Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,238,726 A→G I365V (ATT→GTT)  yjbH → DUF940 family extracellular polysaccharide protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,238,7260AG95.5% 368.4 / 1.4 112I365V (ATT→GTT) yjbHDUF940 family extracellular polysaccharide protein
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base G (60/47);  total (63/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

CGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCATTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTGGGCCACGAAA  >  NC_000913/4238629‑4238822
                                                                                                 |                                                                                                
cGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGtt                                                                                                >  2:314037/1‑100 (MQ=255)
 ggCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCGAAACGATCCGCGTTa                                                                                               >  2:3417984/1‑100 (MQ=255)
 ggCGAGCAGGTGAAATATCGTGATTCGCGCAAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTa                                                                                               >  1:1429513/1‑100 (MQ=255)
   cGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACg                                                                                             <  2:1923144/100‑1 (MQ=255)
    gAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACgg                                                                                            <  2:2673129/100‑1 (MQ=255)
       cAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGaaa                                                                                         >  2:351734/1‑100 (MQ=255)
         ggTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATTGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAAt                                                                                       >  1:1310348/1‑100 (MQ=255)
         ggTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAAt                                                                                       >  1:1077940/1‑100 (MQ=255)
         ggTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAAt                                                                                       >  1:3024700/1‑100 (MQ=255)
          gTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAAt                                                                                       >  1:2205768/1‑99 (MQ=255)
            gAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGc                                                                                    <  2:3351661/100‑1 (MQ=255)
            gAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGc                                                                                    <  1:673757/100‑1 (MQ=255)
             aaaTATCGTGATTCGCGCGAAGGGATCATCAGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGc                                                                                    >  2:2602285/1‑99 (MQ=255)
              aaTATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCt                                                                                  <  1:447721/100‑1 (MQ=255)
                 atCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTaa                                                                               >  1:2542301/1‑100 (MQ=255)
                  tCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAAc                                                                              <  2:281818/100‑1 (MQ=255)
                  tCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAAc                                                                              <  2:1508789/100‑1 (MQ=255)
                  tCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAAc                                                                              <  2:893284/100‑1 (MQ=255)
                   cGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATTCGCGTTACGGAAAATCGCCTTcaca                                                                             >  1:1237980/1‑100 (MQ=255)
                      gATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCATTACGGAAAATCGCCTTAACATGc                                                                          >  1:1076127/1‑100 (MQ=255)
                        ttCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAACACGATCCGCGTTACGGAAAATCGCCTTAACATGCCg                                                                        <  2:2449682/100‑1 (MQ=255)
                           gcgcgAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCa                                                                      >  1:1045472/1‑99 (MQ=255)
                            cgcgAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAgg                                                                    >  1:2477571/1‑100 (MQ=255)
                             gcgAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGt                                                                   >  1:965663/1‑100 (MQ=255)
                             gcgAAGGGATCATCCGTGCGAATCGGATCGTCATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGt                                                                   <  1:2336144/100‑1 (MQ=255)
                              cgAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTg                                                                  >  1:405926/1‑100 (MQ=255)
                                aaGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTgac                                                                <  2:1811766/100‑1 (MQ=255)
                                  gggATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTgacga                                                              >  1:344995/1‑100 (MQ=255)
                                    gATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGacgacg                                                            >  1:1436505/1‑100 (MQ=255)
                                     atcatcCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACgg                                                           <  1:2555375/100‑1 (MQ=255)
                                     atcatcCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACgg                                                           >  2:2788245/1‑100 (MQ=255)
                                      tcatcCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGa                                                          >  1:2056051/1‑100 (MQ=255)
                                         tcCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAAc                                                       <  2:1897726/100‑1 (MQ=255)
                                          ccggccGATTCGGATCGTGATAAACATTCTGCCGGATGGAATCAAACCGCTCCGGGTTACGGAAAATCGCCTAAAAATGCCCCAGTTGACGACGGaa                                                         <  1:817939/92‑1 (MQ=255)
                                          ccGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAAcc                                                      >  1:2473871/1‑100 (MQ=255)
                                          ccGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAAcc                                                      >  1:1739693/1‑100 (MQ=255)
                                          ccGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAAcc                                                      <  1:468674/100‑1 (MQ=255)
                                          ccGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAAcc                                                      <  2:892770/100‑1 (MQ=255)
                                           cGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCg                                                     >  1:2040804/1‑100 (MQ=255)
                                           cGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCg                                                     <  2:2773497/100‑1 (MQ=255)
                                            gTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGa                                                    <  1:120700/100‑1 (MQ=255)
                                            gTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGa                                                    <  1:58748/100‑1 (MQ=255)
                                            gTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGa                                                    <  1:3062645/100‑1 (MQ=255)
                                            gTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGa                                                    >  2:2707271/1‑100 (MQ=255)
                                            gTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGa                                                    <  1:3006344/100‑1 (MQ=255)
                                              gCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATg                                                  <  2:1998688/100‑1 (MQ=255)
                                               cGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGt                                                 >  2:84997/1‑100 (MQ=255)
                                               cGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGt                                                 <  2:1261809/100‑1 (MQ=255)
                                                gAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTc                                                >  2:425133/1‑100 (MQ=255)
                                                    cGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCa                                            <  1:310211/100‑1 (MQ=255)
                                                    cGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCa                                            >  1:2067469/1‑100 (MQ=255)
                                                     ggATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAg                                           >  2:2465618/1‑100 (MQ=255)
                                                      gATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGc                                          <  2:262014/100‑1 (MQ=255)
                                                      gATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGc                                          >  2:3084024/1‑100 (MQ=255)
                                                      gATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGc                                          >  1:1442974/1‑100 (MQ=255)
                                                      gATCGTGATGAACGATCTGACGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGc                                          <  2:370826/100‑1 (MQ=255)
                                                      gATCGGGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGc                                          <  2:2396888/100‑1 (MQ=255)
                                                       aTCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGcc                                         <  1:109851/100‑1 (MQ=255)
                                                          gTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGa                                      >  2:3127144/1‑100 (MQ=255)
                                                          gTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGa                                      >  2:599108/1‑100 (MQ=255)
                                                          gTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGa                                      >  2:2009879/1‑100 (MQ=255)
                                                           tgatgaACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCGTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGaa                                     >  1:3204583/1‑100 (MQ=255)
                                                              tgaACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGCCGGAAACCGATGTCGCCAGCCTGaaaaa                                  >  2:2485800/1‑100 (MQ=255)
                                                              tgaACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCCGCCTGaaaaa                                  >  2:3387196/1‑100 (MQ=255)
                                                              tgaACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGaaaaa                                  <  2:559497/100‑1 (MQ=255)
                                                               gaACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAat                                 <  2:499532/100‑1 (MQ=255)
                                                                 aCGATCTGCCGGATGGGCTCAAAACGATCCGCATTACGGCAACGCGCCGTGACGGGCCGCAGGAGACAACGGACACCGAtcccg                                               >  1:2929463/1‑80 (MQ=255)
                                                                 aCGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAatca                               <  1:1039751/100‑1 (MQ=255)
                                                                 aCGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAatca                               >  2:36390/1‑100 (MQ=255)
                                                                   gATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAatcatc                             <  2:2764436/100‑1 (MQ=255)
                                                                    aTCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCAtct                            <  1:1676927/100‑1 (MQ=255)
                                                                    aTCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCAtct                            >  2:2132996/1‑100 (MQ=255)
                                                                        gCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGcc                        >  2:1168782/1‑100 (MQ=255)
                                                                          cGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCgg                      >  2:2038654/1‑100 (MQ=255)
                                                                          cGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCg                       >  2:1321791/1‑99 (MQ=255)
                                                                            gATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGgag                    <  2:868008/100‑1 (MQ=255)
                                                                            gATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGgag                    >  2:3135058/1‑100 (MQ=255)
                                                                             aTGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGgaga                   >  1:201259/1‑100 (MQ=255)
                                                                              tGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGagag                  >  2:2099052/1‑100 (MQ=255)
                                                                              tGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGagag                  <  1:2465585/100‑1 (MQ=255)
                                                                                ggATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGcc                <  2:2801492/100‑1 (MQ=255)
                                                                                 gATCAAAACGATCCGCATTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCg               >  1:324728/1‑100 (MQ=255)
                                                                                  aTCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGt              <  2:2723425/100‑1 (MQ=255)
                                                                                    cAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCGGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGt              >  1:2114540/1‑98 (MQ=255)
                                                                                    cAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTg            >  2:2259741/1‑100 (MQ=255)
                                                                                    cAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTg            <  1:2570925/100‑1 (MQ=255)
                                                                                    cAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTg            <  1:362467/100‑1 (MQ=255)
                                                                                    cAAAACGATCCGCATTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTg            <  2:1875130/100‑1 (MQ=255)
                                                                                     aaaaCGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTgg           >  1:866783/1‑100 (MQ=255)
                                                                                      aaaCGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTggg          <  2:107542/100‑1 (MQ=255)
                                                                                      aaaCGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTggg          >  2:360864/1‑100 (MQ=255)
                                                                                      aaaCGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTggg          <  2:3174276/100‑1 (MQ=255)
                                                                                         cGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTGGGCCa       >  1:2887678/1‑100 (MQ=255)
                                                                                          gATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTGGGCCAc      <  2:1670392/100‑1 (MQ=255)
                                                                                          gATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTGGGCCAc      >  2:387041/1‑100 (MQ=255)
                                                                                          gATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTGGGCCAc      >  1:3369285/1‑100 (MQ=255)
                                                                                           aTCCGCGTTACGGAAAATCGCTTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTGGGACACg     <  1:169763/100‑1 (MQ=255)
                                                                                           aTCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTGGGCCACg     >  1:153483/1‑100 (MQ=255)
                                                                                             ccGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTGGGCCACGaa   >  1:258155/1‑100 (MQ=255)
                                                                                              cgcgTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTGGGCCACGaaa  >  2:2071126/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCATTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTGGGCCACGAAA  >  NC_000913/4238629‑4238822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑