Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,534,206 T→G R47R (CGT→CGG ptsI → PEP‑protein phosphotransferase of PTS system (enzyme I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,534,2060TG99.1% 387.3 / ‑4.3 114R47R (CGT→CGGptsIPEP‑protein phosphotransferase of PTS system (enzyme I)
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (48/64);  total (48/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

Only 100 of 114 total aligned reads displayed.

AAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGC  >  NC_000913/2534109‑2534300
                                                                                                 |                                                                                              
aaGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGc                                                                                              <  2:1587183/100‑1 (MQ=255)
aaGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGc                                                                                              <  1:1918137/100‑1 (MQ=255)
 aGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGcc                                                                                             >  1:1467999/1‑100 (MQ=255)
 aGCTCTGCTTCTGAAAGAAGACGAAATTGTCAAAAAAAAAAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGcc                                                                                             <  1:836204/100‑1 (MQ=255)
  gCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCa                                                                                            >  1:1793577/1‑100 (MQ=255)
    tctGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAg                                                                                          <  2:126583/100‑1 (MQ=255)
     ctGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAgg                                                                                         >  2:86984/1‑100 (MQ=255)
     ctGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAgg                                                                                         >  2:1176319/1‑100 (MQ=255)
     ctGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAgg                                                                                         <  2:3259368/100‑1 (MQ=255)
      tGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGc                                                                                        <  2:1360609/100‑1 (MQ=255)
       gCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAgg                                                                                         >  1:1228262/1‑98 (MQ=255)
       gCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCa                                                                                       >  2:2636/1‑100 (MQ=255)
          tCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCa                                                                                    <  1:136588/100‑1 (MQ=255)
           cTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGATCGTTTTCTGAGCGGTCGCGCCAAGGCATCAg                                                                                   <  2:2339242/100‑1 (MQ=255)
            tGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGc                                                                                  >  1:783391/1‑100 (MQ=255)
            tGAAAGAAGACGAAATTGTCATTGACAGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGc                                                                                  <  1:3296197/100‑1 (MQ=255)
             gAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGcc                                                                                 <  2:3059977/100‑1 (MQ=255)
             gAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGcc                                                                                 >  2:3177938/1‑100 (MQ=255)
               aagaagACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCa                                                                               >  1:2284759/1‑100 (MQ=255)
                agaagaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAg                                                                              <  2:1038599/100‑1 (MQ=255)
                  aagaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCt                                                                            >  1:1218420/1‑100 (MQ=255)
                  aagaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCt                                                                            >  1:3393543/1‑100 (MQ=255)
                  aagaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCt                                                                            <  2:2678871/100‑1 (MQ=255)
                    gaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTgg                                                                          <  2:2713441/100‑1 (MQ=255)
                    gaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTgg                                                                          <  2:2713279/100‑1 (MQ=255)
                    gaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTgg                                                                          <  1:2690439/100‑1 (MQ=255)
                    gaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTgg                                                                          <  2:1130026/100‑1 (MQ=255)
                     aCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGa                                                                         <  2:175565/100‑1 (MQ=255)
                     aCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGa                                                                         >  2:3040609/1‑100 (MQ=255)
                      cGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGaa                                                                        <  2:2139038/100‑1 (MQ=255)
                      cGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGaa                                                                        <  2:3403281/100‑1 (MQ=255)
                      cGAAATTGTCATTGACCGGAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGaaa                                                                       >  1:889606/1‑100 (MQ=255)
                        aaaTTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGaaa                                                                       >  2:2786582/1‑99 (MQ=255)
                        aaaTTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAAc                                                                      >  2:570904/1‑100 (MQ=255)
                          aTTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGa                                                                    >  2:185467/1‑100 (MQ=255)
                           ttGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGAt                                                                   >  2:674931/1‑100 (MQ=255)
                            tGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATc                                                                  <  2:422958/100‑1 (MQ=255)
                             gTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCa                                                                 <  1:2660942/100‑1 (MQ=255)
                             gTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCa                                                                 <  2:2968197/100‑1 (MQ=255)
                              tCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCaa                                                                <  1:1659166/100‑1 (MQ=255)
                              tCATTGACCGGAAAAAAATTTCTGCCGACCAGGGTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGAt                                                                   <  1:2786538/97‑1 (MQ=255)
                               cATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCaaa                                                               >  1:3211460/1‑100 (MQ=255)
                                aTTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCaaaa                                                              <  2:2746432/100‑1 (MQ=255)
                                 ttGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAAc                                                             <  2:2175792/100‑1 (MQ=255)
                                  tGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACg                                                            <  1:1185386/100‑1 (MQ=255)
                                     ccGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGaaa                                                         <  1:3177886/100‑1 (MQ=255)
                                     ccGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGaaa                                                         >  2:1339659/1‑100 (MQ=255)
                                      cGGAAAAAAATTTCTGCCGCCCAGTTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAg                                                        <  2:866157/100‑1 (MQ=255)
                                        gAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTGTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCt                                                      >  1:1924289/1‑100 (MQ=255)
                                          aaaaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTgg                                                    >  1:3459979/1‑100 (MQ=255)
                                            aaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTg                                                  <  2:387297/100‑1 (MQ=255)
                                            aaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTg                                                  <  2:1990072/100‑1 (MQ=255)
                                             aaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGa                                                 <  2:377021/100‑1 (MQ=255)
                                                tttCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAAc                                              <  2:1735539/100‑1 (MQ=255)
                                                 ttCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACg                                             >  1:3179672/1‑100 (MQ=255)
                                                    tGCCGACGAGGTGGATCAGGGAGTTGGACGTTTTGTTAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGAGCAAAACGAAAGCTGGTGAAACGTTc                                          <  1:1717094/100‑1 (MQ=255)
                                                     gCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGtcggcagaaat                                         >  2:632560/1‑90 (MQ=255)
                                                     gCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCg                                         >  1:330064/1‑100 (MQ=255)
                                                      ccGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCgg                                        <  2:2523679/100‑1 (MQ=255)
                                                      ccGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCAGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCgg                                        <  1:1313/100‑1 (MQ=255)
                                                       cGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGt                                       <  2:1666935/100‑1 (MQ=255)
                                                       cGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGt                                       <  1:1845802/100‑1 (MQ=255)
                                                          ccAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTgaa                                    <  2:1464260/100‑1 (MQ=255)
                                                              gTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGaaa                                >  2:3130512/1‑100 (MQ=255)
                                                                tGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGaaaaa                              >  1:1536232/1‑100 (MQ=255)
                                                                tGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGaaaaa                              >  2:884564/1‑100 (MQ=255)
                                                                tGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGaaaaa                              >  1:2101924/1‑100 (MQ=255)
                                                                tGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGaaaaa                              >  1:302025/1‑100 (MQ=255)
                                                                 gATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAaag                             <  2:649293/100‑1 (MQ=255)
                                                                 gATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGACAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAaag                             <  2:163756/100‑1 (MQ=255)
                                                                  agcAGGAAGGGGAACGTTTTATGAGCGGGCGGGGAAAGGCATCAGCCCAGGTGGAAAGGATCAAAACGAAAGCTGGGGAAACGTTCGGTGAAGAAAaaga                            <  1:1308749/98‑1 (MQ=255)
                                                                  aTCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAaaga                            <  1:3086285/100‑1 (MQ=255)
                                                                    cAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAaagaag                          <  2:378335/100‑1 (MQ=255)
                                                                     aGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAaagaag                          >  1:3093779/1‑99 (MQ=255)
                                                                     aGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGc                         <  1:1537688/100‑1 (MQ=255)
                                                                     aGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGc                         <  1:3065957/100‑1 (MQ=255)
                                                                     aGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGc                         <  1:230148/100‑1 (MQ=255)
                                                                     aGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGc                         <  1:3065964/100‑1 (MQ=255)
                                                                      ggAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGcc                        >  1:185859/1‑100 (MQ=255)
                                                                          gTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGGTGGAAACGATCAAAACGAACGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCt                    <  1:551735/100‑1 (MQ=255)
                                                                          gTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCt                    <  1:2605155/100‑1 (MQ=255)
                                                                           ttGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCtt                   <  2:3258741/100‑1 (MQ=255)
                                                                            tGAACGTTTTCTGAGCGGTCGGGCCAAGTCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCttt                  >  2:318324/1‑100 (MQ=255)
                                                                            tGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCttt                  >  1:2850676/1‑100 (MQ=255)
                                                                            tGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCttt                  >  2:1898623/1‑100 (MQ=255)
                                                                            tGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCttt                  >  1:229072/1‑100 (MQ=255)
                                                                             gAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTg                 >  2:3163561/1‑100 (MQ=255)
                                                                             gAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTg                 >  1:2512858/1‑100 (MQ=255)
                                                                              aaCGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGa                <  2:2489942/100‑1 (MQ=255)
                                                                                 gTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAgg             <  2:2850720/100‑1 (MQ=255)
                                                                                   tttCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGc           <  1:1149068/100‑1 (MQ=255)
                                                                                      cTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCata        >  1:303084/1‑100 (MQ=255)
                                                                                       tGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCatat       >  2:1295069/1‑100 (MQ=255)
                                                                                         aGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCAtattn     <  2:255611/100‑2 (MQ=255)
                                                                                         aGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCAtatta     <  2:257544/100‑1 (MQ=255)
                                                                                            ggTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtgc  <  1:3058777/100‑1 (MQ=255)
                                                                                            ggTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtgc  <  2:3109995/100‑1 (MQ=255)
                                                                                            ggTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtgc  <  2:2284791/100‑1 (MQ=255)
                                                                                             gTCGGGCCAAGGCATCAGCCCAGCTGGAGACGATAAAAACGAGAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATACTAtgc  <  1:1673725/99‑1 (MQ=255)
                                                                                             gTCGGGCCAAGGCATCAGCACAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtgc  <  1:2526935/99‑1 (MQ=255)
                                                                                                 |                                                                                              
AAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGC  >  NC_000913/2534109‑2534300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: