Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,250,210 T→A V165E (GTG→GAG)  yeiH → UPF0324 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,250,2100TA99.3% 484.7 / ‑4.8 142V165E (GTG→GAG) yeiHUPF0324 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (72/69);  total (73/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.02e-01

Only 100 of 142 total aligned reads displayed.

CCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACTTT  >  NC_000913/2250114‑2250306
                                                                                                |                                                                                                
    cTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGtt                                                                                           >  1:908326/1‑100 (MQ=255)
     tGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTg                                                                                          <  2:898595/100‑1 (MQ=255)
     tGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTg                                                                                          <  1:1288151/100‑1 (MQ=255)
      ggTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTg                                                                                          >  1:3023867/1‑99 (MQ=255)
      ggTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGc                                                                                         >  1:1069079/1‑100 (MQ=255)
      ggTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGc                                                                                         >  1:3232996/1‑100 (MQ=255)
       gTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCa                                                                                        <  2:2708277/100‑1 (MQ=255)
        ttGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCaa                                                                                       >  2:416876/1‑100 (MQ=255)
        ttGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCaa                                                                                       >  2:3307161/1‑100 (MQ=255)
         tGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAAc                                                                                      <  1:3037635/100‑1 (MQ=255)
         tGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCCGTTGCAAc                                                                                      >  1:2240560/1‑100 (MQ=255)
          gATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAAcc                                                                                     >  1:2731757/1‑100 (MQ=255)
              ggTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCgttg                                                                                 >  2:2962064/1‑100 (MQ=255)
              ggTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCgttg                                                                                 >  2:1534219/1‑100 (MQ=255)
               gTGCGGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCgttgt                                                                                <  1:348198/100‑1 (MQ=255)
               gTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCgttgt                                                                                <  1:2959239/100‑1 (MQ=255)
                  ccGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTAt                                                                             >  2:1049938/1‑100 (MQ=255)
                      tAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTc                                                                         <  2:653300/100‑1 (MQ=255)
                         cAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCggg                                                                      <  1:430446/100‑1 (MQ=255)
                           gTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGAc                                                                    >  1:3445219/1‑100 (MQ=255)
                             aTCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCg                                                                  <  2:199055/100‑1 (MQ=255)
                             aTCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCg                                                                  >  1:2678464/1‑100 (MQ=255)
                             aTCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCg                                                                  <  2:1799385/100‑1 (MQ=255)
                              tCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGt                                                                 >  2:1166349/1‑100 (MQ=255)
                               cTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCAGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATATTCGGGAGCGTc                                                                >  1:1138166/1‑100 (MQ=255)
                                  tggtgCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTcgcg                                                             >  1:765875/1‑100 (MQ=255)
                                  tggtgCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTcgcg                                                             <  2:2904039/100‑1 (MQ=255)
                                    gtgCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGAt                                                           <  1:3323717/100‑1 (MQ=255)
                                     tgctgcCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGAtt                                                          >  2:2304945/1‑100 (MQ=255)
                                       gatctgcgGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGAtttt                                                        <  1:1640631/95‑1 (MQ=255)
                                       ctgcCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGAtttt                                                        <  1:233251/100‑1 (MQ=255)
                                        tgcCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTc                                                       >  1:908286/1‑100 (MQ=255)
                                        cttccgatctcTGGCGACTGAGCCGTTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTc                                                       <  1:1257147/90‑1 (MQ=255)
                                         gcCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTcc                                                      <  2:1197614/100‑1 (MQ=255)
                                         gcCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTcc                                                      <  2:2730608/100‑1 (MQ=255)
                                         gcCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTcc                                                      <  2:2731800/100‑1 (MQ=255)
                                          ccGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCct                                                     <  1:744037/100‑1 (MQ=255)
                                          ccGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCct                                                     >  2:2534649/1‑100 (MQ=255)
                                            gcgGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGAttttagatc                                                   >  2:1640653/1‑95 (MQ=255)
                                            gcgGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCctct                                                   >  2:543153/1‑100 (MQ=255)
                                            gcgGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCctct                                                   <  2:908338/100‑1 (MQ=255)
                                               gTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTAcc                                                >  1:2320245/1‑100 (MQ=255)
                                               gTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTAcc                                                <  1:2084451/100‑1 (MQ=255)
                                                tACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTAccc                                               <  1:2962016/100‑1 (MQ=255)
                                                 aCTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCg                                              >  1:963617/1‑100 (MQ=255)
                                                   tGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGc                                            <  2:2816220/100‑1 (MQ=255)
                                                    ggCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCg                                           >  1:657523/1‑100 (MQ=255)
                                                    ggCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCg                                           >  1:1426340/1‑100 (MQ=255)
                                                    ggCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCg                                           >  2:66088/1‑100 (MQ=255)
                                                      cGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGat                                         <  1:2717048/100‑1 (MQ=255)
                                                      cGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGat                                         <  2:765886/100‑1 (MQ=255)
                                                      cGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGat                                         <  2:766498/100‑1 (MQ=255)
                                                       gACTGAGCCGGTAGTGCAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGata                                        <  1:2030832/100‑1 (MQ=255)
                                                           gAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATc                                    >  2:1601003/1‑100 (MQ=255)
                                                           gAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATc                                    >  2:1958470/1‑100 (MQ=255)
                                                           gAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTCCCTCTCCCCCGCGATATATc                                    >  1:270363/1‑100 (MQ=255)
                                                             gCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCg                                  <  2:2791109/100‑1 (MQ=255)
                                                             gCCGGTAGTGAAAGCGGAAGCCAGTAAAGGACCCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATc                                    <  1:2225/98‑1 (MQ=255)
                                                              ccGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGc                                 <  2:1190981/100‑1 (MQ=255)
                                                               cGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCt                                <  2:2200252/100‑1 (MQ=255)
                                                               cGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCt                                <  1:878923/100‑1 (MQ=255)
                                                                ggTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTg                               >  1:947922/1‑100 (MQ=255)
                                                                ggTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTg                               >  2:1960074/1‑100 (MQ=255)
                                                                  tAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGAt                             <  2:2127229/100‑1 (MQ=255)
                                                                  tAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGAt                             <  2:378179/100‑1 (MQ=255)
                                                                   aGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATg                            >  2:1518591/1‑100 (MQ=255)
                                                                    gTGAAAGTGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGt                           >  2:2534065/1‑100 (MQ=255)
                                                                     tGAAAGCGGAAGCCAGTAAGGTAACCGAGGCTGTTGCAACCGGTGTTATCTTCGGGACCGTCGCGATTTTCATCTACCCGGCGATATATCCGCTGATGtc                          >  1:1218984/1‑100 (MQ=255)
                                                                     tGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGtc                          >  1:1067243/1‑100 (MQ=255)
                                                                     tGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGtc                          >  2:1347842/1‑100 (MQ=255)
                                                                      gAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGtct                         >  2:2419378/1‑100 (MQ=255)
                                                                       aaaGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGtctc                        <  1:2454355/100‑1 (MQ=255)
                                                                       aaaGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGtctc                        <  1:2454356/100‑1 (MQ=255)
                                                                       aaaGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGtctc                        <  1:663685/100‑1 (MQ=255)
                                                                         aGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCaa                      <  2:853445/100‑1 (MQ=255)
                                                                          gCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAAt                     <  1:55115/100‑1 (MQ=255)
                                                                          gCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAAt                     <  2:2658249/100‑1 (MQ=255)
                                                                          gCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAAt                     <  1:1799710/100‑1 (MQ=255)
                                                                          gCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAAt                     >  2:3442389/1‑100 (MQ=255)
                                                                           cGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATg                    <  1:1347823/100‑1 (MQ=255)
                                                                            ggAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATgg                   >  2:488305/1‑100 (MQ=255)
                                                                             gAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGt                  >  2:131380/1‑100 (MQ=255)
                                                                             gAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGt                  <  2:376460/100‑1 (MQ=255)
                                                                               aGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGttt                <  2:3325266/100‑1 (MQ=255)
                                                                               aGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGttt                <  2:1253853/100‑1 (MQ=255)
                                                                               aGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGttt                <  2:3436594/100‑1 (MQ=255)
                                                                               aGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGttt                <  2:3230730/100‑1 (MQ=255)
                                                                               aGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGttt                <  2:2366797/100‑1 (MQ=255)
                                                                                   agtaaagtaaCCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTc            >  1:486710/1‑100 (MQ=255)
                                                                                    gtaaagtaaCCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTcc           >  1:1817714/1‑100 (MQ=255)
                                                                                     taaagtaaCCGAGGCTGTTACAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCg          <  2:2086053/100‑1 (MQ=255)
                                                                                      aaagtaaCCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCgg         <  1:3346425/100‑1 (MQ=255)
                                                                                      aaagtaaCCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCgg         <  1:2304913/100‑1 (MQ=255)
                                                                                       aagtaaCCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCgg         >  1:1300546/1‑99 (MQ=255)
                                                                                       aagtaaCCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGa        <  1:217248/100‑1 (MQ=255)
                                                                                        agtaaCCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGaa       >  2:837107/1‑100 (MQ=255)
                                                                                           aaCCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACt    >  2:2231262/1‑100 (MQ=255)
                                                                                            aCCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACtt   >  1:2680616/1‑100 (MQ=255)
                                                                                            aCCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACtt   >  2:2667182/1‑100 (MQ=255)
                                                                                             ccGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACttt  <  2:771649/100‑1 (MQ=255)
                                                                                                |                                                                                                
CCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACTTT  >  NC_000913/2250114‑2250306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: