Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,152,760 G→T N113K (AAC→AAA ppc ← phosphoenolpyruvate carboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,152,7600GT96.2% 348.5 / ‑0.4 106N113K (AAC→AAAppcphosphoenolpyruvate carboxylase
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base T (53/49);  total (56/50)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

TACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGGTGTTGGC  >  NC_000913/4152663‑4152855
                                                                                                 |                                                                                               
tACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTCCTTTTGTGATGGTGCCTTCGCTCAGTTCCGGCTGttt                                                                                               >  1:2342812/1‑100 (MQ=255)
   gacgGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGtttttt                                                                                            >  2:1114772/1‑100 (MQ=255)
    acgGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTc                                                                                           <  1:2268062/100‑1 (MQ=255)
    acgGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTc                                                                                           <  2:1496500/100‑1 (MQ=255)
     cgGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCa                                                                                          <  1:380333/100‑1 (MQ=255)
      gggTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAg                                                                                         <  1:2329339/100‑1 (MQ=255)
       ggTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGTTTTTTTTTTCGCTCAGTTCCGGCTGTTTTTTCAGt                                                                                        >  1:2041847/1‑100 (MQ=255)
       ggTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAg                                                                                         <  1:3153447/99‑1 (MQ=255)
       ggTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGt                                                                                        >  1:1488126/1‑100 (MQ=255)
       ggTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTCTTCAGt                                                                                        <  1:1747628/100‑1 (MQ=255)
        gTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGtt                                                                                       >  1:2186777/1‑100 (MQ=255)
         tAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGttt                                                                                      <  2:2784417/100‑1 (MQ=255)
         tAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGttt                                                                                      <  1:3117174/100‑1 (MQ=255)
           aTTTCGGTTGGGTGAGCCGTGAGGACCAGTTCAAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTAc                                                                                    >  2:3297528/1‑100 (MQ=255)
             ttCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGc                                                                                  <  1:3416188/100‑1 (MQ=255)
             ttCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGc                                                                                  >  2:3124238/1‑100 (MQ=255)
               cGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAg                                                                                <  1:2519711/100‑1 (MQ=255)
               cGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAg                                                                                <  1:406132/100‑1 (MQ=255)
                 gTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAggg                                                                              <  2:1041532/100‑1 (MQ=255)
                   tGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTg                                                                            >  1:1585777/1‑100 (MQ=255)
                    gggTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGc                                                                           >  2:3195157/1‑100 (MQ=255)
                    gggTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTAAGTTAAGGCTGTTTTTTCAGTTTAAGGAGGGTGc                                                                           >  1:3112376/1‑100 (MQ=255)
                      gTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCgg                                                                         >  1:2508590/1‑100 (MQ=255)
                      gTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCgg                                                                         >  1:1475595/1‑100 (MQ=255)
                       tGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCggg                                                                        <  2:2893403/100‑1 (MQ=255)
                        gAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGc                                                                       <  1:177413/100‑1 (MQ=255)
                         aGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCg                                                                      >  2:3213736/1‑100 (MQ=255)
                          gCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCg                                                                      >  1:2250312/1‑99 (MQ=255)
                          gCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGa                                                                     >  1:3162410/1‑100 (MQ=255)
                          gCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGa                                                                     >  2:2432120/1‑100 (MQ=255)
                           ccGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGAt                                                                    <  1:1273228/100‑1 (MQ=255)
                            cGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATc                                                                   >  2:1854141/1‑100 (MQ=255)
                             gTGAGGACCAGTTCCAGCGACAGCGATTCCATTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGACTGTTTTTTAAGTTTACGCAGTGTGCGGGCg                                                                      >  1:2830230/1‑96 (MQ=255)
                             gTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCa                                                                  <  1:1057079/100‑1 (MQ=255)
                               gAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACt                                                                >  1:1640555/1‑100 (MQ=255)
                                 ggACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTc                                                              >  1:470744/1‑100 (MQ=255)
                                 ggACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTAATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTTCGCAGGGTGCGGGCGATCACTTc                                                              >  1:1852584/1‑100 (MQ=255)
                                  gACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTTTTTTTTTTCAGGGTGCGGGCGATCACttac                                                             >  1:1919633/1‑98 (MQ=255)
                                   aCCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTc                                                              >  1:3284545/1‑98 (MQ=255)
                                    ccAGTTCCAGTGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCgg                                                           <  2:2959610/100‑1 (MQ=255)
                                    ccAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCgg                                                           >  2:3476356/1‑100 (MQ=255)
                                       gTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGtt                                                        >  1:3007432/1‑100 (MQ=255)
                                         tCCAGCGACAGCGATTCCACTGCTTTTTTTATGGTGTCTTCGCTCAGTTCCGGCTGTTTCTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGc                                                      >  1:2028309/1‑100 (MQ=255)
                                         tCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTga                                                      <  2:1179918/100‑2 (MQ=255)
                                            agcgacagcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTgg                                                   <  2:235069/100‑1 (MQ=255)
                                            agcgacagcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTgg                                                   <  2:3323900/100‑1 (MQ=255)
                                             gcgacagcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGc                                                  <  2:1310895/100‑1 (MQ=255)
                                              cgacagcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCa                                                 >  1:172223/1‑100 (MQ=255)
                                               gacagcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAg                                                <  1:1465127/100‑1 (MQ=255)
                                                 cagcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCt                                              >  2:1283846/1‑100 (MQ=255)
                                                 cagcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCt                                              >  2:2152611/1‑100 (MQ=255)
                                                  agcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCtt                                             <  2:2925853/100‑1 (MQ=255)
                                                  agcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCtt                                             <  2:2925794/100‑1 (MQ=255)
                                                   gcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTc                                            <  1:1212638/100‑1 (MQ=255)
                                                   gcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTc                                            >  2:2218566/1‑100 (MQ=255)
                                                   gcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTc                                            <  2:621792/100‑1 (MQ=255)
                                                    cgaTTCCACTGCTTTTTTTATGATGTCTTCGCTCAGTTCCGGCTGGTATATCAGTTTACGCAGCAGGCGGTCGATCACTTCCGGATTGCTGGCAGCTTCg                                           >  1:237315/1‑100 (MQ=255)
                                                    cgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTTTTTTTTCGTAGGGTGCGGGCGCTCACTTCCGGGTTGCTGGCAGCTTCg                                           >  1:1593486/1‑100 (MQ=255)
                                                    cgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCg                                           >  1:1993361/1‑100 (MQ=255)
                                                     gaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCg                                           >  1:2796272/1‑99 (MQ=255)
                                                     gaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGc                                          >  1:2331754/1‑100 (MQ=255)
                                                      aTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGcc                                         <  1:2894239/100‑1 (MQ=255)
                                                       ttCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCt                                        <  2:1983880/100‑1 (MQ=255)
                                                        tCCACTGCTTTTTTTATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGcc                                         <  1:1712805/98‑1 (MQ=255)
                                                         ccACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCttt                                      >  1:1112089/1‑100 (MQ=255)
                                                          cACTGCTTTTTTGATTTTTTCTTCGATCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTc                                     >  1:1336846/1‑100 (MQ=255)
                                                          cACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTc                                     >  2:1732680/1‑100 (MQ=255)
                                                          cACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTc                                     <  1:815305/100‑1 (MQ=255)
                                                            cTGCTTTTTTTTTTTTTTTTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTATGCAGGGTGCGGGCGATGGCTTCCGTGTTGCTGGCAGCttttttttt                                      >  1:1014622/1‑90 (MQ=255)
                                                            cTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCgg                                   <  1:2268332/100‑1 (MQ=255)
                                                            cTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCgg                                   >  1:1733068/1‑100 (MQ=255)
                                                            cTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCgg                                   >  2:2434445/1‑100 (MQ=255)
                                                              gCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCg                                 >  2:602867/1‑100 (MQ=255)
                                                               cTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGa                                >  2:2751200/1‑100 (MQ=255)
                                                                       aTGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCtgt                        >  1:1231076/1‑100 (MQ=255)
                                                                       aTGGTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCtgt                        >  2:2796558/1‑100 (MQ=255)
                                                                         ggTGTCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTgg                      <  2:1594991/100‑1 (MQ=255)
                                                                          gtgtCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGt                     <  2:2729503/100‑1 (MQ=255)
                                                                           tgtCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTa                    <  1:3002925/100‑1 (MQ=255)
                                                                             tCTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTAtt                  <  1:2677829/100‑1 (MQ=255)
                                                                              cTTCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTg                 <  1:1072497/100‑1 (MQ=255)
                                                                               ttCGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGc                >  1:3304754/1‑100 (MQ=255)
                                                                                 cGCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTc              >  1:2762666/1‑100 (MQ=255)
                                                                                  gCTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTcg             >  2:2608467/1‑100 (MQ=255)
                                                                                   cTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTcgg            >  2:823972/1‑100 (MQ=255)
                                                                                   cTCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTcgg            >  2:813683/1‑100 (MQ=255)
                                                                                    tCAGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTcggc           <  1:2405275/100‑1 (MQ=255)
                                                                                      aGTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTcggcgg         >  2:2081146/1‑100 (MQ=255)
                                                                                       gTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTcggcgg         <  1:841639/99‑1 (MQ=255)
                                                                                       gTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTcggcgg         >  2:624829/1‑99 (MQ=255)
                                                                                       gTTCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGgt        <  2:1500725/100‑1 (MQ=255)
                                                                                       gTTCCGGCTGTTTTTGGGGTTTACGCAGGGGGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGgt        <  1:1164380/100‑1 (MQ=255)
                                                                                        ttCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGgtg       <  2:1564273/100‑1 (MQ=255)
                                                                                         tCCGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGgtgt      <  1:230150/100‑1 (MQ=255)
                                                                                          ccGGCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGgtgt      <  1:2732706/99‑1 (MQ=255)
                                                                                            ggCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGGTGTTgg   <  1:899645/100‑1 (MQ=255)
                                                                                            ggCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGGTGTTgg   <  1:233797/100‑1 (MQ=255)
                                                                                            ggCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGGTGTTgg   >  2:1622638/1‑100 (MQ=255)
                                                                                            ggCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGGTGTTgg   >  2:1848556/1‑100 (MQ=255)
                                                                                             gCTGTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGGTGTTGGc  >  2:60927/1‑100 (MQ=255)
                                                                                                 |                                                                                               
TACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGGTGTTGGC  >  NC_000913/4152663‑4152855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: