Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,950,468 G→T intergenic (+48/‑39) ilvL → / → ilvX ilvG operon leader peptide/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,950,4680GT96.8% 283.5 / 0.3 96intergenic (+48/‑39)ilvL/ilvXilvG operon leader peptide/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base T (39/51);  total (42/54)
Fisher's exact test for biased strand distribution p-value = 2.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

Only 100 of 105 total aligned reads displayed.

TGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATG  >  NC_000913/3950371‑3950562
                                                                                                  |                                                                                              
tgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGttt                                                                                              <  1:2782383/100‑1 (MQ=255)
tgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGttt                                                                                              >  1:2210185/1‑100 (MQ=255)
 gATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGtttt                                                                                             <  1:2515880/100‑1 (MQ=255)
 gATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGtttt                                                                                             >  2:2558485/1‑100 (MQ=255)
  attattATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTtttt                                                                                            >  2:3299663/1‑100 (MQ=255)
   ttattaTCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGtttttt                                                                                           <  1:3380949/100‑1 (MQ=255)
   ttattaTCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGtttttt                                                                                           >  2:1225097/1‑100 (MQ=255)
     attatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGtttttttt                                                                                         <  2:3391236/100‑1 (MQ=255)
     attatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGtttttttt                                                                                         >  2:3153806/1‑100 (MQ=255)
     attatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGtttttttt                                                                                         <  2:1154844/100‑1 (MQ=255)
     attatCCCACCGTACGGGGCTACACTTGGACGAGGAAAAGCTTACAGAACAAGCCTTACCGAAGTAAGACCCCCGAACCGAAAGGTCCAGAGTtttt                                                                                            >  1:2150366/1‑97 (MQ=255)
       tatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTg                                                                                       <  1:1139024/100‑1 (MQ=255)
       tatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTg                                                                                       <  2:1770533/100‑1 (MQ=255)
       tatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTg                                                                                       >  2:1072264/1‑100 (MQ=255)
       tatCCCACCGTGCGGGACTGAACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTTAGGCCCCCGCACCGAAAGGTCCGGGGTTtttgtttg                                                                                       <  1:774184/100‑1 (MQ=255)
        atCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGa                                                                                      <  1:687047/100‑1 (MQ=255)
        atCCCACAGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGa                                                                                      <  1:1865491/100‑1 (MQ=255)
         tCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGAc                                                                                     <  1:1781577/100‑1 (MQ=255)
          cccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGAcc                                                                                    >  2:1245520/1‑100 (MQ=255)
           ccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCt                                                                                   >  1:11782/1‑100 (MQ=255)
           ccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCt                                                                                   >  2:1597418/1‑100 (MQ=255)
            cACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCtt                                                                                  >  2:133246/1‑100 (MQ=255)
            cACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCt                                                                                   <  1:205246/99‑1 (MQ=255)
              ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTaa                                                                                >  1:3445960/1‑100 (MQ=255)
              ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTaa                                                                                <  2:1707257/100‑1 (MQ=255)
                gTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTaaaa                                                                              <  2:459569/100‑1 (MQ=255)
                 tGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTaaaaa                                                                             <  2:1116921/100‑1 (MQ=255)
                      ggCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATaa                                                                        >  2:2997977/1‑100 (MQ=255)
                      ggCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATaa                                                                        >  2:1617063/1‑100 (MQ=255)
                       gCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAAc                                                                       <  2:2926495/100‑1 (MQ=255)
                       gCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAAc                                                                       >  2:721118/1‑100 (MQ=255)
                       gCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGAACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAAc                                                                       <  1:834742/100‑1 (MQ=255)
                        cTGCATTTGGACGAGGAAAGGCGTAGAGATCAAGCCAAAACGAAAAAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAAcc                                                                      <  2:1063196/100‑1 (MQ=255)
                          gCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGATCTTAAAAACATaa                                                                        >  1:2635862/1‑96 (MQ=255)
                           cACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCgag                                                                   >  1:1857693/1‑100 (MQ=255)
                           cACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGATACGAGGATTTTTTTTGACATTAAAAACATAACCgag                                                                   >  2:213139/1‑100 (MQ=255)
                            aCTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCgagg                                                                  <  1:721109/100‑1 (MQ=255)
                            aCTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCgagg                                                                  >  2:2105386/1‑100 (MQ=255)
                             cTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCgagga                                                                 >  1:498127/1‑100 (MQ=255)
                               tGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGGCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGc                                                               <  1:2740383/100‑1 (MQ=255)
                                ggACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCa                                                              <  2:478950/100‑1 (MQ=255)
                                 gACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAg                                                             <  1:1926223/100‑1 (MQ=255)
                                  aCGAGGAAAGGCTTAGAGATCGAGCCTTAACGAACGAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                            <  2:3048379/100‑1 (MQ=255)
                                    gAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACa                                                          >  1:2780160/1‑100 (MQ=255)
                                      ggAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAAt                                                        <  2:1972403/100‑1 (MQ=255)
                                      ggAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAAt                                                        <  1:2558451/100‑1 (MQ=255)
                                      ggAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAAt                                                        <  2:407025/100‑1 (MQ=255)
                                      ggAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATg                                                       <  1:2133333/100‑1 (MQ=255)
                                       gAAAGGCTTAGAGATCAAGCCGTAACGAACTAAGACCCCCGCACAGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATg                                                       <  2:169868/100‑1 (MQ=255)
                                        aaaGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGa                                                      >  2:2266536/1‑100 (MQ=255)
                                        aaaGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGa                                                      >  1:2684719/1‑100 (MQ=255)
                                         aaGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCGCGCACCGAAAGATCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGaa                                                     <  2:2007714/100‑1 (MQ=255)
                                         aaGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGaa                                                     <  2:3446013/100‑1 (MQ=255)
                                          aGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATa                                                   <  1:1956529/100‑1 (MQ=255)
                                           ggCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACTTTAAAAACATAACCAAGGAGTAGACAATGAATa                                                   >  1:3186495/1‑100 (MQ=255)
                                            gCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTTTTTTTAATTACATAAACGAGGGGCAAAAAATg                                                       >  1:2069241/1‑95 (MQ=255)
                                            gCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATaa                                                  >  2:2825709/1‑100 (MQ=255)
                                             cTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCCCCAAAAGGTCCGGGGTTTTTTTTTGACCTTAAACACATAACCGAGGACCAGACAATGAATAAc                                                 >  1:2241077/1‑100 (MQ=255)
                                              ttAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACa                                                >  2:1581718/1‑100 (MQ=255)
                                               tAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAg                                               <  2:2460689/100‑1 (MQ=255)
                                               tAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAg                                               <  2:2243844/100‑1 (MQ=255)
                                               tAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGCATCAGACAATGAATAACAg                                               >  1:742541/1‑100 (MQ=255)
                                                agagATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGc                                              >  2:2397922/1‑100 (MQ=255)
                                                agagATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGc                                              <  1:1376201/100‑1 (MQ=255)
                                                 gagaTCAAGCCTTAACGAACTAAGGCCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGca                                             <  2:365891/100‑1 (MQ=255)
                                                     tCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACaaa                                         >  2:2142251/1‑100 (MQ=255)
                                                       aaGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAAt                                       <  1:1650167/100‑1 (MQ=255)
                                                       aaGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAAt                                       <  2:3339336/100‑1 (MQ=255)
                                                       aaGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAAt                                       <  1:138483/100‑1 (MQ=255)
                                                         gCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTc                                     >  2:3143739/1‑100 (MQ=255)
                                                           cTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTg                                   <  2:2225887/100‑1 (MQ=255)
                                                           cTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTg                                   >  2:300411/1‑100 (MQ=255)
                                                            ttAACGAACTAAGACCCCCGCGCCTAATGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCt                                    <  2:1917050/98‑1 (MQ=255)
                                                            ttAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTg                                   <  1:2612107/100‑1 (MQ=255)
                                                            ttAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTg                                   <  2:3364788/99‑1 (MQ=255)
                                                              aaCGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTCAAAACATAACCGAGGAGCAGACACTGAATAACAGCTCAAAATTCTCtt                                 >  1:2415277/1‑99 (MQ=255)
                                                              aaCGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGttt                                >  1:2298300/1‑100 (MQ=255)
                                                              aaCGAACTAAGACCCCCGCACCGAAAGGTCCCGGGTTTGTTCTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGtt                                 <  1:2437218/99‑1 (MQ=255)
                                                              aaCGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACTGCACAAAATTCTGTTtc                               <  2:1002799/100‑1 (MQ=255)
                                                                cGAACTAAGACCCCCGCACCGAAAGGTCCGGGGCTTTTTTTTTTCCTTTAAAAAATAACCGACGCGCAGACAATAAATAACTGCACAACATTCAGTTtct                              >  1:1626792/1‑100 (MQ=255)
                                                                    cTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAg                          <  1:120134/100‑1 (MQ=255)
                                                                       aGACCCCCGCACCAAAAGGCCCGGGCTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCaa                           <  1:2397889/96‑1 (MQ=255)
                                                                        gACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTc                      >  1:3404908/1‑100 (MQ=255)
                                                                        gACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCAAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTc                      <  2:715629/100‑1 (MQ=255)
                                                                          cccccGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCa                     <  1:812012/99‑1 (MQ=255)
                                                                          cccccGCACCAAAAGGTCCGGGGTTTTTAAATAACCAAAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTc                      <  1:2825665/98‑1 (MQ=255)
                                                                           ccccGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAgg                   >  1:538956/1‑100 (MQ=255)
                                                                            cccGCACCGAAAGGTCCGGGGTTTTTTTTTTTTTTTTAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCa                     >  1:2629691/1‑97 (MQ=255)
                                                                            cccGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGCGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGa                  >  1:538297/1‑100 (MQ=255)
                                                                            cccGCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAgg                   <  1:1942405/99‑1 (MQ=255)
                                                                               gCACCGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACgg               <  2:1780223/100‑1 (MQ=255)
                                                                                  ccGAAAGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGACTAACAGCACAAAATTCTGTTTCTCAAGATTCAGCAc                 >  1:497206/1‑95 (MQ=255)
                                                                                     aaaGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaact         <  1:2608331/100‑1 (MQ=255)
                                                                                     aaaGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaact         <  1:1695645/100‑1 (MQ=255)
                                                                                       aGGTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaacta        <  1:3299612/99‑1 (MQ=255)
                                                                                         gTCCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGGTTCTCAAGATTCAGGACGGGGaactaact     >  2:1835997/1‑100 (MQ=255)
                                                                                           gcGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaactaact     <  1:1152317/97‑1 (MQ=255)
                                                                                           ccGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTAt   >  2:3306662/1‑100 (MQ=255)
                                                                                           ccGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTAt   <  1:2105355/100‑1 (MQ=255)
                                                                                            cGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATg  >  2:293354/1‑100 (MQ=255)
                                                                                                  |                                                                                              
TGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATG  >  NC_000913/3950371‑3950562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: