Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,493,645 G→A intergenic (‑391/+122) frc ← / ← yfdX formyl‑CoA transferase, NAD(P)‑binding/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,493,6450GA97.6% 400.9 / ‑0.5 124intergenic (‑391/+122)frc/yfdXformyl‑CoA transferase, NAD(P)‑binding/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (64/57);  total (66/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-01

Only 100 of 125 total aligned reads displayed.

ATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTAC  >  NC_000913/2493549‑2493744
                                                                                                |                                                                                                   
aTGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGa                                                                                                  <  2:770834/100‑1 (MQ=255)
 tGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAg                                                                                                 <  2:1288392/100‑1 (MQ=255)
  gATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGt                                                                                                >  1:2051047/1‑100 (MQ=255)
  gATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGt                                                                                                >  2:2882281/1‑100 (MQ=255)
   aTTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGtt                                                                                               >  1:1371354/1‑100 (MQ=255)
   aTTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTGGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAg                                                                                                 <  1:2211718/98‑1 (MQ=255)
       cgctttaaaaaGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTtata                                                                                           >  2:1492590/9‑100 (MQ=255)
       cGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTtata                                                                                           <  1:73832/100‑1 (MQ=255)
       cGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTtata                                                                                           <  1:137741/100‑1 (MQ=255)
        gCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAg                                                                                          <  2:1491939/100‑1 (MQ=255)
        gCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAg                                                                                          <  1:1694642/100‑1 (MQ=255)
        gCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAg                                                                                          >  2:1569433/1‑100 (MQ=255)
         cTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGc                                                                                         >  1:1299900/1‑100 (MQ=255)
         cTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGc                                                                                         >  2:1488821/1‑100 (MQ=255)
         cTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGc                                                                                         >  2:186798/1‑100 (MQ=255)
             ttAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACcg                                                                                     <  2:2876414/100‑1 (MQ=255)
             ttAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACcg                                                                                     <  2:302005/100‑1 (MQ=255)
              tAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACcgc                                                                                    <  1:3267049/100‑1 (MQ=255)
              tAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACcgc                                                                                    >  2:1060665/1‑100 (MQ=255)
              tAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACcgc                                                                                    >  1:2635652/1‑100 (MQ=255)
                 aGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAg                                                                                 >  1:1595751/1‑100 (MQ=255)
                 aGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAg                                                                                 <  1:2188383/100‑1 (MQ=255)
                 aGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAg                                                                                 >  1:2338308/1‑100 (MQ=255)
                  gCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAgc                                                                                <  2:1817329/100‑1 (MQ=255)
                    gTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAgcgc                                                                              >  2:1287665/1‑100 (MQ=255)
                    gTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAgcgc                                                                              >  1:2665849/1‑100 (MQ=255)
                    gTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAgcg                                                                               <  1:3245223/99‑1 (MQ=255)
                     tACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGcc                                                                             <  2:1207490/100‑1 (MQ=255)
                       ccATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCaa                                                                           >  1:1323812/1‑100 (MQ=255)
                        naTAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCaaa                                                                          >  1:2354978/2‑100 (MQ=255)
                        cATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCaaa                                                                          >  1:1071243/1‑100 (MQ=255)
                         aTAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAaat                                                                         >  2:990415/1‑100 (MQ=255)
                         aTAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAaat                                                                         <  2:30674/100‑1 (MQ=255)
                           aaaaCATAACATATTAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAaataa                                                                       >  2:2347009/1‑100 (MQ=255)
                             aacataacatAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAAtt                                                                     >  2:71777/1‑100 (MQ=255)
                             aacataacatAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAAtt                                                                     <  2:747759/100‑1 (MQ=255)
                               cataacataaTAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAAtttt                                                                   <  2:2299484/100‑1 (MQ=255)
                               cataacataaTAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGGTTATAGCACCGCGAGCGCCAAATAAtttt                                                                   <  1:1438914/100‑1 (MQ=255)
                                 taacataaTAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTAt                                                                 <  1:1486182/100‑1 (MQ=255)
                                 taacataaTAAATGATTTGTAATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTAt                                                                 >  1:2118324/1‑100 (MQ=255)
                                  aacataaTAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCTAAATAATTTTATg                                                                >  2:3145681/1‑100 (MQ=255)
                                  aacataaTAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATg                                                                >  1:2496358/1‑100 (MQ=255)
                                  aacataaTAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCAAAATAATTTTATg                                                                >  1:2869693/1‑100 (MQ=255)
                                   acataaTAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGa                                                               >  1:1110498/1‑100 (MQ=255)
                                   acataaTAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGa                                                               >  2:1896706/1‑100 (MQ=255)
                                    cataaTAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCATCGCGAGCGCCAAATAATTTTATGAc                                                              >  1:1013332/1‑100 (MQ=255)
                                        ataaATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACcaca                                                          >  2:3399081/1‑100 (MQ=255)
                                           aaTGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAAcc                                                       <  2:2938217/100‑1 (MQ=255)
                                           aaTGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAAcc                                                       <  1:1434719/100‑1 (MQ=255)
                                            aTGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCt                                                      >  2:3233099/1‑100 (MQ=255)
                                                     gatgaCACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATtg                                             <  2:3146933/100‑1 (MQ=255)
                                                     gatgaCACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATtg                                             <  1:5216/100‑1 (MQ=255)
                                                       tgaCACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATtgtg                                           <  1:2539371/100‑1 (MQ=255)
                                                        gaCACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTgg                                          <  2:1464582/100‑1 (MQ=255)
                                                        gaCACGGGATTATTGCGATTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTgg                                          <  2:30124/100‑1 (MQ=255)
                                                         acacGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGt                                         >  2:2597987/1‑100 (MQ=255)
                                                         acacGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGt                                         <  1:3157333/100‑1 (MQ=255)
                                                         acacGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGt                                         <  2:1030914/100‑1 (MQ=255)
                                                           acGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCa                                       <  1:2393240/100‑1 (MQ=255)
                                                           acGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCa                                       <  2:2039096/100‑1 (MQ=255)
                                                           acGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCa                                       <  2:3370832/100‑1 (MQ=255)
                                                            cGGGATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCAt                                      >  2:2264949/1‑100 (MQ=255)
                                                             gggATTATTGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATa                                     <  2:1962358/100‑1 (MQ=255)
                                                                attattGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATaaaa                                  <  2:35124/100‑1 (MQ=255)
                                                                   attGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGCCCACAACCTGTCGTATTGTGGTCATAAAAAAt                               <  2:3295844/100‑1 (MQ=255)
                                                                   attGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAAt                               >  1:3029759/1‑100 (MQ=255)
                                                                    ttGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAAtt                              <  2:2243213/100‑1 (MQ=255)
                                                                     tGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTg                             >  1:2374619/1‑100 (MQ=255)
                                                                     tGCGGTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTg                             <  1:2588651/100‑1 (MQ=255)
                                                                     tGCGGTTTACTTTGAGAAATATAAAATACGAGTTTATAGCACCGCAAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTCGTCATAAAAAAt                               <  2:2848436/98‑1 (MQ=255)
                                                                        ggTTTACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGGGGTCATAAAAAATTGTCg                          >  1:388184/1‑100 (MQ=255)
                                                                            tACTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTg                      >  2:1075863/1‑100 (MQ=255)
                                                                             aCTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTc                           >  1:2164634/1‑94 (MQ=255)
                                                                             aCTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTgg                     >  1:965342/1‑100 (MQ=255)
                                                                              cTTTGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTgga                    >  2:2477169/1‑100 (MQ=255)
                                                                               tttGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTggag                   <  2:1110512/100‑1 (MQ=255)
                                                                                ttGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTggagg                  <  2:1941305/100‑1 (MQ=255)
                                                                                ttGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTggagg                  >  2:3424586/1‑100 (MQ=255)
                                                                                ttGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTggagg                  >  1:50278/1‑100 (MQ=255)
                                                                                ttGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTggagg                  <  2:2307464/100‑1 (MQ=255)
                                                                                 tGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTggaggg                 <  2:2617396/100‑1 (MQ=255)
                                                                                 tGAGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTggaggg                 >  2:3312471/1‑100 (MQ=255)
                                                                                   aGGAATATAAAATACGGGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTggagggag               <  1:1164266/100‑1 (MQ=255)
                                                                                   aGGAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTggagggag               <  2:3181049/100‑1 (MQ=255)
                                                                                    ggAATATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGt              <  2:176800/100‑1 (MQ=255)
                                                                                      aaTATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTa            <  1:1697238/100‑1 (MQ=255)
                                                                                      aaTATAAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTa            >  2:271312/1‑100 (MQ=255)
                                                                                        tataAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTaaa          <  1:3471514/100‑1 (MQ=255)
                                                                                         ataAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTa            <  1:730916/97‑1 (MQ=255)
                                                                                          taAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATc        >  2:409887/1‑100 (MQ=255)
                                                                                          taAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATc        >  2:63671/1‑100 (MQ=255)
                                                                                          taAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATc        >  1:188389/1‑100 (MQ=255)
                                                                                          taAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATc        >  1:2916631/1‑100 (MQ=255)
                                                                                          taAAATACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATc        >  2:85747/1‑100 (MQ=255)
                                                                                           aaaaTACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATcc       <  2:42370/100‑1 (MQ=255)
                                                                                           aaaaTACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATcc       >  2:895812/1‑100 (MQ=255)
                                                                                           aaaaTACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATcc       <  2:952565/100‑1 (MQ=255)
                                                                                            aaaTACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCt      >  2:2061688/1‑100 (MQ=255)
                                                                                            aaaTACGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCt      <  1:472182/100‑1 (MQ=255)
                                                                                                gCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTAc  <  1:71776/100‑1 (MQ=255)
                                                                                                |                                                                                                   
ATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTAC  >  NC_000913/2493549‑2493744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: