Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,674,9550AT27.0% 243.4 / 115.0 152intergenic (+8/+17)tqsA/pntBpheromone AI‑2 transporter/pyridine nucleotide transhydrogenase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (59/52);  new base T (16/25);  total (75/77)
Fisher's exact test for biased strand distribution p-value = 1.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.55e-01

Only 100 of 164 total aligned reads displayed.

TGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGT  >  NC_000913/1674856‑1675054
                                                                                                   |                                                                                                   
tGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTca                                                                                                     >  2:1228682/1‑100 (MQ=255)
  attttGACAATAATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagc                                                                                                   >  1:2558338/4‑100 (MQ=255)
   cGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagca                                                                                                  >  1:2518638/1‑100 (MQ=255)
   cGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagca                                                                                                  <  1:3051400/100‑1 (MQ=255)
       gACAATTATTGTCAGAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAgg                                                                                              <  1:807099/100‑1 (MQ=255)
        aCAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGc                                                                                             <  1:2187757/100‑1 (MQ=255)
        aCAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGc                                                                                             <  2:689115/100‑1 (MQ=255)
         cAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGcc                                                                                            >  2:1313504/1‑100 (MQ=255)
           attattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGTGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGt                                                                                          <  1:1862646/100‑1 (MQ=255)
           attattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGt                                                                                          >  2:1112892/1‑100 (MQ=255)
            ttattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTc                                                                                         <  1:1172932/100‑1 (MQ=255)
             tattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTc                                                                                         <  1:1504309/99‑1 (MQ=255)
              attGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCac                                                                                       <  2:177721/100‑2 (MQ=255)
              attGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCac                                                                                       <  2:1935358/100‑2 (MQ=255)
              attGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCac                                                                                       <  2:2265467/100‑2 (MQ=255)
              attGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCac                                                                                       <  2:25657/100‑2 (MQ=255)
              attGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTATGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCa                                                                                        <  1:386380/99‑1 (MQ=255)
               ttGTCAAAATTGCGCTTGAACAAACAGCGGGGGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCact                                                                                      <  2:1069843/100‑3 (MQ=39)
                tGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCac                                                                                       <  2:128733/98‑2 (MQ=255)
                tGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAggg                                                                                     <  2:1639052/100‑1 (MQ=255)
                tGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAggg                                                                                     >  2:2425123/1‑100 (MQ=255)
                 gTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGcagatcggaa                                                                                    >  1:3043170/1‑91 (MQ=255)
                 gTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGt                                                                                    >  1:1849372/1‑100 (MQ=255)
                  tCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCactctt                                                                                   <  2:1272609/100‑6 (MQ=38)
                   cAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTa                                                                                  >  1:1113233/1‑100 (MQ=255)
                    aaaaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTAc                                                                                 <  2:745642/100‑1 (MQ=255)
                     aaaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCactctttat                                                                                <  2:2777728/100‑9 (MQ=38)
                      aaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCactctttatt                                                                               <  2:1689824/100‑10 (MQ=37)
                      aaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACag                                                                               <  1:142169/100‑1 (MQ=255)
                      aaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACag                                                                               <  2:1503122/100‑1 (MQ=255)
                         tGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGc                                                                            <  1:352623/100‑1 (MQ=255)
                           cgcTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCtt                                                                          >  1:661699/1‑100 (MQ=255)
                             cTTGAACAAACAGCGGGGGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGTCGGCCTCATCAGAGGCCGTCAGGGTTACAGAGCtt                                                                          <  1:1039743/98‑1 (MQ=255)
                             cTTGAACAAACAGCGGGAGGTCAGAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTc                                                                        >  1:120929/1‑100 (MQ=255)
                             cTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTc                                                                        >  2:513544/1‑100 (MQ=255)
                             cTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTc                                                                        >  2:1711311/1‑100 (MQ=255)
                                gAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCGTTCAgg                                                                     >  1:3171471/1‑100 (MQ=255)
                                 aacaaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGa                                                                    >  2:2407910/1‑100 (MQ=255)
                                  acaaacaGCGTGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGAt                                                                   <  1:1536383/100‑1 (MQ=255)
                                   caaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGAtt                                                                  >  1:3006260/1‑100 (MQ=255)
                                    aaacaGCGGGAGGTCAGAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTg                                                                 <  2:120931/100‑1 (MQ=255)
                                     aacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGc                                                                <  2:2078017/100‑1 (MQ=255)
                                     aacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGc                                                                >  1:336709/1‑100 (MQ=255)
                                     aacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGc                                                                <  1:528414/100‑1 (MQ=255)
                                      acaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCa                                                               <  1:1371866/100‑1 (MQ=255)
                                      acaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCa                                                               <  1:1729394/100‑1 (MQ=255)
                                       caGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCAt                                                              <  1:513538/100‑1 (MQ=255)
                                         ncGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATcc                                                            >  2:1537725/2‑100 (MQ=255)
                                           gggAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCAc                                                          >  1:3160740/1‑100 (MQ=255)
                                            ngAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACg                                                         >  1:96983/2‑100 (MQ=255)
                                            ggAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACg                                                         >  1:2215112/1‑100 (MQ=255)
                                              aGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCt                                                       >  1:256259/1‑100 (MQ=255)
                                              aGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCt                                                       <  1:153707/100‑1 (MQ=255)
                                                gTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTgg                                                     <  1:1435811/100‑1 (MQ=255)
                                                 tCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGc                                                    >  2:1975984/1‑100 (MQ=255)
                                                  cAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCt                                                   >  1:1647930/1‑100 (MQ=255)
                                                   aaaGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCtt                                                  >  2:435286/1‑100 (MQ=255)
                                                     aGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTg                                                >  2:979215/1‑100 (MQ=255)
                                                      gCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTgg                                               <  1:342099/100‑1 (MQ=255)
                                                       cATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCCGGCTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGc                                              >  1:2437198/1‑100 (MQ=255)
                                                       cATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGc                                              >  2:346943/1‑100 (MQ=255)
                                                        aTCGCCGTTCTGTTAAGCGATCTCAGTAAAGGGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGcn                                             <  1:709049/100‑2 (MQ=255)
                                                          cGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTc                                           >  1:876283/1‑100 (MQ=255)
                                                           gCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCa                                          <  2:1437014/100‑1 (MQ=255)
                                                           gCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCa                                          <  2:2702269/100‑1 (MQ=255)
                                                             cGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCAcc                                        <  1:2618715/100‑1 (MQ=255)
                                                             cGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCAcc                                        <  2:3000367/100‑1 (MQ=255)
                                                                  tGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACa                                   >  1:3126196/1‑100 (MQ=255)
                                                                  tGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACa                                   <  1:2407877/100‑1 (MQ=255)
                                                                  tGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACa                                   <  1:832621/100‑1 (MQ=255)
                                                                  tGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACa                                   >  2:3011952/1‑100 (MQ=255)
                                                                    ttAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGc                                 <  2:1647953/100‑1 (MQ=255)
                                                                          gATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTgg                           <  2:2440048/100‑1 (MQ=255)
                                                                           aTCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTggg                          <  1:957087/100‑1 (MQ=255)
                                                                           aTCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTggg                          <  1:1991894/100‑1 (MQ=255)
                                                                              tcAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGgtgt                       <  1:979203/100‑1 (MQ=255)
                                                                               cAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGtt                      >  1:1206090/1‑100 (MQ=255)
                                                                                aaTAAAGACTGACGGCCTCAGCAGAGGCCGTCAGTTTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCTTCACCAAACAGCATGTggg                          <  1:793216/95‑1 (MQ=255)
                                                                                  tAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGGGTTTTc                   <  1:1699097/100‑1 (MQ=255)
                                                                                  gtaaccctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTc                   >  1:2699063/8‑100 (MQ=38)
                                                                                      gagTGACGGCCTCAGCAGAGGCCGTCGGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTg               >  2:3336378/1‑100 (MQ=255)
                                                                                      gagTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTg               <  2:3185103/100‑1 (MQ=255)
                                                                                      ccctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTg               >  2:1574816/4‑100 (MQ=39)
                                                                                      ccctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTg               >  2:3458546/4‑100 (MQ=255)
                                                                                      ccctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTAGCGTCAACAAACAGCATGTGGGTGTTTTCCTTg               >  1:786024/4‑100 (MQ=38)
                                                                                        gTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa             <  1:2372547/100‑1 (MQ=255)
                                                                                        ctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa             >  1:464370/2‑100 (MQ=255)
                                                                                        ctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa             >  2:2887487/2‑100 (MQ=255)
                                                                                         tGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaag            <  1:3324797/100‑1 (MQ=255)
                                                                                         tGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaag            >  1:1292571/1‑100 (MQ=255)
                                                                                         tGACGGCCTCAGCAGAGGCTGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTAGCTTTGGCGTCACCAAACAGCATGTGGTTGTTTTCCTGgaag            <  1:620680/100‑1 (MQ=255)
                                                                                          gACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTccagatcgg           >  1:1338521/1‑93 (MQ=255)
                                                                                          gACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaaga           >  2:2305375/1‑100 (MQ=255)
                                                                                          gACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaaga           >  2:3324848/1‑100 (MQ=255)
                                                                                          gACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaaga           >  2:1323893/1‑100 (MQ=255)
                                                                                            cGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAAc         >  2:54623/1‑100 (MQ=255)
                                                                                               ccTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGc      >  1:876269/1‑100 (MQ=255)
                                                                                                 tCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCgg    <  2:10620/100‑1 (MQ=255)
                                                                                                  cagcagAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCggg   <  2:1209750/100‑1 (MQ=255)
                                                                                                   agcagAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGt  <  2:662701/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
TGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGT  >  NC_000913/1674856‑1675054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: