Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,822,694 G→T G99W (GGG→TGG)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,822,6940GT94.6% 337.7 / 9.6 115G99W (GGG→TGG) spoTbifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base G (4/2);  new base T (52/54);  total (59/56)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.48e-01

Only 100 of 118 total aligned reads displayed.

GATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATC  >  NC_000913/3822597‑3822789
                                                                                                 |                                                                                               
gATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAgtgg                                                                                               <  1:2566775/100‑1 (MQ=255)
 aTGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAgtggg                                                                                              <  1:2681671/100‑1 (MQ=255)
   ggcggcGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAgtgg                                                                                               >  1:2331309/1‑97 (MQ=255)
   ggcggcGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGgtg                                                                                            <  2:3415055/100‑1 (MQ=255)
   ggcggcGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGgtg                                                                                            <  1:1282046/100‑1 (MQ=255)
   ggcggcGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGgtg                                                                                            >  1:2694755/1‑100 (MQ=255)
   ggcggcGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGgt                                                                                             >  2:2147876/1‑99 (MQ=255)
    gcggcgCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGgtgt                                                                                           >  2:2040710/1‑100 (MQ=255)
     cggcgTCGCTGCATGCCGTTAGTGAAGATGCTCTCGCCACCTGCCCGGATATGGAGCAGCGTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTc                                                                                          <  1:728556/100‑1 (MQ=255)
     cggcgCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTc                                                                                          >  1:1096859/1‑100 (MQ=255)
     cggcgCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTc                                                                                          >  2:2810294/1‑100 (MQ=255)
     cggcgCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTc                                                                                          <  1:2471948/100‑1 (MQ=255)
     cggcgCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTc                                                                                          >  1:2179680/1‑100 (MQ=255)
      ggcgCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCg                                                                                         <  2:1889530/100‑1 (MQ=255)
        cgcTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGaa                                                                                       >  2:1205728/1‑100 (MQ=255)
          ctgctgCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAAc                                                                                     >  2:1267664/1‑100 (MQ=255)
           tgctgcATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACt                                                                                    <  1:1761853/100‑1 (MQ=255)
            gctgcATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGCGGGTGTCGAAACtt                                                                                   >  1:3197123/1‑100 (MQ=255)
             ctgcATGACGTGATTGAAGATACTCCCGCCACCTCCCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTg                                                                                  <  1:1836638/100‑1 (MQ=255)
             ctgcATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTg                                                                                  <  1:485266/100‑1 (MQ=255)
             ctgcATGACGTGATTGAAGATACTCCCGCAACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTg                                                                                  <  1:2279932/100‑1 (MQ=255)
              tgcATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGa                                                                                 >  1:3232352/1‑100 (MQ=255)
              tgcATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGa                                                                                 <  1:745654/100‑1 (MQ=255)
               gcATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGAt                                                                                <  2:2780087/100‑1 (MQ=255)
               gcATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGAt                                                                                >  1:1686362/1‑100 (MQ=255)
                   gACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAAc                                                                            >  1:3409267/1‑100 (MQ=255)
                    aCGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACt                                                                           <  1:2589821/100‑1 (MQ=255)
                      gTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCa                                                                         <  1:3120032/100‑1 (MQ=255)
                       tGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCaa                                                                        <  2:1535041/100‑1 (MQ=255)
                         aTTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGt                                                                      <  1:223424/100‑1 (MQ=255)
                         aTTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTCTTTGGTAAAAGCGTCGCAGAGCTGGCAGAGTGGATGTCGAAACTTGATAAACTCAAg                                                                       >  2:1575478/1‑99 (MQ=255)
                           tGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTc                                                                    >  2:1771562/1‑100 (MQ=255)
                           tGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTc                                                                    <  1:3087440/100‑1 (MQ=255)
                            gAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCGGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTcc                                                                   >  1:2220862/1‑100 (MQ=255)
                              aGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCcgc                                                                 >  1:166425/1‑100 (MQ=255)
                               gATACTCCCGCCACCTACCAGGATATGGAACAGTTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCcgcg                                                                <  2:573156/100‑1 (MQ=255)
                               gATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCcgcg                                                                <  2:2249057/100‑1 (MQ=255)
                               gATACTCCCGCCACCTACCAGCATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCcgcg                                                                <  1:856525/100‑1 (MQ=255)
                                aTACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGa                                                               <  2:3401768/100‑1 (MQ=255)
                                  ncTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATa                                                             >  2:1995886/2‑100 (MQ=255)
                                  attCCCCCCACCTACCAGGATAGGTTACAGCTTTTTAGTAAAACCGTCGCCGAGCTGTTAGAGTGGGTGTCGAAACTTGTTAAACTCAAGTTCCGCGATa                                                             <  1:1105752/98‑1 (MQ=255)
                                  aCTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATa                                                             >  1:1960445/1‑100 (MQ=255)
                                  aCTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATa                                                             >  1:1551991/1‑100 (MQ=255)
                                  aCTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATa                                                             <  1:1771537/100‑1 (MQ=255)
                                   cTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATaa                                                            >  2:1720609/1‑100 (MQ=255)
                                   cTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCGGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGGTCCGCGATaa                                                            >  1:3048976/1‑100 (MQ=255)
                                    tCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATaag                                                           <  2:42169/100‑1 (MQ=255)
                                     cccGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATaaga                                                          <  1:634670/100‑1 (MQ=255)
                                      ccGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATaagaa                                                         >  2:1959146/1‑100 (MQ=255)
                                      ccGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATaagaa                                                         <  1:885084/100‑1 (MQ=255)
                                       cGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATaagaaa                                                        <  2:2591531/100‑1 (MQ=255)
                                        gCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATaagaaag                                                       <  2:2220894/100‑1 (MQ=255)
                                          cgtcTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAagag                                                     <  2:3232404/97‑1 (MQ=255)
                                          cACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAagag                                                     <  1:3421060/100‑1 (MQ=255)
                                           aCCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCTGCGATAAGAAAGAgg                                                    >  1:268080/1‑100 (MQ=255)
                                           aCCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAgg                                                    <  2:1365968/100‑1 (MQ=255)
                                           aCCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAgg                                                    <  1:871748/100‑1 (MQ=255)
                                           aCCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAgg                                                    <  1:2477436/100‑1 (MQ=255)
                                           aCCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAgg                                                    >  1:1094366/1‑100 (MQ=255)
                                           aCCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAgg                                                    <  2:2583346/100‑1 (MQ=255)
                                            ccTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGgc                                                   >  1:2849153/1‑100 (MQ=255)
                                             cTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCAGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGgcg                                                  >  2:3293048/1‑100 (MQ=255)
                                               aCCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCa                                                <  2:1096873/100‑1 (MQ=255)
                                                  aGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGc                                             >  2:805493/1‑100 (MQ=255)
                                                    natatGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCg                                           >  2:1093028/2‑100 (MQ=255)
                                                       atGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGaaa                                        >  1:1339840/1‑100 (MQ=255)
                                                        tGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGaaaa                                       >  1:1946618/1‑100 (MQ=255)
                                                        tGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGaaaa                                       <  2:546626/100‑1 (MQ=255)
                                                         ggAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAAc                                      <  2:1686385/100‑1 (MQ=255)
                                                         ggAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAAc                                      >  1:1585615/1‑100 (MQ=255)
                                                         ggAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAAc                                      >  1:3043340/1‑100 (MQ=255)
                                                         ggAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAAc                                      >  2:203952/1‑100 (MQ=255)
                                                          gAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACt                                     >  2:558949/1‑100 (MQ=255)
                                                               gCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGc                                <  1:3343321/100‑1 (MQ=255)
                                                                cTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCa                               <  2:1339859/100‑1 (MQ=255)
                                                                cTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCa                               <  1:1720586/100‑1 (MQ=255)
                                                                    ttGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAgat                           <  2:166427/100‑1 (MQ=255)
                                                                     tGGTAAAAGCGTCGTCGAGCTGGTAGCGAGGGTGTCGAAACTTGATAAACTCAAGTTCCGAGATAAGCAACAGACGCAGGCCGAAGACTCCCACAAgat                           >  1:2979013/1‑99 (MQ=255)
                                                                     tGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAgatg                          <  2:3076983/100‑1 (MQ=255)
                                                                     tGGTAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAAATCAAGTTCAGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAgatg                          >  1:3159586/1‑100 (MQ=255)
                                                                        tAAAAGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGAtt                       >  2:1166157/1‑100 (MQ=255)
                                                                         aaaaGCGTTCCCGAGCTGGTAGAGTGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGCCGCGCAGGCTTAAAACTTTCGCAAgatgatca                      <  1:3321856/100‑3 (MQ=255)
                                                                          aaaGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAAATTGAAAAGCTCAAGTACCGCGCTAAGAAAGAGGGACAGACAAAAAGCTATCGCAAGATGATTa                      >  1:3027307/1‑99 (MQ=255)
                                                                            aGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATgg                   >  1:3266347/1‑100 (MQ=255)
                                                                            aGCGTCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATgg                   <  1:1267647/100‑1 (MQ=255)
                                                                                tCGCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGAt               <  1:1748120/100‑1 (MQ=255)
                                                                                  gCCGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATgg             >  1:1524374/1‑100 (MQ=255)
                                                                                   ccGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGt            >  2:25631/1‑100 (MQ=255)
                                                                                   ccGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGt            >  2:1658784/1‑100 (MQ=255)
                                                                                   ccGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGt            >  1:783930/1‑100 (MQ=255)
                                                                                   ccGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGt            <  2:3409319/100‑1 (MQ=255)
                                                                                    cGAGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTg           >  1:2864166/1‑100 (MQ=255)
                                                                                      aGCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCa         <  2:1857643/100‑1 (MQ=255)
                                                                                       gCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAg        <  2:3028583/100‑1 (MQ=255)
                                                                                       gCTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAg        <  2:2887224/100‑1 (MQ=255)
                                                                                        cTGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAgg       >  2:1361510/1‑100 (MQ=255)
                                                                                         tGGTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGa      <  2:700730/100‑1 (MQ=255)
                                                                                          ggTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGat     >  1:2811226/1‑100 (MQ=255)
                                                                                          ggTAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGat     >  2:1104292/1‑100 (MQ=255)
                                                                                            tAGAGTGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGatat   >  1:1547815/1‑100 (MQ=255)
                                                                                                 |                                                                                               
GATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATC  >  NC_000913/3822597‑3822789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: