Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,593,917 G→A A648V (GCG→GTG)  tmcA ← elongator methionine tRNA (ac4C34) acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,593,9170GA98.2% 357.8 / ‑2.9 111A648V (GCG→GTG) tmcAelongator methionine tRNA (ac4C34) acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (50/58);  total (53/58)
Fisher's exact test for biased strand distribution p-value = 2.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

Only 100 of 111 total aligned reads displayed.

CCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTGT  >  NC_000913/2593822‑2594012
                                                                                               |                                                                                               
ccATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTg                                                                                             <  2:2568746/100‑1 (MQ=255)
  aTGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTgcg                                                                                           >  1:2718211/1‑100 (MQ=255)
  aTGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTgcg                                                                                           >  2:3460108/1‑100 (MQ=255)
    gagaTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGcgcg                                                                                         >  2:2279191/1‑100 (MQ=255)
     agaTAAAATTGAAGGAGTCAATGAAATAATTGCCATTGAATTATGCGATCCCGCAGACACCCAGCGCGCACAACATTAACTGCGAACAGCCCCTccgc                                                                                          >  1:905149/1‑98 (MQ=255)
      gaTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCgg                                                                                        >  1:1881863/1‑99 (MQ=255)
      gaTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGc                                                                                       >  2:1710933/1‑100 (MQ=255)
      gaTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGAGTTGTCCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTgc                                                                                            >  1:1902963/1‑95 (MQ=255)
       aTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGcc                                                                                      <  1:1415309/100‑1 (MQ=255)
       aTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGcc                                                                                      <  2:3431921/100‑1 (MQ=255)
       aTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGcc                                                                                      <  2:3433378/100‑1 (MQ=255)
       aTAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGcc                                                                                      >  1:3371856/1‑100 (MQ=255)
         aaaaTTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCctc                                                                                    >  2:748976/1‑100 (MQ=255)
           aaTTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCtt                                                                                  >  1:2871671/1‑100 (MQ=255)
             ttGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTcc                                                                                >  2:110961/1‑100 (MQ=255)
              tGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTccc                                                                               >  1:2971638/1‑100 (MQ=255)
               gAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCg                                                                              >  2:3226779/1‑100 (MQ=255)
                 aGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCt                                                                            >  1:3124227/1‑100 (MQ=255)
                  ggAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTg                                                                           >  2:1465698/1‑100 (MQ=255)
                   gAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGa                                                                          >  2:2600016/1‑100 (MQ=255)
                    aGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGAc                                                                         <  2:2390652/100‑1 (MQ=255)
                     gTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACg                                                                        <  2:1856845/100‑1 (MQ=255)
                         gTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGAcc                                                                    >  2:3303408/1‑100 (MQ=255)
                         gTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGAcc                                                                    <  2:1081255/100‑1 (MQ=255)
                         gTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGAcc                                                                    >  2:78530/1‑100 (MQ=255)
                           gAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAg                                                                  <  1:2835664/100‑1 (MQ=255)
                           gAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAg                                                                  <  1:1540835/100‑1 (MQ=255)
                           gAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAg                                                                  >  1:2411529/1‑100 (MQ=255)
                           gAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAg                                                                  <  1:1299775/100‑1 (MQ=255)
                           gAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAg                                                                  <  2:1914912/100‑1 (MQ=255)
                             aataatTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTa                                                                >  1:2823073/1‑100 (MQ=255)
                             aataatTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTa                                                                <  1:3326332/100‑1 (MQ=255)
                                aatTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACa                                                             <  2:2777467/100‑1 (MQ=255)
                                aatTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACa                                                             >  1:735805/1‑100 (MQ=255)
                                aatTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACa                                                             >  2:177076/1‑100 (MQ=255)
                                 atTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACAt                                                            >  2:677908/1‑100 (MQ=255)
                                   tGCCATTGCGTTATGCGATCACGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCt                                                          >  1:2120121/1‑100 (MQ=255)
                                    gCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCtt                                                         >  2:50893/1‑100 (MQ=255)
                                    gCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCtt                                                         >  1:2535970/1‑100 (MQ=255)
                                     ccATGGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCAGTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGAACAGTAACATCTTg                                                        <  2:777437/100‑1 (MQ=255)
                                      cATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGc                                                       <  2:486586/100‑1 (MQ=255)
                                      cATTGCGTTATGCGATCGCGCAGACGCGCAGGGCGAACATCATTAAGTGGGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGc                                                       >  2:1514893/1‑100 (MQ=255)
                                       aTTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCg                                                      <  1:1615846/100‑1 (MQ=255)
                                        ttGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCgg                                                     <  2:1573/100‑1 (MQ=255)
                                         tGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGc                                                    <  1:1310218/100‑1 (MQ=255)
                                            gTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTg                                                 <  1:2471824/100‑1 (MQ=255)
                                              tATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGaa                                               <  2:1784821/100‑1 (MQ=255)
                                              tATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGaa                                               <  2:1414762/100‑1 (MQ=255)
                                               aTGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACCTCTTGCGGCCTGaa                                               >  1:3361262/1‑99 (MQ=255)
                                               aTGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGaaa                                              >  1:422385/1‑100 (MQ=255)
                                               aTGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGaaa                                              >  1:2890320/1‑100 (MQ=255)
                                               aTGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGaaa                                              <  1:3226727/100‑1 (MQ=255)
                                                tGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTGCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAg                                             <  1:1856854/100‑1 (MQ=255)
                                                  cGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGtt                                           >  1:385983/1‑100 (MQ=255)
                                                   gATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGttt                                          >  2:2100894/1‑100 (MQ=255)
                                                   gATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGttt                                          <  1:178611/100‑1 (MQ=255)
                                                   gATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGttt                                          >  2:653328/1‑100 (MQ=255)
                                                       gcgcAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAgt                                      >  1:921886/1‑100 (MQ=255)
                                                        cgcAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAgtg                                     >  1:2103909/1‑100 (MQ=255)
                                                          cAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGtgtg                                   <  2:825827/100‑1 (MQ=255)
                                                          cAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGtgtg                                   >  1:715112/1‑100 (MQ=255)
                                                           aGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTgg                                  >  2:2749418/1‑100 (MQ=255)
                                                           aGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTgg                                  <  1:1465676/100‑1 (MQ=255)
                                                             aCGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTa                                <  1:835508/100‑1 (MQ=255)
                                                              cGCTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTAc                               <  2:1702215/100‑1 (MQ=255)
                                                                cTCAGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACAt                             <  1:1437083/100‑1 (MQ=255)
                                                                   aGTGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAAc                          <  2:821684/100‑1 (MQ=255)
                                                                     tGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACt                         >  1:1619196/1‑99 (MQ=255)
                                                                     tGCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTg                        >  2:2389917/1‑100 (MQ=255)
                                                                      gCGAACTTCATTAAGTGCGAACAGCAGCTGCGAGTGCACTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGCGCTAc                               <  1:2290606/92‑1 (MQ=255)
                                                                      gCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTgc                       <  2:3144904/100‑1 (MQ=255)
                                                                      gCGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTg                        <  1:396373/99‑1 (MQ=255)
                                                                       cGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTgcg                      <  1:2376656/100‑1 (MQ=255)
                                                                       cGAACATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTgcg                      >  1:297806/1‑100 (MQ=255)
                                                                         aaCATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTgcac                     <  2:2349721/99‑3 (MQ=255)
                                                                         aaCATCATTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCa                    <  2:3204520/100‑1 (MQ=255)
                                                                            atcatTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCAt                   <  1:748708/98‑1 (MQ=255)
                                                                              catTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACt               >  2:3233907/1‑100 (MQ=255)
                                                                              catTAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACt               >  1:87148/1‑100 (MQ=255)
                                                                                ttAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTgg             <  2:2022077/100‑1 (MQ=255)
                                                                                 tAAGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGc            <  1:1188675/100‑1 (MQ=255)
                                                                                  aaGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCg           >  2:1461417/1‑100 (MQ=255)
                                                                                   aGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCg           >  1:82726/1‑99 (MQ=255)
                                                                                   aGTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGt          >  2:3214946/1‑100 (MQ=255)
                                                                                    gTGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGtt         >  1:566736/1‑100 (MQ=255)
                                                                                     tGCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTTAAAGAATAAGTGTGGTACATAACTGCGCATCACTGGCGttt        >  2:1166840/1‑100 (MQ=255)
                                                                                      gCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGtttt       <  1:2383656/100‑1 (MQ=255)
                                                                                      gCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGtttt       >  2:1096302/1‑100 (MQ=255)
                                                                                      gCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGtttt       <  1:732388/100‑1 (MQ=255)
                                                                                      gCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGtttt       >  1:653251/1‑100 (MQ=255)
                                                                                      gCGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGtttt       <  1:3213569/100‑1 (MQ=255)
                                                                                       cGAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGttttt      >  1:3393462/1‑100 (MQ=255)
                                                                                        gAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTc     <  2:1881890/100‑1 (MQ=255)
                                                                                        gAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTc     >  1:2704633/1‑100 (MQ=255)
                                                                                        gAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTc     <  2:1319702/100‑1 (MQ=255)
                                                                                        gAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTc     <  1:1984710/100‑1 (MQ=255)
                                                                                        gAACAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTc     <  1:1283715/100‑1 (MQ=255)
                                                                                         aaCAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCt    <  1:244043/100‑1 (MQ=255)
                                                                                          aCAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTg   <  1:1873732/100‑1 (MQ=255)
                                                                                           cAGCACCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTGt  <  1:2475691/100‑1 (MQ=255)
                                                                                               |                                                                                               
CCATGAGATAAAATTGAAGGAGTCAGTGAAATAATTGCCATTGCGTTATGCGATCGCGCAGACGCTCAGTGCGAACATCATTAAGTGCGAACAGCGCCTGCGCGGCCTCTTCCCGCTGACGGACCAGTAACATCTTGCGGCCTGAAAGTTTAAGTGTGGTACATAACTGCGCATCACTGGCGTTTTTCTGT  >  NC_000913/2593822‑2594012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: