Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,232,481 C→T 16.3% intergenic (+27/+19) umuC → / ← dsbB translesion error‑prone DNA polymerase V subunit; DNA polymerase activity/oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,232,4810CT16.3% 256.3 / 33.8 110intergenic (+27/+19)umuC/dsbBtranslesion error‑prone DNA polymerase V subunit; DNA polymerase activity/oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I
Reads supporting (aligned to +/- strand):  ref base C (57/34);  new base T (6/12);  total (63/47)
Fisher's exact test for biased strand distribution p-value = 3.48e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 112 total aligned reads displayed.

TGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCG  >  NC_000913/1232383‑1232578
                                                                                                  |                                                                                                 
tGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCg                                                                                                  >  2:956766/1‑100 (MQ=255)
   cAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAg                                                                                               >  1:670366/1‑100 (MQ=255)
   cAGATAAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGTGGCAGGAAAAAAGCGCTCCCGCAg                                                                                               <  2:135149/100‑1 (MQ=255)
    aGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAgg                                                                                              <  1:2057294/100‑1 (MQ=255)
       tGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAgc                                                                                           >  1:556206/1‑100 (MQ=255)
        gAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAgcg                                                                                          >  1:953185/1‑100 (MQ=255)
         aaGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAgcgc                                                                                         >  2:807974/1‑100 (MQ=255)
            cGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCCGGAAAAAAGCGCTCCCGCAGGAGCGCCGa                                                                                      >  2:86243/1‑100 (MQ=255)
             gAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgctttt                                                                                     <  2:1308725/100‑5 (MQ=255)
              aGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttttt                                                                                    <  2:2457058/100‑6 (MQ=255)
              aGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttttt                                                                                    <  2:5906/100‑6 (MQ=255)
              aGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAAt                                                                                    >  1:1910367/1‑100 (MQ=255)
               gCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgctttttt                                                                                   <  1:1571869/100‑7 (MQ=255)
               gCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATg                                                                                   >  1:416543/1‑100 (MQ=255)
               gCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATg                                                                                   >  1:576853/1‑100 (MQ=255)
               gCCATGCGTTCACCACGTTATACGACTCGAAGTTCTGATTTACTTAGGGTCAAATAAATATTGCGGCAGGAAAAAAGCGCTCCTGCGGGAtcgctttt                                                                                     <  2:949728/98‑5 (MQ=255)
                ccATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATgg                                                                                  <  1:620857/100‑1 (MQ=255)
                 cATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttttttcc                                                                                 <  1:2390945/100‑9 (MQ=38)
                 cATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGa                                                                                 >  1:1933215/1‑100 (MQ=255)
                 cATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAAGAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttttttcc                                                                                 <  2:499884/100‑9 (MQ=38)
                  aTGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttttttcct                                                                                <  2:221365/100‑10 (MQ=38)
                  aTGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttttttcct                                                                                <  2:92252/100‑10 (MQ=38)
                   tGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttttttcct                                                                                <  2:1718526/99‑10 (MQ=38)
                   tGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGAtt                                                                               <  2:1408052/100‑1 (MQ=255)
                    gCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGTCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTa                                                                              >  2:1800770/1‑100 (MQ=255)
                    gCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTa                                                                              >  1:1975619/1‑100 (MQ=255)
                    gCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTa                                                                              >  2:19059/1‑100 (MQ=255)
                    gCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCCCCCGCAGGAGCGCCGAAt                                                                                    >  2:614903/1‑94 (MQ=255)
                     cTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAg                                                                             <  1:38974/100‑1 (MQ=255)
                     cTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAg                                                                             <  2:193714/100‑1 (MQ=255)
                     cTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAg                                                                             >  2:2423838/1‑100 (MQ=255)
                      tgtCACCACGTTATACAACGCGCAGTTCTGATTTATGGAGCGCCAAATAAATATAGCGGCAGCAAAAAAGCGCTCCCGCATGAGCGCCGAATGGATTAGc                                                                            <  2:64271/98‑1 (MQ=255)
                       ttCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCg                                                                           <  1:1614857/100‑1 (MQ=255)
                       ttCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCg                                                                           <  1:1655082/100‑1 (MQ=255)
                         caccacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGAc                                                                         >  2:1530722/1‑100 (MQ=255)
                         caccacGTTATACAACGCGAAGTTCTGATTTACTGAGAGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGAc                                                                         >  2:567348/1‑100 (MQ=255)
                           ccacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGAcc                                                                        >  1:788744/1‑99 (MQ=255)
                           ccacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGAAGGAGCGCCGAATGGATTAGCGAcc                                                                        >  2:957211/1‑99 (MQ=255)
                           ccacGTTATACAACGCGAAGTTCTGAATTACTGAGGGTCAAATAAATATAGCGACAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTCGCGa                                                                          >  2:957393/1‑97 (MQ=255)
                               gTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACa                                                                   >  2:2139857/1‑100 (MQ=255)
                                   taCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGa                                                                 >  2:408470/1‑98 (MQ=255)
                                   taCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATc                                                               >  2:406698/1‑100 (MQ=255)
                                      aaCGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACg                                                            >  2:650584/1‑100 (MQ=255)
                                      aaCGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACg                                                            >  2:1749083/1‑100 (MQ=255)
                                       aCGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCCGGAGAGCCGAATGGATTAGCGACCGAACAGATCACGt                                                           >  2:174074/1‑100 (MQ=255)
                                        cgcgAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGtt                                                          <  1:2520926/100‑1 (MQ=255)
                                          cgAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGGGCGCCGAATGGATTCGCGACCGAACAGATCACGtttt                                                        >  2:1726924/1‑100 (MQ=255)
                                           gAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGttttt                                                       <  1:261037/100‑1 (MQ=255)
                                             aGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTc                                                     <  1:861261/100‑1 (MQ=255)
                                              gTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCg                                                    <  1:1502802/100‑1 (MQ=255)
                                                tCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGc                                                   >  2:336638/1‑99 (MQ=255)
                                                   gATTTACTGAGGGTCCAATAAATATAGCGGCCGGAAAAAAGCGCTCCCGCAAGAGCGCCGACTGGATTAGCGACCGTACAGATCACGtctttcccc                                                   >  2:2185286/1‑92 (MQ=255)
                                                   gATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTa                                               <  1:2308052/100‑1 (MQ=255)
                                                      ttACTGAGAGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAAc                                            <  1:567355/100‑1 (MQ=255)
                                                         cTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGc                                         >  2:412419/1‑100 (MQ=255)
                                                             gggTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAAGAGATCACGTTTTTTCGCCTTAAACGGCTggg                                     <  1:1723892/100‑1 (MQ=255)
                                                             gggTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTggg                                     >  1:1410370/1‑100 (MQ=255)
                                                             gggTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCAGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTggg                                     <  2:1503534/100‑1 (MQ=255)
                                                              ggTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGa                                    >  1:2399744/1‑100 (MQ=255)
                                                               gTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGaa                                   <  2:846703/100‑1 (MQ=255)
                                                               gTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGaa                                   >  1:751303/1‑100 (MQ=255)
                                                                 cAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGaaa                                  <  2:1712305/99‑1 (MQ=255)
                                                                  aaataaatATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATc                                <  2:1025852/100‑1 (MQ=255)
                                                                     taaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATcacc                             <  1:1643442/100‑1 (MQ=255)
                                                                     taaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATcacc                             <  1:956774/100‑1 (MQ=255)
                                                                       aataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATcaccac                           <  2:961123/100‑1 (MQ=255)
                                                                       aataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTCGCTTTAAACGGCTGGGAAATCaccacc                          <  1:2217582/100‑1 (MQ=255)
                                                                         tataGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTTGCTTTAAATGGCTGCGAAAAcaccac                           >  2:1723775/1‑98 (MQ=255)
                                                                         tataGCGGCAGAAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCAccacca                         <  1:279404/100‑1 (MQ=255)
                                                                           taGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGc                       <  2:2055535/100‑1 (MQ=255)
                                                                             gCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCAACACCAGCAc                     >  2:1791611/1‑100 (MQ=255)
                                                                               ggCTGGAGAAAAGCGCTCCCGCATGAACGCCGAATGGATTAGCCACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCCGCACt                    >  2:1483854/1‑99 (MQ=255)
                                                                               ggCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACt                    >  2:1078501/1‑99 (MQ=255)
                                                                               ggCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTg                   >  2:1259385/1‑100 (MQ=255)
                                                                                  aaaaaaaaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGa                <  2:810681/97‑1 (MQ=255)
                                                                                  aGGAAAAAAGCGCTCCTGCACGAGCGCCGAATGGATTAGCGACCGAACAGATCAAGTTTTTTCGCGTTAAACGGCTCGGAAATCACCAACAGCACTGCGa                >  2:1674083/1‑100 (MQ=255)
                                                                                  aGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGa                >  1:2014775/1‑100 (MQ=255)
                                                                                   ggAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCAGTGCGAc               >  2:1877715/1‑100 (MQ=255)
                                                                                   ggAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGAc               <  1:464014/100‑1 (MQ=255)
                                                                                    gAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACa              >  2:277869/1‑100 (MQ=255)
                                                                                    gAAAAAAGCGCTCCCGCAGGAGAGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCTGCACTGCGa                >  2:1482625/1‑98 (MQ=255)
                                                                                      aaaaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAAt            >  1:2064170/1‑100 (MQ=255)
                                                                                      aaaaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAAt            >  1:39180/1‑100 (MQ=255)
                                                                                       aaaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATc           <  2:780920/100‑1 (MQ=255)
                                                                                        aaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCa          >  2:358552/1‑100 (MQ=255)
                                                                                        aaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCa          >  1:1966286/1‑100 (MQ=255)
                                                                                         cggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCAGGGTTTTGAGGTTTAAAGGGCTGGGGAAGCACCACGAGCACTGCggcgat            >  2:1362208/3‑92 (MQ=255)
                                                                                         cggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAg         >  1:1762439/3‑100 (MQ=255)
                                                                                         cggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAg         >  2:2397139/3‑100 (MQ=255)
                                                                                         aaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAg         <  2:2254561/100‑1 (MQ=255)
                                                                                         aaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAg         >  2:113047/1‑100 (MQ=255)
                                                                                          ggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAgg        >  2:1250611/2‑100 (MQ=255)
                                                                                          ggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAgg        >  2:113494/2‑100 (MQ=255)
                                                                                           gcgcTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGt       >  1:1362143/1‑100 (MQ=255)
                                                                                            cgcTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTa      >  1:354984/1‑100 (MQ=255)
                                                                                              cTCCCGCAGGAGCGCCGAATGGATTAGTGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAg    <  2:464809/100‑1 (MQ=255)
                                                                                              cTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTTTTTTTTTTTTTCTGGGAAATCACCACCAGCACTGCCACAATCAGGTAAg    >  2:2035160/1‑100 (MQ=255)
                                                                                               tCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGc   <  1:1167292/100‑1 (MQ=255)
                                                                                                cccGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGc   <  2:2398754/99‑1 (MQ=255)
                                                                                                cccGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCg  >  1:805474/1‑100 (MQ=255)
                                                                                                  |                                                                                                 
TGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCG  >  NC_000913/1232383‑1232578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: