Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,350,648 T→A intergenic (‑189/+41) elbB ← / ← arcB isoprenoid biosynthesis protein with amidotransferase‑like domain/aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,350,6480TA97.9% 465.8 / ‑0.6 142intergenic (‑189/+41)elbB/arcBisoprenoid biosynthesis protein with amidotransferase‑like domain/aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
Reads supporting (aligned to +/- strand):  ref base T (0/3);  new base A (68/69);  total (68/74)
Fisher's exact test for biased strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 142 total aligned reads displayed.

CATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCC  >  NC_000913/3350551‑3350744
                                                                                                 |                                                                                                  
cATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAgg                                                                                                  <  2:1318881/100‑1 (MQ=255)
 aTTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGgt                                                                                                 >  2:1711533/1‑100 (MQ=255)
  tttAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGgtg                                                                                                <  2:423218/100‑1 (MQ=255)
   ttAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGgtgt                                                                                               >  1:292042/1‑100 (MQ=255)
    tAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGtt                                                                                             <  1:1235090/100‑1 (MQ=255)
      aGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTgg                                                                                           <  1:1032854/100‑1 (MQ=255)
      aGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTgg                                                                                           <  2:328501/100‑1 (MQ=255)
         tttGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGgcgc                                                                                        <  2:2222785/100‑1 (MQ=255)
         tttGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGgcgc                                                                                        <  1:420393/100‑1 (MQ=255)
           tGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAg                                                                                      <  2:3202931/100‑1 (MQ=255)
           tGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAg                                                                                      >  1:3274801/1‑100 (MQ=255)
            gCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAAATCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGACGCAGt                                                                                     >  1:141653/1‑100 (MQ=255)
             cTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTa                                                                                    >  2:2174748/1‑100 (MQ=255)
              tATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTAt                                                                                   >  2:948860/1‑100 (MQ=255)
               aTCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTAtt                                                                                  >  1:2895134/1‑100 (MQ=255)
                tCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAACCAAGATTTCCCAGGTGTTGGCGCAGTATTc                                                                                 >  2:121583/1‑100 (MQ=255)
                  ttAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTcgc                                                                               <  1:2587256/100‑1 (MQ=255)
                   tAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTcgcg                                                                              >  2:1309965/1‑100 (MQ=255)
                   tAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTcgcg                                                                              <  2:1730788/100‑1 (MQ=255)
                   tAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTcgcg                                                                              <  1:1524998/100‑1 (MQ=255)
                      cTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCAc                                                                           >  2:192057/1‑100 (MQ=255)
                          gtgcgGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCg                                                                       >  2:1346842/1‑100 (MQ=255)
                             cgGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTc                                                                    <  1:2327079/100‑1 (MQ=255)
                             cgGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTc                                                                    <  1:3012239/100‑1 (MQ=255)
                                ccTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAg                                                                 <  2:3063898/100‑1 (MQ=255)
                                 cTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGc                                                               >  1:2705707/1‑100 (MQ=255)
                                  tGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGcc                                                              >  1:1318469/1‑100 (MQ=255)
                                  tGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGcc                                                              >  2:757850/1‑100 (MQ=255)
                                   gAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCg                                                             >  2:1531064/1‑100 (MQ=255)
                                    aaaaaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCgg                                                            <  1:50856/100‑1 (MQ=255)
                                    aaaaaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCgg                                                            <  1:2391332/100‑1 (MQ=255)
                                    aaaaaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCgg                                                            >  1:519132/1‑100 (MQ=255)
                                    aaaaaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTCGGCGCAGTATTCGCGCACCCCGGTCTAGCCgg                                                            <  2:2234523/100‑1 (MQ=255)
                                     aaaaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCggg                                                           >  1:1025019/1‑100 (MQ=255)
                                     aaaaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCggg                                                           <  2:1279066/100‑1 (MQ=255)
                                     aaaaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCggg                                                           >  2:3454455/1‑100 (MQ=255)
                                      aaaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTACTCGCGCACCCCGGTCTAGCCgggg                                                          >  1:2862893/1‑100 (MQ=255)
                                         cAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGCTAGACCGGGGtgc                                                       <  2:2030969/100‑3 (MQ=255)
                                          aGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCCGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGCTAGACCGGGGtgcg                                                      <  1:1407230/100‑4 (MQ=255)
                                          aGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGCTAGACCGGGGtgcg                                                      <  1:2538916/100‑4 (MQ=255)
                                          aGTGCTGTGCCCTTGTAACTCATCATAAGAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGCTAGACCGGGGtgcg                                                      <  2:1156284/100‑4 (MQ=255)
                                            tGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTTGCCGGGGtgcgcg                                                    <  2:2356110/100‑6 (MQ=255)
                                             gCTGTGCCCTTGTAACTCATCATAATATTTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCAtttt                                                   <  2:1189548/100‑1 (MQ=255)
                                             gCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCAtttt                                                   >  1:3472974/1‑100 (MQ=255)
                                             gCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCAtttt                                                   >  1:196328/1‑100 (MQ=255)
                                              cTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGCTCTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGCTAGACCGGGGtgcgcgaa                                                  <  1:3309785/100‑8 (MQ=37)
                                              cTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGCTAGACCGGGGtgcgcgaa                                                  <  2:2151223/100‑8 (MQ=38)
                                               tgtgCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCAtttttt                                                 <  2:2787978/100‑1 (MQ=255)
                                               tgtgCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTGCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCAtttttt                                                 <  2:2831192/100‑1 (MQ=255)
                                                gtgCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTa                                                <  2:1193233/100‑1 (MQ=255)
                                                gtgCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTa                                                <  1:1844089/100‑1 (MQ=255)
                                                gtgCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTa                                                <  2:1608297/100‑1 (MQ=255)
                                                 tgCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAg                                               <  2:188203/100‑1 (MQ=255)
                                                  gCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGt                                              <  2:977177/100‑1 (MQ=255)
                                                  gCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGt                                              >  1:2491594/1‑100 (MQ=255)
                                                    ccTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTgg                                            >  1:3081312/1‑100 (MQ=255)
                                                    ccTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTgg                                            >  2:602379/1‑100 (MQ=255)
                                                       tGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCtt                                         <  1:1961139/100‑1 (MQ=255)
                                                       tGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCtt                                         >  2:2953532/1‑100 (MQ=255)
                                                          aaCTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTg                                      >  2:251370/1‑100 (MQ=255)
                                                          aaCTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTg                                      <  1:102400/100‑1 (MQ=255)
                                                           aCTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGc                                     <  1:1811650/100‑1 (MQ=255)
                                                           aCTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGc                                     >  2:672123/1‑100 (MQ=255)
                                                            cTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGcc                                    <  2:2041652/100‑1 (MQ=255)
                                                             tcatcaTAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGcca                                   <  2:3177115/100‑1 (MQ=255)
                                                              catcatAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGccac                                  >  2:2575278/1‑100 (MQ=255)
                                                              catcatAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGccac                                  <  1:17649/100‑1 (MQ=255)
                                                               atcatAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGccacc                                 >  1:2213028/1‑100 (MQ=255)
                                                               atcatAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGccacc                                 >  1:2212929/1‑100 (MQ=255)
                                                               atcatAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGccacc                                 <  1:173780/100‑1 (MQ=255)
                                                                tcatAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGccaccc                                >  2:3144777/1‑100 (MQ=255)
                                                                tcatAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGccaccc                                <  1:2184326/100‑1 (MQ=255)
                                                                  ataataATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGccacccac                              >  1:2532250/1‑100 (MQ=255)
                                                                   taataaTTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACg                             <  1:3209679/100‑1 (MQ=255)
                                                                   taataaTTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACg                             >  1:3068587/1‑100 (MQ=255)
                                                                        atTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTc                        <  1:968486/100‑1 (MQ=255)
                                                                        atTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTc                        >  2:399317/1‑100 (MQ=255)
                                                                         tttACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCa                       <  1:2953485/100‑1 (MQ=255)
                                                                         tttACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCa                       >  2:184051/1‑100 (MQ=255)
                                                                          ttACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAg                      <  2:1086956/100‑1 (MQ=255)
                                                                          ttACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAg                      <  2:3473030/100‑1 (MQ=255)
                                                                             cGGGGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGACATCCACGCTTTCAGCAc                   <  1:831915/100‑1 (MQ=255)
                                                                              ggCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACt                  >  2:3444685/1‑100 (MQ=255)
                                                                              ggCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACt                  >  2:3020009/1‑100 (MQ=255)
                                                                              ggCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACt                  <  2:3068637/100‑1 (MQ=255)
                                                                              ggCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACACACGCTTTCAGCACt                  >  2:582859/1‑100 (MQ=255)
                                                                                cgcAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTc                <  1:3237917/100‑1 (MQ=255)
                                                                                 gcAGCTAAGACTTCCCAGTGTGTGGCGCAGTATTCGCTCTCCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGc                     <  1:3224445/94‑1 (MQ=255)
                                                                                   aGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTAc             >  1:1896633/1‑100 (MQ=255)
                                                                                   aGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCACTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTAc             >  1:771908/1‑100 (MQ=255)
                                                                                    gCCAAGATTTCCCCGGTGTGGGCGCTGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTc                <  1:2274789/96‑1 (MQ=255)
                                                                                    gCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTAcg            >  2:605606/1‑100 (MQ=255)
                                                                                      cAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTAcgtc          >  2:1303151/1‑100 (MQ=255)
                                                                                       aaGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTAcgtcg         <  1:2248204/100‑1 (MQ=255)
                                                                                       aaGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTAcgtcg         <  2:2543538/100‑1 (MQ=255)
                                                                                       aaGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTAcgtcg         <  2:295725/100‑1 (MQ=255)
                                                                                        aGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTAcgtcgt        >  1:1626447/1‑100 (MQ=255)
                                                                                         gATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTg       <  2:2375871/100‑1 (MQ=255)
                                                                                              cccAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGcc  >  2:2133531/1‑100 (MQ=255)
                                                                                              cccAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTAGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGcc  <  1:157516/100‑1 (MQ=255)
                                                                                                 |                                                                                                  
CATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCC  >  NC_000913/3350551‑3350744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: