Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,165,015 T→A D320V (GAT→GTT)  parC ← DNA topoisomerase IV, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,165,0150TA99.2% 422.5 / NA 129D320V (GAT→GTT) parCDNA topoisomerase IV, subunit A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (69/59);  minor base C (0/1);  total (69/60)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

CACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATT  >  NC_000913/3164918‑3165111
                                                                                                 |                                                                                                
cacCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACg                                                                                                >  1:1322575/1‑100 (MQ=255)
    aGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggtgg                                                                                            <  2:2668448/100‑1 (MQ=255)
    aGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggtgg                                                                                            <  1:3091529/100‑1 (MQ=255)
     gCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggtggt                                                                                           >  1:1011525/1‑100 (MQ=255)
     gCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggtggt                                                                                           <  1:321463/100‑1 (MQ=255)
       cATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTa                                                                                          >  1:1178251/1‑99 (MQ=255)
       cATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAg                                                                                         >  1:161069/1‑100 (MQ=255)
       cATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGAAGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCGTTTCCAGAACggtg                                                                                             >  1:2633124/1‑96 (MQ=255)
           cGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGaa                                                                                     >  2:1488182/1‑100 (MQ=255)
            ggAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGCGGTAGCGac                                                                                     >  1:1033525/1‑98 (MQ=255)
             gagaGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAaga                                                                                   <  1:879971/100‑1 (MQ=255)
              agagGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAagag                                                                                  >  2:1002848/1‑100 (MQ=255)
              agagGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAagag                                                                                  <  1:3147439/100‑1 (MQ=255)
              agagGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAagag                                                                                  <  2:2857552/100‑1 (MQ=255)
               gagGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAgg                                                                                 >  1:63803/1‑100 (MQ=255)
                 ggATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAagag                                                                                  >  1:1352261/1‑97 (MQ=255)
                 ggATTTCCAGCAGATTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtg                                                                               >  2:2334922/1‑100 (MQ=255)
                   aTTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtggt                                                                             <  2:576509/100‑1 (MQ=255)
                     ttCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtc                                                                           <  1:905450/100‑1 (MQ=255)
                      tCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtca                                                                          <  1:1968290/100‑1 (MQ=255)
                       ccAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtcat                                                                         >  2:1689550/1‑100 (MQ=255)
                        cagcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtcatc                                                                        <  1:1589948/100‑1 (MQ=255)
                        cagcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtcatc                                                                        >  1:440882/1‑100 (MQ=255)
                         agcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtcatca                                                                       >  2:2725327/1‑100 (MQ=255)
                          gcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATATGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCAc                                                                      <  1:2347469/100‑1 (MQ=255)
                          gcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCAc                                                                      <  2:3096766/100‑1 (MQ=255)
                          gcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAAAACGGTGGTAGCGAAGAGGTGGCTCATCAc                                                                      >  1:2985913/1‑100 (MQ=255)
                           cagGTTTTTCATCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCAc                                                                      >  1:1991193/1‑99 (MQ=255)
                           cagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCAcc                                                                     >  2:3282203/1‑100 (MQ=255)
                           cagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCAcc                                                                     <  2:1065355/100‑1 (MQ=255)
                             ggTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTg                                                                   <  1:40481/100‑1 (MQ=255)
                              gTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGa                                                                  >  1:2619028/1‑100 (MQ=255)
                               tttttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGAt                                                                 <  2:155946/100‑1 (MQ=255)
                                ttttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATc                                                                <  2:2082500/100‑1 (MQ=255)
                                ttttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATc                                                                <  2:1841440/100‑1 (MQ=255)
                                  ttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTTTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCa                                                              <  1:1928663/100‑1 (MQ=255)
                                  ttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCa                                                              <  1:1469005/100‑1 (MQ=255)
                                  ttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGAGGTAGCGAAGAGGTGGTTCATCACCTGATCCa                                                              >  1:2902099/1‑100 (MQ=255)
                                    cACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCata                                                            <  1:1696020/100‑1 (MQ=255)
                                    cACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCata                                                            >  2:3348631/1‑100 (MQ=255)
                                    cACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCata                                                            <  2:1771997/100‑1 (MQ=255)
                                    cACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCata                                                            <  2:3197642/100‑1 (MQ=255)
                                    cACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCata                                                            <  1:2642028/100‑1 (MQ=255)
                                     aCCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCatat                                                           <  2:2015195/100‑1 (MQ=255)
                                      ccgccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATc                                                          >  2:52518/1‑100 (MQ=255)
                                      ccgccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGAGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATc                                                          <  2:2450137/100‑1 (MQ=255)
                                       cgccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATcc                                                         >  2:1136408/1‑100 (MQ=255)
                                       cgccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATcc                                                         >  1:2655907/1‑100 (MQ=255)
                                       cgccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATcc                                                         >  1:3256119/1‑100 (MQ=255)
                                       cgccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATcc                                                         <  1:1838859/100‑1 (MQ=255)
                                            gacgacCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGATGTGGTTCATCACCTGATCCATATCCAcgcg                                                    <  1:1003107/100‑1 (MQ=255)
                                            gacgacCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCAcgcg                                                    >  2:874837/1‑100 (MQ=255)
                                            gacgacCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCAcgcg                                                    <  2:372706/100‑1 (MQ=255)
                                              cgacCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGt                                                  >  1:2547666/1‑100 (MQ=255)
                                              cgacCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGt                                                  >  1:2452439/1‑100 (MQ=255)
                                                acCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTg                                                <  2:2043732/100‑1 (MQ=255)
                                                 ccATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTgg                                               >  2:3344909/1‑100 (MQ=255)
                                                  cATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGa                                              <  2:320424/100‑1 (MQ=255)
                                                    tCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAAc                                            <  2:171855/100‑1 (MQ=255)
                                                        gACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCgg                                        <  1:2100233/100‑1 (MQ=255)
                                                         aCCGATCATATTAAGGTTAATACGATAGCTCTTTTCCCGACCGGTGGTAGCGAAGAGCTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGc                                       <  2:2771165/100‑1 (MQ=255)
                                                         aCCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGc                                       <  1:874825/100‑1 (MQ=255)
                                                          ccGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGca                                      >  2:2696474/1‑100 (MQ=255)
                                                           cGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGcac                                     >  2:2682494/1‑100 (MQ=255)
                                                            gATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGcaca                                    >  1:2813950/1‑100 (MQ=255)
                                                             aTCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCTCCTGATCCATATCCACGCGGTTGGAACGCGGCACaa                                   <  2:2455101/100‑1 (MQ=255)
                                                              tCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAAt                                  >  2:407057/1‑100 (MQ=255)
                                                                 tatTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCAc                               >  2:3361433/1‑100 (MQ=255)
                                                                 tatTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCAc                               <  1:407213/100‑1 (MQ=255)
                                                                 tatTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGATGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCAc                               >  1:111577/1‑100 (MQ=255)
                                                                  atTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCAcc                              <  2:3170785/100‑1 (MQ=255)
                                                                  atTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCAcc                              >  1:2711232/1‑100 (MQ=255)
                                                                     aaGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAgg                           >  2:842804/1‑100 (MQ=255)
                                                                       ggTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcg                         >  2:487468/1‑100 (MQ=255)
                                                                         ttAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcggg                       <  1:3353624/100‑1 (MQ=255)
                                                                          tAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACTAGGcgggt                      >  2:3081591/1‑100 (MQ=255)
                                                                          tAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcggg                       >  1:1560550/1‑99 (MQ=255)
                                                                          tAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcggg                       >  1:978133/1‑99 (MQ=255)
                                                                            aTACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtn                     <  1:572578/99‑2 (MQ=255)
                                                                            aTACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcg                    >  2:3450072/1‑100 (MQ=255)
                                                                             tACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcgg                   >  1:1286391/1‑100 (MQ=255)
                                                                              aCGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcggg                  <  2:192103/100‑1 (MQ=255)
                                                                              aCGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcggg                  <  2:1352280/100‑1 (MQ=255)
                                                                               cGATTGCTCTCTTCCAGGACGGTGGTAGCGAAGAGGTGGTTGATCACCTTATCCATATCCACGCGGTTGGTACGGGGCACAATCACCAGGcgcgtcggg                  <  1:2407423/99‑1 (MQ=255)
                                                                               cGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcgggt                 <  1:2331182/100‑1 (MQ=255)
                                                                               cGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcgggt                 <  1:1981179/100‑1 (MQ=255)
                                                                                  tAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTtct              >  2:2731481/1‑100 (MQ=255)
                                                                                  tAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTtct              <  1:1136393/100‑1 (MQ=255)
                                                                                    gCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCg            <  1:690534/100‑1 (MQ=255)
                                                                                    gCTCTTTTCCAGAACGGTGGTAGCGAAGAAGTGGTTCATCACCTGATCCATATCCACGCGGTTGGATCGCGGCACAATCACCAGGCGGGTCGGGTTCTCg            <  1:2095902/100‑1 (MQ=255)
                                                                                     ctctTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGt           >  2:2310072/1‑100 (MQ=255)
                                                                                     ctctTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGt           <  2:824510/100‑1 (MQ=255)
                                                                                     ctccTTTCCAGAACGGTGGTAGCGGAGGGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGTcggttcgggt                 >  1:832384/1‑94 (MQ=255)
                                                                                       ctTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTgg         >  2:2762714/1‑100 (MQ=255)
                                                                                        ttttCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGt        <  2:2411136/100‑1 (MQ=255)
                                                                                         tttCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTc       >  1:1753888/1‑100 (MQ=255)
                                                                                         tttCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTc       >  2:57971/1‑100 (MQ=255)
                                                                                          ttCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCa      >  1:3382169/1‑100 (MQ=255)
                                                                                            ccAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGa    >  1:1306131/1‑100 (MQ=255)
                                                                                             cAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGAt   <  2:2202088/100‑1 (MQ=255)
                                                                                                 |                                                                                                
CACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATT  >  NC_000913/3164918‑3165111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑