Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,168,540 T→A intergenic (+93/‑101) gltT → / → rrlB tRNA‑Glu/23S ribosomal RNA of rrnB operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,168,5400TA98.8% 437.1 / ‑4.5 167intergenic (+93/‑101)gltT/rrlBtRNA‑Glu/23S ribosomal RNA of rrnB operon
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base A (81/84);  total (83/84)
Fisher's exact test for biased strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 167 total aligned reads displayed.

CGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTT  >  NC_000913/4168443‑4168636
                                                                                                 |                                                                                                
cGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACa                                                                                                >  2:1417267/1‑100 (MQ=255)
    aCTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCt                                                                                            <  2:3267261/100‑1 (MQ=255)
    aCTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCt                                                                                            >  1:14081/1‑100 (MQ=255)
     cTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCtn                                                                                           <  2:869563/100‑2 (MQ=255)
     cTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTg                                                                                           <  2:3142949/100‑1 (MQ=255)
        gCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGaaa                                                                                        >  2:2317513/1‑100 (MQ=255)
          tGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAAt                                                                                      >  1:3348571/1‑100 (MQ=255)
           ggTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGTGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAAtt                                                                                     <  1:2292178/100‑1 (MQ=255)
           ggTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAAtt                                                                                     >  2:210720/1‑100 (MQ=255)
             tttGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGa                                                                                   <  1:1017461/100‑1 (MQ=255)
             tttGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGa                                                                                   <  2:2597264/100‑1 (MQ=255)
              ttGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTGAAAATCTGGAACAAGCTGTAAATTGaa                                                                                  >  1:1135048/1‑100 (MQ=255)
              ttGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGaa                                                                                  <  1:2668469/100‑1 (MQ=255)
              ttGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGaa                                                                                  <  2:1643497/100‑1 (MQ=255)
               tgtgAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGaaa                                                                                 >  1:2697337/1‑100 (MQ=255)
               tgtgAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGaaa                                                                                 <  2:2683828/100‑1 (MQ=255)
               tgtgAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGaaa                                                                                 <  1:1167714/100‑1 (MQ=255)
                gtgagtgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAac                                                                                <  1:2189048/100‑1 (MQ=255)
                gtgagtgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAac                                                                                >  1:3009276/1‑100 (MQ=255)
                    gtgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTg                                                                            <  2:2035044/100‑1 (MQ=255)
                    gtgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTg                                                                            <  1:3444738/100‑1 (MQ=255)
                    gtgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTg                                                                            <  2:304201/100‑1 (MQ=255)
                    gtgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTCAAAAATCTGGAACAAGCTGAAAATTGAAACACTg                                                                            >  1:1831673/1‑100 (MQ=255)
                      gaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGaa                                                                          <  2:612523/100‑1 (MQ=255)
                       aaaGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGaac                                                                         >  2:1657429/1‑100 (MQ=255)
                       aaaGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGaac                                                                         <  1:453554/100‑1 (MQ=255)
                       aaaGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGaac                                                                         <  2:1005975/100‑1 (MQ=255)
                       aaaGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGaac                                                                         <  2:2148750/100‑1 (MQ=255)
                        aaGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGaaca                                                                        >  2:1571903/1‑100 (MQ=255)
                          gTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGaacaac                                                                      <  1:2001928/100‑1 (MQ=255)
                          gTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGaacaac                                                                      <  2:778782/100‑1 (MQ=255)
                            cGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACga                                                                    >  1:3251947/1‑100 (MQ=25)
                            cGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACga                                                                    <  1:1402975/100‑1 (MQ=25)
                             gCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACgag                                                                   >  2:1624062/1‑100 (MQ=25)
                             gCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACgag                                                                   <  1:2831813/100‑1 (MQ=25)
                             gCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACgag                                                                   >  2:511458/1‑100 (MQ=25)
                                gACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAgt                                                                <  2:2520988/100‑1 (MQ=25)
                                  ccTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAgttg                                                              >  2:228031/1‑100 (MQ=17)
                                     tAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTc                                                           >  1:759799/1‑100 (MQ=17)
                                      aaTATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCg                                                          >  1:1952955/1‑100 (MQ=18)
                                        tatCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTg                                                        <  2:1797252/100‑1 (MQ=18)
                                        tatCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTg                                                        <  2:3441106/100‑1 (MQ=17)
                                        tatCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTg                                                        <  1:1070545/100‑1 (MQ=18)
                                         atCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGa                                                       >  1:2418741/1‑100 (MQ=17)
                                         atCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGa                                                       >  1:3006895/1‑100 (MQ=18)
                                          tctcAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAg                                                      >  2:2261945/1‑100 (MQ=17)
                                          tctcAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAAGCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAg                                                      <  1:3336840/100‑1 (MQ=12)
                                           ctcAAAACTCATCTTCGGGTGATGTTTGAGTTATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGt                                                     >  2:2604611/1‑100 (MQ=255)
                                           ctcAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAg                                                      >  2:3301325/1‑99 (MQ=18)
                                           ctcAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAg                                                      <  2:1609264/99‑1 (MQ=18)
                                           ctcAAAACTCATCTTCGGGTAATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGt                                                     >  2:54930/1‑100 (MQ=255)
                                             cAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGtct                                                   >  2:3024807/1‑100 (MQ=17)
                                             cAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGtct                                                   >  1:3034700/1‑100 (MQ=17)
                                             cAAAACTCATCTTCGGGTGATGTTTGAGAGATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGtct                                                   <  1:2094091/100‑1 (MQ=255)
                                                  cTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCaa                                              <  1:678953/100‑1 (MQ=18)
                                                   tCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCaaa                                             <  2:1122972/100‑1 (MQ=18)
                                                     aTCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAAtt                                           <  1:1621328/100‑1 (MQ=17)
                                                       cTTCGGGTGATGTTTGAGATATTTGCTCTTTAACAATCTGGATCAAGCTGAAAATTGGAACACTGAACAAGGAGAGGTGTTTGTGAGTGTCTCAAAtttt                                         >  1:2083382/1‑100 (MQ=11)
                                                       cTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAAtttt                                         >  2:932350/1‑100 (MQ=18)
                                                        ttCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTc                                        <  2:1952984/100‑1 (MQ=18)
                                                        ttCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTc                                        <  2:960089/100‑1 (MQ=18)
                                                        atctcaaacagTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTc                                        <  2:1814257/90‑1 (MQ=11)
                                                         tCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCg                                       <  2:2718143/100‑1 (MQ=17)
                                                                 tGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACga                               >  1:1696741/1‑100 (MQ=18)
                                                                 tGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACga                               >  1:2775464/1‑100 (MQ=17)
                                                                  gTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACgat                              >  2:1834144/1‑100 (MQ=18)
                                                                   tttGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACgatg                             <  1:1633153/100‑1 (MQ=18)
                                                                   tttGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACgatg                             <  1:3456483/100‑1 (MQ=17)
                                                                    ttGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACgatga                            <  1:3301274/100‑1 (MQ=18)
                                                                    ttGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACgatga                            >  2:374432/1‑100 (MQ=18)
                                                                     tGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACgatgat                           <  2:3151186/100‑1 (MQ=18)
                                                                      gagaTATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACgatg                             <  1:2761745/97‑1 (MQ=17)
                                                                      gagaTATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGatgatg                          <  1:726855/100‑1 (MQ=18)
                                                                          tatTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATc                      >  1:2507114/1‑100 (MQ=18)
                                                                          tatTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATc                      <  1:1126825/100‑1 (MQ=18)
                                                                          tatTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATc                      <  1:31158/100‑1 (MQ=18)
                                                                          tatTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATc                      >  1:617258/1‑100 (MQ=18)
                                                                           atTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCg                     >  2:534429/1‑100 (MQ=17)
                                                                             ttGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaa                   >  2:2627297/1‑100 (MQ=18)
                                                                             ttGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaa                   >  2:1395318/1‑100 (MQ=18)
                                                                             ttGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaa                   >  1:13065/1‑100 (MQ=17)
                                                                             ttGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaa                   >  1:1015977/1‑100 (MQ=17)
                                                                               gCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaag                 <  2:250342/100‑1 (MQ=18)
                                                                               gCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaag                 >  1:1363011/1‑100 (MQ=17)
                                                                                ctctTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaaga                >  1:1481283/1‑100 (MQ=18)
                                                                                 tctTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCaaaagaa               >  2:296763/1‑100 (MQ=12)
                                                                                  ctTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaagaaa              >  1:2909663/1‑100 (MQ=18)
                                                                                  ctTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaagaaa              >  2:1482005/1‑100 (MQ=18)
                                                                                  ctTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaagaaa              >  1:885966/1‑100 (MQ=17)
                                                                                   tttAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAAc             <  2:1228644/100‑1 (MQ=18)
                                                                                     tAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACa            <  1:282754/99‑1 (MQ=17)
                                                                                     tAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACAt           >  2:2418774/1‑100 (MQ=18)
                                                                                        aaaTCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCtt        <  1:3387135/100‑1 (MQ=17)
                                                                                          aTCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCg      >  1:1306860/1‑100 (MQ=18)
                                                                                          aTCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCg      >  1:2163385/1‑100 (MQ=18)
                                                                                          aTCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCg      >  2:2904745/1‑100 (MQ=18)
                                                                                          aTCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCg      <  2:3284750/100‑1 (MQ=18)
                                                                                            cTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCggg    <  2:820961/100‑1 (MQ=18)
                                                                                              ggAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGtt  >  1:238936/1‑100 (MQ=18)
                                                                                              ggAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGtt  >  2:1739680/1‑100 (MQ=18)
                                                                                                 |                                                                                                
CGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTT  >  NC_000913/4168443‑4168636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: