Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 265,418 A→G R42R (CGT→CGC)  ykfF ← CP4‑6 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913265,4180AG99.3% 484.8 / NA 139R42R (CGT→CGC) ykfFCP4‑6 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (59/78);  total (60/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 140 total aligned reads displayed.

CTGTACGGATGCCTGAGGTGCGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCACGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAG  >  NC_000913/265321‑265512
                                                                                                 |                                                                                              
cTGTACGGATGCCTGAGGTGCGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATcgcg                                                                                              >  2:257934/1‑100 (MQ=255)
  gTACGGATGCCTGAGGTGCGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGaa                                                                                            >  1:2464087/1‑100 (MQ=255)
    aCGGATGCCTGAGGTGCGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAacc                                                                                          <  1:2319161/100‑1 (MQ=255)
     cGGATGCCTGAGGTGCGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAacca                                                                                         >  2:2744021/1‑100 (MQ=255)
      ggATGCCTGAGGTGCGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAaccac                                                                                        <  2:2063783/100‑1 (MQ=255)
      ggATGCCTGAGGTGCGGATATAAAGGATAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCAGGCCTTCAGTATCCCGAacccc                                                                                        >  1:1309762/1‑98 (MQ=21)
        aTGCCTGAGGTGCGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAAccacca                                                                                      <  2:3037381/100‑1 (MQ=255)
        aTGCCTGAGGTGCGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAAccacca                                                                                      <  2:1088862/100‑1 (MQ=255)
           ccTGAGGTGCGGATATAGCGGTTAAGACATTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGAc                                                                                   >  2:2421965/1‑100 (MQ=255)
              gAGGTGCGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGa                                                                                >  2:2543184/1‑100 (MQ=255)
              gAGGTGCGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGa                                                                                >  1:513672/1‑100 (MQ=255)
                  tGCGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTg                                                                            >  2:1538832/1‑100 (MQ=255)
                    cGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGAc                                                                          <  2:3359946/100‑1 (MQ=255)
                      gATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTg                                                                        >  1:660614/1‑100 (MQ=255)
                       atatAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGc                                                                       <  1:942751/100‑1 (MQ=255)
                        tataGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGcc                                                                      >  2:3402363/1‑100 (MQ=255)
                         ataGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGccc                                                                     <  2:1334726/100‑1 (MQ=255)
                         ataGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGccc                                                                     <  2:278902/100‑1 (MQ=255)
                          taGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCt                                                                    >  1:556272/1‑100 (MQ=255)
                           aGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTg                                                                   >  2:3060084/1‑100 (MQ=255)
                           aGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTg                                                                   >  2:2075564/1‑100 (MQ=255)
                           aGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTg                                                                   <  1:3162829/100‑1 (MQ=255)
                            gCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAGGTGCCTGCCCTg                                                                   >  1:2162056/1‑99 (MQ=255)
                            gCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTgg                                                                  >  2:2226738/1‑100 (MQ=255)
                             cGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGgt                                                                 <  1:1236731/100‑1 (MQ=255)
                              ggTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAGGTGACGGCCCTGgtc                                                                >  2:1866823/1‑100 (MQ=255)
                               gTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGgtcg                                                               <  1:845403/100‑1 (MQ=255)
                                  aaGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGgtc                                                                >  1:1509397/1‑96 (MQ=255)
                                  aaGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCt                                                            <  2:2226129/100‑1 (MQ=255)
                                   aGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGACGTGACTGCCCTGGTCGTCtt                                                           >  1:1486273/1‑100 (MQ=255)
                                   aGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCtt                                                           <  1:521835/100‑1 (MQ=255)
                                   aGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCC‑GCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTc                                                          <  2:1956508/100‑1 (MQ=255)
                                    gACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTc                                                          <  2:1812607/100‑1 (MQ=255)
                                    gACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTc                                                          <  1:1908728/100‑1 (MQ=255)
                                    gACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTc                                                          <  1:1677131/100‑1 (MQ=255)
                                     aCCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCg                                                         <  1:1892560/100‑1 (MQ=255)
                                     aCCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCg                                                         <  2:560828/100‑1 (MQ=255)
                                      ccTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGa                                                        >  2:1532973/1‑100 (MQ=255)
                                       cTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAg                                                       >  2:3354358/1‑100 (MQ=255)
                                       cTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAg                                                       <  1:1450931/100‑1 (MQ=255)
                                       cTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAg                                                       >  1:885117/1‑100 (MQ=255)
                                         tCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAgtg                                                     <  2:2081704/100‑1 (MQ=255)
                                         tCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAgtg                                                     <  2:2376487/100‑1 (MQ=255)
                                         tCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAgtg                                                     <  2:474880/100‑1 (MQ=255)
                                          cACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCACGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAgt                                                      <  1:3076540/98‑1 (MQ=255)
                                           aCCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGtgtg                                                   <  2:761561/100‑1 (MQ=255)
                                           aCCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGtgtg                                                   >  1:744144/1‑100 (MQ=255)
                                            ccGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGGCGGAAGTGACTGCCCTGGTCGTCTTCGAGTgaga                                                  >  2:895173/1‑100 (MQ=255)
                                            ccGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGa                                                  <  2:2120834/100‑1 (MQ=255)
                                                cATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGatact                                              >  1:2617215/1‑98 (MQ=255)
                                                cATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATAtt                                              >  2:168405/1‑100 (MQ=255)
                                                cATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCAACAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGatat                                               >  2:1475970/1‑99 (MQ=255)
                                                  tCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGc                                            <  2:1202820/100‑1 (MQ=255)
                                                  tCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGc                                            <  2:3240582/100‑1 (MQ=255)
                                                  tCCGGCTCAAAGTTCCATGCCCGCCAGACCATACGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGc                                            >  2:3299088/1‑100 (MQ=255)
                                                   ccGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGc                                            <  1:2575859/99‑1 (MQ=255)
                                                     ggCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGt                                         <  1:3266014/100‑1 (MQ=255)
                                                      gCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTa                                        >  2:2608657/1‑100 (MQ=255)
                                                       cTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTa                                        <  1:2610080/99‑1 (MQ=255)
                                                        tCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACg                                      >  1:752942/1‑100 (MQ=255)
                                                         cAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGt                                     >  1:3241327/1‑100 (MQ=255)
                                                          aaaGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTg                                    <  1:2572762/100‑1 (MQ=255)
                                                          aaaGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTg                                    <  1:123628/100‑1 (MQ=255)
                                                           aaGTTCCATGCCCGCCAGACCATCCGGCCTTCTGTATCGTGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTgg                                   <  1:2437368/100‑1 (MQ=255)
                                                           aaGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTgg                                   >  2:3253137/1‑100 (MQ=255)
                                                            aGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGt                                  <  2:1445830/100‑1 (MQ=255)
                                                            aGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGt                                  <  2:2045422/100‑1 (MQ=255)
                                                              ttCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAg                                <  2:1031739/100‑1 (MQ=255)
                                                              ttCCATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAg                                <  1:1538810/100‑1 (MQ=255)
                                                                ccATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTg                              <  1:1471811/100‑1 (MQ=255)
                                                                ccATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTg                              >  1:848576/1‑100 (MQ=255)
                                                                 cATGCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGa                             >  2:1810712/1‑100 (MQ=255)
                                                                   tGCCCGCCCGACCATACGGCCTTCAGTATCGAGAAACATCAGACGGCAGCGACTGCGCTGTTGGTCTTCGAGTGTTATATTGCGGTACGTGGTAGTGa                             <  1:638453/98‑1 (MQ=255)
                                                                    gCCCGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACcg                          >  1:498129/1‑100 (MQ=255)
                                                                     cccGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACcgc                         <  1:593255/100‑1 (MQ=255)
                                                                     cccGCCAGACCATCCCGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGAc                            <  1:3402311/97‑1 (MQ=255)
                                                                      ccGCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACcgct                        >  2:3464446/1‑100 (MQ=255)
                                                                        gCCAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACcgcttn                      <  1:708929/100‑2 (MQ=255)
                                                                         ccAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGAcc                           >  1:402418/1‑94 (MQ=255)
                                                                          cAGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACcgcttgcg                    <  2:993200/100‑1 (MQ=255)
                                                                           aGACCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACcgcttgcgc                   <  1:2701368/100‑1 (MQ=255)
                                                                             aCCATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACcgcttgcgctt                 <  2:709699/100‑1 (MQ=255)
                                                                              ccATCCGGCCTTCAGTTTCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACcgcttgcgcttg                <  1:2034583/100‑1 (MQ=255)
                                                                              ccATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACcgcttgcgcttg                <  2:1276775/100‑1 (MQ=255)
                                                                              ccATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACcgcttgcgcttg                <  2:692601/100‑1 (MQ=255)
                                                                              ccATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACcgcttgcgcttg                <  1:2608622/100‑1 (MQ=255)
                                                                              ccATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACcgcttgcgcttg                <  2:245702/100‑1 (MQ=255)
                                                                               cATCCGGCCTTCAGTATCGCTAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACcgcttgcgcttg                <  2:671562/99‑1 (MQ=255)
                                                                               cATCCGGCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGt               >  2:871328/1‑100 (MQ=255)
                                                                                aTCCGGCCTTCAGTATCGCGAACCACCAGACGTAAGTGACTGCCCTGGTCGTGTTCGAGTGTGATATTGAAGTACGTGGTGGGGCCCGCTGCCGCCTGtc              >  1:3204116/1‑100 (MQ=255)
                                                                                aTCCGGCCTTCAGTATCGCGAACCACCAGACGTAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGtc              <  1:1832580/100‑1 (MQ=255)
                                                                                 tCCGGCCTTCACTATCGCGAACCTCCAGACGTAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACcgcttgcgcttg                <  1:402545/97‑1 (MQ=255)
                                                                                    ggCCTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCg          <  2:148599/100‑1 (MQ=255)
                                                                                      ccTTCAGTATCGCGTACCACCAGACGGAAGTGTCTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCggg        <  1:1686049/100‑1 (MQ=255)
                                                                                      ccTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCggg        >  1:940849/1‑100 (MQ=255)
                                                                                       cTTCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGt       <  2:915800/100‑1 (MQ=255)
                                                                                        ttCAGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGCGTGAGATATTGCTGTACGTGGGAGGGACCGCTTGCGCTGGTCGCCggg        >  1:2895556/1‑98 (MQ=255)
                                                                                           aGTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGCGTGATATTGCTGTACGCGGTAGTGCCCGCTTGCGCATGTCTCCGGGTGaaa   >  1:3052088/1‑100 (MQ=255)
                                                                                            gTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAg  <  1:2963428/100‑1 (MQ=255)
                                                                                            gTATCGCGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAg  <  2:3098770/100‑1 (MQ=255)
                                                                                                 |                                                                                              
CTGTACGGATGCCTGAGGTGCGGATATAGCGGTTAAGACCTTCACCGGCATCCGGCTCAAAGTTCCATGCCCGCCAGACCATCCGGCCTTCAGTATCACGAACCACCAGACGGAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAG  >  NC_000913/265321‑265512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑