BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,340,287	G→A	L79F (CTT→TTT)	adiA ←	arginine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,340,287	0	G	A	98.2%	365.9 / ‑5.3	113	L79F (CTT→TTT)	adiA	arginine decarboxylase
Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (52/59); total (52/61)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

TCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGA  >  NC_000913/4340190‑4340381

tCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGACCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACt                                                                                              >  1:1571745/1‑100 (MQ=255)

 cATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACtt                                                                                             >  2:709258/1‑100 (MQ=255)

  aTCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTa                                                                                            >  2:138827/1‑100 (MQ=255)

  aTCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTa                                                                                            >  2:3055902/1‑100 (MQ=255)

   tCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTAc                                                                                           >  2:1860774/1‑100 (MQ=255)

    cGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTAcc                                                                                          >  1:2858293/1‑100 (MQ=255)

    cGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTAcc                                                                                          >  1:446491/1‑100 (MQ=255)

    cGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTAcc                                                                                          >  2:2487103/1‑100 (MQ=255)

    cGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTAcc                                                                                          <  1:3105923/100‑1 (MQ=255)

     gACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCg                                                                                         >  2:570901/1‑100 (MQ=255)

     gACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCg                                                                                         <  2:1237558/100‑1 (MQ=255)

      aCAAGCTCCAGCAGGTCGCGGTCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGa                                                                                        >  1:2118999/1‑100 (MQ=255)

      aCAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGa                                                                                        >  1:751136/1‑100 (MQ=255)

        aaGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATc                                                                                      <  2:1279156/100‑1 (MQ=255)

         aGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGAt                                                                                       <  1:57811/98‑1 (MQ=255)

         aGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCa                                                                                     <  1:2498919/100‑1 (MQ=255)

          gCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCaa                                                                                    >  1:3220985/1‑100 (MQ=255)

          gCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCaa                                                                                    >  2:1256355/1‑100 (MQ=255)

             ccAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTg                                                                                 <  1:1891437/100‑1 (MQ=255)

                gcagGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCt                                                                              <  2:1438622/100‑1 (MQ=255)

                gcagGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCt                                                                              <  1:3412420/100‑1 (MQ=255)

                     tCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGt                                                                         >  2:120768/1‑100 (MQ=255)

                     tCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGt                                                                         <  1:1465477/100‑1 (MQ=255)

                      cgcgATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGtt                                                                        >  1:2727753/1‑100 (MQ=255)

                      cgcgATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGGTTGTTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGtt                                                                        >  2:1028250/1‑100 (MQ=255)

                        cgATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGtttt                                                                      >  2:3226255/1‑100 (MQ=255)

                         gATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTg                                                                     >  1:1161966/1‑100 (MQ=255)

                           tCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGAt                                                                   >  2:1822107/1‑100 (MQ=255)

                            ccATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATg                                                                  <  2:801784/100‑1 (MQ=255)

                             cATTGCGGCGAGGGCTTTTTCCCGAGCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCGCATGAAACTTACCGATCAATTGTCCGACGTTTTGATGt                                                                 <  1:2146621/100‑1 (MQ=255)

                              aTTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGtt                                                                <  1:3396613/100‑1 (MQ=255)

                              aTTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGtt                                                                <  1:3097269/100‑1 (MQ=255)

                                tGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCg                                                              <  1:1425622/100‑1 (MQ=255)

                                tGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCg                                                              <  2:1633656/100‑1 (MQ=255)

                                  cggcgAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTc                                                            >  2:1776593/1‑100 (MQ=255)

                                  cggcgAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTc                                                            >  2:1776908/1‑100 (MQ=255)

                                   ggcgAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTcc                                                           <  1:3275163/100‑1 (MQ=255)

                                   ggcgAGGGCGTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGTTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATg                                                                  <  1:1227110/93‑1 (MQ=255)

                                    gcgAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCg                                                          <  2:1697566/100‑1 (MQ=255)

                                    gcgAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCg                                                          >  1:80302/1‑100 (MQ=255)

                                     cgAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCgg                                                         <  1:2510712/100‑1 (MQ=255)

                                        gggCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATg                                                      >  1:90650/1‑100 (MQ=255)

                                        gggCTTTTGCCCGGGCGCCCGAGAGGAGGGCCGGCACGTTTTGTTGGCGCTCATGGAACTTCCCGATCAAGTGTCTGACGTTTTGATGTTCGGCCGGa                                                        <  1:3213142/98‑1 (MQ=255)

                                         ggCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGt                                                     >  1:355499/1‑100 (MQ=255)

                                          gCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGtt                                                    >  1:762091/1‑100 (MQ=255)

                                          gCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGtt                                                    >  1:552038/1‑100 (MQ=255)

                                          gCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGtt                                                    >  2:3164293/1‑100 (MQ=255)

                                           cTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTc                                                   >  2:1790691/1‑100 (MQ=255)

                                           cTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCCCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTc                                                   <  2:1896940/100‑1 (MQ=255)

                                            tttttCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTcc                                                  >  2:197484/1‑100 (MQ=255)

                                            tttttCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTcc                                                  <  1:3443942/100‑1 (MQ=255)

                                              tttCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAt                                                >  1:112210/1‑100 (MQ=255)

                                               ttCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAtt                                               <  2:2547145/100‑1 (MQ=255)

                                                tCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAttt                                              <  1:750447/100‑1 (MQ=255)

                                                tCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCAGGAAGTTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAttt                                              <  1:1819679/100‑1 (MQ=255)

                                                 cccGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTg                                             <  1:516497/100‑1 (MQ=255)

                                                 cccGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTACGGATGTTCCAttt                                              >  2:526157/1‑99 (MQ=255)

                                                  ccGATCGCCCAACATGATGACCGGTCCCTTTTGTTGGTGCTTGTGAAGCTTACCCATCAATTGTCTGACGTTTGAATGTTCGTCCGGATGTTCCAttt                                              <  1:1712287/98‑1 (MQ=255)

                                                  ccGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGa                                            <  1:1585789/100‑1 (MQ=255)

                                                  ccGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGa                                            >  1:3153794/1‑100 (MQ=255)

                                                   cGATCGCCCAACAGGAAGACCGGCACTTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGAt                                           <  1:2697509/100‑1 (MQ=255)

                                                    gATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATa                                          <  2:450048/100‑1 (MQ=255)

                                                      tCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGc                                        <  1:138825/100‑1 (MQ=255)

                                                       cGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGc                                        <  1:1790666/99‑1 (MQ=255)

                                                       cGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCt                                       >  1:2280893/1‑100 (MQ=255)

                                                        ncccAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTg                                      >  2:2993193/2‑100 (MQ=255)

                                                        gCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTg                                      <  2:2863300/100‑1 (MQ=255)

                                                        gCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTg                                      <  1:1695809/100‑1 (MQ=255)

                                                        gCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTg                                      >  2:479169/1‑100 (MQ=255)

                                                         cctaaCAGAAAGGCTGGCACGTTTTGTGGGCGATCATGAAACTTTCCGATCAATTGTCTGACGTTTTGATGTTCGTTCGGATGTTCCATTTGATAGCTg                                      <  1:1343380/96‑1 (MQ=255)

                                                         cccAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTg                                      >  2:1524538/1‑99 (MQ=255)

                                                         cccAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGa                                     >  2:2263833/1‑100 (MQ=255)

                                                           cAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAAc                                   >  1:1639084/1‑100 (MQ=255)

                                                              cAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATc                                <  1:2719712/100‑1 (MQ=255)

                                                                ggAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAg                              <  2:1602556/100‑1 (MQ=255)

                                                                ggAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAg                              >  2:1513088/1‑100 (MQ=255)

                                                                ggAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAg                              >  1:1321925/1‑100 (MQ=255)

                                                                    gACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAg                          <  2:52842/100‑1 (MQ=255)

                                                                      ccGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTc                        >  2:2315249/1‑100 (MQ=255)

                                                                          cACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATg                    <  2:2858338/100‑1 (MQ=255)

                                                                           aCGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATgg                   <  2:1035824/100‑1 (MQ=255)

                                                                            cGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGc                  <  2:599076/100‑1 (MQ=255)

                                                                             gTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCt                 <  1:2069130/100‑1 (MQ=255)

                                                                              ttttGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCtt                <  2:1678996/100‑1 (MQ=255)

                                                                                ttgttgGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCg              <  2:3041386/100‑1 (MQ=255)

                                                                                ttgttgGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCg              >  1:1753294/1‑100 (MQ=255)

                                                                                  gttgGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGtt            <  1:1186989/100‑1 (MQ=255)

                                                                                  gttgGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGtt            >  1:3339761/1‑100 (MQ=255)

                                                                                  gttgGCGCTCATGAAACTTACCGATCAATTGTCTGACGTGTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGtt            <  2:1196843/100‑1 (MQ=255)

                                                                                   ttgGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGttt           <  2:388838/100‑1 (MQ=255)

                                                                                       cgcTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATCGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAag       <  1:307785/100‑1 (MQ=255)

                                                                                       cgcTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAag       <  1:2721348/100‑1 (MQ=255)

                                                                                       cgcTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAag       <  2:759122/100‑1 (MQ=255)

                                                                                        gcTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGaa        <  1:3278368/98‑1 (MQ=255)

                                                                                         cTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAagag     >  2:2259064/1‑100 (MQ=255)

                                                                                         cTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAagag     >  2:3287229/1‑100 (MQ=255)

                                                                                           cATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAagagag   <  1:1256338/100‑1 (MQ=255)

                                                                                            aTGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAgagaga  >  1:1565572/1‑100 (MQ=255)

                                                                                            aTGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAgagaga  >  1:2766037/1‑100 (MQ=255)

                                                                                             tGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAgagaga  >  1:201266/1‑99 (MQ=255)

TCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGA  >  NC_000913/4340190‑4340381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`