Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9840TG43.9% 262.9 / inf 278intergenic (‑194/+114)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base T (80/76);  new base G (114/8);  total (194/84)
Fisher's exact test for biased strand distribution p-value = 2.24e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.17e-02

Only 100 of 282 total aligned reads displayed.

TTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCT  >  NC_000913/3705885‑3706083
                                                                                                   |                                                                                                             
     gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                                                          <  2:2275410/100‑1 (MQ=255)
        ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGc                                                                                                       <  2:2753336/100‑1 (MQ=37)
           tGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGCATa                                                                                                    <  1:1215384/100‑1 (MQ=37)
            ggCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGCATaa                                                                                                   <  1:1010223/100‑2 (MQ=25)
             gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                                                 >  2:460815/1‑100 (MQ=255)
              cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaa                                                                                                <  2:558666/100‑1 (MQ=255)
                cTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaaa                                                                                              >  2:2927379/1‑100 (MQ=255)
                  gCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATc                                                                                            >  2:2432225/1‑100 (MQ=255)
                  gCACGCAGAGGCACAGCGCCACCGGGTATACAGATAGTTGACAAAGTGCGCTTTGGTTAAGCCAGATGAGGGGTAAACTCCTTATCCGGCCTACAAAATc                                                                                            >  1:2186233/1‑100 (MQ=21)
                    aCGGAGAGGCACAGCGCCACCGAGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATc                                                                                            <  1:2413519/98‑1 (MQ=255)
                    aCGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGt                                                                                          >  1:1478884/1‑100 (MQ=255)
                     cGCAGATGAACACCGCCACCGGGTTTACATATTTTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGTCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                                         <  2:3290495/100‑1 (MQ=17)
                      gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                        <  1:708527/100‑1 (MQ=255)
                       cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                                       >  1:2811676/1‑100 (MQ=255)
                         gagGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCaaa                                                                                     <  1:1108216/100‑1 (MQ=255)
                          agGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAt                                                                                    <  2:2584305/100‑1 (MQ=255)
                               caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                                               <  2:3049609/100‑1 (MQ=255)
                                aGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATa                                                                              <  1:2702218/100‑1 (MQ=255)
                                    ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                                          >  2:417166/1‑100 (MQ=255)
                                    ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                                          <  2:1550325/100‑1 (MQ=255)
                                      aCCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGc                                                                        <  2:1082357/100‑1 (MQ=255)
                                            tataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAt‑‑t                                                              <  1:3343268/100‑1 (MQ=255)
                                              taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAt‑‑t‑‑‑tt                                                         <  2:212359/100‑1 (MQ=255)
                                                cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑g                                                     >  1:978383/1‑100 (MQ=255)
                                                 aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGGGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑Gt                                                    >  1:1851281/1‑100 (MQ=255)
                                                   ggcataaaCAAAGCGCACTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAg                                                  >  2:2701999/8‑100 (MQ=18)
                                                       ttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCt                                              >  2:2837289/1‑100 (MQ=255)
                                                        tgACAAAGTGCTGTTTGGTTCTGCCGGAGGCGGCGTGAACGCCTTATCCGGCCTACAAAATCGTGCAGATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTg                                             <  1:1577931/100‑1 (MQ=17)
                                                        tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTg                                             >  1:1367345/1‑100 (MQ=255)
                                                              aGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGc                                       <  2:3334909/100‑1 (MQ=255)
                                                               gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGTCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCg                                      <  2:1683037/100‑1 (MQ=255)
                                                                    gattttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCAT‑‑C‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAgcg                                 >  2:2776649/11‑100 (MQ=18)
                                                                     tttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgcgc                                <  1:511862/100‑1 (MQ=255)
                                                                       tGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCAt                              >  2:797891/1‑100 (MQ=255)
                                                                        ttgtaggCCGGATAAGGCGTTTACGCCGCATCTGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAA‑‑T‑‑‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATc                             >  2:3229828/7‑100 (MQ=18)
                                                                        gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATc                             <  1:1944220/100‑1 (MQ=255)
                                                                          ttATGCCGGATGCGGCGTTAACGCCTTATCCTGCCTACAAAATCGTGCAAATTCAATACATGGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGAGAAGCGGAGCGCATCAg                           >  1:2027745/1‑100 (MQ=255)
                                                                          ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAg                           >  1:2256146/1‑100 (MQ=255)
                                                                          gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                          >  2:2219361/5‑100 (MQ=18)
                                                                          gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAT‑‑TCAACTT‑‑GTAGGCCTGATAAGCGCAGCGCATCa                            >  2:2191371/5‑100 (MQ=21)
                                                                          gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATCT‑‑C‑‑‑CGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                           >  2:3063648/5‑100 (MQ=18)
                                                                           tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAgg                          >  2:1173356/1‑100 (MQ=255)
                                                                            aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGT‑‑T‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                         >  2:2596096/3‑100 (MQ=17)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACGAAATCGTGCTAATTCAATATATTGTGGGCT‑‑C‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                         >  2:3200515/3‑100 (MQ=18)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAG‑‑C‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCa                            >  2:1134503/3‑97 (MQ=17)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                         >  2:1472879/3‑100 (MQ=14)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAAT‑‑C‑‑‑TCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGc                         >  1:2502633/3‑100 (MQ=18)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAG‑‑T‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                        >  2:1230055/3‑100 (MQ=21)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAAACTGTGCAAATTCAATATATTGCAGGAA‑‑A‑‑‑CAC‑‑GTAGGCCTGATAAGCGAAGCGCATCAGGc                         >  1:381332/3‑100 (MQ=18)
                                                                            aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc                         <  2:265218/100‑1 (MQ=255)
                                                                             ggtcggataaggcgTTCACGCCGCATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAAC‑‑G‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                        >  2:2732408/11‑100 (MQ=21)
                                                                             ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAt                       >  2:394775/2‑100 (MQ=18)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGAC‑‑C‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                        >  2:2461310/2‑100 (MQ=18)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGAC‑‑C‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                        >  2:1039480/2‑100 (MQ=18)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAG‑‑C‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCa                            >  2:2083269/2‑96 (MQ=17)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGAT‑‑A‑‑‑CGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                        >  1:843489/2‑100 (MQ=18)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGACTACAAAACTTTGCAAATTCAATATATTGCAAT‑‑C‑‑‑TCCGTGTAGGCCTGATAAGCGTAGAGCATCAGGCa                        >  1:2289709/2‑100 (MQ=18)
                                                                              gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtt                      >  2:1851877/1‑100 (MQ=18)
                                                                              gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGAC‑‑C‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag                       >  2:2512027/1‑99 (MQ=18)
                                                                              gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCAT‑‑C‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGcga                       >  2:1795037/1‑98 (MQ=21)
                                                                              gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAG‑‑T‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtt                      >  1:102586/1‑100 (MQ=21)
                                                                               ccggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGT‑‑T‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                      >  2:681183/9‑98 (MQ=17)
                                                                               ccggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGT‑‑T‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                      >  2:1373414/9‑98 (MQ=17)
                                                                               ccggataaggcgTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTT‑‑A‑‑‑TTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaat                      >  2:628674/9‑98 (MQ=21)
                                                                               ccggataaggcgTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCAT‑‑C‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaat                      >  2:1743473/9‑98 (MQ=21)
                                                                               ccggataaggcgTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCacg                      >  2:1298077/9‑98 (MQ=17)
                                                                               ccggataaggcgTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGTAGCGCAGCAgg                          >  1:3045634/9‑96 (MQ=14)
                                                                               ccggataaggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGAC‑‑T‑‑‑AT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCagc                      >  1:2781434/9‑98 (MQ=18)
                                                                               ccGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtt                      >  2:958944/1‑100 (MQ=18)
                                                                                cggataaggcgTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGATCGAT‑‑C‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagtt                     >  2:747371/8‑97 (MQ=21)
                                                                                cggataaggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGAC‑‑T‑‑‑AT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCagct                     >  2:3039669/8‑97 (MQ=18)
                                                                                 ggataaggcgTTTACGCCTTATCCGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAA‑‑T‑‑‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAattt                    >  1:642239/7‑100 (MQ=18)
                                                                                 ggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGT‑‑T‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                    >  1:1664766/7‑100 (MQ=17)
                                                                                 ggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGT‑‑T‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                    >  2:3447080/7‑100 (MQ=17)
                                                                                 ggataaggcgTTTACGCCGCATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAA‑‑‑CTT‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaatt                     >  2:1937670/7‑97 (MQ=21)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCGTACGAAATCGTGCTAATTCAATATATTGTGGGCT‑‑C‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                    >  2:923982/7‑100 (MQ=18)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGAC‑‑T‑‑‑AT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCagctt                    >  1:2680959/7‑96 (MQ=18)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGAT‑‑T‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAattt                    >  2:1387556/7‑100 (MQ=17)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAG‑‑T‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtttat                   >  2:1994532/7‑98 (MQ=21)
                                                                                 ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtttt                    >  2:2951293/1‑100 (MQ=255)
                                                                                 ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtttt                    >  2:197564/1‑100 (MQ=255)
                                                                                  gataaggcgTTCACGCCGCATCCGGCCTACAAAAGCACGCAAACTCAATATATTGCAGAGAT‑‑C‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAattta                   >  1:2575390/6‑99 (MQ=17)
                                                                                   ataaggcgTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGATCGAT‑‑C‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAGTtttg                  >  1:374889/5‑100 (MQ=21)
                                                                                     gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc                <  2:2561252/100‑1 (MQ=255)
                                                                                     gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc                <  1:2951246/100‑1 (MQ=255)
                                                                                     aaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGT‑‑T‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAGTtttgcg                >  1:2012771/3‑99 (MQ=17)
                                                                                      cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCTGGCATTtttgcgt               <  2:2359807/100‑3 (MQ=255)
                                                                                       ggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctt              >  2:2058617/1‑100 (MQ=255)
                                                                                       ggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGAGAATCAGGCATTtttgc                 >  1:3231777/1‑97 (MQ=255)
                                                                                        gcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgccttt             <  2:2314379/100‑1 (MQ=255)
                                                                                        gcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATGCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgccttt             <  1:3290504/100‑1 (MQ=255)
                                                                                         cgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttg            <  2:36698/100‑1 (MQ=255)
                                                                                          gTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc           <  1:2225914/100‑1 (MQ=255)
                                                                                          gTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc           >  1:1338954/1‑100 (MQ=255)
                                                                                             aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCAt        >  1:1701729/1‑100 (MQ=255)
                                                                                             aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCAt        >  1:211583/1‑100 (MQ=255)
                                                                                              aCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATc       <  2:3412789/100‑1 (MQ=255)
                                                                                                gCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAg     <  2:3009335/100‑1 (MQ=255)
                                                                                                  cTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTc   >  2:2888958/1‑100 (MQ=255)
                                                                                                   ttATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCt  <  2:876350/100‑1 (MQ=255)
                                                                                                   |                                                                                                             
TTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCT  >  NC_000913/3705885‑3706083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: