Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,060,2610GT25.5% 281.9 / 104.3 149intergenic (+31/‑186)typA/yihLGTP‑binding protein/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (54/57);  new base T (12/26);  total (66/83)
Fisher's exact test for biased strand distribution p-value = 8.85e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.29e-01

Only 100 of 153 total aligned reads displayed.

CGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTA  >  NC_000913/4060162‑4060360
                                                                                                   |                                                                                                    
cGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAg                                                                                                      >  2:3477386/1‑100 (MQ=255)
    ttCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGAt                                                                                                  <  2:453390/100‑1 (MQ=255)
    tccGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGAt                                                                                                  >  2:3437577/3‑100 (MQ=255)
     tCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATg                                                                                                 <  2:2235793/100‑1 (MQ=255)
     tCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCACCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATg                                                                                                 <  1:16123/100‑1 (MQ=255)
      cGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATgg                                                                                                >  2:1340044/1‑100 (MQ=255)
      cGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATga                                                                                                >  1:2235761/1‑99 (MQ=255)
       gTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGc                                                                                               <  1:1011271/100‑1 (MQ=255)
        tAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCa                                                                                              >  2:688210/1‑100 (MQ=255)
        tAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCa                                                                                              >  1:289335/1‑100 (MQ=255)
        tAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCa                                                                                              <  2:25078/100‑1 (MQ=255)
         aaaCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAg                                                                                             <  2:2917334/100‑1 (MQ=255)
          aaCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAgg                                                                                            <  2:670152/100‑1 (MQ=255)
          aaCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAgg                                                                                            <  2:1057704/100‑1 (MQ=255)
          aaCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAgg                                                                                            <  2:227287/100‑1 (MQ=255)
          aaCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAgg                                                                                            <  1:1756131/100‑1 (MQ=255)
           aCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGt                                                                                          <  2:524147/100‑1 (MQ=255)
           aCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGt                                                                                          <  1:3024854/100‑1 (MQ=255)
           aCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGt                                                                                          <  1:2569331/100‑1 (MQ=255)
           aCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGTAGGt                                                                                          >  2:1243014/1‑100 (MQ=255)
            cGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGtt                                                                                         <  1:1227902/100‑1 (MQ=255)
               cACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCCCCGAAAGACGATTAATTTCTTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGttttt                                                                                      <  1:1898127/100‑1 (MQ=38)
               cACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGttttt                                                                                      <  2:940787/100‑1 (MQ=255)
               cACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACAGAAAGACGATTAATTTCGTGTTTTAGTCCTAAAAAACCTGCCAGCGATGGCAGGttttt                                                                                      >  1:851857/1‑100 (MQ=255)
                  cTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGttttttat                                                                                   <  1:2391816/100‑3 (MQ=255)
                  cTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGttttttat                                                                                   <  1:134661/100‑3 (MQ=255)
                  cTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGttttttat                                                                                   <  2:1920507/100‑3 (MQ=255)
                  cTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGttttttat                                                                                   <  1:3467808/100‑3 (MQ=255)
                  cTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtttttttt                                                                                   >  2:3268657/1‑100 (MQ=255)
                  atctcGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGttttttat                                                                                   <  1:1633121/96‑3 (MQ=38)
                   tgccGGAAAACGATCGTGGCCGCGCCAACCTCGCACCGAAAGACGCTTCATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAAGtttttta                                                                                    <  1:394802/95‑2 (MQ=37)
                   tGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttt                                                                                  <  2:882593/100‑1 (MQ=255)
                    gACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTg                                                                                 <  2:1046795/100‑1 (MQ=255)
                     aCGGAAAACGATCGTCGCCGCGCCAACCGCGGACCGAAAGACGATTAATTTCTTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGttttttatgac                                                                                <  2:1642731/100‑6 (MQ=37)
                     aCGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGGCGATTAATTTCGTTTTTTAGCCATAAAAAGCCTGCCAGCGATGGTAGGttttttttgcc                                                                                <  1:2144635/100‑4 (MQ=255)
                     aCGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGc                                                                                <  2:3336868/100‑1 (MQ=255)
                     aCGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGc                                                                                <  2:1974986/100‑1 (MQ=255)
                      cGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGttttttatagat                                                                               >  2:1633143/1‑94 (MQ=38)
                      cGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGcc                                                                               <  2:2166384/100‑1 (MQ=255)
                      cGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGcc                                                                               >  2:3489/1‑100 (MQ=255)
                      cGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGcc                                                                               <  1:2980931/100‑1 (MQ=255)
                      cGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGcc                                                                               <  1:1676851/100‑1 (MQ=255)
                       ggAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCt                                                                              <  1:668189/100‑1 (MQ=255)
                       ggAAAACGATCGACGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCt                                                                              >  2:2290548/1‑100 (MQ=255)
                        gAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTg                                                                             >  2:164369/1‑100 (MQ=255)
                        gAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTg                                                                             >  2:85375/1‑100 (MQ=255)
                          aaaCGATCGTCGCAGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTTCATGt                                                                            >  1:2806614/1‑99 (MQ=255)
                             cGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTg                                                                        <  2:2351113/100‑1 (MQ=255)
                             cGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTg                                                                        <  2:1436619/100‑1 (MQ=255)
                               aTCGTCGCCGCGCCAACCGCGCACCGAAAGACGAGTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTGGCCTGTCGTGAg                                                                      <  1:1519939/100‑1 (MQ=255)
                                tcgtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAgc                                                                     <  1:1936357/100‑1 (MQ=255)
                                tcgtcgCCGCCCCAACCGCGCACCAAAAGACGATTAATTTCGTTTTTTAGTCATAAACAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAgc                                                                     <  1:1666799/100‑1 (MQ=255)
                                  gtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAgcgc                                                                   <  2:3028235/100‑1 (MQ=255)
                                    cgccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGcac                                                                 >  1:3362834/1‑100 (MQ=255)
                                             aaCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCg                                                        <  2:1292163/100‑1 (MQ=255)
                                             aaCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCg                                                        <  2:3298048/100‑1 (MQ=255)
                                               ccGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAg                                                      <  2:2607291/100‑1 (MQ=255)
                                               ccGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAg                                                      <  1:2252275/100‑1 (MQ=255)
                                                      ccGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtcaga                                               >  2:2998290/1‑97 (MQ=255)
                                                       cGAAAGACGATTTATTTCGTTTTTTAGTCATGAAAAAGCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTccc                                              <  1:2349164/100‑1 (MQ=255)
                                                         aaaGACGTTTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCtt                                            >  2:1827466/1‑100 (MQ=255)
                                                           aGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtat                                          <  2:1100070/100‑1 (MQ=255)
                                                            gACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtata                                         <  1:2752838/100‑1 (MQ=255)
                                                             aCGATTAATTTCGTTTTTTAGTCATAAAATACCTGCCAGCGATGGCAGGTTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtata                                         <  1:3223463/100‑1 (MQ=255)
                                                               gATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTTCCTTATAAg                                       >  1:2257513/1‑99 (MQ=255)
                                                                aTTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTc                                     <  2:2497693/100‑1 (MQ=255)
                                                                aTTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTc                                     >  2:3123000/1‑100 (MQ=255)
                                                                 ttAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCt                                    >  2:656617/1‑100 (MQ=255)
                                                                    aTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgtg                                 >  2:121682/1‑100 (MQ=255)
                                                                      ttCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGt                               >  2:3436833/1‑100 (MQ=255)
                                                                       tCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGtt                              <  1:1232001/100‑1 (MQ=255)
                                                                        cGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCGCACCAATGCGAGTATCTCCCTTATAAGTCTGTGGtt                              <  1:765244/99‑1 (MQ=255)
                                                                            ttttAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTc                         <  1:2001129/100‑1 (MQ=255)
                                                                            ttttAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTc                         >  1:650168/1‑100 (MQ=255)
                                                                                aGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATa                     >  2:2822956/1‑100 (MQ=255)
                                                                                  tCATAGAGAGCCGGCCAGCGAGGGGAGGTTTTTTTTTGCCTGTCGTGGGCGCACGCCGATGCCAGTAGCTCCCTGATAAGTCTGTGGGTACGTCAATa                     <  1:2746730/98‑1 (MQ=255)
                                                                                  caaaaaaaaaCCTGCCATCGCTGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATaga                   >  2:1069219/5‑100 (MQ=38)
                                                                                  caaaaaaaaaCCTGCCATCGCTGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATaga                   >  2:1398153/5‑100 (MQ=38)
                                                                                   cATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTTCCTGTCTTTTGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagag                  >  1:2516841/1‑100 (MQ=255)
                                                                                    aaaaaaaaCCTGCCATCGCTGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagaga                 >  1:468565/3‑100 (MQ=255)
                                                                                    aTAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagaga                 <  2:778917/100‑1 (MQ=255)
                                                                                     tAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTTCCTGTCGTGAGCGCACACCAACGCGCGTATCTCCCTTATAAGTCTGTGCTTACGTCAATcga                   >  1:13035/1‑97 (MQ=255)
                                                                                     tAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagagag                >  1:2765410/1‑100 (MQ=255)
                                                                                     tAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagagag                >  2:2399449/1‑100 (MQ=255)
                                                                                     aaaaaaaCCTGCCATCGCTGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagagag                >  2:2818524/2‑100 (MQ=255)
                                                                                      aaaaaaCCTGCCATCGCTGGCAGGTTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagagag                >  2:242436/1‑100 (MQ=38)
                                                                                      aaaaaaCCTGCCAGCGGTGGCAGGTATTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCAGTGGTTACGTCATTaga                   >  1:3003241/1‑96 (MQ=255)
                                                                                      aaaaaaCCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGc               <  2:2752099/100‑1 (MQ=255)
                                                                                      aaaaaaCCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGc               >  2:1759352/1‑100 (MQ=255)
                                                                                        aaaaCCTGCCATCGCTGGCAGGTTTTTTTTTTTTTTTTTTTTGCTTACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTTCGTCTATAGAGTGCtt             >  1:153868/1‑100 (MQ=16)
                                                                                        aaaaCCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCtt             >  2:2907873/1‑100 (MQ=255)
                                                                                         aaaCCTGCCATCGCTGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCttt            >  2:297006/1‑100 (MQ=255)
                                                                                           aCCTGCCATCGCTGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGt          >  2:3144401/1‑100 (MQ=255)
                                                                                            ccTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTc         <  2:3072752/100‑1 (MQ=255)
                                                                                            ccTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTc         <  2:1562929/100‑1 (MQ=255)
                                                                                             cTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTca        >  2:1383505/1‑100 (MQ=255)
                                                                                              tGCCATCGCTGGCAGGTTTTTTTTTGCCTGTCGTGAGAGCACACCACTGCGAGTATCTCCCTTCTAAGACTGTGGTTACGTCACTAGCGCGCTTTGTca        >  2:2027475/1‑99 (MQ=255)
                                                                                              tGCCAGCGATGGCAGGTTTTTTTTTTTTTTTTTTTAACGCACACCAATGCGAGTATATCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTTTcac       >  1:105867/1‑100 (MQ=255)
                                                                                                  aGCGATGGCAGGTCTTTTTTTTTCTGAACTGAGCGCACACCACTCCGATGATCTCCCATTTTAGTGAATGAGTAAGTCAGCAGAGAGCTTTGTcacatt    >  1:1733968/1‑97 (MQ=255)
                                                                                                   gCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTa  <  2:2760437/100‑1 (MQ=255)
                                                                                                   |                                                                                                    
CGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTA  >  NC_000913/4060162‑4060360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: