Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 236,541 C→T R159* (CGA→TGA)  dnaQ → DNA polymerase III epsilon subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913236,5410CT98.3% 388.9 / ‑4.5 118R159* (CGA→TGA) dnaQDNA polymerase III epsilon subunit
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (56/60);  total (57/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

GGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGA  >  NC_000913/236444‑236635
                                                                                                 |                                                                                              
ggTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAaatga                                                                                              <  1:1750048/100‑1 (MQ=255)
 gTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGAAAACAGTAaatgaa                                                                                             >  2:2023734/1‑100 (MQ=255)
    aCCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGc                                                                                          <  1:3267551/100‑1 (MQ=255)
           gCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACgg                                                                                   >  1:2328658/1‑100 (MQ=255)
           gCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACgg                                                                                   >  2:1661703/1‑100 (MQ=255)
           gCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACg                                                                                    >  1:1803077/1‑99 (MQ=255)
           gCCTTGCGGTGGCGAGGAAAAGGTTTCCCGCTAAGCTCAACAGCCTCGATTCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACg                                                                                    <  1:2338742/99‑1 (MQ=255)
               tGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCa                                                                               >  2:1542038/1‑100 (MQ=255)
                gCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCAt                                                                              >  1:1205503/1‑100 (MQ=255)
                   gTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTAc                                                                           >  2:1234378/1‑100 (MQ=255)
                    tGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACt                                                                          <  1:965485/100‑1 (MQ=255)
                     ngCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTc                                                                         >  2:2677458/2‑100 (MQ=255)
                     ggCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTc                                                                         >  2:3224733/1‑100 (MQ=255)
                     ggCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTc                                                                         <  2:2812/100‑1 (MQ=255)
                     ggCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTc                                                                         <  2:3322941/100‑1 (MQ=255)
                      gCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCg                                                                        <  1:2833533/100‑1 (MQ=255)
                       cGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTACATGAACGCTGCACGGGGCATTACTCGa                                                                       >  1:3266875/1‑100 (MQ=255)
                       cGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGa                                                                       >  2:827049/1‑100 (MQ=255)
                       cGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGa                                                                       <  1:848054/100‑1 (MQ=255)
                        gAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGAt                                                                      >  2:1106730/1‑100 (MQ=255)
                         aGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATg                                                                     <  2:2531056/100‑1 (MQ=255)
                          ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGc                                                                    >  1:364421/1‑100 (MQ=255)
                          ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGc                                                                    >  2:428954/1‑100 (MQ=255)
                          ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCATTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGc                                                                    >  1:1852643/1‑100 (MQ=255)
                            aaaaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGccc                                                                  <  1:2623182/100‑1 (MQ=255)
                            aaaaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGcc                                                                   >  1:1491658/1‑99 (MQ=255)
                             aaaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCa                                                                 >  2:734508/1‑100 (MQ=255)
                                 gTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATc                                                             <  2:199618/100‑1 (MQ=255)
                                  tttCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATcc                                                            <  2:2159203/100‑1 (MQ=255)
                                  tttCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATcc                                                            >  1:1317088/1‑100 (MQ=255)
                                   ttCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCt                                                           <  1:1740288/100‑1 (MQ=255)
                                    tCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCtt                                                          >  1:2577639/1‑100 (MQ=255)
                                    tCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCtt                                                          >  1:2810567/1‑100 (MQ=255)
                                     cccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTg                                                         <  1:827038/100‑1 (MQ=255)
                                     cccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTg                                                         <  1:1194727/100‑1 (MQ=255)
                                     cccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTg                                                         <  1:3069879/100‑1 (MQ=255)
                                     cccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTg                                                         >  1:2759398/1‑100 (MQ=255)
                                       cGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCg                                                       <  2:867506/100‑1 (MQ=255)
                                        ggTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCgg                                                      >  2:3373633/1‑100 (MQ=255)
                                         gTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGa                                                     <  2:2069597/100‑1 (MQ=255)
                                           aaGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAg                                                   >  2:1962946/1‑100 (MQ=255)
                                           aaGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAg                                                   <  2:2419675/100‑1 (MQ=255)
                                           aaGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAg                                                   <  2:1045057/100‑1 (MQ=255)
                                            aGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCGTTGCGGAAGt                                                  >  1:2007793/1‑100 (MQ=255)
                                            aGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAg                                                   <  1:3225457/99‑1 (MQ=255)
                                            aGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGt                                                  <  1:2405689/100‑1 (MQ=255)
                                             gcgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGtt                                                 <  2:1250032/100‑1 (MQ=255)
                                             gcgcAACAGCCTCGATGCGTTATGCGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAg                                                   >  1:1683338/1‑98 (MQ=255)
                                              cgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGttt                                                >  2:2369458/1‑100 (MQ=255)
                                               gcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTa                                               <  1:2033414/100‑1 (MQ=255)
                                               gcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTa                                               >  1:3398776/1‑100 (MQ=255)
                                                cAACATCCTCTGTGCGTTATGTGCTCGCTACGAAATAGATAACAGGAAATGAACGCTGCACGTGGCATTACTCGATTCCCAGATCCTTGCAGAAATTTAt                                              >  1:1028159/1‑100 (MQ=255)
                                                 aaCAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATc                                             <  2:2816261/100‑1 (MQ=255)
                                                  aCAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCt                                            <  1:2252335/100‑1 (MQ=255)
                                                     gCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGc                                         >  1:3114298/1‑100 (MQ=255)
                                                      gcTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTg                                           >  2:654106/2‑97 (MQ=255)
                                                      ccTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCg                                        <  1:2365604/100‑1 (MQ=255)
                                                       cTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGa                                       <  2:1985161/100‑1 (MQ=255)
                                                         cGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATg                                     >  1:15107/1‑100 (MQ=255)
                                                         cGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGAACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATg                                     <  1:3205517/100‑1 (MQ=255)
                                                              cttccgatcttCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCgg                                <  2:168109/90‑1 (MQ=255)
                                                              cGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCTGAAGTTTATCTGGCGATGACCgg                                >  1:3267468/1‑100 (MQ=255)
                                                               gTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggt                               <  2:2547676/100‑1 (MQ=255)
                                                               gTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCgg                                >  1:1551741/1‑99 (MQ=255)
                                                                ttATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggtg                              <  1:3023480/100‑1 (MQ=255)
                                                                ttATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggtg                              >  1:1449034/1‑100 (MQ=255)
                                                                  aTGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggtggt                            <  2:2025920/100‑1 (MQ=255)
                                                                   tgtgCTCGCTACGAAATAGATAACAGTAAATGCACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTc                           <  2:212052/100‑1 (MQ=255)
                                                                    gtgCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCGGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCa                          >  1:2446578/1‑100 (MQ=255)
                                                                    gtgCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGGTCCTTGCGGAAGTTTATCTGGAGATGACCggtg                              >  1:2877551/1‑96 (MQ=255)
                                                                    gtgCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggtggt                            >  1:2426830/1‑98 (MQ=255)
                                                                    gtgCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCa                          <  1:428949/100‑1 (MQ=255)
                                                                     tgCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCaa                         >  1:3354550/1‑100 (MQ=255)
                                                                       cTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTACGGAAGTTTATCTGGCGATGACCGGTGGTCaaa                        >  1:1834730/1‑99 (MQ=255)
                                                                        tCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggagatcggaag                      >  1:168107/1‑90 (MQ=255)
                                                                         cGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGt                     >  2:62177/1‑100 (MQ=255)
                                                                          gCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTc                    <  2:387614/100‑1 (MQ=255)
                                                                          gCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTc                    <  2:3462838/100‑1 (MQ=255)
                                                                           cTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCg                   <  2:1355082/100‑1 (MQ=255)
                                                                              cGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATg                <  1:1571209/100‑1 (MQ=255)
                                                                                aaaTAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGc              <  2:2231948/100‑1 (MQ=255)
                                                                                   tAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCttt           >  1:2329785/1‑100 (MQ=255)
                                                                                     gATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTg         <  2:1641707/100‑1 (MQ=255)
                                                                                     gATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTg         >  2:1324267/1‑100 (MQ=255)
                                                                                     gATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTg         >  1:110883/1‑100 (MQ=255)
                                                                                     gATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTg         <  1:3467841/100‑1 (MQ=255)
                                                                                      aTAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGc        <  2:280251/100‑1 (MQ=255)
                                                                                       tAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCg       >  2:1002511/1‑100 (MQ=255)
                                                                                       tAACAGTAAATGAACGCTGCACAGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTAGATGGCTTTTGCg       >  1:2016586/1‑100 (MQ=255)
                                                                                         accgTAAATGAACGCTGCACGGGGCATTACTCGAAGCCAAGATACTTGCGGAAGTTTATCTGGCGATGACCGGTGGCCAAAAGTAGATGGCTTTTg         >  1:1009377/4‑96 (MQ=255)
                                                                                          cAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg    <  2:2992094/100‑1 (MQ=255)
                                                                                          cAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg    <  1:2231170/100‑1 (MQ=255)
                                                                                          cAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg    >  2:1394086/1‑100 (MQ=255)
                                                                                          cAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg    <  2:1138989/100‑1 (MQ=255)
                                                                                          cAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGAGGTCAAACGTCGATGGCTTTTGCGATg    >  1:2915538/1‑100 (MQ=255)
                                                                                          cAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCCGAAGTTTATCTGGCGATGACCGGTGGTCAAAGGTCGAAGGCTTTAGCGATg    >  2:2071343/1‑100 (MQ=255)
                                                                                           aGTAAATGAAGGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATgg   <  1:1577618/100‑1 (MQ=255)
                                                                                           aGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATgg   <  1:1962917/100‑1 (MQ=255)
                                                                                            gTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATgg   >  2:1663690/1‑99 (MQ=255)
                                                                                            gTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGa  <  1:1445770/100‑1 (MQ=255)
                                                                                                 |                                                                                              
GGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGA  >  NC_000913/236444‑236635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑