Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,170,338 (A)6→5 coding (37/963 nt) ldtC ← L,D‑transpeptidase linking Lpp to murein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,170,3330A.97.3% 636.6 / 8.1 147coding (42/963 nt)ldtCL,D‑transpeptidase linking Lpp to murein
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base . (80/63);  total (81/66)
Fisher's exact test for biased strand distribution p-value = 3.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

GAAATTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTATGG  >  NC_000913/1170236‑1170431
                                                                                                 |                                                                                                  
gAAATTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTaaa                                                                                                  >  1:2837519/1‑100 (MQ=255)
 aaaTTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTaaaa                                                                                                 <  2:237656/100‑1 (MQ=255)
    tttGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTaaaaacg                                                                                              <  2:1842396/100‑3 (MQ=255)
    tttGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTaaaaacg                                                                                              <  2:227131/100‑3 (MQ=255)
       gTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTa                                                                                          <  2:280613/100‑1 (MQ=255)
         tttCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGc                                                                                        >  2:1281565/1‑100 (MQ=255)
          ttCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGcc                                                                                       <  2:1993627/100‑1 (MQ=255)
          ttCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGcc                                                                                       <  2:1902744/100‑1 (MQ=255)
          ttCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGcc                                                                                       <  2:1993661/100‑1 (MQ=255)
            cGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGcc                                                                                       <  1:2885895/98‑1 (MQ=255)
            cGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAg                                                                                     >  1:3011432/1‑100 (MQ=255)
             gCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGc                                                                                    <  2:905986/100‑1 (MQ=255)
              ccAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCg                                                                                   >  2:462662/1‑100 (MQ=255)
               cAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCga                                                                                  >  2:1360224/1‑100 (MQ=255)
               cAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCga                                                                                  >  1:1668440/1‑100 (MQ=255)
               cAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCga                                                                                  <  2:2559586/100‑1 (MQ=255)
                aaCCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCgag                                                                                 <  2:3180686/100‑1 (MQ=255)
                 aCCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCgaga                                                                                <  2:564166/100‑1 (MQ=255)
                       cgacTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAAcg                                                                          >  2:1623865/1‑100 (MQ=255)
                       cgacTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAAcg                                                                          <  1:3160754/100‑1 (MQ=255)
                         ccTGGCCGCTGCCGCCAGCGGGCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAAcgcg                                                                        >  1:2487511/2‑100 (MQ=255)
                         ccTGGCCGCTGCCGCCAGCGGGCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAAcgcg                                                                        >  1:305791/2‑100 (MQ=255)
                         acTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAAcgcg                                                                        >  2:2179958/1‑100 (MQ=255)
                         acTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTGGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAAcgcg                                                                        >  2:2455652/1‑100 (MQ=255)
                          cTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAAcgcg                                                                        >  1:488456/1‑99 (MQ=255)
                           tggccGCTGCCGCCAGCGGGCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCCAGAAAAACGCGtt                                                                      >  1:2395103/4‑100 (MQ=255)
                            ggccGCTGCCGCCAGCGGGCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGttt                                                                     >  1:877110/3‑100 (MQ=255)
                            gCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGttt                                                                     >  2:1585699/1‑100 (MQ=255)
                            gCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGttt                                                                     >  1:2406611/1‑100 (MQ=255)
                            gCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGttt                                                                     >  2:3179701/1‑100 (MQ=255)
                             cccGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGtttt                                                                    <  2:1472713/100‑1 (MQ=255)
                             cccGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGtttt                                                                    >  2:1706116/1‑100 (MQ=255)
                                 cTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGatc                                                                <  1:1315185/100‑1 (MQ=255)
                                 cTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGatc                                                                <  1:3337023/100‑1 (MQ=255)
                                  tGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGatca                                                               <  2:2408916/100‑1 (MQ=255)
                                   ggcggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGatcat                                                              >  2:532474/1‑100 (MQ=255)
                                   ggcggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGatcat                                                              <  2:1653897/100‑1 (MQ=255)
                                   ggcggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGatcat                                                              >  1:3246990/1‑100 (MQ=255)
                                    gcggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGatcatc                                                             <  2:785841/100‑1 (MQ=255)
                                    gcggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGatcatc                                                             >  2:2293436/1‑100 (MQ=255)
                                     cggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGAtcatca                                                            >  2:1444583/1‑100 (MQ=255)
                                     cggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGAtcatca                                                            <  1:268851/100‑1 (MQ=255)
                                     cggcAGCGGCCAGGTGTTGGCTTTTGCCGATAGCGCCAGCGCCACTGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGAtcatca                                                            >  1:2846735/1‑100 (MQ=255)
                                      ggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCAc                                                           >  1:246669/1‑100 (MQ=255)
                                       gcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCAcc                                                          <  1:1551264/100‑1 (MQ=255)
                                        cAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCAAGGCAGCGGCGAACGTAAAACCGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCa                                                         >  1:1902498/1‑100 (MQ=255)
                                           cGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCaaaa                                                      >  2:426243/1‑100 (MQ=255)
                                           cGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCaaaa                                                      >  2:1084846/1‑100 (MQ=255)
                                            ggCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCaaa                                                       >  1:232846/1‑98 (MQ=255)
                                             gCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAAt                                                    >  1:2656428/1‑100 (MQ=255)
                                              ccAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATc                                                   <  1:1792930/100‑1 (MQ=255)
                                               cAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATcc                                                  <  1:1933386/100‑1 (MQ=255)
                                                aGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCa                                                 <  1:3097480/100‑1 (MQ=255)
                                                 ggTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCat                                                <  1:1149178/100‑1 (MQ=255)
                                                    gtTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCataat                                             >  2:1227823/1‑100 (MQ=255)
                                                    gtTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCataat                                             <  1:2607311/100‑1 (MQ=255)
                                                    gtTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCataat                                             >  2:1281759/1‑100 (MQ=255)
                                                     ttGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCataata                                            <  1:1331596/100‑1 (MQ=255)
                                                      tGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCAtaataa                                           >  2:1222879/1‑100 (MQ=255)
                                                       ggCTGTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATaataaa                                          <  1:2876338/100‑1 (MQ=255)
                                                         cTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATaataaata                                        >  1:2148459/1‑100 (MQ=255)
                                                          ttttGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATaataaata                                        <  2:2956205/100‑1 (MQ=255)
                                                           tttGCCCGTAGCGCCAGCGCCACAACAGCGGCGAACTTAAAAACATTAAAAAAAAAAAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAatat                                       <  1:448977/99‑1 (MQ=255)
                                                            ttGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATaa                                     <  1:2586928/100‑1 (MQ=255)
                                                             tGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAg                                    <  1:2766562/100‑1 (MQ=255)
                                                              gCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAgg                                   >  2:1416524/1‑100 (MQ=255)
                                                              gCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAgg                                   >  2:1347825/1‑100 (MQ=255)
                                                              gCCGGTAGCGCCAGCGCCACGGCAGCGGCGAAAGTAAAAACGTTAGCCAGCGAGAAAAACGCGTATAGATCATCACCAAAAATCCATAAAAAATATaa                                     >  1:3013361/1‑98 (MQ=255)
                                                               ccGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGt                                  >  1:835516/1‑100 (MQ=255)
                                                               ccGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGt                                  >  2:259017/1‑100 (MQ=255)
                                                                  gTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAACACGTTAGCCAGCGAGAAAAACGCGCTTTGATCATAACCAAAAATCCATAATAAATATAAgtgta                                >  1:3220777/1‑95 (MQ=255)
                                                                  gTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTa                                >  1:1720397/1‑99 (MQ=255)
                                                                    agcgccagcgccACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTAttn                             <  1:2869102/100‑2 (MQ=255)
                                                                    agcgccagcgccACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTg                             <  2:2031230/100‑1 (MQ=255)
                                                                    agcgccagcgccACGGCAGCGGCGAACGTAAAAACGTTAACCAGCGAGAAAAACGTGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTg                             <  1:561107/100‑1 (MQ=255)
                                                                     gcgccagcgccaCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGt                            >  1:2643462/1‑100 (MQ=255)
                                                                      cgccagcgccaCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGta                           >  2:3339873/1‑100 (MQ=255)
                                                                       gccagcgccaCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGtaa                          >  1:3304141/1‑100 (MQ=255)
                                                                        ccagcgccaCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGtaat                         >  1:1807678/1‑100 (MQ=255)
                                                                          agcgccaCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCTAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGtaataa                       <  1:220517/100‑1 (MQ=255)
                                                                          agcgccaCGGCAGCGGCAAACGTAAAAACGTTAGCCAGAGAAAAAAACGCAAAATAATAATGACCAAAATTCCATAATGCGTATAAGTTGATTGt                            <  1:639164/95‑1 (MQ=255)
                                                                            cgccaCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAAc                     >  2:2781467/1‑100 (MQ=255)
                                                                            cgccaCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAAc                     >  1:777281/1‑100 (MQ=255)
                                                                            cgccaCGGCAGCGGCGAACGTAAAAACGTTAGACAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAAc                     <  1:3367134/100‑1 (MQ=255)
                                                                             gccaCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAAc                     <  2:402657/99‑1 (MQ=255)
                                                                             gccaCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACa                    >  2:814821/1‑100 (MQ=255)
                                                                              ccaCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAg                   <  2:2893673/100‑1 (MQ=255)
                                                                              ccaCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAg                   >  1:411160/1‑100 (MQ=255)
                                                                               caCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGa                  <  2:688712/100‑1 (MQ=255)
                                                                               caCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGa                  <  1:923328/100‑1 (MQ=255)
                                                                                aCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGa                  <  1:2895633/99‑1 (MQ=255)
                                                                                  ggCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATaa               >  2:599785/1‑100 (MQ=255)
                                                                                   gCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAg              <  1:1947687/100‑1 (MQ=255)
                                                                                    cAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGc             >  2:164157/1‑100 (MQ=255)
                                                                                     aGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGcc            <  2:1914296/100‑1 (MQ=255)
                                                                                     aGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATAAACAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGcc            <  1:884370/100‑1 (MQ=255)
                                                                                       cggcgAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTg          >  2:467548/1‑100 (MQ=255)
                                                                                        ggcgAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTg          >  1:3321329/1‑100 (MQ=255)
                                                                                          cgAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAAt       <  1:1079789/100‑1 (MQ=255)
                                                                                                tAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTatgg  <  1:2308282/100‑1 (MQ=255)
                                                                                                 |                                                                                                  
GAAATTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTATGG  >  NC_000913/1170236‑1170431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: