Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,197,340 | T→C | 100% | T65A (ACT→GCT) | ymfD ← | e14 prophage; putative SAM‑dependent methyltransferase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,197,340 | 0 | T | C | 98.8% | 271.5 / ‑5.2 | 81 | T65A (ACT→GCT) | ymfD | e14 prophage; putative SAM‑dependent methyltransferase |
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (38/42); minor base G (1/0); total (39/42) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.81e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 |
CATTTTTATAATATCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GT‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATAT > NC_000913/1197244‑1197435 | cATTTTTATAATATCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCACTCTTACAAGTTGCATTTTCGAGTCTAGAAAA‑GC‑AAc > 2:1726760/1‑100 (MQ=255) tataATATCGTGGGACATAGTCAATAATTTTAGTTTTAATTCTTCTAATAATTTGCTCTCTTTCAAGTTAACTTTTCGAGTCTAGAAAA‑GC‑AACTtcatc > 2:455897/1‑100 (MQ=255) ataataTCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTtcatca > 1:1184862/1‑100 (MQ=255) ataataTCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTtcatca > 2:2493445/1‑100 (MQ=255) taataTCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCaa > 1:1438911/1‑100 (MQ=255) taataTCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCaa > 2:313142/1‑100 (MQ=255) atatCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAAt > 2:2554303/1‑100 (MQ=255) atatCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAAt > 1:434286/1‑100 (MQ=255) atatCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAAt < 2:2236470/100‑1 (MQ=255) atatCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAAt < 1:1510534/100‑1 (MQ=255) tatCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAAtt < 2:1735211/100‑1 (MQ=255) cGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTAc < 2:697438/100‑1 (MQ=255) gTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTAc < 2:412238/99‑1 (MQ=255) gTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACt < 1:973704/100‑1 (MQ=255) tcatacacAGTCAATACTTTTACTTTTAATTCCCCTAATATTGTACTCTCTTTCAAGTTACTTTTTCGAGTCTACAAAA‑GC‑ACCTTTCACTAATTTAc < 2:2308780/94‑1 (MQ=255) tGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTg < 2:290862/100‑1 (MQ=255) gACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGAtt < 1:1124406/100‑1 (MQ=255) gACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGAtt > 2:1882905/1‑100 (MQ=255) gACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTATCTTTCAAGTTGCCTTTTCGAGTCTAGAACA‑GC‑AACTTCATCAAATTTACTGaat > 2:843296/1‑98 (MQ=255) aCATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCCCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACt < 2:230311/95‑1 (MQ=255) tAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGGAAA‑GC‑AACTTCATCAAATTTACTGATTAAtt < 1:1726772/100‑1 (MQ=255) tAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAAtt > 2:1731449/1‑100 (MQ=255) tAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAAtt > 1:2144996/1‑100 (MQ=255) aGTCAATAATTTCAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATtc > 1:1686957/1‑100 (MQ=255) gTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATtca > 1:1121218/1‑100 (MQ=255) cAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTGTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATtcatc > 1:1053497/1‑100 (MQ=255) cAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATtcatc > 1:309515/1‑100 (MQ=255) cAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATtcatc < 1:356666/100‑1 (MQ=255) cAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTCACTGATTAATtcatc < 2:2304772/100‑1 (MQ=255) cAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCAAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATtcatc > 2:1645112/1‑100 (MQ=255) aataatTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATtcatca < 1:1284961/100‑1 (MQ=255) ataatTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑ATCTTCATCAAATTTACTGATTAATTCATCAg < 2:1686945/100‑1 (MQ=255) natTTTAGTTTTAATTCCTCTAATAATTTGCTATCTTTCAAGTTGCATTTTCTATTATATAAAA‑GC‑AACTTAATCAAATTTACTGATTAATTAATCAGaa > 2:2467665/2‑100 (MQ=255) ttttAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAata < 1:758565/100‑1 (MQ=255) ttttAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAata < 2:2165216/100‑1 (MQ=255) ttAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATc < 1:2290222/100‑1 (MQ=255) tttAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGt < 1:1722857/100‑1 (MQ=255) tCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCg < 1:2065403/100‑1 (MQ=255) ccTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGc < 1:233481/100‑1 (MQ=255) ctctAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCa > 1:635415/1‑100 (MQ=255) ctAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGc > 2:2288046/1‑100 (MQ=255) taataaTTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGcc < 2:1762785/100‑1 (MQ=255) aataatTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCa > 1:928468/1‑100 (MQ=255) aataatTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCa > 1:792293/1‑100 (MQ=255) aataatTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCa > 2:61736/1‑100 (MQ=255) aataatTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCa > 2:780641/1‑100 (MQ=255) atTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAAt < 1:2319026/100‑1 (MQ=255) ttGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCa < 1:2267483/100‑1 (MQ=255) tGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCaa < 1:291816/100‑1 (MQ=255) ctctctTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAgg < 1:608630/100‑1 (MQ=255) ctctctTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCAGCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAgg < 2:1437091/100‑1 (MQ=255) tctctTTCAAGTTGCCTTTTTTATTTTTTTAAA‑GC‑AACTTCATCAAATTTACTGATTAATTTATCAGAATATCTAAGTTTTCCGCTGCCAAAATc > 2:1524809/1‑95 (MQ=255) tctctTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAggg < 2:1268169/100‑1 (MQ=255) tctctTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAggg > 2:2432971/1‑100 (MQ=255) tctctTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAgcg < 1:1731461/100‑3 (MQ=255) tctctTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTGCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAggg < 2:2399925/100‑1 (MQ=255) tttCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTg < 2:366325/100‑1 (MQ=255) gatgaaGTTG‑CTTTTCTAGACTCGAAAA‑GGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTACGCAGCCAAAATCAAGGGCg > 2:2524248/5‑98 (MQ=21) ttCACGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATCCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTgg < 1:756709/100‑1 (MQ=255) ttCAAGTTGCCTTTTCGAGTCTAGCAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAgg > 2:1127097/1‑94 (MQ=255) ttCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTgg < 2:2141418/100‑1 (MQ=255) ttCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTgg < 1:61737/100‑1 (MQ=255) ttCAAGTTGCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTgg < 1:1529677/100‑1 (MQ=255) aaGTTG‑CTTTTCTAGACTCGAAAAGGC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCa > 1:438681/1‑100 (MQ=37) gCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCAttttt > 1:1624774/1‑100 (MQ=255) gCCTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCAttttt > 1:2104027/1‑100 (MQ=255) ccTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTtc > 1:2032703/1‑100 (MQ=255) cTTTTCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCTGCAGCCAAAATCAAGGGCGTGGCCATTTTtct < 1:2427304/100‑1 (MQ=255) ttttCGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTtctc < 2:2287753/100‑1 (MQ=255) cGAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATg > 2:162411/1‑100 (MQ=255) gAGTCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGc < 2:2352224/100‑1 (MQ=255) tCTAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTc > 2:452523/1‑100 (MQ=255) tAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTa > 1:2066452/1‑100 (MQ=255) tAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTa > 1:2040096/1‑100 (MQ=255) tAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATAAAGGGCGTGGCCATTTTTCTCAATGCTTCTa > 2:647246/1‑100 (MQ=255) tAGAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGCTTTCCGCAGCCAAAATCAAGGACGTGGCCATTTTTCTCAAtcgtgata > 2:1582271/1‑93 (MQ=255) agcaaa‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGTAGCCAAGATCAAGGGCGTGGCCAGTTTTCTCAATGGTTCTaa < 2:464121/97‑1 (MQ=255) gAAAA‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAat < 2:406727/100‑1 (MQ=255) aaaa‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAata < 1:228766/100‑1 (MQ=255) aaaa‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAata < 2:1652084/100‑1 (MQ=255) aaa‑GC‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAatat > 2:873929/1‑100 (MQ=255) | CATTTTTATAATATCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAA‑GT‑AACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATAT > NC_000913/1197244‑1197435 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |