BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,530,925	T→C	Q103Q (CAA→CAG)	zipA ←	FtsZ stabilizer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,530,925	0	T	C	97.3%	494.8 / 0.4	148	Q103Q (CAA→CAG)	zipA	FtsZ stabilizer
Reads supporting (aligned to +/- strand): ref base T (3/1); new base C (63/80); total (66/82)
Fisher's exact test for biased strand distribution p-value = 3.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 148 total aligned reads displayed.

GTTCAGGCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGCATGATTC  >  NC_000913/2530828‑2531021

   cAGGCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGAcg                                                                                             >  2:840330/1‑100 (MQ=255)

     ggCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGTTGCTGGACCGGCTGAcgcg                                                                                           <  2:1655581/100‑1 (MQ=255)

     cgatctggCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGAcgcg                                                                                           <  1:1376937/94‑1 (MQ=255)

       cTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGc                                                                                         <  2:857739/100‑1 (MQ=255)

       cTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGc                                                                                         <  1:2428873/100‑1 (MQ=255)

       cTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGGACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGc                                                                                         >  2:1021962/1‑100 (MQ=255)

         gCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTg                                                                                       >  1:577054/1‑100 (MQ=255)

           ggCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGAcgcgagatcg                                                                                     >  2:1376957/1‑94 (MQ=255)

           ggCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTgcg                                                                                     <  2:2536572/100‑1 (MQ=255)

            gCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTgcgc                                                                                    <  1:1841412/100‑1 (MQ=255)

                  aggcaggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGc                                                                              <  2:303496/100‑1 (MQ=255)

                  aggcaggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGc                                                                              <  2:1134033/100‑1 (MQ=255)

                   ggcaggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCa                                                                             <  2:1784595/100‑1 (MQ=255)

                   ggcaggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCa                                                                             >  1:315057/1‑100 (MQ=255)

                    gcaggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCAt                                                                            <  1:777409/100‑1 (MQ=255)

                    gcaggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCAt                                                                            <  1:777134/100‑1 (MQ=255)

                     caggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATa                                                                           >  2:415742/1‑100 (MQ=255)

                     caggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATa                                                                           <  1:1261019/100‑1 (MQ=255)

                      aggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATaa                                                                          <  2:1196590/100‑1 (MQ=255)

                       ggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATa                                                                           >  1:2115735/1‑98 (MQ=255)

                         ctgctgCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGACGCATAAGGc                                                                       >  2:2845564/1‑100 (MQ=255)

                         ctgctgCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGc                                                                       <  1:3412351/100‑1 (MQ=255)

                          tgctgcACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCg                                                                      >  2:1502269/1‑100 (MQ=255)

                           gctgcACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCgg                                                                     >  2:481256/1‑100 (MQ=255)

                            ctgcACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCgg                                                                     >  2:3193057/1‑99 (MQ=255)

                            ctgcACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGt                                                                    <  1:2420758/100‑1 (MQ=255)

                            ctgcACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGt                                                                    <  1:3196331/100‑1 (MQ=255)

                            ctgcACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGCCTTATGCGTCTGCGCAGCCGcgtcagccggt                                                                    <  2:2442878/100‑11 (MQ=21)

                            cggcACCGGCAGCACTGGATGCGGCGTCCGTTGCGGAGGTACCTGCGCTCCAGGCCGCCGCCGGACGGGGTAACGCGGTTGCCCAGACGCATAAGGCGGt                                                                    <  1:829248/98‑1 (MQ=255)

                             tgcACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGtt                                                                   >  1:520092/1‑100 (MQ=255)

                              gcACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTg                                                                  <  1:2626845/100‑1 (MQ=255)

                               cACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTgg                                                                 <  1:1179980/100‑1 (MQ=255)

                                  cGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTAc                                                              <  1:2692581/100‑1 (MQ=255)

                                  cGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTAc                                                              <  1:944057/100‑1 (MQ=255)

                                   ggCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACt                                                             >  2:10985/1‑100 (MQ=255)

                                   ggCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACt                                                             <  1:1367905/100‑1 (MQ=255)

                                    gCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTg                                                            >  1:2261223/1‑100 (MQ=255)

                                     cTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTgg                                                           >  2:2033830/1‑100 (MQ=255)

                                      tGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGgt                                                          >  2:622484/1‑100 (MQ=255)

                                       gcgcTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGgtg                                                         <  1:1085140/100‑1 (MQ=255)

                                        cgcTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGgtgt                                                        <  1:2557137/100‑1 (MQ=255)

                                         gcTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGtt                                                       >  1:450321/1‑100 (MQ=255)

                                         gcTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGtt                                                       >  2:2313359/1‑100 (MQ=255)

                                          cTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTg                                                      >  1:430049/1‑100 (MQ=255)

                                          cTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTg                                                      >  2:3114127/1‑100 (MQ=255)

                                           tGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTgc                                                     <  2:709103/100‑1 (MQ=255)

                                            ggATGCGGTGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTgcg                                                    <  2:107418/100‑1 (MQ=255)

                                            ggATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTgcg                                                    >  2:1733117/1‑100 (MQ=255)

                                            ggATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTgcg                                                    <  1:176459/100‑1 (MQ=255)

                                            ggATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTgcg                                                    >  2:2506335/1‑100 (MQ=255)

                                               tGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCCGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTgcggcg                                                 <  2:2443817/100‑1 (MQ=255)

                                                  ggAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGTCGGTTGGTACTGGTGTTGCGGCgacg                                              <  2:2115766/100‑1 (MQ=255)

                                                  ggAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgacg                                              <  1:3042260/100‑1 (MQ=255)

                                                  ggAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgacg                                              <  1:3042335/100‑1 (MQ=255)

                                                  ggAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgacg                                              >  2:3312563/1‑100 (MQ=255)

                                                     gCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgacggac                                           <  2:2701763/100‑1 (MQ=255)

                                                     gCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgacggac                                           >  2:316830/1‑100 (MQ=255)

                                                      cATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgacggacg                                          <  1:2727859/100‑1 (MQ=255)

                                                      cATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgacggacg                                          <  1:3395122/100‑1 (MQ=255)

                                                        tGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGag                                        <  2:219019/100‑1 (MQ=255)

                                                             cggcggTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGcc                                   >  1:204098/1‑100 (MQ=255)

                                                             cggcggTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGcc                                   <  1:1332603/100‑1 (MQ=255)

                                                               gcggTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTc                                 <  1:1437155/100‑1 (MQ=255)

                                                                cggTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCa                                <  2:3389950/100‑1 (MQ=255)

                                                                cggTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCa                                <  1:2652497/100‑1 (MQ=255)

                                                                  gTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATg                              >  2:1164498/1‑100 (MQ=255)

                                                                     ccTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTc                           <  2:3370094/100‑1 (MQ=255)

                                                                      cTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTcc                          <  2:2803095/100‑1 (MQ=255)

                                                                         cgcTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGa                       >  2:3113654/1‑100 (MQ=255)

                                                                          gcTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGccccggct                      >  2:538045/1‑93 (MQ=255)

                                                                          gcTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGa                       <  1:273269/99‑1 (MQ=255)

                                                                           cTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGc                     <  1:1886599/100‑1 (MQ=255)

                                                                           cTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGc                     >  1:3289009/1‑100 (MQ=255)

                                                                           cTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGc                     >  1:2876290/1‑100 (MQ=255)

                                                                           cTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCAGAAGGCGGCTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGc                     >  1:542558/1‑100 (MQ=255)

                                                                              cAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGtt                  >  2:2959583/1‑100 (MQ=255)

                                                                              cAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACACATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGtt                  <  1:1326796/100‑1 (MQ=255)

                                                                               aGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTa                 <  2:3348105/100‑1 (MQ=255)

                                                                                ggcggcTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAg                >  2:1660902/1‑100 (MQ=255)

                                                                                ggcggcTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAg                >  2:1272001/1‑100 (MQ=255)

                                                                                ggcggcTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAg                <  2:2217479/100‑1 (MQ=255)

                                                                                  cggcTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGcc              <  2:2719331/100‑1 (MQ=255)

                                                                                  cggcTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGcc              >  1:1577360/1‑100 (MQ=255)

                                                                                  cggcTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGcc              <  2:3289060/100‑1 (MQ=255)

                                                                                   ggcTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCg             >  1:324638/1‑100 (MQ=255)

                                                                                    gctgctGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCgg            >  2:3249213/1‑100 (MQ=255)

                                                                                    gctgctGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCgg            <  2:1920057/100‑1 (MQ=255)

                                                                                     ctgctgGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCggg           >  1:462661/1‑100 (MQ=255)

                                                                                     ctgctgGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCggg           <  2:2370407/100‑1 (MQ=255)

                                                                                     ctgctgGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCggg           >  1:462874/1‑100 (MQ=255)

                                                                                     ctgctgGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCggg           >  1:1115488/1‑100 (MQ=255)

                                                                                       gctgGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGc         <  2:1514210/100‑1 (MQ=255)

                                                                                       gctgGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGc         >  2:1936007/1‑100 (MQ=255)

                                                                                       gctgGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTAGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGc         <  1:642072/100‑1 (MQ=255)

                                                                                       gctgGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGCTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGc         <  1:2217742/100‑1 (MQ=255)

                                                                                         tgGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGCAt       <  1:1670919/100‑1 (MQ=255)

                                                                                          ggACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGCATg      >  1:1104059/1‑100 (MQ=255)

                                                                                          ggACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGCATg      <  2:1411697/100‑1 (MQ=255)

                                                                                            aCCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGCATGAt    <  2:2689857/100‑1 (MQ=255)

                                                                                              cGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGCATGATTc  >  2:3420779/1‑100 (MQ=255)

GTTCAGGCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGCATGATTC  >  NC_000913/2530828‑2531021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`