Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,235,656 T→C I131T (ATC→ACC)  preA → dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,235,6560TC99.3% 490.7 / ‑5.0 141I131T (ATC→ACC) preAdihydropyrimidine dehydrogenase, NADH‑dependent, subunit C
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (69/71);  total (70/71)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

Only 100 of 141 total aligned reads displayed.

ACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGT  >  NC_000913/2235560‑2235752
                                                                                                |                                                                                                
aCCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGa                                                                                               >  1:2191641/1‑100 (MQ=255)
aCCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGa                                                                                               >  2:610366/1‑100 (MQ=255)
aCCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGa                                                                                               >  1:1673862/1‑100 (MQ=255)
aCCCGGACAAAGTATTGATCGCTTCAATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGa                                                                                               >  2:405545/1‑100 (MQ=255)
 cccGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAg                                                                                              <  1:740801/100‑1 (MQ=255)
   cGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAgtg                                                                                            <  1:2622737/100‑1 (MQ=255)
       cAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAAc                                                                                        <  1:3322808/100‑1 (MQ=255)
        aaaGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACt                                                                                       <  2:2681735/100‑1 (MQ=255)
        aaaGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACt                                                                                       <  2:1993072/100‑1 (MQ=255)
           gTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTtct                                                                                    >  2:129816/1‑100 (MQ=255)
            tATTGATCGCTTCGATCATGGGGGAAAATGTGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTtctc                                                                                   <  2:2267430/100‑1 (MQ=255)
             aTTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTcc                                                                                  <  2:3407234/100‑1 (MQ=255)
             aTTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTcc                                                                                  <  2:2314452/100‑1 (MQ=255)
             aTTGATCGCTTCGATCATGGGGGAAAAGGAGCAGCAATGGGGGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTcc                                                                                  <  1:2072076/100‑1 (MQ=255)
                gcgcGCTTCGATCATGGGGGAAAATGGGCAGCAATGGGAGGAGCGGGCGCGCGTTGTGCAATAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGt                                                                               >  1:2026641/4‑100 (MQ=255)
                gATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGt                                                                               >  2:1909092/1‑100 (MQ=255)
                 aTCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGGGTAACTTCTCCTGTc                                                                              >  2:3423063/1‑100 (MQ=255)
                  tCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTcc                                                                             <  2:2975708/100‑1 (MQ=255)
                   cGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGCGTGTAACTTCTCCTATCCg                                                                            >  2:3250264/1‑100 (MQ=255)
                   cGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTcc                                                                             <  1:1033764/99‑1 (MQ=255)
                   cGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCg                                                                            <  2:3348323/100‑1 (MQ=255)
                     cTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCa                                                                          >  2:1498414/1‑100 (MQ=255)
                     cTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCa                                                                          <  2:1913629/100‑1 (MQ=255)
                     cTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCa                                                                          >  1:1329837/1‑100 (MQ=255)
                     cTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCa                                                                          >  2:2579685/1‑100 (MQ=255)
                     cTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCa                                                                          <  1:1909064/100‑1 (MQ=255)
                     cTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGGcc                                                                             >  1:2072416/1‑97 (MQ=255)
                       tCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCaaa                                                                        >  2:2633339/1‑100 (MQ=255)
                         gATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATg                                                                      >  2:2494645/1‑100 (MQ=255)
                         gATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATg                                                                      <  1:3150400/100‑1 (MQ=255)
                          aTCATGGGGCAAAATGAGCAGCAATGGGAGAAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTACGCAAATGa                                                                     >  1:836172/1‑100 (MQ=255)
                            cATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACt                                                                   >  2:2299147/1‑100 (MQ=255)
                            cATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACt                                                                   <  1:2862111/100‑1 (MQ=255)
                             aTGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACtt                                                                  <  1:1288142/100‑1 (MQ=255)
                                gggtAAAATGAGCAGCAATGGCAGGTGCTAGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTtctc                                                               >  1:2372921/1‑100 (MQ=255)
                                ggggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTtctc                                                               >  1:2010310/1‑100 (MQ=255)
                                ggggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTtctc                                                               >  2:639600/1‑100 (MQ=255)
                                ggggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTtctc                                                               >  1:875624/1‑100 (MQ=255)
                                ggggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTtctc                                                               >  2:2796442/1‑100 (MQ=255)
                                 gggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCa                                                              <  1:50088/100‑1 (MQ=255)
                                 gggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCa                                                              <  1:405540/100‑1 (MQ=255)
                                 gggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCa                                                              >  1:245747/1‑100 (MQ=255)
                                      aaTGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGa                                                         <  2:725298/100‑1 (MQ=255)
                                       aTGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGAt                                                        <  1:1799661/100‑1 (MQ=255)
                                          agcagcAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATggg                                                     <  2:2829791/100‑1 (MQ=255)
                                          agcagcAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATggg                                                     >  1:2792871/1‑100 (MQ=255)
                                             agcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAg                                                  <  1:402839/100‑1 (MQ=255)
                                             agcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAg                                                  >  1:2334373/1‑100 (MQ=255)
                                             agcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAg                                                  >  1:1776815/1‑100 (MQ=255)
                                              gcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGc                                                 <  1:1338754/100‑1 (MQ=255)
                                               caATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTGTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCg                                                <  1:1866780/100‑1 (MQ=255)
                                               caATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCg                                                <  2:49261/100‑1 (MQ=255)
                                               caATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCg                                                <  1:2657848/100‑1 (MQ=255)
                                                 aTGGGAGGAGCTGGCGCGCCTGGTGCAAGCAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCACATGACTTCTCATGCGATGGGTAGCGAt                                              >  1:2194769/1‑100 (MQ=255)
                                                 aTGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGAt                                              <  2:1673885/100‑1 (MQ=255)
                                                    ngaggagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGAATTCTCATGCGATGGGTAGCGATg                                             >  1:1493778/2‑98 (MQ=255)
                                                    ggaggaGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGGTATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTc                                           >  1:1556658/1‑100 (MQ=255)
                                                    ggaggaGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTc                                           >  1:1948979/1‑100 (MQ=255)
                                                      aggagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCgn                                         <  2:3293676/100‑2 (MQ=255)
                                                      aggagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCgg                                         >  1:2472772/1‑100 (MQ=255)
                                                      aggagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCgg                                         <  2:2930819/100‑1 (MQ=255)
                                                      aggagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCgg                                         <  1:1117796/100‑1 (MQ=255)
                                                      aggagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCgg                                         >  1:3419100/1‑100 (MQ=255)
                                                       ggagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCggg                                        <  1:926953/100‑1 (MQ=255)
                                                       ggagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCggg                                        >  1:669050/1‑100 (MQ=255)
                                                        gagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGc                                       <  2:1021314/100‑1 (MQ=255)
                                                        gagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGc                                       <  1:2506700/100‑1 (MQ=255)
                                                        gagCGGTCGCGCCTGGTTCATGAAGCGGGCGCGGATATTACCGAGTTTAACTGCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGc                                       <  2:2026670/100‑1 (MQ=255)
                                                         agCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCa                                      >  2:251206/1‑100 (MQ=255)
                                                            tGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAg                                   >  2:3122897/1‑100 (MQ=255)
                                                             tccgcgcCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGc                                  >  2:2865332/3‑100 (MQ=255)
                                                             ggCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGc                                  >  2:2745672/1‑100 (MQ=255)
                                                              gcgcgcCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGGTGTCGGGCAAAGcc                                 <  2:404568/100‑1 (MQ=255)
                                                               cgcgcCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTGGGGCAAAGccc                                >  1:1676825/1‑100 (MQ=255)
                                                               cgcgcCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGccc                                <  1:1222205/100‑1 (MQ=255)
                                                                 cgcCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCgg                              <  2:2472805/100‑1 (MQ=255)
                                                                  gcCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGa                             >  2:2673855/1‑100 (MQ=255)
                                                                   ccTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAg                            <  2:301802/100‑1 (MQ=255)
                                                                       gTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCGTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTGGGGCAAAGCCCGGAGCTgg                        >  1:3023678/1‑100 (MQ=255)
                                                                       gTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTgg                        >  2:323200/1‑100 (MQ=255)
                                                                        tGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGt                       <  2:2839839/100‑1 (MQ=255)
                                                                         gCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGt                       <  1:1311838/99‑1 (MQ=255)
                                                                         gCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTa                      >  2:1931636/1‑100 (MQ=255)
                                                                          cAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAg                     >  1:3256301/1‑100 (MQ=255)
                                                                          cAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAg                     <  1:570096/100‑1 (MQ=255)
                                                                          cAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAg                     <  1:338583/100‑1 (MQ=255)
                                                                           aagaagCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAg                     >  2:1047404/1‑99 (MQ=255)
                                                                            agaagCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGaa                   >  1:3160723/1‑100 (MQ=255)
                                                                             gaagCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGaaa                  <  2:2186684/100‑1 (MQ=255)
                                                                                  tGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAatat             <  1:1644257/100‑1 (MQ=255)
                                                                                   ggCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATAtt            >  1:889867/1‑100 (MQ=255)
                                                                                   ggCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATAtt            >  1:2880773/1‑100 (MQ=255)
                                                                                    gcgcGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTg           <  1:1110267/100‑1 (MQ=255)
                                                                                    gcgcGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTg           >  1:3238840/1‑100 (MQ=255)
                                                                                     cgcgGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGt          <  2:872105/100‑1 (MQ=255)
                                                                                        ggATATGACCGAGTGTAACGTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCgg       <  2:1329856/100‑1 (MQ=255)
                                                                                         gATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCCGGTAGAAAAATATTGTc         >  1:2990994/1‑97 (MQ=255)
                                                                                          atatGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGCTGTCGGGCAATGCCCGGAGCTGGTAGAATAATATTGTCGGGc     <  2:2346524/100‑1 (MQ=255)
                                                                                          atatGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGc     <  1:610359/100‑1 (MQ=255)
                                                                                             tGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGt  <  1:2172410/100‑1 (MQ=255)
                                                                                                |                                                                                                
ACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGT  >  NC_000913/2235560‑2235752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: