Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,292,944 T→C 100% intergenic (‑22/‑583) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,292,9440TC99.2% 335.2 / ‑6.0 98intergenic (‑22/‑583)hns/tdkglobal DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (41/56);  total (42/56)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

CGTTTCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCC  >  NC_000913/1292848‑1293038
                                                                                                |                                                                                              
cGTTTCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTgg                                                                                             <  1:1959349/100‑1 (MQ=255)
   ttCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTgggg                                                                                           >  2:483771/1‑99 (MQ=255)
    tCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCACTGTAGTAATCTCAAACTTATATTGGggtg                                                                                         >  2:1538647/1‑100 (MQ=255)
     cAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGggtgg                                                                                        >  2:1240130/1‑100 (MQ=255)
     cAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGggtg                                                                                         >  2:963468/1‑99 (MQ=255)
      aaGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGggtggt                                                                                       <  2:2285522/100‑1 (MQ=255)
      aaGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGggtggt                                                                                       >  1:311368/1‑100 (MQ=255)
       aGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGtt                                                                                      >  1:1089575/1‑100 (MQ=255)
        gtgtACATTCTCTTGCCTGCGCACGACGAGTACGGCTGTTGTTCAGACTTTTAAGTGCTTCGCTCATTGTAGTAGTCTCAAACATATACTGGggt                                                                                          >  2:1710736/1‑95 (MQ=255)
        gtgtACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGttt                                                                                     >  2:2448276/1‑100 (MQ=255)
          gtACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTttgt                                                                                   <  2:2180749/100‑1 (MQ=255)
          gtACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTttgt                                                                                   <  1:299240/100‑1 (MQ=255)
          gtACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTttgt                                                                                   <  1:1465111/100‑1 (MQ=255)
            aCATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTttgttg                                                                                 <  2:376725/100‑1 (MQ=255)
              aTTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAg                                                                               <  2:2465468/100‑1 (MQ=255)
               ttCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAgg                                                                              <  2:1889463/100‑1 (MQ=255)
               ttCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAgg                                                                              <  1:1933474/100‑1 (MQ=255)
               ttCTCTTGCCTGCGCACGAAGAGTACGGATATTGTTCAGAATTTTAAGTGCTTCGCTGATTGTAGTAATCTCAACCTTATACTGGGGTGGTTTGTTGAgg                                                                              <  2:2342331/100‑1 (MQ=255)
                tctcTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGt                                                                             <  2:527319/100‑1 (MQ=255)
                  tctTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGtaa                                                                           >  2:1319617/1‑100 (MQ=255)
                  tctTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGtaa                                                                           >  2:823901/1‑100 (MQ=255)
                      gCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTaataat                                                                       <  2:1286322/100‑1 (MQ=255)
                      gCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTaataat                                                                       >  1:1531995/1‑100 (MQ=255)
                       ccTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAataata                                                                      <  2:1877729/100‑1 (MQ=255)
                       ccTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAataata                                                                      <  2:1815306/100‑1 (MQ=255)
                         tGCGCACGAAGATTACGGATGTTGTTCAGAATTTTAAGTGCGTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTaataat                                                                       <  2:1519190/97‑1 (MQ=255)
                         tGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATaga                                                                    >  1:956733/1‑100 (MQ=255)
                          gcgcACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATagtg                                                                   <  1:1727539/100‑3 (MQ=255)
                            gtaCGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGc                                                                  <  2:1759591/97‑1 (MQ=255)
                            gcACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAGACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGcc                                                                 >  2:1802660/1‑100 (MQ=255)
                            gcACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGcc                                                                 <  1:247431/100‑1 (MQ=255)
                             cACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCt                                                                >  2:840473/1‑100 (MQ=255)
                               cGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCtt                                                               >  1:2541098/1‑99 (MQ=255)
                               cGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTa                                                              <  2:1434782/100‑1 (MQ=255)
                                  agagTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAAt                                                           <  1:2204642/100‑1 (MQ=255)
                                    agTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTc                                                         <  1:910334/100‑1 (MQ=255)
                                    agTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTc                                                         >  1:1621083/1‑100 (MQ=255)
                                     gTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCa                                                        <  1:1685451/100‑1 (MQ=255)
                                     gTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCa                                                        <  1:1784612/100‑1 (MQ=255)
                                       aCGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAg                                                       <  1:1383470/99‑1 (MQ=255)
                                       aCGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGt                                                      <  1:482020/100‑1 (MQ=255)
                                       aCGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGt                                                      >  2:338794/1‑100 (MQ=255)
                                        cGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGtt                                                     >  1:82835/1‑100 (MQ=255)
                                        cGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGtt                                                     >  1:985652/1‑100 (MQ=255)
                                        cGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGtt                                                     <  2:1041536/100‑1 (MQ=255)
                                         ggATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTtg                                                    <  2:1737507/100‑1 (MQ=255)
                                            tgttgtTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGc                                                 <  2:984098/100‑1 (MQ=255)
                                            tgttgtTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGc                                                 <  2:780562/100‑1 (MQ=255)
                                            tgttgtTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGc                                                 <  1:2448290/100‑1 (MQ=255)
                                            tgttgtTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGc                                                 <  1:2224392/100‑1 (MQ=255)
                                             gttgttCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCa                                                <  2:600397/100‑1 (MQ=255)
                                             gttgtgGAGGATGTTAAGTGGTTGGCTAATTGTAGTAATTTCAAACTTATCCTGGGGTGGTTTGTTGTGGTAATAATAGAGCCTTAAATTCAGTTGTGc                                                 <  2:2127069/99‑1 (MQ=255)
                                                gttCAGAATTTTAAGTGCTTCGCTCATTGTAGTTATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAAGAATAGAGCCTTAAATTCAGTTGTGCAATa                                             >  2:1539535/1‑100 (MQ=255)
                                                gttCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCa                                                >  2:2473865/1‑97 (MQ=255)
                                                gttCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATa                                             <  2:1073071/100‑1 (MQ=255)
                                                gttCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATa                                             >  2:774546/1‑100 (MQ=255)
                                                  tCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGc                                           >  1:510067/1‑100 (MQ=255)
                                                   cAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGcc                                          >  2:2069345/1‑100 (MQ=255)
                                                   cAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGcc                                          <  1:1702900/100‑1 (MQ=255)
                                                   cAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGc                                           >  2:1044742/1‑99 (MQ=255)
                                                    aGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCa                                         >  2:2414303/1‑100 (MQ=255)
                                                    aGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCa                                         >  1:776707/1‑100 (MQ=255)
                                                     gAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAg                                        >  1:77798/1‑100 (MQ=255)
                                                     gAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAg                                        >  1:696368/1‑100 (MQ=255)
                                                      aaTTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAgg                                       <  2:1601645/100‑1 (MQ=255)
                                                       aTTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGa                                      <  2:600705/100‑1 (MQ=255)
                                                       aTTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGa                                      >  1:1576366/1‑100 (MQ=255)
                                                          ttAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATg                                   <  1:1639975/100‑1 (MQ=255)
                                                            aaGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTa                                 <  1:2325331/100‑1 (MQ=255)
                                                             aGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTaa                                <  1:1048472/100‑1 (MQ=255)
                                                              gTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGCGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAg                               >  2:59338/1‑100 (MQ=255)
                                                              gTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGAGTGGTTTGTTGAGGAAATAATATAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAg                               <  2:1531683/100‑1 (MQ=255)
                                                               tGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAgn                              <  2:2277719/100‑2 (MQ=255)
                                                               tGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAgg                              <  2:2093799/100‑1 (MQ=255)
                                                                 cTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGaa                            <  2:393517/100‑1 (MQ=255)
                                                                  ttCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAAt                           >  1:2497032/1‑100 (MQ=255)
                                                                   tggCTTATTGTAGTAATTTAAAACTTATACTTGGTTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAgg                              <  2:1413401/94‑1 (MQ=255)
                                                                    cGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTc                         >  2:995804/1‑100 (MQ=255)
                                                                     gCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCa                        >  1:2350960/1‑100 (MQ=255)
                                                                      cTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCaa                       <  2:804817/100‑1 (MQ=255)
                                                                      cTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTGGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCaa                       >  2:1367065/1‑100 (MQ=255)
                                                                       tCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAACAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCaaa                      <  1:266625/100‑1 (MQ=255)
                                                                        cATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCaaaa                     >  2:1776410/1‑100 (MQ=255)
                                                                         aTTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAAt                    <  1:557972/100‑1 (MQ=255)
                                                                              agtaATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTc               >  2:1936871/1‑100 (MQ=255)
                                                                              agtaATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTc               <  2:1767775/100‑1 (MQ=255)
                                                                                taATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGTAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCtt             <  1:1802674/100‑1 (MQ=255)
                                                                                 aaTCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCttt            <  2:836970/100‑1 (MQ=255)
                                                                                 aaTCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCttt            >  2:149870/1‑100 (MQ=255)
                                                                                    ctcAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAtt         >  1:1418266/1‑100 (MQ=255)
                                                                                     tcAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGGAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAttt        >  2:2413375/1‑100 (MQ=255)
                                                                                      cAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAtttt       >  2:832976/1‑100 (MQ=255)
                                                                                      cAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAtttt       >  2:1724549/1‑100 (MQ=255)
                                                                                      cAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAtttt       <  1:230344/100‑1 (MQ=255)
                                                                                       aaaCTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGATTTCAAAATTGTTCTTTATTTtg      <  2:1485323/100‑1 (MQ=255)
                                                                                       aaaCTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTtg      <  2:209377/100‑1 (MQ=255)
                                                                                          cTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTgc   <  2:1267943/100‑1 (MQ=255)
                                                                                           ttATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTgcc  <  1:1493968/100‑1 (MQ=255)
                                                                                                |                                                                                              
CGTTTCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCC  >  NC_000913/1292848‑1293038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: