BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,197,739	A→G	100%	S167P (TCC→CCC)	ymfE ←	e14 prophage; putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,197,739	0	A	G	100.0%	290.3 / ‑6.7	89	S167P (TCC→CCC)	ymfE	e14 prophage; putative inner membrane protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (50/37); total (51/38)

Only 100 of 103 total aligned reads displayed.

CTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTAC  >  NC_000913/1197643‑1197835

ctGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGAATATTAGGAAGGGAAt                                                                                               >  1:2525589/1‑100 (MQ=255)

  gagaATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAACAGATTATTAGGAAGGGAATTa                                                                                             >  2:1074969/1‑100 (MQ=255)

  gagaATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTa                                                                                             >  1:1427096/1‑100 (MQ=255)

   agaATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTaa                                                                                            <  1:2335856/100‑1 (MQ=255)

    gaATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAAt                                                                                           >  2:1873063/1‑100 (MQ=255)

    gaATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAAt                                                                                           <  1:676489/100‑1 (MQ=255)

     aaTGATAAAATTAAAAAAAAGAATAAAGACAGCAAAGAAAAAAAAAAAAAAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATg                                                                                          <  2:1172970/100‑1 (MQ=255)

      aTGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGc                                                                                         <  2:866118/100‑1 (MQ=255)

      aTGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGc                                                                                         >  1:1105701/1‑100 (MQ=255)

       tGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCa                                                                                        >  1:2442060/1‑100 (MQ=255)

         aTAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAAt                                                                                      >  1:1892343/1‑100 (MQ=255)

          tAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAAtt                                                                                     >  1:416532/1‑100 (MQ=255)

           aaaaTTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTa                                                                                    >  2:1857667/1‑100 (MQ=255)

                 aCAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGaaa                                                                              <  1:236897/100‑1 (MQ=255)

                  cAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATAAGGAAGGGAATTAATGCAATTATAGaaaa                                                                             >  2:2256124/1‑100 (MQ=255)

                   aaaaaaGAATAAAGACGGCAAAGAACCTTCAACATGAAAAATATCGATTTGTTTTCAAAAAAAGATGATTAGGAAGGAAATTAATGCAATTATCGaaaa                                                                             >  2:1069612/1‑99 (MQ=255)

                    aaaaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAAtt                                                                           >  1:1863055/1‑100 (MQ=255)

                    aaaaaGAATAAAGACAGCAAAGAACCTTCAACAGGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTATGAAGGGAATTAATGCAATTATCGAAAAtt                                                                           >  2:1540344/1‑100 (MQ=255)

                      aaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCa                                                                         >  2:418009/1‑100 (MQ=255)

                      aaaGAATAAAGACAGCAAAGAAACTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCa                                                                         <  2:1902748/100‑1 (MQ=255)

                        aGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaa                                                                       <  2:2525574/100‑1 (MQ=255)

                        aGAATAAAGACAGAAAAGAACCTTAAACATTAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaa                                                                       <  2:2145443/100‑1 (MQ=255)

                          aaTAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaaaa                                                                     <  1:899908/100‑1 (MQ=255)

                           aTAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaaaaa                                                                    >  1:802699/1‑100 (MQ=255)

                           aTAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaaaaa                                                                    >  1:555784/1‑100 (MQ=255)

                           aTAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaaaaa                                                                    >  2:706357/1‑100 (MQ=255)

                           aTAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaaaaa                                                                    >  1:1556713/1‑100 (MQ=255)

                             taaGAAAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGAAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAata                                                                  <  2:2392455/99‑1 (MQ=255)

                             aaaGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAata                                                                  >  1:1447835/1‑100 (MQ=255)

                              aaGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAatat                                                                 >  1:46871/1‑100 (MQ=255)

                              aaGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAatat                                                                 <  1:438087/100‑1 (MQ=255)

                              aaGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAataa                                                                 >  1:2392469/1‑99 (MQ=255)

                                    gCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGCTTATTAGGAAGGGACTTAATGCAATTCTCGAAAATTCaaaacctctcca                                                              >  2:2008620/1‑89 (MQ=255)

                                    gCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaa                                                           >  2:1222934/1‑100 (MQ=255)

                                     cAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaaa                                                          >  2:626252/1‑100 (MQ=255)

                                       aaaaaaattCAACAAGAAAAATATCCATTTGTTTGAAAAAAAAGATTATTAGGAAAGGAATTAATGCAATTAAAGAAAAAAAAAAAAATATCCAAAAAt                                                         <  2:146583/92‑1 (MQ=255)

                                       aaGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATa                                                        <  1:2493460/100‑1 (MQ=255)

                                       aaGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATa                                                        >  2:2295464/1‑100 (MQ=255)

                                        aGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCGAAAATAg                                                       <  1:1344461/100‑1 (MQ=255)

                                        aGAAACTTCAACATGAAAAATATCCATTTAATTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATAGAAAAATCAAAAAATATCCAAAAATAg                                                       <  2:1306637/100‑1 (MQ=255)

                                          aaCCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGt                                                      <  2:592788/99‑1 (MQ=255)

                                            ccTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGtata                                                   >  1:1015638/1‑100 (MQ=255)

                                            ccTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGtata                                                   >  1:1831144/1‑100 (MQ=255)

                                            ccTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGtata                                                   >  1:259868/1‑100 (MQ=255)

                                             cTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATAc                                                  <  1:1207731/100‑1 (MQ=255)

                                                cAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAGATTCAAAAAATATCCAAAAATAGTATACtt                                                >  1:756106/1‑99 (MQ=255)

                                                  aCATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTAt                                             >  1:856327/1‑100 (MQ=255)

                                                  aCATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTAt                                             >  1:2128509/1‑100 (MQ=255)

                                                    aTGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATAAAAAAATAGTATACTTTAttg                                           >  2:1122487/1‑99 (MQ=255)

                                                     tGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGTAAGGGAATTAATGCAATTATCAAAAATTCAAAAAATATCCAAAAATAATATACTTTATTcc                                          >  2:2053283/1‑100 (MQ=255)

                                                     tGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTcc                                          <  1:1359176/100‑1 (MQ=255)

                                                     tGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTcc                                          <  1:2373892/100‑1 (MQ=255)

                                                      gAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCa                                         <  1:1680396/100‑1 (MQ=255)

                                                       aaaaaTATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCag                                        <  1:757266/100‑1 (MQ=255)

                                                       aaaaaTATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCag                                        >  2:159671/1‑100 (MQ=255)

                                                        aaaaTATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCaga                                       >  1:612274/1‑100 (MQ=255)

                                                          aaTATCCATTTAATAAAAAAAAAAGATTATAAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCagaag                                     <  2:1781012/100‑1 (MQ=255)

                                                            tatCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAagag                                   <  1:1041151/100‑1 (MQ=255)

                                                               ccATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTc                                <  1:1882919/100‑1 (MQ=255)

                                                               ccATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTc                                <  1:1031434/100‑1 (MQ=255)

                                                               caaTTTGTTTGCAAAAAAAGATTATTAGGAAGGTAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTc                                <  2:393245/98‑1 (MQ=255)

                                                                cATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCa                               >  1:298292/1‑100 (MQ=255)

                                                                cATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCa                               >  1:1200591/1‑100 (MQ=255)

                                                                 aTTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCaa                              <  2:494092/100‑1 (MQ=255)

                                                                 aTTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCaa                              <  1:1932229/100‑1 (MQ=255)

                                                                     ntttgCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAAtata                          >  2:2277770/2‑100 (MQ=255)

                                                                       ttgCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAAtatan                         <  1:2467679/99‑2 (MQ=255)

                                                                        tgCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATaa                         >  2:1580284/1‑98 (MQ=255)

                                                                         gCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGt                      >  1:2099584/1‑100 (MQ=255)

                                                                         gCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGt                      >  1:1527165/1‑100 (MQ=255)

                                                                          cAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGtt                     >  2:2107953/1‑100 (MQ=255)

                                                                          cAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGtt                     >  2:2222960/1‑100 (MQ=255)

                                                                          cAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGtt                     >  1:955206/1‑100 (MQ=255)

                                                                          aaaaaaaaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGtt                     <  2:155648/99‑1 (MQ=255)

                                                                           aaaaaaaGATTATTAGAGAGGGAATGAAGTCGATTAAAGAAAATTGAAGATGTAACCAAAAGTAGTGAACGTGATTTGATAAGAGTTCAGtatatag                       >  2:246645/1‑94 (MQ=255)

                                                                           aaaaaaaGATTATAAGGAAGGGAATTAATGCAATTATCAAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGATATCAATATAATGttt                    >  2:1585892/1‑100 (MQ=255)

                                                                            aaaaaaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTg                   <  2:1897295/100‑1 (MQ=255)

                                                                            aaaaaaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTg                   <  1:735793/100‑1 (MQ=255)

                                                                              aaaaGATTATTAGGAAGGGAATGAATGCAATTATCGAAAATTCAAAAAATATCTAAAAGTAGTATACTTTATTTCAGAAGAGTTCAATATAATGttt                    >  2:1665887/1‑97 (MQ=255)

                                                                                aaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCtt               >  2:1282302/1‑100 (MQ=255)

                                                                                 aGATTATTAGGAAGTGAATTAATTCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTc              <  1:851970/100‑1 (MQ=255)

                                                                                 aGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTc              <  2:1261678/100‑1 (MQ=255)

                                                                                   attattAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCaa            >  2:647156/1‑100 (MQ=255)

                                                                                   attattAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCa             >  2:1200197/1‑99 (MQ=255)

                                                                                     tattaGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAAtt          <  1:1282310/100‑1 (MQ=255)

                                                                                      attaGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAAttt         >  2:826189/1‑100 (MQ=255)

                                                                                         aggaagggAATTAATGTAATCAGCGTAAATTCAAAAAAAAAAAAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTc      <  2:214800/100‑1 (MQ=255)

                                                                                         aggaagggAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAAttttt       >  2:2236459/1‑99 (MQ=255)

                                                                                         aggaagggAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTc      <  1:480719/100‑1 (MQ=255)

                                                                                         aggaagggAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTc      <  1:163240/100‑1 (MQ=255)

                                                                                         aggaagggAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTc      <  2:330867/100‑1 (MQ=255)

                                                                                         aggaagggAATTAATGCAATTATCGAAAATTCAAAAAAAAAAAAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTc      <  2:1195132/100‑1 (MQ=255)

                                                                                          ggaagggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCt     >  2:1885594/1‑100 (MQ=255)

                                                                                          ggaagggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAAAATAATGTTTGTCTTCAAttttt       >  2:2532785/1‑98 (MQ=255)

                                                                                           gaagggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCtt    <  2:560809/100‑1 (MQ=255)

                                                                                           gaagggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCtt    <  1:236462/100‑1 (MQ=255)

                                                                                            aagggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTa   >  1:596497/1‑100 (MQ=255)

                                                                                             agggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTa   >  2:421397/1‑99 (MQ=255)

                                                                                             agggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTATATTCCAGAAGAATTCAATATAATGTTTGTCTTCAATTTTTcat    >  2:1446974/1‑96 (MQ=255)

                                                                                             agggaATTAATGCAATTATCGAAAATTAAAAAAATAAAAAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTAc  <  2:1831130/100‑1 (MQ=255)

CTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTAC  >  NC_000913/1197643‑1197835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`