Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,232,484 A→G 15.2% intergenic (+30/+16) umuC → / ← dsbB translesion error‑prone DNA polymerase V subunit; DNA polymerase activity/oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,232,4840AG15.2% 252.0 / 35.5 112intergenic (+30/+16)umuC/dsbBtranslesion error‑prone DNA polymerase V subunit; DNA polymerase activity/oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I
Reads supporting (aligned to +/- strand):  ref base A (58/36);  new base G (5/12);  total (64/48)
Fisher's exact test for biased strand distribution p-value = 1.72e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.80e-01

Only 100 of 113 total aligned reads displayed.

CAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAA  >  NC_000913/1232386‑1232583
                                                                                                  |                                                                                                   
cAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAg                                                                                                    >  1:670366/1‑100 (MQ=255)
cAGATAAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGTGGCAGGAAAAAAGCGCTCCCGCAg                                                                                                    <  2:135149/100‑1 (MQ=255)
 aGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAgg                                                                                                   <  1:2057294/100‑1 (MQ=255)
     gAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAgcg                                                                                               >  1:953185/1‑100 (MQ=255)
      aaGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAgcgc                                                                                              >  2:807974/1‑100 (MQ=255)
       aGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGcc                                                                                             >  2:1736828/1‑100 (MQ=255)
         cGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCCGGAAAAAAGCGCTCCCGCAGGAGCGCCGa                                                                                           >  2:86243/1‑100 (MQ=255)
          gAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgctttt                                                                                          <  2:1415018/100‑5 (MQ=255)
          gAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgctttt                                                                                          <  2:1308725/100‑5 (MQ=255)
           aGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttttt                                                                                         <  2:5906/100‑6 (MQ=255)
           aGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAAt                                                                                         >  1:1910367/1‑100 (MQ=255)
            gCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgctttttt                                                                                        <  1:1571869/100‑7 (MQ=255)
            gCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATg                                                                                        >  1:576853/1‑100 (MQ=255)
            gCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATg                                                                                        >  1:416543/1‑100 (MQ=255)
            gCCATGCGTTCACCACGTTATACGACTCGAAGTTCTGATTTACTTAGGGTCAAATAAATATTGCGGCAGGAAAAAAGCGCTCCTGCGGGAtcgctttt                                                                                          <  2:949728/98‑5 (MQ=255)
             ccATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATgg                                                                                       <  1:620857/100‑1 (MQ=255)
              cATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttttttcc                                                                                      <  1:2390945/100‑9 (MQ=38)
              cATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGa                                                                                      >  1:1933215/1‑100 (MQ=255)
              cATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAAGAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttttttcc                                                                                      <  2:499884/100‑9 (MQ=38)
               aTGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttttttcct                                                                                     <  2:92252/100‑10 (MQ=38)
               aTGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttttttcct                                                                                     <  2:221365/100‑10 (MQ=38)
                tGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttttttcct                                                                                     <  2:1718526/99‑10 (MQ=38)
                tGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGAtt                                                                                    <  2:1408052/100‑1 (MQ=255)
                 gCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGTCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTa                                                                                   >  2:1800770/1‑100 (MQ=255)
                 gCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTa                                                                                   >  2:19059/1‑100 (MQ=255)
                 gCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTa                                                                                   >  1:1975619/1‑100 (MQ=255)
                 gCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCCCCCGCAGGAGCGCCGAAt                                                                                         >  2:614903/1‑94 (MQ=255)
                 gCTTTCACCACGTTATACAACGCCACGTTCTGACATACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgctttttgcc                                                                                      <  2:1109788/97‑9 (MQ=255)
                  cTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAg                                                                                  <  1:38974/100‑1 (MQ=255)
                  cTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAg                                                                                  <  2:193714/100‑1 (MQ=255)
                  cTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAg                                                                                  >  2:2423838/1‑100 (MQ=255)
                   tgtCACCACGTTATACAACGCGCAGTTCTGATTTATGGAGCGCCAAATAAATATAGCGGCAGCAAAAAAGCGCTCCCGCATGAGCGCCGAATGGATTAGc                                                                                 <  2:64271/98‑1 (MQ=255)
                    ttCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCg                                                                                <  1:1614857/100‑1 (MQ=255)
                    ttCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCg                                                                                <  1:1655082/100‑1 (MQ=255)
                      caccacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGAc                                                                              >  2:1530722/1‑100 (MQ=255)
                      caccacGTTATACAACGCGAAGTTCTGATTTACTGAGAGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGAc                                                                              >  2:567348/1‑100 (MQ=255)
                        ccacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGAcc                                                                             >  1:788744/1‑99 (MQ=255)
                        ccacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGAAGGAGCGCCGAATGGATTAGCGAcc                                                                             >  2:957211/1‑99 (MQ=255)
                        ccacGTTATACAACGCGAAGTTCTGAATTACTGAGGGTCAAATAAATATAGCGACAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTCGCGa                                                                               >  2:957393/1‑97 (MQ=255)
                                taCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGa                                                                      >  2:408470/1‑98 (MQ=255)
                                taCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATc                                                                    >  2:2049691/1‑100 (MQ=255)
                                taCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATc                                                                    >  2:406698/1‑100 (MQ=255)
                                  cAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCAc                                                                  >  1:387736/1‑100 (MQ=255)
                                   aaCGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACg                                                                 >  2:650584/1‑100 (MQ=255)
                                    aCGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCCGGAGAGCCGAATGGATTAGCGACCGAACAGATCACGt                                                                >  2:174074/1‑100 (MQ=255)
                                     cgcgAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGtt                                                               <  1:2520926/100‑1 (MQ=255)
                                       cgAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGGGCGCCGAATGGATTCGCGACCGAACAGATCACGtttt                                                             >  2:1726924/1‑100 (MQ=255)
                                           gTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCg                                                         <  1:1502802/100‑1 (MQ=255)
                                             tCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGc                                                        >  2:336638/1‑99 (MQ=255)
                                                gATTTACTGAGGGTCCAATAAATATAGCGGCCGGAAAAAAGCGCTCCCGCAAGAGCGCCGACTGGATTAGCGACCGTACAGATCACGtctttcccc                                                        >  2:2185286/1‑92 (MQ=255)
                                                gATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTa                                                    <  1:2308052/100‑1 (MQ=255)
                                                  tttACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTaaa                                                  >  1:1750876/1‑100 (MQ=255)
                                                   ttACTGAGAGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAAc                                                 <  1:567355/100‑1 (MQ=255)
                                                     aCTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACgg                                               >  1:2094726/1‑100 (MQ=255)
                                                      cTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGc                                              >  2:412419/1‑100 (MQ=255)
                                                          gggTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAAGAGATCACGTTTTTTCGCCTTAAACGGCTggg                                          <  1:1723892/100‑1 (MQ=255)
                                                          gggTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTggg                                          >  1:1410370/1‑100 (MQ=255)
                                                           ggTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGa                                         >  1:2399744/1‑100 (MQ=255)
                                                            gTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGaa                                        <  2:846703/100‑1 (MQ=255)
                                                            gTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGaa                                        >  1:751303/1‑100 (MQ=255)
                                                              cAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGaaa                                       <  2:1712305/99‑1 (MQ=255)
                                                               aaataaatATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATc                                     <  2:1025852/100‑1 (MQ=255)
                                                                  taaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATcacc                                  <  1:956774/100‑1 (MQ=255)
                                                                    aataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATcaccac                                <  2:961123/100‑1 (MQ=255)
                                                                    aataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTCGCTTTAAACGGCTGGGAAATCaccacc                               <  1:2217582/100‑1 (MQ=255)
                                                                      tataGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTTGCTTTAAATGGCTGCGAAAAcaccac                                >  2:1723775/1‑98 (MQ=255)
                                                                      tataGCGGCAGAAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCAccacca                              <  1:279404/100‑1 (MQ=255)
                                                                        taGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGc                            <  2:2055535/100‑1 (MQ=255)
                                                                          gCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCAACACCAGCAc                          >  2:1791611/1‑100 (MQ=255)
                                                                            ggCTGGAGAAAAGCGCTCCCGCATGAACGCCGAATGGATTAGCCACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCCGCACt                         >  2:1483854/1‑99 (MQ=255)
                                                                            ggCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACt                         >  2:1078501/1‑99 (MQ=255)
                                                                               aaaaaaaaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGa                     <  2:810681/97‑1 (MQ=255)
                                                                               aGGAAAAAAGCGCTCCTGCACGAGCGCCGAATGGATTAGCGACCGAACAGATCAAGTTTTTTCGCGTTAAACGGCTCGGAAATCACCAACAGCACTGCGa                     >  2:1674083/1‑100 (MQ=255)
                                                                                ggAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCAGTGCGAc                    >  2:1877715/1‑100 (MQ=255)
                                                                                ggAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGAc                    <  1:464014/100‑1 (MQ=255)
                                                                                 gAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACa                   >  2:277869/1‑100 (MQ=255)
                                                                                 gAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCAACACCAGCACTGc                       >  2:2432935/1‑96 (MQ=255)
                                                                                 gAAAAAAGCGCTCCCGCAGGAGAGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCTGCACTGCGa                     >  2:1482625/1‑98 (MQ=255)
                                                                                   aaaaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAAt                 >  1:39180/1‑100 (MQ=255)
                                                                                   aaaaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAAt                 >  1:2064170/1‑100 (MQ=255)
                                                                                    aaaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATc                <  2:780920/100‑1 (MQ=255)
                                                                                    aaaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATc                >  1:691422/1‑100 (MQ=255)
                                                                                     aaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCa               >  2:358552/1‑100 (MQ=255)
                                                                                     aaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCa               >  1:1966286/1‑100 (MQ=255)
                                                                                      cggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCAGGGTTTTGAGGTTTAAAGGGCTGGGGAAGCACCACGAGCACTGCggcgat                 >  2:1362208/3‑92 (MQ=255)
                                                                                      cggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAg              >  1:1762439/3‑100 (MQ=255)
                                                                                      cggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAg              >  2:2397139/3‑100 (MQ=255)
                                                                                      aaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAg              <  2:2254561/100‑1 (MQ=255)
                                                                                      aaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAg              >  2:113047/1‑100 (MQ=255)
                                                                                       ggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAgg             >  2:113494/2‑100 (MQ=255)
                                                                                       ggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAgg             >  2:1250611/2‑100 (MQ=255)
                                                                                        gcgcTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGt            >  1:1362143/1‑100 (MQ=255)
                                                                                         cgcTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTa           >  1:354984/1‑100 (MQ=255)
                                                                                           cTCCCGCAGGAGCGCCGAATGGATTAGTGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAg         <  2:464809/100‑1 (MQ=255)
                                                                                           cTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTTTTTTTTTTTTTCTGGGAAATCACCACCAGCACTGCCACAATCAGGTAAg         >  2:2035160/1‑100 (MQ=255)
                                                                                            tCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGc        <  1:1167292/100‑1 (MQ=255)
                                                                                             cccGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGc        <  2:2398754/99‑1 (MQ=255)
                                                                                             cccGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCg       >  1:805474/1‑100 (MQ=255)
                                                                                                gCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATa    >  1:1027334/1‑100 (MQ=255)
                                                                                                  aGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATaaa  <  1:1200866/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
CAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAA  >  NC_000913/1232386‑1232583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: