Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,966,414 T→G D674A (GAT→GCT)  ptsP ← PEP‑protein phosphotransferase enzyme I; GAF domain containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,966,4140TG97.7% 429.2 / 0.0 131D674A (GAT→GCT) ptsPPEP‑protein phosphotransferase enzyme I; GAF domain containing protein
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base G (48/78);  total (50/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

Only 100 of 131 total aligned reads displayed.

TGCGCCGCAGCAGGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGAC  >  NC_000913/2966319‑2966510
                                                                                               |                                                                                                
tGCGCCGCAGCAGGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGcgcc                                                                                              <  1:690880/100‑1 (MQ=255)
tGCGCCGCAGCAGGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGcgcc                                                                                              >  2:2473941/1‑100 (MQ=255)
 gcgcCGCAGCAGGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGcgccc                                                                                             <  2:2380435/100‑1 (MQ=255)
   gccgcAGCAGGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGCTTGCCACGCACATGGGAGcgcccgc                                                                                           >  1:2049792/1‑100 (MQ=255)
   gccgcAGCAGGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGcgcccgc                                                                                           <  2:303885/100‑1 (MQ=255)
      gcagcaGGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCAt                                                                                        >  1:124265/1‑100 (MQ=255)
         gcagGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTc                                                                                     <  2:2714520/100‑1 (MQ=255)
            ggTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCAcc                                                                                  <  2:2095681/100‑1 (MQ=255)
            ggTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCAcc                                                                                  <  1:3339056/100‑1 (MQ=255)
             gTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCg                                                                                 <  1:3090091/100‑1 (MQ=255)
             gTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCg                                                                                 >  2:2498127/1‑100 (MQ=255)
              tATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGc                                                                                <  2:255877/100‑1 (MQ=255)
              tATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGc                                                                                <  2:619819/100‑1 (MQ=255)
               aTTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGca                                                                               >  2:3074342/1‑100 (MQ=255)
                  ttGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACaa                                                                            >  1:1885547/1‑100 (MQ=255)
                    gCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAAc                                                                          <  2:1009246/100‑1 (MQ=255)
                      ccGCGCTACAGAACGTCCGTTCATAGACATATGGCGATACCCAAGCCCAAAGAGGATTGCCACGCACATGCGAGCGCCCGCCATTTCACCGCACAAACg                                                                         >  1:100899/1‑99 (MQ=255)
                      ccGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACgg                                                                        >  2:3204682/1‑100 (MQ=255)
                      ccGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACg                                                                         <  1:439518/99‑1 (MQ=255)
                       cgcgCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGga                                                                       <  1:1834220/100‑1 (MQ=255)
                          gcTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGAt                                                                    <  2:816334/100‑1 (MQ=255)
                            tACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCg                                                                  <  1:756159/100‑1 (MQ=255)
                             aCAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCg                                                                  >  1:2566710/1‑99 (MQ=255)
                             aCAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGa                                                                 >  2:1950708/1‑100 (MQ=255)
                              cAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGAt                                                                <  1:2733341/100‑1 (MQ=255)
                              cAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGAt                                                                <  1:2912465/100‑1 (MQ=255)
                                gAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTc                                                              >  2:974339/1‑100 (MQ=255)
                                gAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTc                                                              <  1:229/100‑1 (MQ=255)
                                 aaCGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTcc                                                             <  2:591621/100‑1 (MQ=255)
                                  aCGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCa                                                            >  2:2416094/1‑100 (MQ=255)
                                  aCGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCa                                                            >  1:1867391/1‑100 (MQ=255)
                                   cGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCAt                                                           >  1:2042086/1‑100 (MQ=255)
                                     tCCGTTCATAGACAGCTGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGt                                                         <  2:3430781/100‑1 (MQ=255)
                                     tCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGt                                                         <  1:3255046/100‑1 (MQ=255)
                                     tCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGt                                                         >  1:1413588/1‑100 (MQ=255)
                                     tCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGt                                                         >  1:573424/1‑100 (MQ=255)
                                      ccGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTa                                                        <  2:678045/100‑1 (MQ=255)
                                      ccGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTa                                                        <  2:1893966/100‑1 (MQ=255)
                                        gTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTAtt                                                      <  2:551758/100‑1 (MQ=255)
                                           cATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTcc                                                   <  2:2794321/100‑1 (MQ=255)
                                           cATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTcc                                                   <  2:1581120/100‑1 (MQ=255)
                                             tAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGc                                                 <  1:2010241/100‑1 (MQ=255)
                                             tAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGc                                                 <  1:1037068/100‑1 (MQ=255)
                                                aCAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTc                                              <  2:293465/100‑1 (MQ=255)
                                                 cAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTcc                                             >  2:915886/1‑100 (MQ=255)
                                                 cAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTc                                              >  1:2114367/1‑99 (MQ=255)
                                                 cAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTc                                              >  1:2893215/1‑99 (MQ=255)
                                                   gATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCg                                           >  2:1957711/1‑100 (MQ=255)
                                                   gATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCg                                           >  2:2621076/1‑100 (MQ=255)
                                                    aTGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCgg                                          <  2:124267/100‑1 (MQ=255)
                                                      ggCGTTACCCAAGCCCAATGTGGATTGCCACGCTCGTGGGAGGGCCCTGCATTTCACCGCGCAAGCGGAGATCGATTCCATGTATTTCCGCTTCCCGGGc                                        <  1:687699/100‑1 (MQ=255)
                                                      ggCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGc                                        <  1:2954964/100‑1 (MQ=255)
                                                      ggCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGc                                        >  2:400250/1‑100 (MQ=255)
                                                       gCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCg                                       <  1:2310030/100‑1 (MQ=255)
                                                       gCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCg                                       <  2:1055817/100‑1 (MQ=255)
                                                       gCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCg                                       >  2:1189567/1‑100 (MQ=255)
                                                        cGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGa                                      >  2:2292200/1‑100 (MQ=255)
                                                         gATACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGat                                     >  1:934342/1‑100 (MQ=255)
                                                           tACCCAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGatca                                   <  2:682852/100‑1 (MQ=255)
                                                             cccAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGatcatc                                 >  1:3129320/1‑100 (MQ=255)
                                                             cccAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGatcatc                                 >  1:1289903/1‑100 (MQ=255)
                                                              ccAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACTGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCg                                <  1:1904031/100‑1 (MQ=255)
                                                              ccAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCg                                <  2:790152/100‑1 (MQ=255)
                                                              ccAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCg                                <  1:932959/100‑1 (MQ=255)
                                                               cAAGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGc                               <  1:482818/100‑1 (MQ=255)
                                                                aaGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGcc                              <  1:1058816/100‑1 (MQ=255)
                                                                aaGCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGcc                              <  2:1011048/100‑1 (MQ=255)
                                                                  gCCCAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCag                            <  2:859514/100‑1 (MQ=255)
                                                                   cccAACGAGGATTGCCACGCACACGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCag                            <  2:1564600/99‑1 (MQ=255)
                                                                    ccAATGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCagag                          <  1:2191518/100‑1 (MQ=255)
                                                                      aaTGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCt                        <  1:1759641/100‑1 (MQ=255)
                                                                      aaTGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCt                        >  2:1523085/1‑100 (MQ=255)
                                                                      aaTGAGGATTGCCACGCACATGGGAGCACCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGc                         >  2:1191353/1‑99 (MQ=255)
                                                                       aTGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTc                       <  2:403952/100‑1 (MQ=255)
                                                                        tGAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCg                      >  1:3244189/1‑100 (MQ=255)
                                                                         gAGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGt                     <  1:3264883/100‑1 (MQ=255)
                                                                          aGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTa                    <  2:934354/100‑1 (MQ=255)
                                                                          aGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTa                    >  1:962532/1‑100 (MQ=255)
                                                                          aGGATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTa                    <  1:3004505/100‑1 (MQ=255)
                                                                          aGGATTGCCACACACATGGAAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCAATGTATTTCCGCTTCCCGGGCGACCATCGCCAGAGCTCGTa                    >  1:1337026/1‑100 (MQ=255)
                                                                           ggATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTaa                   <  2:821458/100‑1 (MQ=255)
                                                                            gATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAAc                  <  2:2410327/100‑1 (MQ=255)
                                                                            gATTGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAAc                  <  1:974327/100‑1 (MQ=255)
                                                                              ttcccACTCACATGGGACCTCCCGCCATTTCACCGCACAAACTGAGATCAATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTaa                   <  1:1589024/94‑1 (MQ=255)
                                                                              ttGCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACAt                <  1:681139/100‑1 (MQ=255)
                                                                                gCCACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTg              <  2:1282054/100‑1 (MQ=255)
                                                                                 ccACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCGGGGCGATCATCGCCAGAGCTCGTAACATTGc             <  1:1184945/100‑1 (MQ=255)
                                                                                  cACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCa            <  2:1316732/100‑1 (MQ=255)
                                                                                  cACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCa            <  1:3357911/100‑1 (MQ=255)
                                                                                  cACGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCa            >  2:1296408/1‑100 (MQ=255)
                                                                                  cACGCACATGGGAGCGCCCGCAATTTCACCGCAAAAACGGAGATCGATTCGATGTATTTCAGCTTCCCGGGCGATCATAGCAACAGCTCGTAACATTGc             >  1:330350/1‑99 (MQ=255)
                                                                                  cACGCACATGGGAACGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCa            >  1:266711/1‑100 (MQ=255)
                                                                                   gatctcaTGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAg           <  2:2871520/95‑1 (MQ=255)
                                                                                    cGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATGATCGCCAGAGCTCGTAACATTGCAgg          >  1:1245222/1‑100 (MQ=255)
                                                                                    cGCACATGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAgg          <  2:2219559/100‑1 (MQ=255)
                                                                                        caTGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAgagatc      >  1:2871474/1‑95 (MQ=255)
                                                                                        caTGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGa      >  2:2520685/1‑100 (MQ=255)
                                                                                         aTGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGaa     <  2:3351284/100‑1 (MQ=255)
                                                                                          tGGGAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAg    >  2:2736669/1‑100 (MQ=255)
                                                                                            ggAGCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGAc  <  1:1058594/100‑1 (MQ=255)
                                                                                               |                                                                                                
TGCGCCGCAGCAGGTATTTTGCCCGCGCTACAGAACGTCCGTTCATAGACAGATGGCGATACCCAAGCCCAATGAGGATTGCCACGCACATGGGATCGCCCGCCATTTCACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGAC  >  NC_000913/2966319‑2966510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: