Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,714,1631.T25.9% 272.2 / 151.1 135coding (2232/2334 nt)bisCbiotin sulfoxide reductase
Reads supporting (aligned to +/- strand):  ref base . (43/57);  new base T (21/14);  total (64/71)
Fisher's exact test for biased strand distribution p-value = 1.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 143 total aligned reads displayed.

TGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTCGTGAATG  >  NC_000913/3714064‑3714262
                                                                                                    |                                                                                                   
  aGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCcg                                                                                                   >  2:142066/1‑100 (MQ=255)
  aGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCcg                                                                                                   >  2:2369787/1‑100 (MQ=255)
  aGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCcg                                                                                                   >  1:2364896/1‑100 (MQ=255)
      ggCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCCCAGCCATTCCCCAGCCGCGAg                                                                                               >  2:427990/1‑100 (MQ=255)
      ggCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAg                                                                                               >  2:1167702/1‑100 (MQ=255)
         cGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTg                                                                                            <  1:3466606/100‑1 (MQ=255)
          ggtggtGCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGctcgcggctgg                                                                                            <  1:1533706/100‑11 (MQ=255)
           gtggtTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTggg                                                                                          >  2:2983713/1‑100 (MQ=255)
             ggtTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCTCGCGGCTGGGga                                                                                        <  1:1068115/100‑1 (MQ=255)
              gtTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGgag                                                                                       <  1:3072939/100‑1 (MQ=255)
              gtTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGgag                                                                                       <  2:589210/100‑1 (MQ=255)
               ttCAAACGCTGTAAGTGTCAGTTCCGGCCCGTTGTATTTTCCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCTCGCGGCTGGGgaat                                                                                      <  2:1002262/100‑3 (MQ=255)
               ttCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCTCGCGGCTGGGgaat                                                                                      <  2:716231/100‑3 (MQ=255)
               ttCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCTCGCGGCTGGGgaat                                                                                      <  2:2499956/100‑3 (MQ=255)
               ttCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGgaga                                                                                      >  2:700035/1‑100 (MQ=255)
               ttCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGgaga                                                                                      >  2:732915/1‑100 (MQ=255)
                tCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCTCGCGGCTGGGgaatg                                                                                     <  2:1352494/100‑4 (MQ=255)
                tCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGAt                                                                                     <  1:142064/100‑1 (MQ=255)
                 cAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGgaatgg                                                                                    <  1:396850/100‑5 (MQ=255)
                 cAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATc                                                                                    >  2:1143055/1‑100 (MQ=255)
                  aaaCGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCTCGCGGCTGGGgaatggc                                                                                   <  1:1016395/100‑6 (MQ=38)
                  aaaCGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCTCGCGGCTGGGgaatggc                                                                                   <  2:3025669/100‑6 (MQ=38)
                  aaaCGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCTCGCGGCTGGGgaatggc                                                                                   <  2:339452/100‑6 (MQ=38)
                  aaaCGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCTCGCGGCTGGGgaatggc                                                                                   <  2:1189423/100‑6 (MQ=38)
                  aaaCGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCt                                                                                   >  2:2392949/1‑100 (MQ=255)
                     cGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTg                                                                                <  2:2586271/100‑1 (MQ=255)
                      gCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTgg                                                                               <  1:681732/100‑1 (MQ=255)
                        tGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTc                                                                             >  1:2389546/1‑100 (MQ=255)
                           aaGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGc                                                                          <  1:1643022/100‑1 (MQ=255)
                            aGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCa                                                                         <  1:3406473/100‑1 (MQ=255)
                            aGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCa                                                                         <  2:3447838/100‑1 (MQ=255)
                               gtCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGt                                                                      >  1:2588188/1‑100 (MQ=255)
                                tCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGtt                                                                     >  1:1201776/1‑100 (MQ=255)
                                 cAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTc                                                                    >  2:1893320/1‑100 (MQ=255)
                                   gTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCAc                                                                  <  1:2983664/100‑1 (MQ=255)
                                      ncGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTgc                                                               >  1:2742051/2‑100 (MQ=255)
                                      ccGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTgc                                                               >  1:611415/1‑100 (MQ=255)
                                       cGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTgcg                                                              >  2:1987941/1‑100 (MQ=255)
                                        ggACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTgcgc                                                             >  1:2733732/1‑100 (MQ=255)
                                         gACCGTTGTATGTTTCCAGCGATGCCAGCGCCGTATTACCCGCACTGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGTTCAGCACGTTCAcgg                                                                <  1:1987453/96‑3 (MQ=255)
                                              cttccgatctCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGtttt                                                       <  2:3445319/91‑1 (MQ=255)
                                               tGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGttttt                                                      >  1:205924/1‑100 (MQ=255)
                                                 tATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTAc                                                    >  2:2099231/1‑100 (MQ=255)
                                                 tATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTAc                                                    >  1:1612924/1‑100 (MQ=255)
                                                   tttttCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACaa                                                  <  1:1645422/100‑1 (MQ=255)
                                                     tttCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAAt                                                >  2:1758730/1‑100 (MQ=255)
                                                      ttCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATa                                               <  2:413861/100‑1 (MQ=255)
                                                        ccAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGttttagatcggaag                                             >  1:3445266/1‑90 (MQ=255)
                                                           gCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTc                                          >  2:607930/1‑100 (MQ=255)
                                                           gCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTc                                          >  2:1036958/1‑100 (MQ=255)
                                                           gCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTc                                          >  1:52192/1‑100 (MQ=255)
                                                            ccATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCa                                         <  1:1775889/100‑1 (MQ=255)
                                                             cATGCTAGCGCCGTATTACCAGCACAGCCATTCCCCAGCCGCGGGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCg                                            <  1:2906870/96‑1 (MQ=255)
                                                                gCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCgg                                     <  2:1974566/100‑1 (MQ=255)
                                                                 gatctgcCGTATTACCCGCACAGCCATTCCCCAGACGCGAGATAAGGAGATCTTTGGTCAGCACGTTCACTGCGCCGATTATACAAATTCTGTCAGCGGt                                    <  1:2192260/95‑1 (MQ=255)
                                                                   aGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTa                                  <  2:2978054/100‑1 (MQ=255)
                                                                       ccGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATc                              <  2:2127641/100‑1 (MQ=255)
                                                                        cGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATcc                             <  1:130332/100‑1 (MQ=255)
                                                                        cGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATcc                             <  2:1353434/100‑1 (MQ=255)
                                                                        cGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATcc                             >  1:2958077/1‑100 (MQ=255)
                                                                          tATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAg                           >  1:1092510/1‑100 (MQ=255)
                                                                          tATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAg                           <  2:2514144/100‑1 (MQ=255)
                                                                             tACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATc                        <  2:892013/100‑1 (MQ=255)
                                                                               cccGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCg                      <  2:1609753/100‑1 (MQ=255)
                                                                                ccGCCCAACCATTCCCCAGCCGCGACCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCgg                     <  2:645980/100‑1 (MQ=255)
                                                                                 cGCACAGCCAGTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTGCAAATACCGTCAGCGGTTAAATCCAGATCCGGc                    <  1:2290448/100‑1 (MQ=255)
                                                                                  gCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGcc                   <  2:2859725/100‑1 (MQ=255)
                                                                                  cttccgatcttCCCCAGCTCGCGGCTGGGGAGATCTTTGGTCAGCACGTTCATTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGcc                   <  1:1672496/91‑1 (MQ=255)
                                                                                   cacaGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCa                  >  2:3001034/1‑100 (MQ=255)
                                                                                      aGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGTGGTTAAATCCAGATCCGGCCATGc               <  1:2561759/100‑1 (MQ=255)
                                                                                      aGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGc               <  1:2315681/100‑1 (MQ=255)
                                                                                       gCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGcn              <  2:2742095/100‑2 (MQ=255)
                                                                                       gCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGcc              >  1:1784886/1‑100 (MQ=255)
                                                                                       gCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGcc              >  1:218464/1‑100 (MQ=255)
                                                                                       gCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGcc              <  1:3259907/100‑1 (MQ=255)
                                                                                        ccATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGccc             >  1:2781237/1‑100 (MQ=255)
                                                                                        ccATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGccc             >  2:3196216/1‑100 (MQ=255)
                                                                                         cATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGACCCGGCCATGcccc            >  2:2204447/1‑100 (MQ=255)
                                                                                          tctCCCCAGCTCGCGGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCt           >  1:113645/3‑100 (MQ=255)
                                                                                          tctCCCCAGCTCGCGGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCt           >  2:902105/3‑100 (MQ=255)
                                                                                          tctCCCCAGCTCGCGGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCt           >  1:300385/3‑100 (MQ=255)
                                                                                           ttCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCtt          >  1:1251972/1‑100 (MQ=255)
                                                                                           ctCCCCAGCTCGCGGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGCTCCGGCCATGCCCCtt          >  1:1433951/2‑100 (MQ=255)
                                                                                           ctCCCCAGCTCGCGGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCtt          >  2:732226/2‑100 (MQ=255)
                                                                                           ctCCCCAGCTCGCGGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCtt          >  2:2725001/2‑100 (MQ=255)
                                                                                           ctCCCCAGCTCGCGGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCtt          >  2:2533740/2‑100 (MQ=255)
                                                                                           ctCCCCAGCTCGCGGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCtt          >  2:396843/2‑100 (MQ=255)
                                                                                           ctCCCCAGCTCGCGGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCtt          >  1:3402777/2‑100 (MQ=255)
                                                                                           ctCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCtt          >  1:2252094/2‑100 (MQ=255)
                                                                                            tCCCCAGCTCGCGGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTc         >  2:3070010/1‑100 (MQ=255)
                                                                                            tCCCCAGCTCGCGGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTc         >  1:547817/1‑100 (MQ=255)
                                                                                            tCCCCAGCTCGCGGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTc         >  1:363493/1‑100 (MQ=255)
                                                                                            tCCCCAGCTCGCGGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTc         >  2:2746685/1‑100 (MQ=255)
                                                                                            tCCCCAGCTCGCGGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTc         >  2:2825983/1‑100 (MQ=255)
                                                                                             ccccAGCTCGCGGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTCg        >  1:3335791/1‑100 (MQ=255)
                                                                                             ccccAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTCg        <  2:147258/100‑1 (MQ=255)
                                                                                              cccAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTCGt       >  2:450869/1‑100 (MQ=255)
                                                                                                 aGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTCGTGaa    <  1:3473318/100‑1 (MQ=255)
                                                                                                 aGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTCGTGaa    <  2:3260407/100‑1 (MQ=255)
                                                                                                  gCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTCGTGAAt   <  1:1143040/100‑1 (MQ=255)
                                                                                                    |                                                                                                   
TGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTCAGCGGTTAAATCCAGATCCGGCCATGCCCCTTCGTGAATG  >  NC_000913/3714064‑3714262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: