Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,541,962 T→G I519S (ATC→AGC)  feoB → ferrous iron transporter protein B and GTP‑binding protein; membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,541,9620TG94.1% 394.4 / 4.1 119I519S (ATC→AGC) feoBferrous iron transporter protein B and GTP‑binding protein; membrane protein
Reads supporting (aligned to +/- strand):  ref base T (2/5);  new base G (59/53);  total (61/58)
Fisher's exact test for biased strand distribution p-value = 2.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

GGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAG  >  NC_000913/3541867‑3542058
                                                                                               |                                                                                                
ggAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGagcatc                                                                                              <  2:3342198/100‑1 (MQ=255)
ggAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGagcatc                                                                                              >  2:1771846/1‑100 (MQ=255)
     tGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGt                                                                                         <  1:603973/100‑1 (MQ=255)
      gCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTTAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTc                                                                                        >  1:2445897/1‑100 (MQ=255)
      gCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTc                                                                                        >  1:262145/1‑100 (MQ=255)
        cGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAg                                                                                      <  1:3015185/100‑1 (MQ=255)
        cGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAg                                                                                      <  2:2907762/100‑1 (MQ=255)
        cGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAg                                                                                      <  2:603536/100‑1 (MQ=255)
         ggTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGc                                                                                     <  1:2871314/100‑1 (MQ=255)
         ggTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGc                                                                                     <  2:1644441/100‑1 (MQ=255)
          gTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGGCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCa                                                                                    <  2:648471/100‑1 (MQ=255)
           tCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCAt                                                                                   <  2:1842919/100‑1 (MQ=255)
            cTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCAtt                                                                                  >  2:68975/1‑100 (MQ=255)
             tATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCAttt                                                                                 >  2:2732227/1‑100 (MQ=255)
              aTCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCAtttt                                                                                >  2:2879319/1‑100 (MQ=255)
              aTCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCAtttt                                                                                >  1:1018400/1‑100 (MQ=255)
               tCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTc                                                                               >  2:1289596/1‑100 (MQ=255)
                  tGTACCACACGTTAAAAGCCTGATTATCCATACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTg                                                                            <  1:345287/100‑1 (MQ=255)
                   gTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGa                                                                           >  2:1093888/1‑100 (MQ=255)
                    tACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAg                                                                          <  1:1345363/100‑1 (MQ=255)
                    tACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAg                                                                          <  2:1300733/100‑1 (MQ=255)
                    tACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAg                                                                          <  2:2122342/100‑1 (MQ=255)
                     aCCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAgc                                                                         >  1:2017803/1‑100 (MQ=255)
                     aCCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAgc                                                                         >  1:2266908/1‑100 (MQ=255)
                      ccACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAgcg                                                                        >  2:621826/1‑100 (MQ=255)
                       cacaCGTTAAAAGCCTGATTATCCAGACCTGTCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAgcg                                                                        <  2:1180161/99‑1 (MQ=255)
                       cacaCGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAgcgc                                                                       >  2:1381042/1‑100 (MQ=255)
                       cacaCGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGCCTTCGTTCTGCGTGCTGGTAAAGTGCGCATCATCGTCAGCATTTTCCTGAgcgc                                                                       >  1:1138697/1‑100 (MQ=255)
                           cGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTc                                                                   <  2:3149920/100‑1 (MQ=255)
                            gTTAAAAGCCTGATTATCCAGACCTGGCAGAGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCa                                                                  <  1:1272336/100‑1 (MQ=255)
                             ttAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCaa                                                                 >  2:171403/1‑100 (MQ=255)
                               aaaaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACa                                                               >  1:2430451/1‑100 (MQ=255)
                               aaaaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACa                                                               >  1:777146/1‑100 (MQ=255)
                                aaaGCCTGATTGTCGAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAg                                                              <  1:2015607/100‑1 (MQ=255)
                                aaaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAg                                                              >  1:109314/1‑100 (MQ=255)
                                aaaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAg                                                              >  2:3128587/1‑100 (MQ=255)
                                 aaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGc                                                             <  2:2017832/100‑1 (MQ=255)
                                 aaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGc                                                             <  1:3146661/100‑1 (MQ=255)
                                  aGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAAc                                                                <  1:1380660/96‑1 (MQ=255)
                                  aGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCt                                                            <  2:2308739/100‑1 (MQ=255)
                                   gCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCtt                                                           >  1:1721291/1‑100 (MQ=255)
                                   gCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCt                                                            <  1:377704/99‑1 (MQ=255)
                                     cTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTtct                                                         <  1:2139994/100‑1 (MQ=255)
                                     cTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCATTCAACAGCTtct                                                         <  1:2140298/100‑1 (MQ=255)
                                      tGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTtctc                                                        <  2:2550640/100‑1 (MQ=255)
                                       gATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCg                                                       >  1:326525/1‑100 (MQ=255)
                                       gATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCg                                                       >  2:1279239/1‑100 (MQ=255)
                                           aTCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGa                                                   >  1:116074/1‑100 (MQ=255)
                                             ccAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGc                                                 >  1:746752/1‑100 (MQ=255)
                                             ccAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGc                                                 >  2:1288736/1‑100 (MQ=255)
                                              cAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCg                                                >  2:3090013/1‑100 (MQ=255)
                                              cAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCg                                                <  1:2863857/100‑1 (MQ=255)
                                              cAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCg                                                >  1:1541904/1‑100 (MQ=255)
                                              cAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGCGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCg                                                >  1:1932026/1‑100 (MQ=255)
                                                   cTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGaaa                                           >  1:2196240/1‑100 (MQ=255)
                                                    tGGCAGCGTCTGAGAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCTCTTTCAACAGCTTCTCGCTGAGCGCGaa                                            <  1:992529/98‑1 (MQ=255)
                                                    tGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGaaaa                                          >  2:1063400/1‑100 (MQ=255)
                                                    tGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCCCGCTCAGCGAGaagc                                          <  2:1644346/100‑3 (MQ=255)
                                                     ggCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAt                                         >  1:1885614/1‑100 (MQ=255)
                                                     ggCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAt                                         <  1:100794/100‑1 (MQ=255)
                                                      gCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAtc                                        <  2:2662431/100‑1 (MQ=255)
                                                      gCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAtc                                        >  2:3332273/1‑100 (MQ=255)
                                                       cAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCGTCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAtcg                                       <  1:2441051/100‑1 (MQ=255)
                                                       cAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAtcg                                       >  2:1241074/1‑100 (MQ=255)
                                                       cAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAtcg                                       <  2:1541926/100‑1 (MQ=255)
                                                       cAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCCCGCTCAGCGAGaagctgt                                       <  2:3282993/100‑6 (MQ=255)
                                                         gCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAtcgtc                                     <  1:310151/100‑1 (MQ=255)
                                                         gCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAtcgtc                                     <  1:1013992/100‑1 (MQ=255)
                                                           gTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGCGGATCATCGTCAGCATTTTCCTGAGCGCGTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGa                                   >  1:1852615/1‑100 (MQ=255)
                                                           gTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGa                                   <  2:3478013/100‑1 (MQ=255)
                                                            tCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGGGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGAt                                  <  2:3261903/100‑1 (MQ=255)
                                                            tCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGAt                                  >  2:1913090/1‑100 (MQ=255)
                                                            tCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGAt                                  <  1:2000492/100‑1 (MQ=255)
                                                             cTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATTGTCGATa                                 <  1:1068504/100‑1 (MQ=255)
                                                             cTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATa                                 >  2:1844784/1‑100 (MQ=255)
                                                             cTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATa                                 >  1:370238/1‑100 (MQ=255)
                                                              tGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATaa                                >  1:2711874/1‑100 (MQ=255)
                                                              tGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATaa                                >  2:2182886/1‑100 (MQ=255)
                                                               gAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAAc                               >  2:896599/1‑100 (MQ=255)
                                                               gAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAAc                               >  1:2191049/1‑100 (MQ=255)
                                                                aaaGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACa                              <  2:777157/100‑1 (MQ=255)
                                                                 aaGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACAt                             >  2:1795555/1‑100 (MQ=255)
                                                                 aaGGCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACAt                             <  2:3297216/100‑1 (MQ=255)
                                                                   ggCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCa                           >  2:684371/1‑100 (MQ=255)
                                                                    gCTTCGTTCTGCGTGCTGGTAAAGTGAGCATCAACGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCTCTGAGCGGGAAAATCGTCGATAACATCaa                          >  1:2085399/1‑100 (MQ=255)
                                                                        cGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGAc                      <  2:2223034/100‑1 (MQ=255)
                                                                        cGTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGAc                      >  1:3271161/1‑100 (MQ=255)
                                                                         gTTCTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACt                     >  1:2722911/1‑100 (MQ=255)
                                                                            cTGCGTGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCgg                  <  1:78439/100‑1 (MQ=255)
                                                                                 tGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTg             >  2:3171798/1‑100 (MQ=255)
                                                                                 tGCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTg             <  1:1909756/100‑1 (MQ=255)
                                                                                  gCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTgg            <  1:2016002/100‑1 (MQ=255)
                                                                                  gCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTgg            >  1:1387417/1‑100 (MQ=255)
                                                                                  gCTGGTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTgg            >  1:1062034/1‑100 (MQ=255)
                                                                                    tGGTAAAGTGAGCATCATCGTCAGCATTTTGCTCAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTAGGCGCTGGcg          >  1:2277104/1‑100 (MQ=255)
                                                                                     ggTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcg          >  2:2071796/1‑99 (MQ=255)
                                                                                      gTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtc        <  1:2057835/100‑1 (MQ=255)
                                                                                      gTAAAGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtc        <  2:2623091/100‑1 (MQ=255)
                                                                                        aaaGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtccg      >  1:2116632/1‑100 (MQ=255)
                                                                                        aaaGTGAGCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtccg      >  2:3358139/1‑100 (MQ=255)
                                                                                               |                                                                                                
GGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAG  >  NC_000913/3541867‑3542058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑