Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 107,553 (T)9→8 intergenic (+79/‑152) lpxC → / → secM UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913107,5450T.96.1% 431.8 / 9.0 103intergenic (+71/‑160)lpxC/secMUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base . (44/55);  total (47/56)
Fisher's exact test for biased strand distribution p-value = 3.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

CAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGA  >  NC_000913/107447‑107639
                                                                                                  |                                                                                              
cAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGtt                                                                                               >  2:3456628/1‑100 (MQ=255)
   aGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGGATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttt                                                                                            <  1:2878655/100‑1 (MQ=255)
     cgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGTCAGGTGAACCAGTCGttttttt                                                                                          <  1:2019337/100‑1 (MQ=255)
     cgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttt                                                                                          <  2:1893440/100‑1 (MQ=255)
     cgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttt                                                                                          <  1:1485322/100‑1 (MQ=255)
      gcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGtttttttt                                                                                         <  2:2230596/100‑1 (MQ=255)
         ttCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttgaa                                                                                      >  2:823046/1‑97 (MQ=255)
          tCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAATATTCTACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttgaa                                                                                      <  2:3448893/99‑4 (MQ=255)
          tCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttgaa                                                                                      <  2:1569371/99‑4 (MQ=255)
             gCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAAtttt                                                                                 >  1:1803339/1‑100 (MQ=255)
              cTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTta                                                                                >  1:1334810/1‑100 (MQ=255)
               tGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTtat                                                                               <  1:596569/100‑1 (MQ=255)
                gTACTGGCATAACTACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTtata                                                                              <  2:2025310/100‑1 (MQ=255)
                gTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTtata                                                                              <  1:151974/100‑1 (MQ=255)
                  aCTGGCATAACGACATTTATACTGTCATATAAAATGCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTtat                                                                               <  1:1560930/97‑1 (MQ=255)
                   cTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAaga                                                                           <  2:3258139/100‑1 (MQ=255)
                   cTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAaga                                                                           <  2:2821618/100‑1 (MQ=255)
                     ggCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGc                                                                         >  2:2098926/1‑100 (MQ=255)
                       cATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCta                                                                       >  2:797832/1‑100 (MQ=255)
                       cATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCta                                                                       >  2:1425998/1‑100 (MQ=255)
                        aTAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGGCTTTTTTTTTGAGTTTTATAAGAGCtat                                                                      >  2:1378152/1‑100 (MQ=255)
                         tatcGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGTCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCtata                                                                     <  1:1299842/97‑1 (MQ=255)
                          aaCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCtata                                                                     >  1:1641739/1‑99 (MQ=255)
                           aCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCtatata                                                                   <  2:2344619/100‑3 (MQ=255)
                            cGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATaaaa                                                                  >  2:2104645/1‑100 (MQ=255)
                               cATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAAc                                                               >  2:267251/1‑100 (MQ=255)
                                 tttATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACgg                                                             >  2:2645819/1‑100 (MQ=255)
                                 tttATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACgg                                                             <  1:555930/100‑1 (MQ=255)
                                  ttATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGt                                                            <  2:2608634/100‑1 (MQ=255)
                                  ttATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGt                                                            <  2:2608500/100‑1 (MQ=255)
                                   tataCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTg                                                           >  2:121320/1‑100 (MQ=255)
                                    ataCTGTCGTATAAAATTCGACTGGCAAATCTGGCACGCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGc                                                          >  1:1861062/1‑100 (MQ=255)
                                          tCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGc                                                    <  1:91763/100‑1 (MQ=255)
                                            gTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTg                                                  >  2:805790/1‑100 (MQ=255)
                                             tataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGt                                                 <  1:238158/100‑1 (MQ=255)
                                             tataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGt                                                 >  1:178912/1‑100 (MQ=255)
                                              ataAAATTCGACTGGCAAATCTGGCACTCTCTCCGACCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGtt                                                >  2:1743962/1‑100 (MQ=255)
                                               taAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGttt                                               >  1:1124000/1‑100 (MQ=255)
                                               taAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACACTGttt                                               >  2:1945001/1‑100 (MQ=255)
                                                aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTGTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGtttt                                              >  1:1027087/1‑100 (MQ=255)
                                                 cgatctgACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTc                                             <  2:1181563/94‑1 (MQ=255)
                                                 aaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTc                                             >  1:2687622/1‑100 (MQ=255)
                                                 aaaTGCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTc                                             <  2:2080312/100‑1 (MQ=255)
                                                  aaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCt                                            <  2:1778520/100‑1 (MQ=255)
                                                   aTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCtt                                           <  1:3137021/100‑1 (MQ=255)
                                                   aTTCGACTGGCAAATCTGACACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACgagagcggaag                                           >  2:1514122/1‑90 (MQ=255)
                                                    ttCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCtt                                           <  1:3137013/99‑1 (MQ=255)
                                                    ttCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTa                                          <  1:1328475/100‑1 (MQ=255)
                                                    ttCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTa                                          <  1:2352766/100‑1 (MQ=255)
                                                      cGACTGGCAAATCTGGCCCTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                        <  1:3052553/100‑1 (MQ=255)
                                                      cGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                        <  2:253989/100‑1 (MQ=255)
                                                      cGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                        <  2:3199862/100‑1 (MQ=255)
                                                      cGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTACGAACGCTGTTTTCTTAAg                                        >  1:423269/1‑100 (MQ=255)
                                                       gACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTcagatcg                                       >  1:1181548/1‑94 (MQ=255)
                                                         cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCAc                                     <  2:1835153/100‑1 (MQ=255)
                                                           ggCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACtt                                   >  2:1999003/1‑100 (MQ=255)
                                                              aaaTCTGGCACTCTCTCCAGCCAGGTGAACAAGTAGTTTTTTTTGAATTTTATAAGAGCTAGAAAAGACGGTGCGAACGGTGTTTTCTTAAGCACt                                    >  1:659395/1‑96 (MQ=255)
                                                               aaTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTcc                               >  2:2987748/1‑100 (MQ=255)
                                                                aTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTTTTTTTTTTAGAGCTATAAAAAACGGTCCATACGCTGTTTTCTTAAGCACTTTTcc                               >  1:1960957/1‑99 (MQ=255)
                                                                 tcttgtaCTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTAAAAAAAAAAAAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGc                             <  1:3320012/94‑1 (MQ=255)
                                                                 tCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGc                             <  2:3129934/100‑1 (MQ=255)
                                                                 tCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGc                             <  1:2089096/100‑1 (MQ=255)
                                                                 tCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGc                             <  2:1059736/100‑1 (MQ=255)
                                                                 tCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGc                             <  1:352405/100‑1 (MQ=255)
                                                                  cTGGCACTCTCCCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGca                            <  1:2498313/100‑1 (MQ=255)
                                                                    ggCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGcaca                          <  2:2310118/100‑1 (MQ=255)
                                                                    ggCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGcaca                          >  2:3389076/1‑100 (MQ=255)
                                                                    ggCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGcaca                          <  1:79062/100‑1 (MQ=255)
                                                                    ggCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGcaca                          >  2:3394574/1‑100 (MQ=255)
                                                                     gCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACaa                         >  2:1826177/1‑100 (MQ=255)
                                                                      cACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAAc                        <  1:2521331/100‑1 (MQ=255)
                                                                      cACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAAc                        <  2:1641761/100‑1 (MQ=255)
                                                                      cACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAAc                        <  2:2101294/100‑1 (MQ=255)
                                                                      cACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAAc                        <  1:3394522/100‑1 (MQ=255)
                                                                       aCTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACt                       >  2:699600/1‑100 (MQ=255)
                                                                       aCTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACt                       >  2:415558/1‑100 (MQ=255)
                                                                        ctctctCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACtt                      <  1:1713983/100‑1 (MQ=255)
                                                                            ctcCGGCCAGGTGAACCAGTCGTTTTTTTTTTTTTTTTTAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCt                  >  1:1024647/1‑100 (MQ=255)
                                                                            ctcCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATc                   >  1:2795525/1‑99 (MQ=255)
                                                                             tcCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCtt                 <  2:907746/100‑1 (MQ=255)
                                                                               cGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCa               >  1:2867601/1‑100 (MQ=255)
                                                                               cGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCa               <  2:1074691/100‑1 (MQ=255)
                                                                                ggCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCAt              >  1:180899/1‑100 (MQ=255)
                                                                                ggCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCAt              >  2:2914497/1‑100 (MQ=255)
                                                                                 gCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCAtt             <  2:1223419/100‑1 (MQ=255)
                                                                                 gCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCAtt             <  1:823035/100‑1 (MQ=255)
                                                                                  ccATGTGAACCAGTCGTTTTTTTTTAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCa               <  1:886533/97‑1 (MQ=255)
                                                                                   cAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTTTTAAGCACTTTTCCGCACAACTTATCTTCATTCg           >  1:436883/1‑100 (MQ=255)
                                                                                    aGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGt          >  1:438145/1‑100 (MQ=255)
                                                                                       tGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCt       >  2:2038414/1‑100 (MQ=255)
                                                                                       tGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCt       <  2:892235/100‑1 (MQ=255)
                                                                                         aaCCAGTCGTTTTTTTTGAATTTTATAATAGCTATAAAAAACGGTTCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtg      >  1:2660214/1‑99 (MQ=255)
                                                                                         aaCCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCttt     >  1:2061872/1‑98 (MQ=255)
                                                                                          aCCAGTCGTTTTTTTTGAATTTTATAATAGTTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgtg    <  1:2641831/100‑1 (MQ=255)
                                                                                          aCCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgtg    <  1:473281/100‑1 (MQ=255)
                                                                                          aCCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgtg    >  2:1529304/1‑100 (MQ=255)
                                                                                           ccAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAGGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTgg   <  1:38560/100‑1 (MQ=255)
                                                                                           ccAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTgg   <  2:764941/100‑1 (MQ=255)
                                                                                           ccAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTgg   >  2:1867873/1‑100 (MQ=255)
                                                                                             aGTCGTTTTTTTTGAATTTTATAAGAGCTCTAAAAAACGGTTCGAATGCTGTTTTCTTATGCACTTTTCAGCAAAACTCATCTTCATTCGTGCAGTTGa  >  1:2340336/1‑99 (MQ=255)
                                                                                                  |                                                                                              
CAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGA  >  NC_000913/107447‑107639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: