Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,706,0290TC26.5% 414.0 / 171.5 271intergenic (‑239/+69)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base T (100/80);  new base C (63/2);  total (189/82)
Fisher's exact test for biased strand distribution p-value = 2.13e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.15e-01

Only 100 of 278 total aligned reads displayed.

TACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGT  >  NC_000913/3705931‑3706128
                                                                                                         |                                                                                                     
taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAt‑‑t‑‑‑tt                                                                                                      <  2:212359/100‑1 (MQ=255)
  cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTc                                                                                                     >  1:1693706/1‑99 (MQ=255)
  cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑g                                                                                                  <  2:1099787/100‑1 (MQ=255)
  cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑g                                                                                                  >  1:978383/1‑100 (MQ=255)
   aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGGGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑Gt                                                                                                 >  1:1851281/1‑100 (MQ=255)
    gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTa                                                                                                >  1:1985488/1‑100 (MQ=255)
     ggcataaaCAAAGCGCACTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAg                                                                                               >  2:2701999/8‑100 (MQ=18)
     aTTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAg                                                                                               >  2:1950292/1‑100 (MQ=255)
       tgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGc                                                                                             <  2:1707828/100‑1 (MQ=255)
          tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTg                                                                                          <  1:2698701/100‑1 (MQ=255)
             cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATa                                                                                       >  1:3334857/1‑100 (MQ=255)
                aGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGc                                                                                    <  2:3334909/100‑1 (MQ=255)
                 gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGTCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCg                                                                                   <  2:1683037/100‑1 (MQ=255)
                 gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCg                                                                                   <  2:2119197/100‑1 (MQ=255)
                  tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCgg                                                                                  <  2:3041055/100‑1 (MQ=255)
                    cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAg                                                                                <  1:3380067/100‑1 (MQ=255)
                      cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgcg                                                                              <  2:200376/100‑1 (MQ=255)
                       tttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgcgc                                                                             <  1:511862/100‑1 (MQ=255)
                        ttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACTAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCa                                                                            <  1:2432192/100‑1 (MQ=255)
                         tttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAA‑‑C‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCAt                                                                           >  2:2917761/8‑100 (MQ=18)
                          ttgtaggCCGGATAAGGCGTTTACGCCGCATCTGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAA‑‑T‑‑‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATc                                                                          >  2:3229828/7‑100 (MQ=18)
                           tgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGAT‑‑A‑‑‑CGT‑‑GTAGGCCTGATAAGCGTAGCGCATCa                                                                         >  1:863251/6‑100 (MQ=18)
                            ttATGCCGGAGGCGGCGTTAACGCCTTGTCCGGCCTACAAAATCGTGCAAATTGAATACATTGCAGAAT‑‑G‑‑‑TTC‑‑GTAGGCCTGAGAAGCGGAGCGCATCAg                                                                        >  1:2027701/1‑100 (MQ=18)
                            gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAT‑‑TCAACTT‑‑GTAGGCCTGATAAGCGCAGCGCATCa                                                                         >  2:2191371/5‑100 (MQ=21)
                            gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCAT‑‑C‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                                                                        >  2:2786412/5‑100 (MQ=21)
                            gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATCT‑‑C‑‑‑CGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                                                                        >  2:3063648/5‑100 (MQ=18)
                             tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAgg                                                                       >  2:1173356/1‑100 (MQ=255)
                              aggCCGGATAAGGCGTTCGCGCCGCATCCGGCCTACAAAAGTTTGCAAATTCAATAAATTGCAGAAT‑‑T‑‑‑CAT‑‑GTAGGCCTGATAAGCGAAGCGCATCAGGc                                                                      >  1:66525/3‑100 (MQ=17)
                              aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCAT‑‑C‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                                                                      >  2:2226113/3‑100 (MQ=21)
                              aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAG‑‑T‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                                     >  2:3443466/3‑100 (MQ=21)
                              aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc                                                                      <  2:265218/100‑1 (MQ=255)
                               ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAt                                                                    >  2:394775/2‑100 (MQ=18)
                               ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGG‑‑C‑‑‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                                     >  2:1145500/2‑100 (MQ=17)
                               ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTT‑‑A‑‑‑TTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCa                                                                     >  2:2722978/2‑100 (MQ=25)
                               ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTT‑‑A‑‑‑TTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCa                                                                     >  1:1352763/2‑100 (MQ=21)
                               ggCCGGATAAGGCGTTCACGCCGCATCCGGACTACAAAACTTTGCAAATTCAATATATTGCAAT‑‑C‑‑‑TCCGTGTAGGCCTGATAAGCGTAGAGCATCAGGCa                                                                     >  1:2289709/2‑100 (MQ=18)
                               ggCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa                                                                     >  1:1578152/2‑100 (MQ=17)
                               ggCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa                                                                     >  2:449092/2‑100 (MQ=18)
                                gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtt                                                                   >  2:1851877/1‑100 (MQ=18)
                                gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAA‑‑C‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag                                                                    >  1:218837/1‑99 (MQ=18)
                                gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGT‑‑T‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag                                                                    >  2:3015468/1‑99 (MQ=17)
                                gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGAC‑‑C‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag                                                                    >  2:2512027/1‑99 (MQ=18)
                                gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAG‑‑C‑‑‑CAC‑‑GTAGGCCTGATAATCGTAGCGAATAAgaaaa                                                                    >  2:1368949/1‑96 (MQ=17)
                                gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGAT‑‑A‑‑‑CGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa                                                                    >  2:2739321/1‑99 (MQ=17)
                                gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAG‑‑T‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtt                                                                   >  1:102586/1‑100 (MQ=21)
                                gCCGGATAAGGCGGTCACGCCGCATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAG‑‑C‑‑‑GGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag                                                                    >  2:2586407/1‑99 (MQ=18)
                                 ccggataaggcgTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAA‑‑C‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                                                                   >  2:238962/9‑98 (MQ=18)
                                 ccggataaggcgTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTT‑‑A‑‑‑TTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaat                                                                   >  2:628674/9‑98 (MQ=21)
                                 ccggataaggcgTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGTAGCGCAGCAgg                                                                       >  1:3045634/9‑96 (MQ=14)
                                 ccggataaggcgTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAG‑‑C‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                                                                   >  2:2280278/9‑98 (MQ=18)
                                 ccggataaggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGAC‑‑T‑‑‑AT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCagc                                                                   >  1:2781434/9‑98 (MQ=18)
                                 ccGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAA‑‑C‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                                                                   >  1:61140/1‑98 (MQ=18)
                                  ctgataaggcgTTTACGCCTTATCAGGCCTACAAGATCGTGCAAATTCAACATATTGCCACT‑‑C‑‑‑ACCCAGTAGGCCTGATAAGCGCAGCGCATCAGGCaat                                                                   >  2:2460863/8‑98 (MQ=25)
                                  cggataaggcgTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGATCGAT‑‑C‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagtt                                                                  >  2:747371/8‑97 (MQ=21)
                                  cggataaggcgTTCACGCCGCATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAAC‑‑G‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaact                                                                  >  2:417322/8‑97 (MQ=25)
                                   ggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCGAATTCAATATATTGCAGAGC‑‑T‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                                                                 >  1:1900459/7‑100 (MQ=17)
                                   ggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGT‑‑T‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                                                                 >  1:1664766/7‑100 (MQ=17)
                                   ggataaggcgTTTACGCCGCATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAA‑‑‑CTT‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaatt                                                                  >  2:821523/7‑97 (MQ=21)
                                   ggataaggcgTTCACGCCGCATCCGGCGTACGAAATCGTGCTAATTCAATATATTGTGGGCT‑‑C‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                                                                 >  2:923982/7‑100 (MQ=18)
                                   ggataaggcgTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGATCGAT‑‑C‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                                                                 >  2:527647/7‑100 (MQ=21)
                                   ggataaggcgTTCACGCCGCATCCGGCCTACAAAAGCACGCAAACTCAATATATTGCAGAGA‑‑T‑‑‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAattt                                                                 >  1:324604/7‑100 (MQ=17)
                                   ggataaggcgTTCACGCCGCATCCGGCCTACAAAACTGTGCAAATTCAATATATTGCAGGAA‑‑A‑‑‑CAC‑‑GTAGGCCTGATAAGCGAAGCACATCAGGCAgttt                                                                 >  2:3182987/7‑100 (MQ=18)
                                   ggataaggcgTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAG‑‑T‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtttat                                                                >  2:2554132/7‑98 (MQ=21)
                                   ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtttt                                                                 >  2:2951293/1‑100 (MQ=255)
                                   ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtttt                                                                 >  2:197564/1‑100 (MQ=255)
                                   ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtgt                                                                  >  2:3478379/1‑97 (MQ=255)
                                    gataaggcgTTTACGCCGCATCCGGCCTACAAAACAGTGCAAATTCAATATATTGCAATCT‑‑T‑‑‑CTG‑‑GTAGGCCTGATAAGCATCGCGCATCAGGCaagctt                                                                >  2:1538955/6‑95 (MQ=21)
                                    gataaggcgTTCACGCCGCATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAA‑‑C‑‑‑GAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaactac                                                                >  1:720422/6‑95 (MQ=25)
                                     ataaggcgTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGATCGAT‑‑C‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAGTtttg                                                               >  1:374889/5‑100 (MQ=21)
                                       gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc                                                             <  1:2951246/100‑1 (MQ=255)
                                        cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCTGGCATTtttgcgt                                                            <  2:2359807/100‑3 (MQ=255)
                                        cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct                                                            >  1:417908/1‑100 (MQ=255)
                                         ggcgTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCAT‑‑C‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaattctgtgtt                                                           <  1:2226081/100‑11 (MQ=25)
                                         ggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGAGAATCAGGCATTtttgc                                                              >  1:3231777/1‑97 (MQ=255)
                                          gcgTTTACGCCGCATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAT‑‑TCAACTT‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCAatttggcgtt                                                           >  2:1765652/1‑94 (MQ=25)
                                          gcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgccttt                                                          <  2:2314379/100‑1 (MQ=255)
                                           cgTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGA‑‑T‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAATtttgcgtttg                                                         >  1:131119/1‑100 (MQ=17)
                                            gTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc                                                        >  2:950791/1‑100 (MQ=255)
                                               aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCAt                                                     >  1:211583/1‑100 (MQ=255)
                                                 cGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCa                                                   >  2:1242562/1‑100 (MQ=255)
                                                    cTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTc                                                >  2:2888958/1‑100 (MQ=255)
                                                        tCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGt                                            <  1:1725289/100‑1 (MQ=255)
                                                        tCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGt                                            <  1:1411125/100‑1 (MQ=255)
                                                        tCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGt                                            >  2:3387137/1‑100 (MQ=255)
                                                           ggCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATg                                         >  2:693601/1‑100 (MQ=255)
                                                             ccTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGc                                       <  1:2533937/100‑1 (MQ=255)
                                                                   aaaaTCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCtttt                                   <  2:1223838/99‑1 (MQ=255)
                                                                     aaTCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAat                                <  2:1449453/100‑1 (MQ=255)
                                                                     aaTCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAat                                <  1:2611307/100‑1 (MQ=255)
                                                                             aaaTTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGAt                        >  2:330244/1‑100 (MQ=255)
                                                                                  cAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAgaga                   <  1:1059153/100‑1 (MQ=255)
                                                                                      aCATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGtt               >  1:1541030/1‑100 (MQ=255)
                                                                                       cATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGttt              <  1:1200172/100‑1 (MQ=255)
                                                                                           gCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGa          >  1:389314/1‑100 (MQ=255)
                                                                                           gCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGa          >  1:1261276/1‑100 (MQ=255)
                                                                                            cAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGaa         >  1:2464455/1‑100 (MQ=255)
                                                                                               gAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGt       <  2:2849576/100‑1 (MQ=255)
                                                                                                aaT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTg      <  1:2216764/100‑1 (MQ=255)
                                                                                                aaT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCGTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTg      <  1:2904003/100‑1 (MQ=255)
                                                                                                 aT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGa     <  1:2317937/100‑1 (MQ=255)
                                                                                                         |                                                                                                     
TACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATGT  >  NC_000913/3705931‑3706128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: