Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,420,727 G→A G36D (GGC→GAC)  yfcG → GSH‑dependent disulfide bond oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,420,7270GA99.3% 468.0 / ‑4.8 145G36D (GGC→GAC) yfcGGSH‑dependent disulfide bond oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (86/57);  total (87/58)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

Only 100 of 145 total aligned reads displayed.

CTCTATTTCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAACCGCTAAG  >  NC_000913/2420630‑2420823
                                                                                                 |                                                                                                
ctctATTTCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACg                                                                                                <  2:2497322/100‑1 (MQ=255)
 tctATTTCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACgg                                                                                               >  1:724176/1‑100 (MQ=255)
  ctATTTCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGt                                                                                              >  1:2636453/1‑100 (MQ=255)
  ctATTTCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGt                                                                                              <  2:1680225/100‑1 (MQ=255)
  ctATTTCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCGTGATTAAGGTAGACCTGGGGAAAGACGGt                                                                                              <  1:1319311/100‑1 (MQ=255)
  ctATTTCGCCCCGACACACAATGGCCACAAAATTACGATGTTTCTCGAAGAAGCAGAGATGGATTATAGCTGGATTAAGGTAGACCTGGGGAAAGCCgg                                                                                               >  1:1052157/1‑99 (MQ=255)
   tATTTCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTc                                                                                             <  2:3476561/100‑1 (MQ=255)
    aTTTCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCa                                                                                            <  1:1755858/100‑1 (MQ=255)
     tttCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAg                                                                                           >  1:1928326/1‑100 (MQ=255)
      ttCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGt                                                                                          >  2:2528113/1‑100 (MQ=255)
      ttCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGt                                                                                          >  2:82107/1‑100 (MQ=255)
        cGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGttt                                                                                        <  1:401440/100‑1 (MQ=255)
          ccccGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCg                                                                                      >  2:2716039/1‑100 (MQ=255)
             cGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTcc                                                                                   <  1:1220718/100‑1 (MQ=255)
             cGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTcc                                                                                   <  1:614755/100‑1 (MQ=255)
              gACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCg                                                                                  <  2:1225558/100‑1 (MQ=255)
              gACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCg                                                                                  <  1:2560549/100‑1 (MQ=255)
                  cccAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAAt                                                                              >  2:2972315/1‑100 (MQ=255)
                    cAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAAttt                                                                            >  2:2458954/1‑100 (MQ=255)
                      aTGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGGTCTGTATTATCGCTTTATTAAGGTATTCCTGGGGAAAGACGGTCAGTTTCGTCCGGAAttttt                                                                          <  1:2967356/100‑1 (MQ=255)
                       tGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTg                                                                         <  1:3406893/100‑1 (MQ=255)
                         gTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTgcg                                                                       >  1:1553909/1‑100 (MQ=255)
                          tCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTgcgc                                                                      >  2:1081905/1‑100 (MQ=255)
                           cacaAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCa                                                                     >  2:1112050/1‑100 (MQ=255)
                           cacaAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGCCAGTTTCGTCCGGAATTTTTGCGCa                                                                     <  1:2662092/100‑1 (MQ=255)
                            acaAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCAt                                                                    >  1:3142050/1‑100 (MQ=255)
                             caAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCAtt                                                                   >  2:2432228/1‑100 (MQ=255)
                             caAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCAtt                                                                   >  2:3464245/1‑100 (MQ=255)
                             caAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCAtt                                                                   >  2:2396969/1‑100 (MQ=255)
                             caAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCAtt                                                                   >  2:1486486/1‑100 (MQ=255)
                             caAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCAtt                                                                   >  2:718153/1‑100 (MQ=255)
                              aaaaTTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCAttt                                                                  >  2:1113972/1‑100 (MQ=255)
                                 aTTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGc                                                               <  1:1686753/100‑1 (MQ=255)
                                  ttACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGcc                                                              <  1:2653800/100‑1 (MQ=255)
                                   tACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGccc                                                             <  2:1158223/100‑1 (MQ=255)
                                    aCGCTGTTTCTCGAAGAAGCAGAGCAGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCca                                                            >  2:1604160/1‑100 (MQ=255)
                                     cGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCca                                                            <  2:474038/99‑1 (MQ=255)
                                      gCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCcaac                                                          <  2:257403/100‑1 (MQ=255)
                                       cTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCcaaca                                                         >  1:3256973/1‑100 (MQ=255)
                                       cTGTTTCTAGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCcaaca                                                         <  1:99005/100‑1 (MQ=255)
                                          tttCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCcaacaaca                                                      >  1:1708657/1‑100 (MQ=255)
                                           ttCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCcaacaaca                                                      >  1:748515/1‑99 (MQ=255)
                                            tctcGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACaaa                                                    >  1:486344/1‑100 (MQ=255)
                                            tctcGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACaaa                                                    >  2:557607/1‑100 (MQ=255)
                                             ctcGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACaaaa                                                   >  2:1393967/1‑100 (MQ=255)
                                                gaagaaGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTc                                                >  1:598315/1‑100 (MQ=255)
                                                 aagaagCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTcc                                               >  2:1266995/1‑100 (MQ=255)
                                                  agaagCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCg                                              <  2:1536945/100‑1 (MQ=255)
                                                   gaagCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCgg                                             <  2:1323849/100‑1 (MQ=255)
                                                     agCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCa                                           >  2:468726/1‑100 (MQ=255)
                                                       cAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAAt                                         >  1:84338/1‑100 (MQ=255)
                                                        agagCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAAtt                                        >  2:3030377/1‑100 (MQ=255)
                                                         gagCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCCACAACCAAATTACGGCACtt                                        >  2:2882814/1‑99 (MQ=255)
                                                           gCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAAttgtt                                     <  1:630725/100‑1 (MQ=255)
                                                           gCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAAttgtt                                     >  1:1653930/1‑100 (MQ=255)
                                                           gCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAAttgtt                                     <  2:2636491/100‑1 (MQ=255)
                                                            cTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAAttgttg                                    <  1:3401774/100‑1 (MQ=255)
                                                            cTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAAttgttg                                    <  2:532212/100‑1 (MQ=255)
                                                            cTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAAttgttg                                    <  2:452710/100‑1 (MQ=255)
                                                              ggATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGAt                                  <  1:2993984/100‑1 (MQ=255)
                                                              ggATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGAt                                  >  2:2226367/1‑100 (MQ=255)
                                                                aTTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCa                                >  1:3306469/1‑100 (MQ=255)
                                                                  tATCGCTTGATTAAGGTATACCTAGGGAAAGACTGTCAGTTTCGTCCGGTAGTTTTTCGCATTTCGCCCATCACCAAAATTCCGGCAATTGTTGATCAt                               <  1:3302064/99‑1 (MQ=255)
                                                                  tATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCAtt                              >  2:973559/1‑100 (MQ=255)
                                                                   agcGCTCGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATtc                             <  2:3078121/98‑1 (MQ=255)
                                                                   aTCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATtc                             >  1:2730717/1‑100 (MQ=255)
                                                                   aTCGCTTGATTAAGGTAGACCTGGGGAAAGACGCTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATtc                             <  1:3275394/100‑1 (MQ=255)
                                                                      gCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTcc                          >  2:2667067/1‑100 (MQ=255)
                                                                        ttGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTg                        >  2:342677/1‑100 (MQ=255)
                                                                        ttGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTg                        <  2:3143210/100‑1 (MQ=255)
                                                                         tGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGc                       >  2:210382/1‑100 (MQ=255)
                                                                           aTTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCg                     >  1:1891348/1‑100 (MQ=255)
                                                                           aTTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAAGTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGcc                      >  1:3146519/1‑99 (MQ=255)
                                                                            ttAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGa                    >  2:1825955/1‑100 (MQ=255)
                                                                            ttAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAAGTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGa                    >  1:2536772/1‑100 (MQ=255)
                                                                             tAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGAt                   >  1:2172398/1‑100 (MQ=255)
                                                                              aaGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATg                  >  2:651822/1‑100 (MQ=255)
                                                                              aaGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATg                  <  1:1147255/100‑1 (MQ=255)
                                                                              aaGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATg                  <  2:1362764/100‑1 (MQ=255)
                                                                              aaGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATg                  >  2:175272/1‑100 (MQ=255)
                                                                               aGGTAGAGCTGGGGAAAGACGGTCAGTGTCGTCGGGAATTTTTGCGCATTTCGCGCGATATCAACATTCCGGCAATTGTTGATCATTTTCCTGCCGGTgg                 >  1:1071021/1‑100 (MQ=255)
                                                                               aGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATgc                 >  2:3120158/1‑99 (MQ=255)
                                                                                ggTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATggc                >  2:2937781/1‑100 (MQ=255)
                                                                                  tAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATggcgg              <  1:825947/100‑1 (MQ=255)
                                                                                   aGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATggcggc             <  2:1141965/100‑1 (MQ=255)
                                                                                   aGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATggcggc             <  1:1696261/100‑1 (MQ=255)
                                                                                    gACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCgccatcggca            <  2:2969154/100‑10 (MQ=255)
                                                                                      ccTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGaa          >  1:1001914/1‑100 (MQ=255)
                                                                                      ccTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGaa          >  1:1835807/1‑100 (MQ=255)
                                                                                      ccTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGaa          >  1:2790502/1‑100 (MQ=255)
                                                                                       cTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAAc         >  2:2906570/1‑100 (MQ=255)
                                                                                        tGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAAcc        <  1:1280162/100‑1 (MQ=255)
                                                                                        nggggAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAAcc        >  1:223244/2‑100 (MQ=255)
                                                                                         ggggAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGTCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAACCg       <  2:3334813/100‑1 (MQ=255)
                                                                                         ggggAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAACCg       >  1:2716817/1‑100 (MQ=255)
                                                                                         ggggAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAACCg       >  2:1976485/1‑100 (MQ=255)
                                                                                         ggggAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAACCg       >  1:2699868/1‑100 (MQ=255)
                                                                                         ggggAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAACCg       <  1:3195587/100‑1 (MQ=255)
                                                                                           ggAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGTCGATGGCGGCGAACCGCt     >  1:983476/1‑100 (MQ=255)
                                                                                           ggAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAACCGCt     >  2:226050/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CTCTATTTCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAACCGCTAAG  >  NC_000913/2420630‑2420823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑