)
                                       cAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGt                                                             <  2:1461735/100‑1 (MQ=255)
                                         ggCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGttt                                                           <  1:2009072/100‑1 (MQ=255)
                                          gCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGttt                                                           <  2:317544/99‑1 (MQ=255)
                                            aTTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAAc                                                        >  2:1293570/1‑100 (MQ=255)
                                             ttGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAAcc                                                       <  2:902821/100‑1 (MQ=255)
                                             ttGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAAcc                                                       <  1:1240927/100‑1 (MQ=255)
                                               gggTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTACCCTa                                                     >  2:1183055/1‑100 (MQ=255)
                                                 gTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAgg                                                   >  1:303102/1‑100 (MQ=255)
                                                 gTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAgg                                                   >  1:2051098/1‑100 (MQ=255)
                                                     aaTCACCTTAGCGTTCAGGTACATGCGGAATGTAAAACAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAat                                               >  2:1366785/1‑100 (MQ=255)
                                                     aaTCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAat                                               >  1:404474/1‑100 (MQ=255)
                                                      aTCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAatt                                              <  2:499560/100‑1 (MQ=255)
                                                        cACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAattat                                            <  1:173746/100‑1 (MQ=255)
                                                          ccTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAAttatta                                          <  1:1964013/100‑1 (MQ=255)
                                                          ccTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAAttatta                                          >  1:2542214/1‑100 (MQ=255)
                                                            ttAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATc                                        <  1:1552317/100‑1 (MQ=255)
                                                            ttAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATc                                        >  1:1213575/1‑100 (MQ=255)
                                                                cGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGtat                                    <  2:1163246/100‑1 (MQ=255)
                                                                cGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGtat                                    >  1:1829341/1‑100 (MQ=255)
                                                                cGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGtat                                    <  1:2285367/100‑1 (MQ=255)
                                                                  ttCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGtatta                                  <  2:1960650/100‑1 (MQ=255)
                                                                      ggTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATAtt                              >  1:686722/1‑100 (MQ=255)
                                                                        tACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGc                            <  2:1143762/100‑1 (MQ=255)
                                                                          cATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCAt                          >  1:2491128/1‑100 (MQ=255)
                                                                           aTGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGGATTATTATCGTATTATATTGCAtt                         >  2:377677/1‑100 (MQ=255)
                                                                              cGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACa                      <  1:643620/100‑1 (MQ=255)
                                                                                 aaTGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAAc                    <  2:2212170/99‑1 (MQ=255)
                                                                                  aTGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACTTCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATCACAACTc                  >  2:1374595/1‑100 (MQ=255)
                                                                                  aTGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTc                  <  2:323378/100‑1 (MQ=255)
                                                                                  aTGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTc                  <  1:637881/100‑1 (MQ=255)
                                                                                  aTGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTc                  <  1:303356/100‑1 (MQ=255)
                                                                                   atgtgaaggggCCGCGCTCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTcc                 >  1:1115405/10‑100 (MQ=255)
                                                                                      tgaaggggCCGCGCTCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCaa              >  2:1573419/7‑100 (MQ=255)
                                                                                      aaaaaaGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCaa              >  1:2119135/1‑100 (MQ=255)
                                                                                       aaaaaGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAAc             >  1:513209/1‑100 (MQ=255)
                                                                                       aaaaaGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAAc             <  1:635830/100‑1 (MQ=255)
                                                                                       aaaaaGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAAc             >  1:205038/1‑100 (MQ=255)
                                                                                       aaaaaGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAAc             <  2:1407114/100‑1 (MQ=255)
                                                                                         aggggCCGCGCTCGCGGCCCCTTCACATACATCTTTAGTACTGAGCCTGTTTAACCTAGGGAATTATTATAGTATTATATTGCATTACAACTCCAAACAg           >  2:1910465/4‑100 (MQ=255)
                                                                                         aggggCCGCGCTCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAg           >  1:1410733/4‑100 (MQ=255)
                                                                                          aaGGCCGCGAGCGCGGCACCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCCACAg           >  1:1264357/1‑99 (MQ=255)
                                                                                           gggCCGCGCTCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTg         >  2:1510523/2‑100 (MQ=255)
                                                                                           gggCCGCGCTCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTg         >  1:959766/2‑100 (MQ=255)
                                                                                           aGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTg         <  2:1318797/100‑1 (MQ=255)
                                                                                            ggCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGa        <  2:176720/100‑1 (MQ=255)
                                                                                             gCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGAc       <  2:2195050/100‑1 (MQ=255)
                                                                                             gCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGAc       >  1:2045566/1‑100 (MQ=255)
                                                                                             gCCACGAGCGCGGCCCCTTCACATACATCTTTAGTACTGTGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTg         >  2:1755340/1‑98 (MQ=255)
                                                                                              ccGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCCACAGTGACa      >  1:2443289/1‑100 (MQ=255)
                                                                                              ccGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACa      <  2:2476640/100‑1 (MQ=255)
                                                                                              ccGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACa      <  1:1278308/100‑1 (MQ=255)
                                                                                              ccGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAAATCCCAACCGTGACa      >  1:212024/1‑100 (MQ=255)
                                                                                               cgcgAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACat     >  2:30341/1‑100 (MQ=255)
                                                                                               cgcgAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACat     <  1:1361744/100‑1 (MQ=255)
                                                                                                gcgAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACata    <  1:1877094/100‑1 (MQ=255)
                                                                                                 cgAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACatat   <  1:833680/100‑1 (MQ=255)
                                                                                                  gAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTTTTTAACCTAGTGAATTATTATCGTATTATATTTCATTACATCTTCCAACAGTGACATATg  <  2:559590/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
TATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATG  >  NC_000913/1196704‑1196901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: