Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,706,0170CT35.1% 332.2 / 229.4 269intergenic (‑227/+81)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base C (82/77);  new base T (82/4);  total (188/81)
Fisher's exact test for biased strand distribution p-value = 6.03e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.98e-01

Only 100 of 270 total aligned reads displayed.

CCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTT  >  NC_000913/3705921‑3706116
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ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                                                                        <  2:1550325/100‑1 (MQ=255)
  aCCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGc                                                                                                      <  2:780017/100‑1 (MQ=255)
        tataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAt‑‑t                                                                                            >  2:1016478/1‑100 (MQ=255)
          taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAt‑‑ttt                                                                                          <  2:212359/100‑1 (MQ=255)
            cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTc                                                                                         >  1:1693706/1‑99 (MQ=255)
            cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑g                                                                                      <  2:1099787/100‑1 (MQ=255)
             aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGGGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑Gt                                                                                     >  1:1851281/1‑100 (MQ=255)
              gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTa                                                                                    >  1:1985488/1‑100 (MQ=255)
               ggcataaaCAAAGCGCACTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAg                                                                                   >  2:2701999/8‑100 (MQ=18)
               aTTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAg                                                                                   >  2:1950292/1‑100 (MQ=255)
                 tgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGc                                                                                 <  2:1707828/100‑1 (MQ=255)
                     gACAAAGTGCGCTTTGGTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTg                                                                              >  2:511868/1‑99 (MQ=255)
                       cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATa                                                                           >  1:3334857/1‑100 (MQ=255)
                       cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATa                                                                           >  2:1754236/1‑100 (MQ=255)
                           gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGTCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCg                                                                       <  2:1683037/100‑1 (MQ=255)
                              cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTTAATACATTGCAGAAT‑‑TTTC‑‑GTAGGTCTGATAAGCGGAg                                                                    <  1:2149784/100‑1 (MQ=255)
                                 tttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAgcgc                                                                 <  1:511862/100‑1 (MQ=255)
                                     tgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCAT‑‑CCTC‑‑GTAGGCCTGATAAGCGTAGCGCATCa                                                             >  2:2587878/6‑100 (MQ=21)
                                      ttATGCCGGAGGCGGCGTTAACGCCTTGTCCGGCCTACAAAATCGTGCAAATTGAATACATTGCAGAAT‑‑GTTC‑‑GTAGGCCTGAGAAGCGGAGCGCATCAg                                                            >  1:2027701/1‑100 (MQ=18)
                                      gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                                                           >  2:2219361/5‑100 (MQ=18)
                                      gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCAT‑‑CCTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                                                            >  2:2786412/5‑100 (MQ=21)
                                      gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAG‑‑TTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                                                           >  1:2357101/5‑100 (MQ=21)
                                       tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAgg                                                           >  2:1173356/1‑100 (MQ=255)
                                        aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGC‑‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                                                          >  2:3042451/3‑100 (MQ=17)
                                        aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAG‑‑CCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCa                                                             >  2:1134503/3‑97 (MQ=17)
                                        aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAG‑‑TTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                         >  2:1230055/3‑100 (MQ=21)
                                        aggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAAACTGTGCAAATTCAATATATTGCAGGAA‑‑ACAC‑‑GTAGGCCTGATAAGCGAAGCGCATCAGGc                                                          >  1:381332/3‑100 (MQ=18)
                                        aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc                                                          >  2:409009/1‑100 (MQ=255)
                                        aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc                                                          <  2:265218/100‑1 (MQ=255)
                                         ggtcggataaggcgTTCACGCCGCATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAAC‑‑GAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                         >  2:2732408/11‑100 (MQ=21)
                                         ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAACAGTGCAAATTCAATATATTGCAATCT‑‑TCTG‑‑GTAGGCCTGATAAGCATCGCGCATCAGGCa                                                         >  2:3306163/2‑100 (MQ=18)
                                         ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAG‑‑CCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCa                                                             >  2:2083269/2‑96 (MQ=17)
                                         ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGAT‑‑ACGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                         >  1:843489/2‑100 (MQ=18)
                                         ggCCGGATAAGGCGTTCACGCCGCATCCGGACTACAAAACTTTGCAAATTCAATATATTGCAAT‑‑CTCCGTGTAGGCCTGATAAGCGTAGAGCATCAGGCa                                                         >  1:2289709/2‑100 (MQ=18)
                                         ggCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa                                                         >  1:1578152/2‑100 (MQ=17)
                                          gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt                                                        >  1:2828268/1‑100 (MQ=255)
                                          gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtt                                                       >  2:1851877/1‑100 (MQ=18)
                                          gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGAC‑‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag                                                        >  2:2512027/1‑99 (MQ=18)
                                          gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGATCGAT‑‑CCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag                                                        >  2:634121/1‑99 (MQ=21)
                                          gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGAT‑‑ACGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa                                                        >  2:2739321/1‑99 (MQ=17)
                                          gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAG‑‑TTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtt                                                       >  1:102586/1‑100 (MQ=21)
                                           ctggataaggcgTTCACGCCGCATCCAGCCTACAAAATTGTGCAAAATCAATGGATTGCACAGC‑‑TAGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaga                                                       >  2:59344/9‑98 (MQ=25)
                                           ccggataaggcgTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAttt                                                      >  2:3093070/9‑100 (MQ=18)
                                           ccggataaggcgTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGATCGAT‑‑CCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                                                       >  1:1359262/9‑98 (MQ=18)
                                           ccggataaggcgTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGAT‑‑TTTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCacg                                                       >  2:1298077/9‑98 (MQ=17)
                                           ccggataaggcgTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGAT‑‑TTTC‑‑GTAGGCCTGATAAGCGTAGCGCAGCAgg                                                           >  1:3045634/9‑96 (MQ=14)
                                           ccggataaggcgTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAG‑‑CTGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                                                       >  2:2280278/9‑98 (MQ=18)
                                            ctgataaggcgTTTACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGCCACT‑‑CACCCAGTAGGCCTGATAAGCGCAGCGCATCAGGCaat                                                       >  2:2460863/8‑98 (MQ=25)
                                            cggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTACAGAGT‑‑TCAC‑‑GTAGGACTGATAAGCGTAGCGCATCAGGCagtt                                                      >  1:2069487/8‑97 (MQ=17)
                                            cggataaggcgTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTT‑‑ATTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaatc                                                      >  2:2945656/8‑97 (MQ=25)
                                            cggataaggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGAC‑‑TAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCagct                                                      >  2:3039669/8‑97 (MQ=18)
                                            cggataaggcgTTCACGCCGCATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAAC‑‑GAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaact                                                      >  2:417322/8‑97 (MQ=25)
                                             ggataaggcgTTTACGCCTTATCCGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAA‑‑TCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAattt                                                     >  1:642239/7‑100 (MQ=18)
                                             ggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCGAATTCAATATATTGCAGAGC‑‑TCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                                                     >  1:1900459/7‑100 (MQ=17)
                                             ggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGT‑‑TCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                                                     >  2:3447080/7‑100 (MQ=17)
                                             ggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGT‑‑TCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                                                     >  1:1664766/7‑100 (MQ=17)
                                             ggataaggcgTTTACGCCGCATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTT‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaatt                                                      >  2:821523/7‑97 (MQ=21)
                                             ggataaggcgTTCACGCCGCATCCTGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGA‑‑TGGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtttt                                                     >  2:743749/7‑100 (MQ=14)
                                             ggataaggcgTTCACGCCGCATCCGGCGTACGAAATCGTGCTAATTCAATATATTGTGGGCT‑‑CCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                                                     >  2:923982/7‑100 (MQ=18)
                                             ggataaggcgTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGAT‑‑TTTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCacgct                                                     >  2:2864781/7‑96 (MQ=17)
                                             ggataaggcgTTCACGCCGCATCCGGCCTACAAAAGCACGCAAACTCAATATATTGCAGAGA‑‑TCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAattt                                                     >  1:324604/7‑100 (MQ=17)
                                             ggataaggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGAC‑‑TAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCagctt                                                     >  1:2680959/7‑96 (MQ=18)
                                             ggataaggcgTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGAT‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAattt                                                     >  2:1387556/7‑100 (MQ=17)
                                             ggataaggcgTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAG‑‑TTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtttat                                                    >  2:1994532/7‑98 (MQ=21)
                                             ggataaggcgTTCACGCCGCATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAAC‑‑GAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaacta                                                     >  2:2407115/7‑96 (MQ=25)
                                              gataaggcgTTTACGCCGCATCCGGCCTACAAAACAGTGCAAATTCAATATATTGCAATCT‑‑TCTG‑‑GTAGGCCTGATAAGCATCGCGCATCAGGCaagctt                                                    >  2:1538955/6‑95 (MQ=21)
                                              gataaggcgTTCACGCCGCATCCGGCCTACAAAAGCACGCAAACTCAATATATTGCAGAGAT‑‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAattta                                                    >  1:2575390/6‑99 (MQ=17)
                                              gataaggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGA‑‑CTAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCagcttt                                                    >  2:3387718/6‑95 (MQ=21)
                                                 gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc                                                 <  1:2951246/100‑1 (MQ=255)
                                                 gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc                                                 <  2:2561252/100‑1 (MQ=255)
                                                  cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCTGGCATTtttgcgt                                                <  2:2359807/100‑3 (MQ=255)
                                                   ggcgTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCAT‑‑CCTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaattctgtgtt                                               <  1:2226081/100‑11 (MQ=25)
                                                   ggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGAGAATCAGGCATTtttgc                                                  >  1:3231777/1‑97 (MQ=255)
                                                    gcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgccttt                                              <  2:2314379/100‑1 (MQ=255)
                                                     cgTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGA‑‑TTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAATtttgcgtttg                                             >  1:131119/1‑100 (MQ=17)
                                                     cgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttg                                             <  2:36698/100‑1 (MQ=255)
                                                      gTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc                                            >  2:950791/1‑100 (MQ=255)
                                                            gCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAg                                      <  2:3009335/100‑1 (MQ=255)
                                                              cTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTc                                    >  2:2888958/1‑100 (MQ=255)
                                                               ttATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TATC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGt                                     >  1:2197750/1‑98 (MQ=255)
                                                                 aTCCGGCCTACAAAATCGTGTAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTg                                 <  2:1961075/100‑1 (MQ=255)
                                                                 aTCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTg                                 <  2:3209479/100‑1 (MQ=255)
                                                                 aTCCAGCCTACAAAATCGTGCAAATTTAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGAATCAGGCCTTTTTGCATTTGCCATTAGTCTTg                                 >  1:912106/1‑100 (MQ=21)
                                                                  tCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGt                                <  1:1725289/100‑1 (MQ=255)
                                                                   ccGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTTTTGTa                               >  1:1289863/1‑100 (MQ=255)
                                                                    cGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTAt                              >  1:1458871/1‑100 (MQ=255)
                                                                       ccTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGc                           <  1:2533937/100‑1 (MQ=255)
                                                                         tACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCtt                         <  1:3357653/100‑1 (MQ=255)
                                                                             aaaaTCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCtttt                       <  2:1223838/99‑1 (MQ=255)
                                                                                    tGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTc               >  2:3233226/1‑100 (MQ=255)
                                                                                    tGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTc               >  2:3370788/1‑100 (MQ=255)
                                                                                      cAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGa             >  1:3430093/1‑100 (MQ=255)
                                                                                        aaTTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATa           <  2:2151185/100‑1 (MQ=255)
                                                                                        aaTTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATa           <  1:2452864/100‑1 (MQ=255)
                                                                                          ttCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATaga         >  1:2779279/1‑100 (MQ=255)
                                                                                          ttCAATACATTGCAGAAT‑‑TTTC‑‑CTAGGCCTGATAAGCGGAGCGCATAATGCATATTTGCCTTTGCCATCAGGCTTGTATGGCTTTTAATTATTCGATa           >  1:706362/1‑98 (MQ=37)
                                                                                            cAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAgaga       <  1:1059153/100‑1 (MQ=255)
                                                                                              aTACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACg     <  1:495953/100‑1 (MQ=255)
                                                                                                aCATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGtt   >  1:1541030/1‑100 (MQ=255)
                                                                                                 cATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGttt  <  1:1200172/100‑1 (MQ=255)
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CCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTT  >  NC_000913/3705921‑3706116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: