Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 179,442 A→T H69L (CAC→CTC)  dgt → deoxyguanosine triphosphate triphosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913179,4420AT98.2% 345.1 / NA 112H69L (CAC→CTC) dgtdeoxyguanosine triphosphate triphosphohydrolase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  major base T (34/74);  minor base C (0/2);  total (35/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

GGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCACTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACTGGAAGCATACGGCCTGGATG  >  NC_000913/179346‑179537
                                                                                                 |                                                                                               
ggCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCtctc                                                                                              >  1:1136718/1‑100 (MQ=255)
 gCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCg                                                                                             <  2:2519382/100‑1 (MQ=255)
   gTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGAt                                                                                           <  2:1253814/100‑1 (MQ=255)
   gTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGAt                                                                                           >  1:2425752/1‑100 (MQ=255)
   gTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGAt                                                                                           >  1:1804298/1‑100 (MQ=255)
    tATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATg                                                                                          <  2:937679/100‑1 (MQ=255)
    tATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATg                                                                                          <  1:2859626/100‑1 (MQ=255)
    tATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATg                                                                                          <  1:2715465/100‑1 (MQ=255)
     atcatcAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATgg                                                                                         <  2:289791/100‑1 (MQ=255)
     atcatcAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATgg                                                                                         <  1:843717/100‑1 (MQ=255)
      tcatcaACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGa                                                                                        <  2:941911/100‑1 (MQ=255)
      tcatcaACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGa                                                                                        <  2:1804323/100‑1 (MQ=255)
       catcaACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGaa                                                                                       >  1:2460825/1‑100 (MQ=255)
        atcaACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAg                                                                                      >  2:2093794/1‑100 (MQ=255)
        atcaACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAg                                                                                      <  1:2258073/100‑1 (MQ=255)
        atcaACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAg                                                                                      <  2:2743605/100‑1 (MQ=255)
           aaCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTcc                                                                                   <  2:3216994/100‑1 (MQ=255)
            aCTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCca                                                                                  >  2:191236/1‑100 (MQ=255)
              tctcCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCcagc                                                                                <  2:596908/100‑1 (MQ=255)
                 ccGGCACTTCGGCCTCTGCAACAACTGGCCGCGGGTTGTTCCCCGGCGCGAAATGCCCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCcagcag                                                                              <  1:2676183/98‑1 (MQ=255)
                 ccGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAgg                                                                             >  1:1217799/1‑100 (MQ=255)
                 ccGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAgg                                                                             <  2:1309367/100‑1 (MQ=255)
                 ccGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAgg                                                                             <  2:3051071/100‑1 (MQ=255)
                  cGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGt                                                                            >  2:2662948/1‑100 (MQ=255)
                   ggCAATTCGTCGTCTGCAACAAACGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCGAGCAGGTg                                                                           >  1:1883991/1‑100 (MQ=255)
                   ggCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTg                                                                           <  2:1910010/100‑1 (MQ=255)
                     cAATTCGTCGTCTGCAACAAAAGACCCAGGTTGTTCCACTGGAGCGCAATGCAGCCGTGCGGACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTggg                                                                         <  1:674994/100‑1 (MQ=255)
                      aaTTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTgggg                                                                        >  2:387889/1‑100 (MQ=255)
                      aaTTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTgggg                                                                        <  2:1553450/100‑1 (MQ=255)
                       aTTCGTCGTCTGCAACAAACGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTgggg                                                                        >  1:1586374/1‑99 (MQ=255)
                       aTTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGgc                                                                       <  2:1094989/100‑1 (MQ=255)
                         tcgtcgTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGgcgc                                                                     <  2:2131783/100‑1 (MQ=255)
                         tcgtcgTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGgcgc                                                                     <  1:937201/100‑1 (MQ=255)
                          cgtcgtCTGCAACCAAAGACCCAGGTTTTTCCACTGGAGCGCGATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCt                                                                    <  1:3167013/100‑1 (MQ=255)
                          cgtcgtCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCt                                                                    >  2:264139/1‑100 (MQ=255)
                           gtcgtcTGCAACAAAAGACCCAGGTTTTTCCACCGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTa                                                                   <  1:3354190/100‑1 (MQ=255)
                           gcggtcTGCAACAAAAGACCCAGTTTTTTCCACTTGAGCGCATTGCCGACGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGgcg                                                                      <  1:553013/94‑1 (MQ=255)
                           gacgtcTGGAACAAGAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCt                                                                    <  1:1805174/97‑1 (MQ=255)
                            tcgtcTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCACTCGATGGAAGTCCAGCAGGTGGGGCGCTAc                                                                  >  2:1569508/1‑100 (MQ=255)
                               tcTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATc                                                               >  1:335237/1‑100 (MQ=255)
                                cTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCg                                                              <  2:3064531/100‑1 (MQ=255)
                                 tGCAACGAAAGACCCAGGTTTTGGCACTGCATCGCTATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGgcg                                                                      <  1:552713/91‑1 (MQ=255)
                                   caacaaAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCa                                                           >  1:1933003/1‑100 (MQ=255)
                                   caacaaAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCa                                                           >  2:1503191/1‑100 (MQ=255)
                                    aacaaAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCACTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCaa                                                          <  1:568506/100‑1 (MQ=255)
                                     acaaAAGACCCAGGTTTTTCCACTGGGGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCa                                                           <  1:3163653/98‑1 (MQ=255)
                                     acaaAAGACCCAGGTTTTTCCACTGGAGCACAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCaaa                                                         <  1:2355163/100‑1 (MQ=255)
                                      caaAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCaaa                                                         >  2:2874777/1‑99 (MQ=255)
                                      caaAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAg                                                        <  2:1933032/100‑1 (MQ=255)
                                       aaaaGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACAGCGCCAAAg                                                        <  1:1328883/99‑1 (MQ=255)
                                          aGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAAt                                                    <  1:1503169/100‑1 (MQ=255)
                                          aGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAAt                                                    <  1:507698/100‑1 (MQ=255)
                                           gACCCAGGTTTTTCCTCTGGACCGCAATGCCGCCGTGCGGACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAAt                                                    <  1:1731253/99‑1 (MQ=255)
                                            aCCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAAttt                                                  <  2:2066628/100‑1 (MQ=255)
                                             nccAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAAtttt                                                 >  1:3090355/2‑100 (MQ=255)
                                             cccAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCACAGAAAtttt                                                 >  1:917416/1‑100 (MQ=255)
                                              ccAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTa                                                <  2:3105178/100‑1 (MQ=255)
                                                 aGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGAACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAg                                              <  1:694605/100‑1 (MQ=255)
                                                 aGGGTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAg                                              <  1:2684648/100‑1 (MQ=255)
                                                      tttCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTc                                         <  2:2756875/100‑1 (MQ=255)
                                                       tttcACTCGAGCGCAATGCCGCCGTTCGTACGCGTCTTACCCTCTCGATGGAAGTCCAGCACGCGGGGCGCTTGATCGCCAACGAAATTTTAAGCCGTCt                                        <  2:1680138/97‑1 (MQ=255)
                                                        tCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTg                                       <  2:1650752/100‑1 (MQ=255)
                                                        tCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGGGGGGCGCTACATCGCCAAAGAAATTGTAAGCCGTCTg                                       >  2:1025936/1‑100 (MQ=255)
                                                         ccACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGa                                      <  2:366559/100‑1 (MQ=255)
                                                          cACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGaa                                     >  2:2702333/1‑100 (MQ=255)
                                                          atcTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGa                                      <  1:187490/97‑1 (MQ=255)
                                                            cTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAag                                   >  2:190152/1‑100 (MQ=255)
                                                              ggAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGaagatc                                 >  2:187492/1‑96 (MQ=255)
                                                                aGCGCAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCt                               <  1:3193964/100‑1 (MQ=255)
                                                                 gcgcAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCtt                              >  2:2683066/1‑100 (MQ=255)
                                                                 gcgcAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCtt                              >  2:3242543/1‑100 (MQ=255)
                                                                 gcgcAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCtt                              >  2:822764/1‑100 (MQ=255)
                                                                  cgcAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTa                             <  2:2462261/100‑1 (MQ=255)
                                                                   gcAATGCCGCCGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTaa                            <  2:577156/100‑1 (MQ=255)
                                                                      aTGCCGCCGGGCGCACGCGTATTACCCCCCCGATAGAAGTCCAGCAGGTGCGACGCTACATCGCAAAAGAAATGTTAAGCCGTCTGAAAGAGCTTAAAtt                         <  1:2334337/100‑1 (MQ=255)
                                                                        gccgccGTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTAc                       >  2:237946/1‑100 (MQ=255)
                                                                         ccgccgTGCGCACGCGTCTTACCCTCTCGATGGAAGTGCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACt                      >  1:1863854/1‑100 (MQ=255)
                                                                          cgccgTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACTg                     <  2:2604790/100‑1 (MQ=255)
                                                                          cgccgTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACTg                     <  1:191234/100‑1 (MQ=255)
                                                                          cgccgTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACTg                     <  2:1534282/100‑1 (MQ=255)
                                                                           gccgTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACt                      <  1:1235262/98‑1 (MQ=255)
                                                                            ccgTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACTGGa                   <  2:1557472/100‑1 (MQ=255)
                                                                              gTGCGCACGCGTCTTACCCTCTCGATGGATGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTCTAAGCCGTCTGAAAGAGCTTAAATTACTGGAAg                 <  1:2093763/100‑1 (MQ=255)
                                                                              gTGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACTGGAAg                 <  1:1767654/100‑1 (MQ=255)
                                                                               tGCGCACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACTGGAAGc                <  1:255502/100‑1 (MQ=255)
                                                                                gcgcACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACTGGAAGCa               <  2:2689452/100‑1 (MQ=255)
                                                                                gcgcACGCGTCTTACCATCTCGATGGCAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGGCTGAAAGAGCTTAAATTACTGGAAGc                <  1:2946317/99‑1 (MQ=255)
                                                                                 cgcACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACTGGAAGCAt              >  1:649170/1‑100 (MQ=255)
                                                                                 cgcACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACTGGAAGCAt              <  2:1231441/100‑1 (MQ=255)
                                                                                  gcACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACTGGAAGCAt              <  1:1598640/99‑1 (MQ=255)
                                                                                   cACGCGTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACTGGAAGCATa             <  1:178547/99‑1 (MQ=255)
                                                                                       cgTCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACTGGAAGCATACGGcc        <  2:2529737/100‑1 (MQ=255)
                                                                                         tCTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACTGGAAGCATACGGCCTg      >  2:73331/1‑100 (MQ=255)
                                                                                          cTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACTGGAAGCATACGGCCTgg     <  1:264135/100‑1 (MQ=255)
                                                                                          cTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACTGGAAGCATACGGCCTgg     <  2:410778/100‑1 (MQ=255)
                                                                                          cTTACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACTGGAAGCATACGGCCTgg     <  2:2757790/100‑1 (MQ=255)
                                                                                            tACCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACTGGAAGCATACGGCCTGGAt   >  1:200553/1‑100 (MQ=255)
                                                                                             aCCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACTGGAAGCATACGGCCTGGATg  >  2:906627/1‑100 (MQ=255)
                                                                                             aCCCTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACTGGAAGCATACGGCCTGGATg  >  1:1001514/1‑100 (MQ=255)
                                                                                              cccTCTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACTGGAAGCATACGGCCTGGATg  <  1:2325099/99‑1 (MQ=255)
                                                                                                 |                                                                                               
GGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCGTGCGCACGCGTCTTACCCACTCGATGGAAGTCCAGCAGGTGGGGCGCTACATCGCCAAAGAAATTTTAAGCCGTCTGAAAGAGCTTAAATTACTGGAAGCATACGGCCTGGATG  >  NC_000913/179346‑179537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: