Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,521,967 C→A A171S (GCG→TCG)  xapR ← transcriptional activator of xapAB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,521,9670CA99.2% 446.9 / ‑6.3 134A171S (GCG→TCG) xapRtranscriptional activator of xapAB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (68/65);  minor base G (0/1);  total (68/66)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 137 total aligned reads displayed.

GGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCA  >  NC_000913/2521870‑2522062
                                                                                                 |                                                                                               
ggcggcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAacgacg                                                                                               <  2:3226120/100‑1 (MQ=255)
 gcggcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAAcgacga                                                                                              >  2:1502596/1‑100 (MQ=255)
  cggcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGAt                                                                                             >  2:2723586/1‑100 (MQ=255)
    gcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTc                                                                                           >  1:3186342/1‑100 (MQ=255)
    gcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTc                                                                                           >  2:3000349/1‑100 (MQ=255)
      aTTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCAt                                                                                         >  1:3123995/1‑100 (MQ=255)
      aTTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCAt                                                                                         <  1:2657608/100‑1 (MQ=255)
      aTTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCAt                                                                                         <  1:34854/100‑1 (MQ=255)
       ttGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATg                                                                                        <  2:620252/100‑1 (MQ=255)
       ttGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATg                                                                                        <  1:3177873/100‑1 (MQ=255)
        tGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGc                                                                                       >  2:1061630/1‑100 (MQ=255)
            aCAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAAcc                                                                                   <  1:19532/100‑1 (MQ=255)
             cAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCg                                                                                  <  1:1107631/100‑1 (MQ=255)
             cAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCg                                                                                  >  2:1253868/1‑100 (MQ=255)
             cAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCg                                                                                  >  2:2919013/1‑100 (MQ=255)
                aaTACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTaa                                                                               <  2:2158716/100‑1 (MQ=255)
                aaTACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTaa                                                                               <  2:3107703/100‑1 (MQ=255)
                 aTACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAg                                                                              >  1:1323141/1‑100 (MQ=255)
                  tACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGc                                                                             >  2:2310615/1‑100 (MQ=255)
                     tCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTgg                                                                          <  1:2915026/100‑1 (MQ=255)
                      cGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGt                                                                         >  2:1207435/1‑100 (MQ=255)
                      cGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGt                                                                         >  2:367396/1‑100 (MQ=255)
                      cGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGt                                                                         <  1:2611146/100‑1 (MQ=255)
                      cGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGt                                                                         >  1:1916790/1‑100 (MQ=255)
                        tCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTan                                                                       <  1:1640840/100‑2 (MQ=255)
                        tCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTaa                                                                       >  1:2336543/1‑100 (MQ=255)
                         cACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTaaa                                                                      >  1:2169838/1‑100 (MQ=255)
                         cACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTaaa                                                                      >  1:2640486/1‑100 (MQ=255)
                          aCGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTaaaa                                                                     <  2:2218529/100‑1 (MQ=255)
                           cGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAAc                                                                    >  1:2794699/1‑100 (MQ=255)
                            gTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAAcc                                                                   <  1:1909815/100‑1 (MQ=255)
                              aGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAg                                                                 >  2:2908191/1‑100 (MQ=255)
                              aGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAg                                                                 <  2:779795/100‑1 (MQ=255)
                               gcgcTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGt                                                                >  1:3262965/1‑100 (MQ=255)
                                 gcTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCg                                                              >  1:2059788/1‑100 (MQ=255)
                                 gcTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCg                                                              >  2:440331/1‑100 (MQ=255)
                                 gcTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCg                                                              >  2:1953715/1‑100 (MQ=255)
                                   ttCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggt                                                            <  1:1207420/100‑1 (MQ=255)
                                    tCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGATACGACGATTCTTGCAACCGTAAGCTGGTTAAACCAGTCggag                                                           <  2:2343212/100‑3 (MQ=255)
                                     ccAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggtgg                                                          >  1:782735/1‑100 (MQ=255)
                                     ccAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGAAAGCTGGTAAAACCAGTCggtgg                                                          <  1:997597/100‑1 (MQ=255)
                                      cAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggtggt                                                         >  2:1542758/1‑100 (MQ=255)
                                      cAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggtggt                                                         <  2:1995588/100‑1 (MQ=255)
                                       aGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGtt                                                        >  2:2684671/1‑100 (MQ=255)
                                          gggACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTg                                                     <  2:1165376/100‑1 (MQ=255)
                                            gACGATGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTc                                                   <  1:388934/100‑1 (MQ=255)
                                             aCGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCg                                                  <  1:1270621/100‑1 (MQ=255)
                                              cGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGc                                                 <  2:2824417/100‑1 (MQ=255)
                                                gTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCa                                               >  1:1466164/1‑100 (MQ=255)
                                                gTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCa                                               <  2:3078095/100‑1 (MQ=255)
                                                 tGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCAt                                              >  2:2337892/1‑100 (MQ=255)
                                                  ggAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCAtt                                             >  1:2919073/1‑100 (MQ=255)
                                                  ggAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCAtt                                             >  1:971140/1‑100 (MQ=255)
                                                   gAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTc                                            >  2:3309690/1‑100 (MQ=255)
                                                   gAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTc                                            >  1:2730346/1‑100 (MQ=255)
                                                    aaaaTGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCg                                           >  2:177555/1‑100 (MQ=255)
                                                      aaTGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGcc                                         >  1:1224147/1‑100 (MQ=255)
                                                       atgatgAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCa                                        <  1:1002838/100‑1 (MQ=255)
                                                          atgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGAt                                     <  1:3388902/100‑1 (MQ=255)
                                                            gagagaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAGAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATcc                                   >  1:2307474/1‑100 (MQ=255)
                                                            gagagaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATcc                                   >  1:1250253/1‑100 (MQ=255)
                                                             agagaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATccc                                  >  1:1544327/1‑100 (MQ=255)
                                                             agagaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATccc                                  >  2:2519397/1‑100 (MQ=255)
                                                             agagaTGATCCTCTTCAGCCATCGCCACCAGAACCGACGATTCATGCAACCGTAAGCCGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGAccc                                   <  1:1700769/99‑1 (MQ=255)
                                                              gagaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCg                                 <  1:651582/100‑1 (MQ=255)
                                                               agaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCgg                                <  1:2256535/100‑1 (MQ=255)
                                                               agaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCgg                                >  2:76816/1‑100 (MQ=255)
                                                                gatgatGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGc                               <  1:1006490/100‑1 (MQ=255)
                                                                 atgatgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCa                              <  2:1323160/100‑1 (MQ=255)
                                                                   gatgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATc                            >  1:3149220/1‑100 (MQ=255)
                                                                   gatgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATc                            >  2:2788397/1‑100 (MQ=255)
                                                                   gatgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATc                            >  2:67928/1‑100 (MQ=255)
                                                                   gatgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATc                            >  1:494362/1‑100 (MQ=255)
                                                                   gatgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATGCGCCAGATCCCGGCATc                            <  1:882403/100‑1 (MQ=255)
                                                                    atgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCa                           <  2:2660267/100‑1 (MQ=255)
                                                                     tgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCaa                          >  2:580178/1‑100 (MQ=255)
                                                                       ctctTCAGGCATCGCCACCAGAAACGACTATTCATGCAACCGTAAGCTGGTAAAACCAGGCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAg                         <  1:1042173/99‑1 (MQ=255)
                                                                        tctTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGtt                       >  2:431249/1‑100 (MQ=255)
                                                                         ctTCAGGCATCGCCACCAGAAACGACGATTCATGCACCCGTAAGCTGGCAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCACGTTc                      >  1:1418137/1‑100 (MQ=255)
                                                                         ctTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTc                      >  2:664260/1‑100 (MQ=255)
                                                                         ctTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTc                      <  1:440326/100‑1 (MQ=255)
                                                                           tCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTcgc                    <  2:604873/100‑1 (MQ=255)
                                                                           tCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCCCCATTCGCCAGATCCCGGCATCAAGTTcgc                    >  1:3179903/1‑100 (MQ=255)
                                                                            cAGGCATCGCCACCAGAAACGATGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTcgcg                   >  2:1726821/1‑100 (MQ=255)
                                                                            cAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTcgcg                   <  2:662677/100‑1 (MQ=255)
                                                                             aGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcgg                  <  1:1716805/100‑1 (MQ=255)
                                                                             aGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcgg                  <  2:3396938/100‑1 (MQ=255)
                                                                             aGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcgg                  <  2:3241452/100‑1 (MQ=255)
                                                                             aGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcgg                  <  2:1544349/100‑1 (MQ=255)
                                                                              ggCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcggc                 >  2:2801967/1‑100 (MQ=255)
                                                                               gCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcggcg                <  2:1345977/100‑1 (MQ=255)
                                                                                cATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGt               >  1:665322/1‑100 (MQ=255)
                                                                                cATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGt               >  1:239371/1‑100 (MQ=255)
                                                                                      caccaGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGc         <  2:2135509/100‑1 (MQ=255)
                                                                                        ccaGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCa        >  2:2745731/1‑99 (MQ=255)
                                                                                          aGAATCGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAgg     <  2:1669626/100‑1 (MQ=255)
                                                                                           gAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGc    <  1:2684629/100‑1 (MQ=255)
                                                                                            aaaCGACGATTCATGCAACCGTAAGCTGGTAAAATCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTCCCATCAAGGcc   <  1:1824908/100‑1 (MQ=255)
                                                                                            aaaCGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGcc   <  2:3336471/100‑1 (MQ=255)
                                                                                             aaCGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCa  >  2:1205741/1‑100 (MQ=255)
                                                                                                 |                                                                                               
GGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCA  >  NC_000913/2521870‑2522062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: