Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,279,021 G→A A247V (GCG→GTG)  bcr ← bicyclomycin/cysteine/sulfonamide efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,279,0210GA97.9% 446.9 / ‑0.7 140A247V (GCG→GTG) bcrbicyclomycin/cysteine/sulfonamide efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base A (82/55);  total (82/58)
Fisher's exact test for biased strand distribution p-value = 6.89e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

Only 100 of 146 total aligned reads displayed.

TTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCGCGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGA  >  NC_000913/2278923‑2279117
                                                                                                  |                                                                                                
ttAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGgcgc                                                                                                 >  2:1550015/1‑100 (MQ=255)
    cgcgCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCacgacg                                                                                             >  2:649559/1‑100 (MQ=255)
    cgcgCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCacgacg                                                                                             <  2:1739090/100‑1 (MQ=255)
     gcgcCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGt                                                                                            >  2:1164288/1‑100 (MQ=255)
      cgcCAATGCGGCGGACGAAGCGGCTGCTAAAGATGGTCATCACGAACAGCAAAACCATGTTTAGCGCAAAGTAATAACCAAAGATTTCCGGCacgacggg                                                                                           >  2:1165521/1‑98 (MQ=255)
        ccAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGt                                                                                         >  1:2742859/1‑100 (MQ=255)
           aTGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTaa                                                                                      <  2:902443/100‑1 (MQ=255)
            tGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAAt                                                                                     <  1:432806/100‑1 (MQ=255)
              cggcggACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAAttt                                                                                   <  1:1242136/100‑1 (MQ=255)
               ggcggACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCACATTTTTCCGGCACGACGTGGTTAATTTc                                                                                  >  2:129983/1‑100 (MQ=255)
               ggcggACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTc                                                                                  <  2:1114197/100‑1 (MQ=255)
                gcggACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCa                                                                                 <  2:258974/100‑1 (MQ=255)
                 aggACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCaa                                                                                >  1:852794/2‑100 (MQ=255)
                   gACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAata                                                                              <  1:1787613/100‑1 (MQ=255)
                       aaGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATaaa                                                                          >  1:1919580/1‑100 (MQ=255)
                       aaGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATaaa                                                                          >  1:2130985/1‑100 (MQ=255)
                        ttccatctttAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATaaac                                                                         <  1:2965572/92‑1 (MQ=255)
                        aGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATaaac                                                                         <  1:1052173/100‑1 (MQ=255)
                           ggCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATaaacaaa                                                                      >  2:2161176/1‑100 (MQ=255)
                           ggCTGTTAAAGATGGACATAACGAACAGAAAAACAATGGTTAGCGCAAAGTAATAACCAAAATTTTCCGGCACGACGTGGTTAATTTCAATATaaacaaa                                                                      <  1:1490352/100‑1 (MQ=255)
                            gCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATaaacaaac                                                                     >  2:2907042/1‑100 (MQ=255)
                             cTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACg                                                                    >  2:2786184/1‑100 (MQ=255)
                             cTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACg                                                                    >  2:1833386/1‑100 (MQ=255)
                             cTGTTAAAGATGGTCATCACGAACAGAAAAACAATGATTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACg                                                                    >  2:2452003/1‑100 (MQ=255)
                              tGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACgg                                                                   <  2:2043775/100‑1 (MQ=255)
                              tGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACgg                                                                   >  2:2160190/1‑100 (MQ=255)
                               gTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGt                                                                  >  2:3166335/1‑100 (MQ=255)
                                 tAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTcc                                                                <  1:412774/100‑1 (MQ=255)
                                 tAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTcc                                                                >  2:2462226/1‑100 (MQ=255)
                                   aaGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCgg                                                              >  2:1058168/1‑100 (MQ=255)
                                   aaGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCgg                                                              >  2:3202771/1‑100 (MQ=255)
                                    aGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGgc                                                             <  2:1998883/100‑1 (MQ=255)
                                    aGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGgc                                                             >  2:3082571/1‑100 (MQ=255)
                                    aGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGgc                                                             <  1:2525705/100‑1 (MQ=255)
                                     gATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGgcg                                                            >  1:2578434/1‑100 (MQ=255)
                                     gATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGgcg                                                            >  2:2995896/1‑100 (MQ=255)
                                      aTGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGgcgc                                                           <  1:1907984/100‑1 (MQ=255)
                                        ggTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCtt                                                         <  1:1348795/100‑1 (MQ=255)
                                         gTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTa                                                        >  1:363452/1‑100 (MQ=255)
                                         gTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTa                                                        >  2:1056418/1‑100 (MQ=255)
                                             tcaCGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGaa                                                    >  1:3226792/1‑100 (MQ=255)
                                              caCGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAAt                                                   >  2:1443267/1‑100 (MQ=255)
                                              caCGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTACGGCACGACGTGGTTAATTTAAATATAAACAAACGGTCCGGCGCTTAAGAAt                                                   >  1:484918/1‑100 (MQ=255)
                                               aCGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATg                                                  <  1:2368931/100‑1 (MQ=255)
                                               aCGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATg                                                  <  2:3103348/100‑1 (MQ=255)
                                               aCGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATg                                                  >  2:477134/1‑100 (MQ=255)
                                                cGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATga                                                 >  1:3404197/1‑100 (MQ=255)
                                                  aaCAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATgaga                                               <  1:1191123/100‑1 (MQ=255)
                                                  aaCAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATgaga                                               <  1:1074246/100‑1 (MQ=255)
                                                   aCAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCGCGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGaa                                              <  2:941141/100‑1 (MQ=255)
                                                   aCAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGaa                                              <  2:1959047/100‑1 (MQ=255)
                                                    cAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAAc                                             >  1:843003/1‑100 (MQ=255)
                                                    cAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAAc                                             <  1:1976268/100‑1 (MQ=255)
                                                    cAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAAc                                             >  2:1730983/1‑100 (MQ=255)
                                                     aGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACa                                            >  1:1417517/1‑100 (MQ=255)
                                                      gAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACAt                                           >  1:2729811/1‑100 (MQ=255)
                                                         aaaCAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATccc                                        <  2:1477615/100‑1 (MQ=255)
                                                         aaaCAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATccc                                        <  2:1480825/100‑1 (MQ=255)
                                                           aCAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCgg                                      >  2:1121022/1‑100 (MQ=255)
                                                           aCAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCgg                                      <  1:649551/100‑1 (MQ=255)
                                                           aCAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCgg                                      <  2:2729854/100‑1 (MQ=255)
                                                           aCAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCgg                                      >  2:3458956/1‑100 (MQ=255)
                                                            cAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGc                                     <  2:1927193/100‑1 (MQ=255)
                                                            cAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGc                                     <  2:3225910/100‑1 (MQ=255)
                                                            cAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGc                                     <  1:1359648/100‑1 (MQ=255)
                                                            cAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGc                                     >  2:343835/1‑100 (MQ=255)
                                                            cAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCAGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCGTAAGAATGAGAACAACCCgg                                      >  1:2896146/1‑99 (MQ=255)
                                                                   tAGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTg                              <  1:2516869/100‑1 (MQ=255)
                                                                    aGCGCAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGa                             <  1:2786140/100‑1 (MQ=255)
                                                                     gcgcAAAGTAATAACCAAAGTTTTCCGGCGCGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGaa                            <  2:860792/100‑1 (MQ=255)
                                                                     gcgcAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGaa                            <  1:196859/100‑1 (MQ=255)
                                                                     gcgcAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGaa                            >  2:2965392/1‑100 (MQ=255)
                                                                        cAAAGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCACGGCAAAGCTGAAAcc                         >  1:2922700/1‑100 (MQ=255)
                                                                          aaGTAATAACCAAATTTTTCAGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAAcc                         <  1:3434251/98‑1 (MQ=255)
                                                                          aaGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCAc                       >  1:1615150/1‑100 (MQ=255)
                                                                           aGTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACt                      <  2:3365128/100‑1 (MQ=255)
                                                                            tccgatctcAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTg                     <  2:428236/92‑1 (MQ=255)
                                                                            gTAATAACCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATACCGGCAAAGCTGAAACCACTg                     >  1:3103502/1‑100 (MQ=255)
                                                                             taataaCCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTgg                    <  2:1748452/100‑1 (MQ=255)
                                                                              aataaCCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGc                   >  1:169036/1‑100 (MQ=255)
                                                                               ataaCCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCa                  <  2:446157/100‑1 (MQ=255)
                                                                                 aaCCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAg                <  1:2463469/100‑1 (MQ=255)
                                                                                  aCCAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGc               <  2:501660/100‑1 (MQ=255)
                                                                                   ccAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCa              <  1:3309816/100‑1 (MQ=255)
                                                                                   ccAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCa              >  2:1536561/1‑100 (MQ=255)
                                                                                   ccAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCa              >  2:661642/1‑100 (MQ=255)
                                                                                   ccAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCa              >  2:37615/1‑100 (MQ=255)
                                                                                    cAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCAt             >  2:1823234/1‑100 (MQ=255)
                                                                                    cAAAGTTTTCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGACAAGCa              >  1:3249492/1‑99 (MQ=255)
                                                                                         ttttCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGc        <  1:1056404/100‑1 (MQ=255)
                                                                                         ttttCCGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGc        <  2:1117720/100‑1 (MQ=255)
                                                                                             ccGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAg    >  1:2046160/1‑100 (MQ=255)
                                                                                             ccGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAg    >  1:2636078/1‑100 (MQ=255)
                                                                                             ccGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAg    >  1:3013354/1‑100 (MQ=255)
                                                                                             ccGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAg    <  1:60480/100‑1 (MQ=255)
                                                                                              cGGCACGGCGTGGTTAATTGCAATATAAATAAACGGTCCGGCGCTTAAGAATGAGAACATACCGGCAAAGCTGGAACCACTGCCAAGCATGTAGCTCacg   >  1:2954042/1‑98 (MQ=255)
                                                                                              cGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAgg   >  2:858489/1‑100 (MQ=255)
                                                                                              cGGCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAgg   >  1:680701/1‑100 (MQ=255)
                                                                                               ggCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGa  >  2:3281520/1‑100 (MQ=255)
                                                                                               ggCACGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGa  >  1:56932/1‑100 (MQ=255)
                                                                                                  |                                                                                                
TTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATCACGAACAGAAAAACAATGTTTAGCGCAAAGTAATAACCAAAGTTTTCCGGCGCGACGTGGTTAATTTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGA  >  NC_000913/2278923‑2279117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: