Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,443,086 T→A N338Y (AAC→TAC)  secY ← preprotein translocase membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,443,0860TA99.3% 509.5 / ‑5.2 149N338Y (AAC→TAC) secYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (66/81);  total (68/81)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 149 total aligned reads displayed.

ATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAAT  >  NC_000913/3442989‑3443182
                                                                                                 |                                                                                                
aTTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCTGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGa                                                                                                <  1:1859631/100‑1 (MQ=255)
  tACTTTATCGACATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGaaa                                                                                              <  1:2860688/100‑1 (MQ=255)
   aCTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGaaaa                                                                                             <  2:2647402/100‑1 (MQ=255)
    cTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAAc                                                                                            <  2:1158985/100‑1 (MQ=255)
     tttATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAAc                                                                                            <  1:1436265/99‑1 (MQ=255)
      ttATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCa                                                                                          <  1:855682/100‑1 (MQ=255)
           gATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTCGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGcc                                                                                     >  1:1867791/1‑100 (MQ=255)
               taCTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCgtgt                                                                                 <  1:1767971/100‑1 (MQ=255)
                aCTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTa                                                                                <  2:2500137/100‑1 (MQ=255)
                 cTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTag                                                                               >  1:2792101/1‑100 (MQ=255)
                 cTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTag                                                                               <  2:1121604/100‑1 (MQ=255)
                   tCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTagaa                                                                             >  2:346374/1‑100 (MQ=255)
                    cGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTagaag                                                                            <  1:846519/100‑1 (MQ=255)
                     gCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTagaaga                                                                           <  2:2268510/100‑1 (MQ=255)
                     gCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTagaaga                                                                           >  2:155377/1‑100 (MQ=255)
                      ccGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAgaagaa                                                                          <  2:188406/100‑1 (MQ=255)
                      ccGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAgaagaa                                                                          <  2:912483/100‑1 (MQ=255)
                        gTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAAc                                                                        >  1:3086537/1‑100 (MQ=255)
                          ttGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACag                                                                      <  1:866991/100‑1 (MQ=255)
                           tGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACaga                                                                     >  2:2647403/1‑100 (MQ=255)
                           tGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACaga                                                                     <  1:42551/100‑1 (MQ=255)
                           tGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACaga                                                                     <  2:2031589/100‑1 (MQ=255)
                            gCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGAAGAAAACCAACGCCGTGTAGAAGAAACagaa                                                                    >  2:2940459/1‑100 (MQ=255)
                             ctctCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACagaag                                                                   >  1:2891484/1‑100 (MQ=255)
                             ctctCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACagaag                                                                   >  2:2486313/1‑100 (MQ=255)
                              tctcCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACagaaga                                                                  <  1:465806/100‑1 (MQ=255)
                               ctcCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACagaagaa                                                                 <  1:223447/100‑1 (MQ=255)
                                tcCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACagaagaag                                                                >  2:3174844/1‑100 (MQ=255)
                                 cccGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACagaagaaga                                                               <  2:2905275/100‑1 (MQ=255)
                                  ccGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAgat                                                              >  1:1094239/1‑100 (MQ=255)
                                  ccGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAgat                                                              <  2:2084832/100‑1 (MQ=255)
                                   cGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAgatg                                                             >  1:1269665/1‑100 (MQ=255)
                                   cGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAgatg                                                             >  2:2764910/1‑100 (MQ=255)
                                    ggACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAgatga                                                            >  2:461205/1‑100 (MQ=255)
                                     gACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAgatgat                                                           <  1:247092/100‑1 (MQ=255)
                                      aCGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGAtt                                                          >  1:906800/1‑100 (MQ=255)
                                       cGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTg                                                         >  2:1395587/1‑100 (MQ=255)
                                       cGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTg                                                         <  2:740481/100‑1 (MQ=255)
                                              cTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGc                                                  <  2:1025367/100‑1 (MQ=255)
                                              cTGGTACAAATGCACCGGACTTCTTCAGGTTAGCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGc                                                  <  1:1575350/100‑1 (MQ=255)
                                               tGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCa                                                 >  2:1013555/1‑100 (MQ=255)
                                                  tACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATag                                              <  2:183106/100‑1 (MQ=255)
                                                  tACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATag                                              <  2:200918/100‑1 (MQ=255)
                                                  tACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATag                                              <  1:3193083/100‑1 (MQ=255)
                                                   aCAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATag                                              <  1:2166725/99‑1 (MQ=255)
                                                    cAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATagag                                            <  1:784689/100‑1 (MQ=255)
                                                    cAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATagag                                            <  1:3174793/100‑1 (MQ=255)
                                                     aaaTGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGt                                           <  2:3379921/100‑1 (MQ=255)
                                                     aaaTGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGt                                           <  1:3206233/100‑1 (MQ=255)
                                                      aaTGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGCAGAAGATGATTGCAGACGAATAGAGTa                                          >  1:1083987/1‑100 (MQ=255)
                                                      aaTGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTa                                          <  1:37954/100‑1 (MQ=255)
                                                        tGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAACCAGAAGAAGATGATTGCAGACGCATAGAGTAac                                        >  2:797141/1‑100 (MQ=255)
                                                         gCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAaca                                       <  1:1424742/100‑1 (MQ=255)
                                                            cctgACTGCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACAt                                    <  1:2641965/97‑1 (MQ=255)
                                                            ccGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACAt                                    >  2:1151269/1‑100 (MQ=255)
                                                            ccGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACAt                                    >  2:2546029/1‑100 (MQ=255)
                                                             cGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATa                                   >  2:3365301/1‑100 (MQ=255)
                                                             cGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATa                                   <  2:2681011/100‑1 (MQ=255)
                                                               gACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAATATGATTGCAGACGCATACAGTAACACATaaa                                 <  2:2336256/100‑1 (MQ=255)
                                                               gACTTCTTCAGGTTATCTGCTGTTTAACACGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATATAGTAACACATaaa                                 <  1:3010325/100‑1 (MQ=255)
                                                                aCTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAg                                <  1:981288/100‑1 (MQ=255)
                                                                 cttcttCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGc                               >  2:142924/1‑100 (MQ=255)
                                                                   tcttcAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCgg                             >  2:554040/1‑100 (MQ=255)
                                                                     ttcAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGtt                           <  1:492263/100‑1 (MQ=255)
                                                                     ttcAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGtt                           <  2:2715265/100‑1 (MQ=255)
                                                                      tcAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTg                          >  1:428865/1‑100 (MQ=255)
                                                                      tcAGGTTATCCGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTg                          >  2:325942/1‑100 (MQ=255)
                                                                        aGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGcc                        <  2:1985328/100‑1 (MQ=255)
                                                                        aGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGcc                        <  2:3114891/100‑1 (MQ=255)
                                                                        aGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGcc                        >  1:1744107/1‑100 (MQ=255)
                                                                        aGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGcc                        <  1:860965/100‑1 (MQ=255)
                                                                         ggTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGccc                       >  1:363679/1‑100 (MQ=255)
                                                                         ggTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGccc                       >  1:1689971/1‑100 (MQ=255)
                                                                         ggTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGc                         >  1:1043203/1‑98 (MQ=255)
                                                                          gTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGccc                       >  2:2218805/1‑99 (MQ=255)
                                                                           ttATCCGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAg                     <  1:325938/100‑1 (MQ=255)
                                                                             aTCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGc                   <  2:15332/100‑1 (MQ=255)
                                                                               ctgctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTg                 <  2:1357889/100‑1 (MQ=255)
                                                                                tgctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGc                >  2:75154/1‑100 (MQ=255)
                                                                                tgctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGc                <  1:2169951/100‑1 (MQ=255)
                                                                                 gctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCa               >  1:2623972/1‑100 (MQ=255)
                                                                                 gctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCa               <  1:423606/100‑1 (MQ=255)
                                                                                 gctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCa               <  2:1867818/100‑1 (MQ=255)
                                                                                 gctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACAAAAAGCGGTTGCCCAGGCTGCa               >  1:752690/1‑100 (MQ=255)
                                                                                  ctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCaa              <  2:2211279/100‑1 (MQ=255)
                                                                                      ttCACGCGGGTAGAAAACCAACGCCGCGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATAc          >  2:213327/1‑100 (MQ=255)
                                                                                       tCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACa         >  1:2953771/1‑100 (MQ=255)
                                                                                       tCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACa         <  2:1174708/100‑1 (MQ=255)
                                                                                        cACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAg        >  2:3394753/1‑100 (MQ=255)
                                                                                        cACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAg        >  1:282155/1‑100 (MQ=255)
                                                                                        cACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAg        <  1:3116434/100‑1 (MQ=255)
                                                                                         aCGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGc       <  2:906812/100‑1 (MQ=255)
                                                                                          cgcgGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCg      <  1:553339/100‑1 (MQ=255)
                                                                                          cgcgGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCg      <  1:253407/100‑1 (MQ=255)
                                                                                          cgcgGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCg      >  1:1290083/1‑100 (MQ=255)
                                                                                          cgcgGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCg      <  2:807415/100‑1 (MQ=255)
                                                                                          cgcgGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACAAAAAGCGGTTGCCCAGGCTGCAAATACAGCg      >  1:793458/1‑100 (MQ=255)
                                                                                            cgGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGaa    >  1:233092/1‑100 (MQ=255)
                                                                                             gggTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGaaa   >  2:2594308/1‑100 (MQ=255)
                                                                                              ggTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAAt  >  1:747680/1‑100 (MQ=255)
                                                                                                 |                                                                                                
ATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAAT  >  NC_000913/3442989‑3443182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: