Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,810,756 G→A intergenic (+77/‑14) ygaH → / → mprA putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,810,7560GA96.7% 382.0 / 1.7 122intergenic (+77/‑14)ygaH/mprAputative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base A (61/57);  total (63/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

Only 100 of 126 total aligned reads displayed.

AAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCC  >  NC_000913/2810660‑2810851
                                                                                                |                                                                                               
 aGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGt                                                                                             <  1:792250/100‑1 (MQ=255)
 aGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGt                                                                                             <  1:626201/100‑1 (MQ=255)
   tGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCa                                                                                           <  2:1602562/100‑1 (MQ=255)
    gATGGCGATTATATAACCCACAAGAATCATTGTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCAt                                                                                          >  1:154742/1‑100 (MQ=255)
     aTGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATa                                                                                         <  2:2095565/100‑1 (MQ=255)
      nggCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTAACTGTCGTTACTATATCGGCTGAAATTAATAAg                                                                                               >  1:1678722/2‑93 (MQ=255)
       ggCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATa                                                                                         >  1:49581/1‑98 (MQ=255)
       gacGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCAt                                                                                          <  1:3211442/95‑1 (MQ=255)
        gCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATAccc                                                                                      >  2:75366/1‑100 (MQ=255)
         cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCa                                                                                     >  1:280239/1‑100 (MQ=255)
         cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCa                                                                                     <  2:151843/100‑1 (MQ=255)
         cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCa                                                                                     >  1:255143/1‑100 (MQ=255)
         cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCa                                                                                     >  1:1999697/1‑100 (MQ=255)
            ttATATAACCAACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAAt                                                                                  <  1:852847/100‑1 (MQ=255)
               tataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGa                                                                               >  2:700763/1‑100 (MQ=255)
               tataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGa                                                                               >  1:2358618/1‑100 (MQ=255)
                ataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGAt                                                                              >  2:366137/1‑100 (MQ=255)
                ataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGAt                                                                              <  2:364942/100‑1 (MQ=255)
                ataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGAt                                                                              <  1:3051618/100‑1 (MQ=255)
                    cccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTTACTGTCTTTTCTATATCGGCTGTAATTGTTAAGGTCATAGCAAAATGAATAGtt                                                                          >  1:3284798/1‑100 (MQ=255)
                        caAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGtt                                                                      <  1:2061777/100‑1 (MQ=255)
                          aGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTa                                                                    <  1:2623352/100‑1 (MQ=255)
                           gAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTAc                                                                   >  2:1603923/1‑100 (MQ=255)
                            aaTCATTTTTCTAAAACAATACATTTGCTTTATTTGTCACTGTCGTTACTATATTGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACg                                                                  <  1:820734/100‑1 (MQ=255)
                            aaTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACg                                                                  <  1:1738990/100‑1 (MQ=255)
                               cATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGccc                                                               >  1:2504108/1‑100 (MQ=255)
                               cATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGccc                                                               >  1:1228782/1‑100 (MQ=255)
                                aTTTTTCTACAGCAATACATTCACTTTATTCGTCACTGTCGTTACTATATCGGCTAAAATTAATAAGCTCATAACCAAATGGACCGTTCGTTTAc                                                                   >  1:819802/1‑95 (MQ=255)
                                aTTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCa                                                              >  2:2197010/1‑100 (MQ=255)
                                   tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                           <  1:3405733/100‑1 (MQ=255)
                                   tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                           <  2:3177353/100‑1 (MQ=255)
                                    ttCTAAAACAATACATTTACTTTATTTGTCACAGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                           >  2:3294626/1‑99 (MQ=255)
                                      cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGaa                                                         >  1:2997915/1‑99 (MQ=255)
                                      cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                        <  1:2722770/100‑1 (MQ=255)
                                        aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaa                                                      >  1:31160/1‑100 (MQ=255)
                                        aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaa                                                      >  2:245890/1‑100 (MQ=255)
                                         aaaCAATACATTTACTTTATTTGTCACTTTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaaa                                                     <  2:639365/100‑1 (MQ=255)
                                         aaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaaa                                                     >  1:3112209/1‑100 (MQ=255)
                                          aaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAAt                                                    <  2:1569305/100‑1 (MQ=255)
                                          aaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAAt                                                    <  1:2969167/100‑1 (MQ=255)
                                           aCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATg                                                   <  1:208486/100‑1 (MQ=255)
                                             aaTACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCt                                                 >  1:2565254/1‑100 (MQ=255)
                                             aaTACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATAACCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCt                                                 >  1:136638/1‑100 (MQ=255)
                                              aTACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCt                                                 <  2:1028115/99‑1 (MQ=255)
                                              aTACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTa                                                <  2:1138939/100‑1 (MQ=255)
                                                aCATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaa                                              >  1:636857/1‑100 (MQ=255)
                                                  acttACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAt                                            <  1:2111740/98‑1 (MQ=255)
                                                  aTTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAt                                            <  1:2634665/100‑1 (MQ=255)
                                                    ttACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAt                                            >  1:3281831/1‑98 (MQ=255)
                                                     tACTTTATTTGTAACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTc                                         >  2:753706/1‑100 (MQ=255)
                                                      aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                        <  2:1920997/100‑1 (MQ=255)
                                                      aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                        <  2:2895293/100‑1 (MQ=255)
                                                      aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                        >  1:1920968/1‑100 (MQ=255)
                                                      aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                        >  1:1463236/1‑100 (MQ=255)
                                                       cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                       <  2:2603743/100‑1 (MQ=255)
                                                       cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                       >  2:1682720/1‑100 (MQ=255)
                                                       cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                       >  1:3205372/1‑100 (MQ=255)
                                                       cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                       <  2:304477/100‑1 (MQ=255)
                                                        tttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgcg                                      >  1:86017/1‑100 (MQ=255)
                                                         ttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACACATGCTAAAATTTCgcgc                                     >  1:1633528/1‑100 (MQ=255)
                                                         ttAATTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCgcgc                                     <  2:1230746/100‑1 (MQ=255)
                                                          tATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGcc                                    <  1:3409619/100‑1 (MQ=255)
                                                          tATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGcc                                    >  1:983473/1‑100 (MQ=255)
                                                           aTTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCa                                   >  1:10296/1‑100 (MQ=255)
                                                            tttGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAg                                  >  2:1892096/1‑100 (MQ=255)
                                                                tCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccgc                              <  2:2602958/100‑1 (MQ=255)
                                                                tCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccgc                              <  1:1757781/100‑1 (MQ=255)
                                                                 cACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccgcc                             >  1:2215189/1‑100 (MQ=255)
                                                                 cACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccgcc                             <  2:3258961/100‑1 (MQ=255)
                                                                 cACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccgcc                             >  2:1674275/1‑100 (MQ=255)
                                                                  aCTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCa                            <  2:2504141/100‑1 (MQ=255)
                                                                   cTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTATAATTTCGCGCCAGCCGCCAc                           <  1:2128165/100‑1 (MQ=255)
                                                                     gTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGa                         >  1:90677/1‑100 (MQ=255)
                                                                     gTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGa                         >  2:2558716/1‑100 (MQ=255)
                                                                     gTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGa                         >  1:3238963/1‑100 (MQ=255)
                                                                        gTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAg                       <  1:1686856/99‑1 (MQ=255)
                                                                        gTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGa                      >  2:2103955/1‑100 (MQ=255)
                                                                            cTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtttt                  >  1:2509045/1‑100 (MQ=255)
                                                                             tataTCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACCAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAttt                   >  1:3268412/1‑98 (MQ=255)
                                                                             tataTCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTc                 <  1:3396050/100‑1 (MQ=255)
                                                                             tataTCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTc                 <  2:2598888/100‑1 (MQ=255)
                                                                              gatctggCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                <  2:1767064/95‑1 (MQ=255)
                                                                              atatCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                <  1:1190188/100‑1 (MQ=255)
                                                                              acatCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCGAGCCGCCACGAAGATTTTcc                <  1:2116852/98‑1 (MQ=255)
                                                                                atCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCtt              <  2:435116/100‑1 (MQ=255)
                                                                                 tCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTa             <  1:1007881/100‑1 (MQ=255)
                                                                                 tCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTa             >  1:36837/1‑100 (MQ=255)
                                                                                  cGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTAt            >  1:2982454/1‑100 (MQ=255)
                                                                                   ggCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTccagatc           >  1:1767039/1‑95 (MQ=255)
                                                                                   ggCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc           >  2:2176934/1‑100 (MQ=255)
                                                                                    gCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAACTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCa          >  1:779681/1‑100 (MQ=255)
                                                                                      tGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg        <  2:2534222/100‑1 (MQ=255)
                                                                                      tGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg        >  1:2067698/1‑100 (MQ=255)
                                                                                      tGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg        <  2:1463258/100‑1 (MQ=255)
                                                                                        aaaTTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCACATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgag      >  1:1318057/1‑100 (MQ=255)
                                                                                           ttAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATc   >  1:3051424/1‑100 (MQ=255)
                                                                                            taataaGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATcc  >  1:575808/1‑100 (MQ=255)
                                                                                            taataaGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATcc  >  1:1641627/1‑100 (MQ=255)
                                                                                            taataaGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATcc  <  1:601617/100‑1 (MQ=255)
                                                                                            taataaGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACAACGCTTTTCCTTATCAGGAGATcc  >  1:2904043/1‑100 (MQ=255)
                                                                                                |                                                                                               
AAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCC  >  NC_000913/2810660‑2810851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: