Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,500,814 Δ1 bp intergenic (‑23/‑446) yjgX ← / → yjgZ pseudogene fragment/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,500,8110T.99.0% 468.9 / ‑2.5 104intergenic (‑20/‑449)yjgX/yjgZpseudogene fragment/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base . (47/56);  total (47/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 117 total aligned reads displayed.

GTCCGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAGGGGG  >  NC_000913/4500712‑4500906
                                                                                                   |                                                                                               
gtCCGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAgtgt                                                                                                 <  2:656097/100‑1 (MQ=255)
gtCCGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAgtgt                                                                                                 <  1:61869/100‑1 (MQ=255)
gtCCGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAgtgt                                                                                                 >  2:2243147/1‑100 (MQ=255)
 tCCGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtt                                                                                                <  2:2623754/100‑1 (MQ=255)
 tCCGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtt                                                                                                <  2:1070457/100‑1 (MQ=255)
  ccGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGttt                                                                                               >  2:1317610/1‑100 (MQ=255)
   cGCCAGAAAATCACCACGAATACCGGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtttc                                                                                              <  1:607972/100‑2 (MQ=255)
   cGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtttc                                                                                              <  1:187105/100‑2 (MQ=255)
   cGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtttc                                                                                              >  2:1606924/1‑99 (MQ=255)
    gCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtttct                                                                                             >  1:2452474/1‑98 (MQ=255)
    gCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtttct                                                                                             >  1:1849604/1‑98 (MQ=255)
      cAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtttctgt                                                                                           >  2:3243467/1‑96 (MQ=255)
       aGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGCAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTa                                                                                         <  1:2028236/100‑1 (MQ=255)
       aGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTa                                                                                         <  1:473906/100‑1 (MQ=255)
       aGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTAACTTTGCTTTCTCCTGTAGTGTTTCTGTa                                                                                         >  2:2028265/1‑100 (MQ=255)
        gAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAg                                                                                        <  2:1550377/100‑1 (MQ=255)
            aTCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTc                                                                                    >  2:584954/1‑100 (MQ=255)
             tCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCt                                                                                   <  2:2915947/100‑1 (MQ=255)
             tCACCACGAATACCAGAAGCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCt                                                                                   <  1:747483/100‑1 (MQ=255)
              cactacGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCTGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTg                                                                                  <  1:985551/100‑1 (MQ=255)
              caccacGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTg                                                                                  <  1:805465/100‑1 (MQ=255)
              caccacGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGGTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTg                                                                                  >  2:3390030/1‑100 (MQ=255)
               accacGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGa                                                                                 <  1:825087/100‑1 (MQ=255)
               accacGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGa                                                                                 >  2:199970/1‑100 (MQ=255)
                  acGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAAcc                                                                              <  2:2681768/100‑1 (MQ=255)
                  acGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAAcc                                                                              <  2:1990140/100‑1 (MQ=255)
                  acGAAAACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGAGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAAcc                                                                              <  1:3121862/100‑1 (MQ=255)
                   cGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCg                                                                             >  2:875624/1‑100 (MQ=255)
                    gAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCgg                                                                            <  2:2008972/100‑1 (MQ=255)
                     aaTACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGt                                                                           <  2:1287081/100‑1 (MQ=255)
                      aTACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGtt                                                                          >  2:3084222/1‑100 (MQ=255)
                         ccAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTa                                                                       >  1:1145372/1‑100 (MQ=255)
                         ccAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTa                                                                       >  1:3230832/1‑100 (MQ=255)
                         ccAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTa                                                                       >  1:2609953/1‑100 (MQ=255)
                          cAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTtata                                                                       >  1:541297/1‑99 (MQ=255)
                          cAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAg                                                                      >  2:2073362/1‑100 (MQ=255)
                            gAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCg                                                                    >  2:1623970/1‑100 (MQ=255)
                             aaTCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCgg                                                                   <  2:2690980/100‑1 (MQ=255)
                              aTCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCggg                                                                  <  2:3363240/100‑1 (MQ=255)
                               tCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCgggg                                                                 <  2:2538828/100‑1 (MQ=255)
                               tCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCgggg                                                                 <  2:3097878/100‑1 (MQ=255)
                                cgcgGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGt                                                                <  1:63904/100‑1 (MQ=255)
                                cgcgGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGt                                                                <  1:1652831/100‑1 (MQ=255)
                                    gCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTtgtg                                                            >  1:641467/1‑100 (MQ=255)
                                      aCTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTtgtg                                                            <  1:1446459/99‑1 (MQ=255)
                                      aCTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAg                                                          >  1:2926674/1‑100 (MQ=255)
                                      aCTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAg                                                          <  2:1930584/100‑1 (MQ=255)
                                       cTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAgg                                                         <  2:2271612/100‑1 (MQ=255)
                                         gACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCt                                                       >  1:2215710/1‑100 (MQ=255)
                                           ccAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGa                                                     >  1:2314895/1‑100 (MQ=255)
                                             aGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATg                                                   <  2:3090224/100‑1 (MQ=255)
                                             aGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATg                                                   >  2:1551329/1‑100 (MQ=255)
                                             aGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATg                                                   >  1:1278991/1‑100 (MQ=255)
                                              gAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATgg                                                  >  2:1198988/1‑100 (MQ=255)
                                               aaaaTGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGc                                                 <  1:3423983/100‑1 (MQ=255)
                                               aaaaTGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGc                                                 <  2:759738/100‑1 (MQ=255)
                                                   tGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCACTc                                             >  1:3213849/1‑100 (MQ=255)
                                                      tAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGcc                                          >  1:1754292/1‑100 (MQ=255)
                                                      tAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGcc                                          >  2:3398257/1‑100 (MQ=255)
                                                        aaCAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCgg                                        >  2:1368255/1‑100 (MQ=255)
                                                        aaCAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCgg                                        >  1:2341167/1‑100 (MQ=255)
                                                        aaCAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCgg                                        <  2:3212560/100‑1 (MQ=255)
                                                         aCAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCggg                                       <  2:2219869/100‑1 (MQ=255)
                                                         aCAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCggg                                       >  1:145226/1‑100 (MQ=255)
                                                          cAGGCAACACGGGGCGGTTCACTTTGATTTCTCCTGTCGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCg                                         >  1:146803/1‑97 (MQ=255)
                                                          cAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCggn                                       <  1:255683/99‑2 (MQ=255)
                                                          cAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGc                                      <  2:1818026/100‑1 (MQ=255)
                                                          cAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGc                                      >  1:575624/1‑100 (MQ=255)
                                                             gAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGCTTCTGTAGGTTCTGAACCGGTTCTTGCGGGTCTGTGAGGCTGATGGCAATTGCATGGct                                     >  1:1649809/1‑98 (MQ=255)
                                                               aaCACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTc                                 >  2:1097120/1‑100 (MQ=255)
                                                                acacGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTcc                                <  2:1279008/100‑1 (MQ=255)
                                                                acacGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTcc                                <  2:2164070/100‑1 (MQ=255)
                                                                acacGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTcc                                <  2:2215742/100‑1 (MQ=255)
                                                                 cacGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCt                               <  1:2682201/100‑1 (MQ=255)
                                                                 cacGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCt                               >  1:687097/1‑100 (MQ=255)
                                                                  acGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTg                              <  1:3398205/100‑1 (MQ=255)
                                                                     gggCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCAt                           >  2:1403785/1‑100 (MQ=255)
                                                                      ggCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATg                          <  2:2349428/100‑1 (MQ=255)
                                                                         ggTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTa                       <  1:2090610/100‑1 (MQ=255)
                                                                          gTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTac                      >  2:1485654/1‑100 (MQ=255)
                                                                          gTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTac                      >  2:3028326/1‑100 (MQ=255)
                                                                             cACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACt                   <  1:532697/100‑1 (MQ=255)
                                                                               cTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTgg                 >  2:2982942/1‑100 (MQ=255)
                                                                                   gCTTTCTCCTGTAGTGTGTCGGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTAc              <  2:3224435/99‑1 (MQ=255)
                                                                                    cTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACtn            <  1:901921/100‑2 (MQ=255)
                                                                                    cTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTg            <  2:1472584/100‑1 (MQ=255)
                                                                                    cTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTg            >  1:466194/1‑100 (MQ=255)
                                                                                    cTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTg            <  1:2718986/100‑1 (MQ=255)
                                                                                    cTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTGCCTGCATGTTACACTGGTACTg            >  1:716973/1‑100 (MQ=255)
                                                                                     tttCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGc           <  2:687106/100‑1 (MQ=255)
                                                                                      ttCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCCATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCa          <  1:2137150/100‑1 (MQ=255)
                                                                                      ttCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCa          <  2:2846175/100‑1 (MQ=255)
                                                                                       tctcCTGTCGTGTTTCTGTAGGTTCCGACCCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCa          <  2:156300/99‑1 (MQ=255)
                                                                                       tctcCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCaa         >  1:260286/1‑100 (MQ=255)
                                                                                          ccTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAagag      >  2:1367621/1‑100 (MQ=255)
                                                                                          ccTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAagag      >  2:3023848/1‑100 (MQ=255)
                                                                                           cTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGGTCTTCCTGCATGTTACACTCGTACTGCAcgag      >  1:2887173/1‑99 (MQ=255)
                                                                                             gTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAgggg   >  1:1712589/1‑100 (MQ=255)
                                                                                             gTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAgggg   >  1:2356148/1‑100 (MQ=255)
                                                                                              tAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAggggg  <  1:3024069/100‑1 (MQ=255)
                                                                                                   |                                                                                               
GTCCGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAGGGGG  >  NC_000913/4500712‑4500906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: