Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 584,262 (A)7→8 coding (582/750 nt) appY → global transcriptional activator; DLP12 prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913584,2551.A93.4% 493.7 / 24.9 151coding (575/750 nt)appYglobal transcriptional activator; DLP12 prophage
Reads supporting (aligned to +/- strand):  ref base . (5/5);  new base A (66/75);  total (71/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 174 total aligned reads displayed.

GGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAG  >  NC_000913/584157‑584350
                                                                                                   |                                                                                                 
      cttttATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCaaaaaaa                                                                                           <  1:3422314/98‑1 (MQ=255)
       aTTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCaaaaaaaa                                                                                          >  2:1044977/1‑99 (MQ=255)
       aTTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATAGTGAGAGATACTAGGCTGAGTTCTGCaaaaaaca                                                                                           >  1:968104/1‑98 (MQ=255)
        tttATACGAGTGACAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAATTATTGAGCGATACTAGGATGAGGTATGCaaaaaaaac                                                                                         >  1:1068953/1‑98 (MQ=255)
        tttATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCaaaaaaaac                                                                                         >  2:141986/1‑98 (MQ=255)
        tttATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGACGTATGCaaaaaaaag                                                                                         >  2:3422366/1‑98 (MQ=255)
        tttATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAAGAATGCaaaaaaaac                                                                                         <  1:2765787/100‑3 (MQ=255)
         ttATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCaaaaaaaact                                                                                        <  2:155951/100‑4 (MQ=255)
          tataCGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCaaaaaaaactc                                                                                       <  2:367316/100‑5 (MQ=255)
           ataCGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCaaaaaaaactca                                                                                      >  2:3367741/1‑95 (MQ=255)
             aCGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATa                                                                                    >  2:3221145/1‑100 (MQ=255)
              cGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATaa                                                                                   >  1:2534130/1‑100 (MQ=255)
                 gTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACtt                                                                                >  2:663815/1‑100 (MQ=255)
                  tGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACtt                                                                                <  1:1308777/99‑1 (MQ=255)
                  tGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTc                                                                               >  1:2852637/1‑100 (MQ=255)
                  tGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTc                                                                               <  2:145576/100‑1 (MQ=255)
                  tGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTc                                                                               <  2:48477/100‑1 (MQ=255)
                   gAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATaagatcg                                                                              >  2:1655686/1‑95 (MQ=255)
                   gAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTc                                                                               <  1:86008/99‑1 (MQ=255)
                   gAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCa                                                                              >  2:497207/1‑100 (MQ=255)
                   gAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATAATAGGATGAGGTATGCAAAAAAAACTCATAAATTCa                                                                              >  1:797902/1‑100 (MQ=255)
                    aaaGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCaa                                                                             >  1:132092/1‑100 (MQ=255)
                    aaaGTATAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAAGTATTCAAAAAAAACTCATAACTTCa                                                                              <  1:1656978/99‑1 (MQ=255)
                     aaGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCaaa                                                                            <  2:418157/100‑1 (MQ=255)
                      aGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAAc                                                                           <  2:55418/100‑1 (MQ=255)
                      aGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAAc                                                                           >  2:2085977/1‑100 (MQ=255)
                      aGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAAATTCaaa                                                                            >  1:987824/1‑99 (MQ=255)
                       gTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAAct                                                                          >  2:3167740/1‑100 (MQ=255)
                          taataaAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCtt                                                                       <  2:937395/100‑1 (MQ=255)
                          taataaAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCtt                                                                       <  1:784379/100‑1 (MQ=255)
                          taataaAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGATATGCAAAAAAAACTCATAACTTCAAActc                                                                         <  1:3332125/98‑1 (MQ=255)
                            ataaAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAACATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTAt                                                                     <  1:1444346/100‑1 (MQ=255)
                            aaaaaaaaaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTAt                                                                     <  1:1584827/98‑1 (MQ=255)
                              aaaaaaaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTc                                                                   <  2:2211251/100‑1 (MQ=255)
                              aaaaaaaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTc                                                                   >  2:441926/1‑100 (MQ=255)
                               aaaaaaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCt                                                                  >  1:1649404/1‑100 (MQ=255)
                                aaaaaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTa                                                                 <  2:2933038/100‑1 (MQ=255)
                                aaaaaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTa                                                                 <  2:3002776/100‑1 (MQ=255)
                                 aaaaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTAt                                                                >  2:1542689/1‑100 (MQ=255)
                                 aaaaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTAt                                                                >  1:1477732/1‑100 (MQ=255)
                                 aaaaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAAGAAAGAAAAAAAAACTCATAACTTCAAACTCTTATTCTAt                                                                <  1:874968/100‑1 (MQ=255)
                                   aaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCa                                                              >  2:3191105/1‑100 (MQ=255)
                                    aaGATTAAGGGATGAAGGAAAGTCATTTACTAAAATATTGAGAGATACTAGGATGAAGTATGAAAAAAAAACTCATAACTTCAAACTCTTATTCTATCaa                                                             <  1:1686752/100‑1 (MQ=255)
                                      gATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGCGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTTTATCAAt                                                            >  2:1901412/1‑99 (MQ=255)
                                      gATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATg                                                           <  2:367794/100‑1 (MQ=255)
                                      gATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATg                                                           <  2:1431090/100‑1 (MQ=255)
                                        ttAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTc                                                         >  1:3384437/1‑100 (MQ=255)
                                         tAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCg                                                        >  2:709768/1‑100 (MQ=255)
                                          aaGGCATGAAGGTACATCATCTACTGAAATATTGAGAGACACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGt                                                       <  1:12655/100‑1 (MQ=255)
                                           aGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTa                                                      >  1:1036983/1‑100 (MQ=255)
                                            gggATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAg                                                     >  2:2747681/1‑100 (MQ=255)
                                            gggATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAg                                                     >  2:3150532/1‑100 (MQ=255)
                                             ggATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAg                                                     >  1:2375990/1‑99 (MQ=255)
                                             ggATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGc                                                    <  2:2522908/100‑1 (MQ=255)
                                              gATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGcc                                                   <  2:2139456/100‑1 (MQ=255)
                                               atgaatgaACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGccc                                                  <  2:1140373/100‑1 (MQ=255)
                                               aTGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGccc                                                  <  2:2880764/100‑1 (MQ=255)
                                               aTGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGccc                                                  <  2:2032307/100‑1 (MQ=255)
                                                tGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCa                                                 >  2:3126986/1‑100 (MQ=255)
                                                 gAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAg                                                >  2:1703455/1‑100 (MQ=255)
                                                   aGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGaa                                              <  2:2741981/100‑1 (MQ=255)
                                                   aGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAAGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGaa                                              >  1:1903398/1‑100 (MQ=255)
                                                        cGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAGtgtg                                         <  2:880559/100‑1 (MQ=255)
                                                         gTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAGTGTCGTAGCCCAGAAATGTgg                                        >  1:2265948/1‑100 (MQ=255)
                                                          tCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGc                                       <  1:2034548/100‑1 (MQ=255)
                                                          tCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGc                                       <  2:2990348/100‑1 (MQ=255)
                                                          tCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCGTATTCTATCAATGTCGTAGCCCAGAAATGTGGc                                       <  2:1064985/99‑1 (MQ=255)
                                                           cATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCt                                      >  2:2420492/1‑100 (MQ=255)
                                                            aTTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTAGTCTATCAATGTCGTAGCCCAGAAATGTGGCta                                     <  2:1345710/100‑1 (MQ=255)
                                                            aTTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCAGAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCta                                     <  2:1410119/100‑1 (MQ=255)
                                                               tagtGAAATATTGAGAGATACTAGGATGAAAAAAAAAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCt                                      <  1:334992/93‑1 (MQ=255)
                                                               tACTGAAATATTGAGAGATACTAGGATGAGATATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCtat                                    <  1:42763/97‑1 (MQ=255)
                                                                 cTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACa                                >  1:391919/1‑100 (MQ=255)
                                                                 cTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATAAATGTATTAGCCCAGAAATGTGGCTATAACa                                >  2:639768/1‑100 (MQ=255)
                                                                  tGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGt                              <  2:1308032/100‑1 (MQ=255)
                                                                   gAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGt                              >  1:1512760/1‑100 (MQ=255)
                                                                   gAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAAATCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAg                               >  1:3191322/1‑99 (MQ=255)
                                                                     aaTATTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTAc                            <  2:1370674/100‑1 (MQ=255)
                                                                        atTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttc                         >  1:353287/1‑100 (MQ=255)
                                                                        atTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttc                         >  1:9549/1‑100 (MQ=255)
                                                                        atTGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttc                         <  2:746442/100‑1 (MQ=255)
                                                                         ttGAGATATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttca                        <  1:2241743/100‑1 (MQ=255)
                                                                         ttGAGAGATACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttca                        <  2:3077919/100‑1 (MQ=255)
                                                                           gagagaTACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcata                      <  1:3242195/100‑1 (MQ=255)
                                                                            agagaTACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatat                     <  1:1754742/100‑1 (MQ=255)
                                                                              agaTACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatattt                   <  1:2773733/100‑1 (MQ=255)
                                                                               gaTCCTAGGATGAGGTATGAAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatatttc                  <  2:987836/100‑1 (MQ=255)
                                                                               gaTACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatatttc                  <  1:3262319/100‑1 (MQ=255)
                                                                               gaTACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatatttc                  <  2:2265980/100‑1 (MQ=255)
                                                                                 tACTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatatttcat                >  2:2615166/1‑100 (MQ=255)
                                                                                  aCTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatatttcata               >  1:3177758/1‑100 (MQ=255)
                                                                                   cTAGGATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatatttcatat              >  2:3325182/1‑100 (MQ=255)
                                                                                      ggATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATAtgtg           <  2:1074867/100‑1 (MQ=255)
                                                                                      ggATGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATAtgtg           <  2:1879723/100‑1 (MQ=255)
                                                                                         tGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCAt        >  2:675782/1‑100 (MQ=255)
                                                                                         tGAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCAt        <  1:730259/100‑1 (MQ=255)
                                                                                          gAGGTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCAtt       >  2:2431140/1‑100 (MQ=255)
                                                                                            ggTATGCAAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTa     >  2:1338312/1‑100 (MQ=255)
                                                                                            ggTATGCAAAAAAGACTCATAACTTCAAACTCTTATTCTATCGATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTa     >  2:2354473/1‑100 (MQ=255)
                                                                                               aTGCAAAAAAAGCTCATAACTTCAAACACTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAg  <  2:890710/100‑1 (MQ=255)
                                                                                                   |                                                                                                 
GGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAG  >  NC_000913/584157‑584350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: