BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,669,920	A→G	E110G (GAA→GGA)	treF →	cytoplasmic trehalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,669,920	0	A	G	95.2%	393.8 / 2.8	124	E110G (GAA→GGA)	treF	cytoplasmic trehalase
Reads supporting (aligned to +/- strand): ref base A (2/4); new base G (58/60); total (60/64)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

TTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCT  >  NC_000913/3669823‑3670016

tttCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGgaa                                                                                                <  2:2307873/100‑1 (MQ=255)

tttCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGgaa                                                                                                <  1:1759602/100‑1 (MQ=255)

  tCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAAc                                                                                              <  1:2580491/100‑1 (MQ=255)

   cccGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAAcc                                                                                             >  1:3398028/1‑100 (MQ=255)

     cGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCAc                                                                                           <  2:1120423/100‑1 (MQ=255)

      gACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACt                                                                                          <  2:3226713/100‑1 (MQ=255)

      gACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACt                                                                                          <  2:406744/100‑1 (MQ=255)

          gcgcACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTg                                                                                      <  2:3362830/100‑1 (MQ=255)

          gcgcACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTg                                                                                      <  2:3007391/100‑1 (MQ=255)

          gcgcACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTg                                                                                      >  2:1877854/1‑100 (MQ=255)

           cgcACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTgg                                                                                     >  2:414434/1‑100 (MQ=255)

            gcACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGc                                                                                    >  1:1467843/1‑100 (MQ=255)

             cACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGc                                                                                    <  1:329868/99‑1 (MQ=255)

             cACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCt                                                                                   >  2:3124740/1‑100 (MQ=255)

             cACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCt                                                                                   >  2:1777913/1‑100 (MQ=255)

               ccTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGc                                                                                 >  1:3201121/1‑100 (MQ=255)

               ccTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGc                                                                                 >  1:2269022/1‑100 (MQ=255)

                cTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGcc                                                                                >  1:1310572/1‑100 (MQ=255)

                cTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGcc                                                                                >  2:366301/1‑100 (MQ=255)

                  aaaaTGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCgg                                                                              <  1:291238/100‑1 (MQ=255)

                  aaaaTGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCgg                                                                              <  2:2450913/100‑1 (MQ=255)

                     aTGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAgg                                                                           <  1:3120073/100‑1 (MQ=255)

                          cccGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTAc                                                                      >  1:1802339/1‑100 (MQ=255)

                            cGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTc                                                                    >  1:3152452/1‑100 (MQ=255)

                            cGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTc                                                                    >  2:902507/1‑100 (MQ=255)

                            cGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTCGAAACCACTTCTGGCTGCCGGACGGCTACTc                                                                    >  1:1547987/1‑100 (MQ=255)

                             gCTGGATATCATAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTcc                                                                   <  1:3155505/100‑1 (MQ=255)

                               tGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAg                                                                 <  2:2370509/100‑1 (MQ=255)

                                ggATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGc                                                                >  2:1708582/1‑100 (MQ=255)

                                ggATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGc                                                                >  2:266811/1‑100 (MQ=255)

                                ggATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGc                                                                >  1:1678746/1‑100 (MQ=255)

                                 gATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCg                                                               <  1:2576667/100‑1 (MQ=255)

                                 gATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCg                                                               <  2:1092726/100‑1 (MQ=255)

                                    atCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgt                                                            >  1:2693036/1‑100 (MQ=255)

                                    atCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgt                                                            <  2:2011898/100‑1 (MQ=255)

                                     tCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgta                                                           <  2:1328167/100‑1 (MQ=255)

                                     tCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgta                                                           >  2:2222865/1‑100 (MQ=255)

                                     tCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgta                                                           >  1:776479/1‑100 (MQ=255)

                                     tCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgta                                                           <  1:1165677/100‑1 (MQ=255)

                                      cTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtat                                                          >  1:1945302/1‑100 (MQ=255)

                                      cTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtat                                                          <  1:1017365/100‑1 (MQ=255)

                                      cTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtat                                                          <  2:3048727/100‑1 (MQ=255)

                                      cTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtat                                                          >  1:149647/1‑100 (MQ=255)

                                       ttAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatg                                                         <  1:3141564/100‑1 (MQ=255)

                                       ttAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatg                                                         >  2:243859/1‑100 (MQ=255)

                                       ttAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatg                                                         >  2:2559342/1‑100 (MQ=255)

                                       ttAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatg                                                         <  1:3268739/100‑1 (MQ=255)

                                       ttAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatg                                                         >  1:3115783/1‑100 (MQ=255)

                                          aTCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatgtat                                                      <  2:859234/100‑1 (MQ=255)

                                          aTCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatgtat                                                      <  2:2952645/100‑1 (MQ=255)

                                          aTCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatgtat                                                      >  2:2808122/1‑100 (MQ=255)

                                            ccGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCg                                                    >  2:3098109/1‑100 (MQ=255)

                                             ggCTACCGTCAAGTGCGCCGTCATCATGATCTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCgg                                                   >  1:1231714/2‑100 (MQ=255)

                                             cGCTACCGTAAAGAGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCg                                                    >  1:2149318/1‑99 (MQ=255)

                                                tACCGTAAAGTGCGCCGTCATCGTGTTTTTGACTTGCGCAAGCTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCg                                                    <  1:951753/96‑1 (MQ=255)

                                                 aCCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGAccc                                               >  2:2217068/1‑100 (MQ=255)

                                                   cGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGc                                             <  2:59656/100‑1 (MQ=255)

                                                   cGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGc                                             >  1:3016975/1‑100 (MQ=255)

                                                   cGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGc                                             <  2:1811238/100‑1 (MQ=255)

                                                           gcgcCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTc                                      <  1:2359632/99‑1 (MQ=255)

                                                            cgcCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTccc                                    <  1:321406/100‑1 (MQ=255)

                                                            cgcCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTccc                                    <  1:919172/100‑1 (MQ=255)

                                                              gatctaTCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTg                                  <  2:579677/95‑1 (MQ=255)

                                                              ccGTCATCGTTATTTTGACTTGCCCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCTGCAAAATTCCCTg                                  <  1:2153235/100‑1 (MQ=255)

                                                              ccGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCt                                   <  1:747271/99‑1 (MQ=255)

                                                              ccGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTg                                  >  2:3108796/1‑100 (MQ=255)

                                                              ccGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTg                                  >  2:1324722/1‑100 (MQ=255)

                                                              ccGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGAATGTATCGGACCCGCAAAATTCCCTg                                  <  1:13099/100‑1 (MQ=255)

                                                                gTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGaa                                <  1:2914951/100‑1 (MQ=255)

                                                                  cATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAag                              >  1:3118713/1‑100 (MQ=255)

                                                                  cATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAag                              >  2:2631138/1‑100 (MQ=255)

                                                                  cATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAag                              <  2:2112705/100‑1 (MQ=255)

                                                                   aTCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGagatc                             >  1:579670/1‑96 (MQ=255)

                                                                   aTCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAaga                             >  2:517968/1‑100 (MQ=255)

                                                                     cGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGc                           <  2:450130/100‑1 (MQ=255)

                                                                     cGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGc                           >  2:2699424/1‑100 (MQ=255)

                                                                     cGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGc                           <  1:1012430/100‑1 (MQ=255)

                                                                      gTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCa                          <  2:3152502/100‑1 (MQ=255)

                                                                       tGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCat                         <  1:601704/100‑1 (MQ=255)

                                                                        gATTTTGGCTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACGCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCata                        <  2:2149349/100‑1 (MQ=255)

                                                                        gATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCata                        >  1:750796/1‑100 (MQ=255)

                                                                         aTTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCatat                       <  2:3074663/100‑1 (MQ=255)

                                                                          ttttGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATc                      >  2:3290054/1‑100 (MQ=255)

                                                                            ttGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGTCCCGCAAAATTCCCTGAAAGAGCATATCg                     <  1:2296298/99‑1 (MQ=255)

                                                                                cTTGCGCGAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAg                <  1:2639734/100‑1 (MQ=255)

                                                                                cTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAACGAGCATATCGACCAg                <  1:1026047/100‑1 (MQ=255)

                                                                                cTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAg                >  1:522477/1‑100 (MQ=255)

                                                                                 ttGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGc               <  1:1679677/100‑1 (MQ=255)

                                                                                  tGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCt              >  2:3062548/1‑100 (MQ=255)

                                                                                   gcgcAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGACCATATCGACCacc               >  1:1618987/1‑96 (MQ=255)

                                                                                     gcAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCtgtg           <  2:756523/100‑1 (MQ=255)

                                                                                     gcAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCtgtg           <  2:2662020/100‑1 (MQ=255)

                                                                                      cAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTgg          <  1:3467357/100‑1 (MQ=255)

                                                                                           ttgttgGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGt     <  1:3062498/100‑1 (MQ=255)

                                                                                           ttgttgGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGt     <  1:246849/100‑1 (MQ=255)

                                                                                            tgttgGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGt     >  2:1773331/1‑99 (MQ=255)

                                                                                            tgttgGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTg    >  1:1084327/1‑100 (MQ=255)

                                                                                             gttgAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGc   <  1:81847/100‑1 (MQ=255)

                                                                                              ttgGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCt  >  1:1985625/1‑100 (MQ=255)

                                                                                              ttgGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCt  >  1:610931/1‑100 (MQ=255)

TTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCT  >  NC_000913/3669823‑3670016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`